Isoenzyme analysis of Schistosoma haematobium, S. intercalatum and their hybrids and occurrences of natural hybridization in Cameroon

2003 ◽  
Vol 77 (3) ◽  
pp. 269-274 ◽  
Author(s):  
B.L. Webster ◽  
V.R. Southgate ◽  
L.-A. Tchuem Tchuenté
Keyword(s):  
Isoelectric Focusing ◽  
Schistosoma Haematobium ◽  
Parental Species ◽  
Molecular Data ◽  
Natural Hybridization ◽  
Isoenzyme Analysis ◽  
South West ◽  
Parasitological Survey ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Recombinant Population

AbstractIsoelectric focusing of glucose-6-phosphate dehydrogenase (G6PD) produced clearly identifiable profiles for S. haematobium and S. intercalatum and their hybrids. To provide a more detailed analysis of the interactions of S. haematobium and S. intercalatum in South West Cameroon over the last 12 years, G6PD analyses were carried out on individual schistosomes collected in Kumba in 1990, Loum in 1990, 1999 and 2000 and Barombi Mbo and Barombi Kotto in 1999. Studies were also carried out on the two parental species S. haematobium Barombi Mbo, S. intercalatum Edea and subsequent generations of hybrids resulting from laboratory crosses of the two parental species. The isoenzyme analysis demonstrated that the 1990 isolate from Kumba, was a recombinant of S. intercalatumxS. haematobium, and that 30% of individual schistosomes collected in 1990 in Loum were also recombinants. The remainder gave data indicative of S. haematobium. In 1999, 12.5% of individuals from Loum showed recombination and 10% in 2000. Results from the most recent parasitological survey in October 2000 showed the persistence of the recombinant population in addition to that of S. haematobium. There was also evidence of recombination having taken place in Barombi Kotto but not Barombi Mbo. This study demonstrates how the situation has changed over the last 12 years, and emphasizes the importance of assessing morphological, biological and molecular data together to gain a true picture of the rapidly evolving situation.

Mating interactions of Schistosoma haematobium and S. intercalatum with their hybrid offspring

Parasitology ◽  
2003 ◽  
Vol 126 (4) ◽  
pp. 327-338 ◽  
Author(s):  
B. L. WEBSTER ◽  
V. R. SOUTHGATE
Keyword(s):  
Schistosoma Haematobium ◽  
Parental Species ◽  
Mating Behaviour ◽  
Electrophoretic Analysis ◽  
Hybrid Analysis ◽  
Epidemiological Changes ◽  
Female Hybrid ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Haenszel Test ◽  
Better Than

Experiments were designed to study the mating behaviour between the Schistosoma haematobium [male ]×S. intercalatum [female] hybrid and the 2 parental species S. haematobium and S. intercalatum. Individual worms were identified by electrophoretic analysis of glucose-6-phosphate dehydrogenase, which was characteristic for each isolate. Analysis of the data obtained showed that both heterospecific and homospecific pairs formed between the hybrids and S. haematobium and S. intercalatum. S. haematobium and the hybrid are better than S. intercalatum in forming pairs, and S. haematobium showed a greater homospecific mate preference compared with the hybrid. Analysis of the data using the Mantel-Haenszel test suggests that mating competition does exist between the schistosomes, with the hybrid being dominant over both the parental species and S. haematobium being dominant over S. intercalatum. The hybrid males showed a greater ability than S. intercalatum and S. haematobium males in taking away S. haematobium and S. intercalatum females from their homospecific males when introduced into a pre-established S. haematobium or S. intercalatum infection. They were able to take females from S. intercalatum homospecific pairs more easily compared with females from S. haematobium homospecific pairs. The significance of the results is discussed in relation to the epidemiological changes of schistosomiasis in Cameroon, where hybridization between S. haematobium and S. intercalatum has taken place, with S. haematobium and the hybrid managing to replace the endemic S. intercalatum over the last 30 years.

Description of a new species of Auriculostoma (Digenea: Allocreadiidae) from Characidium heirmostigmata (Characiformes: Crenuchidae) from Argentina, using morphological and molecular data

2021 ◽  
Vol 95 ◽  
Author(s):  
M.M. Montes ◽  
J. Barneche ◽  
Y. Croci ◽  
D. Balcazar ◽  
A. Almirón ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
National Park ◽  
Molecular Data ◽  
Rrna Gene ◽  
28S Rrna ◽  
The Family ◽  
Parasitological Survey ◽  
Morphological And Molecular Data ◽  
A New Species

Abstract During a parasitological survey of fishes at Iguazu National Park, Argentina, specimens belonging to the allocreadiid genus Auriculostoma were collected from the intestine of Characidium heirmostigmata. The erection of the new species is based on a unique combination of morphological traits as well as on phylogenetic analysis. Auriculostoma guacurarii n. sp. resembles four congeneric species – Auriculostoma diagonale, Auriculostoma platense, Auriculostoma tica and Auriculostoma totonacapanensis – in having smooth and oblique testes, but can be distinguished by a combination of several morphological features, hosts association and geographic distribution. Morphologically, the new species can be distinguished from both A. diagonale and A. platense by the egg size (bigger in the first and smaller in the last); from A. tica by a shorter body length, the genital pore position and the extension of the caeca; and from A. totonacapanensis by the size of the oral and ventral sucker and the post-testicular space. Additionally, one specimen of Auriculostoma cf. stenopteri from the characid Charax stenopterus (Characiformes) from La Plata River, Argentina, was sampled and the partial 28S rRNA gene was sequenced. The phylogenetic analysis revealed that A. guacurarii n. sp. clustered with A. tica and these two as sister taxa to A. cf. stenopteri. The new species described herein is the tenth species in the genus and the first one parasitizing a member of the family Crenuchidae.

Three mutations analysis of glucose-6-phosphate dehydrogenase deficiency in neonates in South-west China

2007 ◽  
Vol 49 (4) ◽  
pp. 463-467
Author(s):  
CHUN DENG ◽  
CHUN-BAO GUO ◽  
YOU-HUA XU ◽  
BING DENG ◽  
JIA-LIN YU
Keyword(s):  
Dehydrogenase Deficiency ◽  
West China ◽  
South West ◽  
Glucose 6 Phosphate Dehydrogenase

Comparison of human and murine isolates of Schistosoma mansoni from Richard-Toll, Senegal, by isoelectric focusing

1997 ◽  
Vol 71 (2) ◽  
pp. 175-181 ◽  
Author(s):  
M. Sène ◽  
P. Brémond ◽  
J.P. Hervé ◽  
V.R. Southgate ◽  
B. Sellin ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Acid Phosphatase ◽  
Lactate Dehydrogenase ◽  
Schistosoma Mansoni ◽  
Isoelectric Focusing ◽  
Glucose Phosphate Isomerase ◽  
Glucose Phosphate ◽  
Enzyme Systems ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Arvicanthis Niloticus

AbstractStudies on human and murine isolates of Schistosoma mansoni, from Richard-Toll, Senegal, were carried out by isoelectric focusing in polyacrylamide gels. Seven enzyme systems; lactate dehydrogenase (LDH), malate dehydrogenase (MDH), glucose-6-phosphate dehydrogenase (G6PD), acid phosphatase (AcP), hexokinase (HK), glucose phosphate isomerase (GPI), and phosphoglucomutase (PGM), were used to compare the two isolates. All systems tested, apart from LDH, were found to be polymorphic for both isolates. Interestingly, one phenotype is more frequent than the remainder. The results show that there is no significant genetic variation between the S. mansoni isolates from man and the rodents, Arvicanthis niloticus and Mastomys huberti.

Molecular evidence for the hybrid origin of Rosa lichiangensis (Rosaceae)

Phytotaxa ◽  
2015 ◽  
Vol 222 (3) ◽  
pp. 221 ◽  
Author(s):  
Zhangming Zhu ◽  
Xinfen Gao
Keyword(s):  
Significant Role ◽  
Single Copy ◽  
Morphological Characters ◽  
Molecular Data ◽  
Natural Hybridization ◽  
Hybrid Origin ◽  
Molecular Evidence ◽  
Nuclear Marker

Natural hybridization was assumed to play a significant role in the diversification of Rosa. Rosa lichiangensis was suspected to be of hybrid origin based on its intermediate morphological characters between R. soulieana and R. multiflora var. cathayensis. In this study, four chloroplast regions (ndhC-trnV, ndhF-rpl32, ndhJ-trnF, and psbJ-petA) and a single copy nuclear marker (GAPDH) were used to test the hybrid origin of R. lichiangensis. The results from molecular data supported the hybrid origin of R. lichiangensis and further identified R. soulieana as its maternal progenitor and R. multiflora var. cathayensis as the paternal progenitor.

The interaction of Schistosoma haematobium and S. guineensis in Cameroon

2005 ◽  
Vol 79 (3) ◽  
pp. 193-197 ◽  
Author(s):  
B.L. Webster ◽  
L.A. Tchuem Tchuenté ◽  
J. Jourdane ◽  
V.R. Southgate
Keyword(s):  
Internal Transcribed Spacer ◽  
Schistosoma Haematobium ◽  
Introgressive Hybridization ◽  
Biological Data ◽  
Hybrid Zones ◽  
Hybridization Data ◽  
South West ◽  
Single Stranded Conformational Polymorphism ◽  
Second Internal Transcribed Spacer ◽  
Species Being

AbstractInteractions between schistosomes are complex with some different species being able to mate and hybridize. The epidemiology of schistosomiasis in specific areas of South West Cameroon has evolved remarkably over 30 years as a result of hybridization between Schistosoma guineensis and S. haematobium. Morphological and biological data suggest that S. haematobium replaced S. guineensis in areas of Cameroon through introgressive hybridization. Data are reported on the use of single stranded conformational polymorphism (SSCP) analysis of the nuclear ribosomal second internal transcribed spacer (ITS2) of individual schistosomes from hybrid zones of Cameroon. The data show that since 1990 S. haematobium has completely replaced S. guineensis in Loum, with S. haematobium and the recombinants still present in 2000. This study illustrates the complexities of the dynamics between S. haematobium and S. guineensis in South West Cameroon.

scholarly journals The Indo-Pacific Amalda (Neogastropoda, Olivoidea, Ancillariidae) revisited with molecular data, with special emphasis on New Caledonia

2020 ◽  
Author(s):  
Yuri I. Kantor ◽  
Magalie Castelin ◽  
Alexander Fedosov ◽  
Philippe Bouchet
Keyword(s):  
Gene Flow ◽  
Molecular Analysis ◽  
New Caledonia ◽  
Narrow Range ◽  
Regional Scale ◽  
Molecular Data ◽  
Shell Morphology ◽  
Species Limits ◽  
South West ◽  
Strait Of Malacca

In the ancillariid genus Amalda, the shell is character rich and 96 described species are currently treated as valid. Based on shell morphology, several subspecies have been recognized within Amalda hilgendorfi, with a combined range extending at depths of 150–750 m from Japan to the South-West Pacific. A molecular analysis of 78 specimens from throughout this range shows both a weak geographical structuring and evidence of gene flow at the regional scale. We conclude that recognition of subspecies (richeri Kilburn & Bouchet, 1988, herlaari van Pel, 1989, and vezzaroi Cossignani, 2015) within A. hilgendorfi is not justified. By contrast, hilgendorfi-like specimens from the Mozambique Channel and New Caledonia are molecularly segregated, and so are here described as new, as Amalda miriky sp. nov. and A. cacao sp. nov., respectively. The New Caledonia Amalda montrouzieri complex is shown to include at least three molecularly separable species, including A. allaryi and A. alabaster sp. nov. Molecular data also confirm the validity of the New Caledonia endemics Amalda aureomarginata, A. fuscolingua, A. bellonarum, and A. coriolis. The existence of narrow range endemics suggests that the species limits of Amalda with broad distributions, extending, e.g., from Japan to Taiwan (A. hinomotoensis) or even Indonesia, the Strait of Malacca, Vietnam and the China Sea (A. mamillata) should be taken with caution.

