Comparison of the seed germination and early seedling growth of soybean in saline conditions

2002 ◽  
Vol 12 (3) ◽  
pp. 165-172 ◽  
Author(s):  
Mohammad Khajeh Hosseini ◽  
Alison A. Powell ◽  
Ian J. Bingham
Keyword(s):  
Growth Rate ◽  
Seedling Growth ◽  
Embryonic Axis ◽  
Lower Sensitivity ◽  
Soybean Seeds ◽  
Early Seedling Growth ◽  
Seedling Growth Rate ◽  
Early Seedling ◽  
Saline Solutions ◽  
Paper Towels

Germination and seedling growth of soybean (Glycine maxL.) cv. Williams were examined on paper towels pre-moistened with a range of saline solutions (germination: 0–500 mMolal NaCl; seedling growth: 0–330 mMolal NaCl). The Na+, K+and Ca2+concentrations in the embryonic axis immediately before germination and in the seedling 3.5 d after germination were measured. Germination decreased at NaCl concentrations of 330 mMolal (81% germination) and above. At 420 mMolal NaCl, only 40% of seeds germinated, and at 500 mMolal NaCl there was no germination. Seedling growth rate decreased drastically with increasing salinity. At 220 mMolal NaCl, seedling growth rate had declined to 5% of the control, whereas at 330 mMolal NaCl seedling growth was almost zero 3–4 d after germination. Thus, soybean seeds were more tolerant of salinity in the germination than in the seedling phase. The results suggest that the greater tolerance of salinity during the germination phase might, in part, be the result of a lower sensitivity to high tissue Na+concentrations. Germination (40%) was possible at a tissue Na+concentration in the embryonic axis of 9.3 mg g FW-1, whereas seedling growth was completely inhibited at a tissue Na+concentration of 6.1 mg g FW-1. Germination at higher tissue Na+concentrations was associated with higher K+and Ca2+concentrations in the embryo axis, compared with growing seedlings, suggesting that these ions may protect the seeds in the pre-germination phase against salinity.

Identification of biotinylated proteins in soybean [Glycine max (L.) Merrill] seeds and their characterization during germination and seedling growth

1997 ◽  
Vol 7 (4) ◽  
pp. 373-376 ◽  
Author(s):  
Robert G. Shatters ◽  
Soon P. Boo ◽  
José B. França Neto ◽  
S. H. West
Keyword(s):  
Seedling Growth ◽  
Biologically Active ◽  
Soybean Seeds ◽  
Soybean Seedling ◽  
Prosthetic Group ◽  
Embryonic Axes ◽  
Plant Parts ◽  
Early Seedling Growth ◽  
Glycine Max L ◽  
Early Seedling

AbstractBiotin is an important vitamin. It is biologically active as a protein prosthetic group, where it functions in enzymatically catalysed carboxylation reactions. It has previously been shown that the ability to synthesize biotin is not necessary for germination of Arabidopsis thaliana seeds, but that this process is required for early seedling growth. This research was conducted to determine if changes in the detection of biotinylated proteins could be observed that reflect changes in the need for biotin-mediated enzyme reactions observed during early soybean seedling growth. A seed specific 75-kDa biotinylated protein present in the embryonic axes and the cotyledons was lost during the first 3 d of germination. Seed specificity, and pattern of expression during germination suggest that this protein is a homologue of the seed specific 65-kDa biotinylated protein previously identified in pea (Pisum sativum). If samples were not treated with 2-mercaptoethanol, three equally spaced proteins at approx. 85 kDa were visible. In the presence of 2-ME these proteins appeared as a single 85-kDa band. This triplet was distinct only in the embryonic axes of dry seeds and not in imbibed seeds or in other plant parts. This demonstrates that imbibitional changes do occur in the pool of biotinylated proteins present in dry soybean seeds, and that 2-ME treatment can inhibit complete identification of the biotinylated proteins present in seed tissues.

scholarly journals Evaluation of Seedling Size Following Germination Using Computer-aided Analysis of Digital Images from a Flat-bed Scanner

HortScience ◽  
2001 ◽  
Vol 36 (6) ◽  
pp. 1117-1120 ◽  
Author(s):  
Robert L. Geneve ◽  
Sharon T. Kester
Keyword(s):  
Growth Rate ◽  
Seedling Growth ◽  
Digital Images ◽  
Petri Dish ◽  
Cellulose Film ◽  
Early Seedling Growth ◽  
Computer Aided Analysis ◽  
Computer Aided ◽  
Early Seedling ◽  
Flat Bed Scanner

Early seedling growth rate can be used to estimate seed vigor for small-seeded vegetable and flower seeds. However, hand measurement of small seedlings is tedious and difficult to reproduce among analysts. Computer-aided analysis digital images of seedlings should improve accuracy and reproducibility. A flat-bed scanner fitted with base and top lighting provided high resolution images of even small-seeded species like petunia [Petunia ×hybrida `Blue Picotee' (Hort) Vilm.] and lisianthus [Eustoma grandiflorum `Mariachi Pure White' (Raf.) Shinn]. Uniform lighting was provided and images were captured and analyzed in less than 2 minutes. A clear, cellulose film was used as the germination substrate in petri dish germination assays to facilitate capturing images with a flat-bed scanner. The transparent medium permitted seedlings to be imaged without removal from the petri dish and also allowed for repeated measures of the same seedlings in order to calculate growth rate. Six species evaluated in this study included cauliflower (Brassica oleracea L., var. Botrytis), tomato (Lycopersicon esculentum Mill. `New Yorker'), pepper (Capsicum annuum L. `North Star'), impatiens [Impatiens walleriana Hook. f. `Impact Lavender'], vinca [Catharanthus roseus (L.) G. Don. `Little Bright Eye'], and marigold (Tagetes patula L. `Little Devil Flame'). For germination and early seedling growth, the cellulose film compared favorably with other standard germination media (blue blotter and germination paper) for five of the six species tested. Computer analysis of seedling length was possible for all six species and was statistically similar to hand measurements averaged for three analysts.

Effect of seed mass on early seedling growth of five eastern white pine (Pinus strobus L.) families under contrasting light environments

2004 ◽  
Vol 82 (11) ◽  
pp. 1645-1655 ◽  
Author(s):  
William C Parker ◽  
Thomas L Noland ◽  
Andrée E Morneault
Keyword(s):  
Growth Rate ◽  
Seedling Growth ◽  
Natural Regeneration ◽  
Seed Mass ◽  
Pinus Strobus ◽  
White Pine ◽  
Eastern White Pine ◽  
Relative Growth ◽  
Early Seedling Growth ◽  
Early Seedling

The strong influence of seed mass on early seedling growth of forest tree species is well established, but the ecological role of intraspecific variation in seed mass on natural regeneration is poorly understood. Wide variation in initial spatial patterns of natural regeneration of eastern white pine (Pinus strobus L.) between managed and undisturbed white pine forests of the Great Lakes region of North America has been attributed to differences in understory light levels and the frequency of substrates suitable for germination and seedling establishment. To explore the potential influence of seed mass on these observed patterns, the interaction of seed mass and light on early growth of white pine was investigated in a greenhouse study. Seedlings of five half-sib families differing in mean seed mass were grown for 8 and 14 weeks under moderate and low light conditions representing managed and undisturbed pine stands, respectively. Family differences in seedling biomass and root system development under each light environment were associated with positive, linear relationships with mean seed mass. Family and seed mass had a comparatively weak influence on biomass partitioning and relative growth rate. Moderate light improved seedling growth regardless of seed mass, but relative expression of seed mass effects on seedling traits was unaffected by light environment. These results suggest differential recruitment of white pine in managed and undisturbed stands is unrelated to variation in seed mass.Key words: biomass allocation, eastern white pine, greenhouse experiment, natural regeneration, relative growth rate, seed mass.

Effects of salinity on seedling emergence and early seedling growth of Irvingia gabonensis (Irvingiaceae)

2017 ◽  
Vol 45 (2) ◽  
pp. 282-295
Author(s):  
R. Vihotogbé ◽  
C. Watson ◽  
R. Glèlè Kakaï ◽  
F. Wichern ◽  
B. Sinsin ◽  
...  
Keyword(s):  
Seedling Growth ◽  
Seedling Emergence ◽  
Early Seedling Growth ◽  
Early Seedling ◽  
Irvingia Gabonensis

scholarly journals The CBL-Interacting Protein Kinase NtCIPK23 Positively Regulates Seed Germination and Early Seedling Development in Tobacco (Nicotiana tabacum L.)

Plants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 323
Author(s):  
Sujuan Shi ◽  
Lulu An ◽  
Jingjing Mao ◽  
Oluwaseun Olayemi Aluko ◽  
Zia Ullah ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Seed Germination ◽  
Seedling Growth ◽  
Hypocotyl Elongation ◽  
Interacting Protein ◽  
Knock Out ◽  
Early Seedling Growth ◽  
Tobacco Seedlings ◽  
Early Seedling ◽  
Cotyledon Expansion

CBL-interacting protein kinase (CIPK) family is a unique group of serine/threonine protein kinase family identified in plants. Among this family, AtCIPK23 and its homologs in some plants are taken as a notable group for their importance in ions transport and stress responses. However, there are limited reports on their roles in seedling growth and development, especially in Solanaceae plants. In this study, NtCIPK23, a homolog of AtCIPK23 was cloned from Nicotiana tabacum. Expression analysis showed that NtCIPK23 is mainly expressed in the radicle, hypocotyl, and cotyledons of young tobacco seedlings. The transcriptional level of NtCIPK23 changes rapidly and spatiotemporally during seed germination and early seedling growth. To study the biological function of NtCIPK23 at these stages, the overexpressing and CRISPR/Cas9-mediated knock-out (ntcipk23) tobacco lines were generated. Phenotype analysis indicated that knock-out of NtCIPK23 significantly delays seed germination and the appearance of green cotyledon of young tobacco seedling. Overexpression of NtCIPK23 promotes cotyledon expansion and hypocotyl elongation of young tobacco seedlings. The expression of NtCIPK23 in hypocotyl is strongly upregulated by darkness and inhibited under light, suggesting that a regulatory mechanism of light might underlie. Consistently, a more obvious difference in hypocotyl length among different tobacco materials was observed in the dark, compared to that under the light, indicating that the upregulation of NtCIPK23 contributes greatly to the hypocotyl elongation. Taken together, NtCIPK23 not only enhances tobacco seed germination, but also accelerate early seedling growth by promoting cotyledon greening rate, cotyledon expansion and hypocotyl elongation of young tobacco seedlings.

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