Biomedicine: electrospun nanofibrous hormonal therapies through skin/tissue—a review

1967 ◽

Vol 25 ◽

pp. 180-181

Author(s):

John H.L. Watson ◽

John L. Swedo ◽

R.W. Talley

Keyword(s):

Mammary Carcinoma ◽

Osmium Tetroxide ◽

Estrogen Therapy ◽

Ultrastructural Level ◽

Uranyl Acetate ◽

Human Mammary Carcinoma ◽

Surgical Biopsy ◽

Lead Hydroxide ◽

Preliminary Study ◽

Hormonal Therapies

A preliminary study of human mammary carcinoma on the ultrastructural level is reported for a metastatic, subcutaneous nodule, obtained as a surgical biopsy. The patient's tumor had responded favorably to a series of hormonal therapies, including androgens, estrogens, progestins, and corticoids for recurring nodules over eight years. The pertinent nodule was removed from the region of the gluteal maximus, two weeks following stilbestrol therapy. It was about 1.5 cms in diameter, and was located within the dermis. Pieces from it were fixed immediately in cold fixatives: phosphate buffered osmium tetroxide, glutaraldehyde, and paraformaldehyde. Embedment in each case was in Vestopal W. Contrasting was done with combinations of uranyl acetate and lead hydroxide.

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Duffy antigen / receptor for chemokines correlates with inflammatory reaction in rats with venous hypertension: implication for the pathogenesis of primary chronic venous disease

VASA ◽

10.1024/0301-1526/a000327 ◽

2014 ◽

Vol 43 (1) ◽

pp. 47-54 ◽

Cited By ~ 1

Author(s):

Weibin Huang ◽

Weiwei Qin ◽

Lei Lv ◽

Haoyv Deng ◽

Hao Zhang ◽

...

Keyword(s):

Mononuclear Cells ◽

Early Stage ