INTRODUCTION: Juvenile idiopathic arthritis (JIA) is the most common rheumatic disease in children characterized by autoimmune etiology. In previous studies, increased Celiac disease (CD) frequency was reported in patients diagnosed with JIA. In this study, CD and non-celiac gluten sensitivity (NCGS) in patients diagnosed with JIA were investigated. METHODS: Sixty-one (57.3%) JIA patients admitted to the pediatric rheumatology outpatient clinic between January 2020 and April 2020 were included in this cross-sectional study. All patients were evaluated with clinical and laboratory findings in terms of CD and NCGS. Total immunoglobulin (Ig)-A, tissue transglutaminase antibody (tTG) IgA and IgG, anti-endomysium-antibody (EMA) IgA and IgG and anti-gliadin-antibody (AGA) IgA and IgG levels were measured in all patients. RESULTS: Sixty-one JIA patients, 35 girls, were included in the study. The mean age of the patients was 11.4 ± 4.6 years, the mean age at diagnosis is 10.2 ± 3.4 years. Thirty-three patients were diagnosed with oligoarticular JIA; 18 patients with enthesitis-related arthritis, 8 patients with polyarticular JIA, and 2 patients with psoriatic arthritis. All patients were using disease-modifying antirheumatic drugs during the study. Thirty-five patients were receiving biological therapy, concomitantly. Two patients had abdominal pain, two patients had indigestion, and two patients had constipation. None of the patients had growth retardation. EMA IgA and IgG, tTG IgA and IgG, AGA IgA and IgG tests were negative in all patients. DISCUSSION AND CONCLUSION: CD and NCGS were not detected in our JIA patients. Multi-center studies may guide clinicians in under what circumstances to perform CD and NCGS screening in JIA patients.