Hybridization in leopard frogs (Rana pipiens complex): terrestrial performance of newly metamorphosed hybrid and parental genotypes in field enclosures

2001 ◽  
Vol 79 (9) ◽  
pp. 1552-1558 ◽  
Author(s):  
Matthew J Parris
Keyword(s):  
Growth Rates ◽  
Life Histories ◽  
Parental Species ◽  
Natural Populations ◽  
Life Cycles ◽  
Natural Hybridization ◽  
Evolutionary Potential ◽  
Leopard Frogs ◽  
Rana Blairi ◽  
Advanced Generation

Terrestrial ecology has been largely neglected in the study of amphibian life histories because it is difficult to manipulate most species during the terrestrial stage. I examined the terrestrial performance of Rana blairi, Rana sphenocephala, and four hybrid (two F1 and two advanced generation) genotypes in replicated experimental enclosures to test for differences in traits related to juvenile terrestrial fitness. I produced all genotypes by means of artificial fertilizations using frogs collected from natural populations in central Missouri, and juvenile frogs were obtained from larvae reared in experimental ponds. Following metamorphosis, froglets were raised in single-genotype groups in terrestrial enclosures through the first overwintering. The proportion surviving did not vary among genotypes, but the power to detect significant differences was low. F1 hybrid genotypes BS and SB demonstrated significantly higher growth rates than either parental species or advanced-generation hybrid genotypes. Observation of growth rates of advanced-generation hybrids equal to those of the parental species, and heterosis in F1 hybrids for growth rate, suggests that natural hybridization between R. blairi and R. sphenocephala can produce novel and relatively fit hybrid genotypes. Direct measurement of multiple fitness components for hybrid and parental genotypes is critical for assessing the evolutionary potential of natural hybridization in organisms with complex life cycles.

scholarly journals G6PD isoenzyme analysis of myeloid and lymphoid cells in human multilineage colonies

Blood ◽  
1984 ◽  
Vol 63 (6) ◽  
pp. 1481-1487 ◽  
Author(s):  
B Lim ◽  
N Jamal ◽  
D Tritchler ◽  
HA Messner
Keyword(s):  
T Cells ◽  
T Cell ◽  
Peripheral Blood ◽  
Myeloid Cells ◽  
Direct Analysis ◽  
Lymphoid Cells ◽  
Isoenzyme Analysis ◽  
Liquid Suspension ◽  
Cell Components ◽  
Glucose 6 Phosphate Dehydrogenase

Abstract Some multilineage hemopoietic colonies contain, in addition to myeloid cells, T lymphocytes. These proliferate extensively in liquid suspension culture under the influence of a T cell growth factor provided by phytohemagglutinin-T cell-conditioned medium (PHA-TCM). The clonal origin of these myeloid and lymphoid components was investigated by determining the glucose-6-phosphate dehydrogenase (G6PD) isoenzyme types of multilineage colonies grown from peripheral blood of 4 G6PD heterozygous normal volunteers. The G6PD assay is sufficiently sensitive to detect enzyme concentrations contributed by as few as 30 granulocytes and erythroblasts, 4–6 megakaryocytes, 2–3 macrophages, and 50–100 T cells. T cell components can be detected even if myeloid cells are present in 10–20-fold excess. A small number of multilineage colonies with T cells produced a single G6PD isoenzyme on direct analysis and after expansion in liquid culture. This observation supports the view of a common progenitor for myeloid and lymphoid cells in the peripheral blood of normal adults.

Volatile oils and the study of natural hybridization between Correa aemula and C. reflexa (Rutaceae)

1977 ◽  
Vol 25 (3) ◽  
pp. 291 ◽  
Author(s):  
T Whiffin
Keyword(s):  
Numerical Analysis ◽  
Parental Species ◽  
Volatile Oil ◽  
Natural Hybridization ◽  
Volatile Oils ◽  
Hybrid Swarm ◽  
Hybrid Plants ◽  
Complex Variation

A small hybrid swarm between Correa aemula and C. reflexa was located and examined morphologically and chemically, with an emphasis on volatile oils. The hybrid plants were intermediate morphologically between the two parental species. The volatile oil data were subjected to multivariate numerical analysis, the results of which confirmed the general intermediacy of the hybrid plants. In general it was found that ordination is a more useful technique for the study of hybridization than is classification. It would appear that numerical analysis of volatile oil data will prove a useful technique in the study of the complex variation found within the genus Correa.

Sign in / Sign up

Export Citation Format

Share Document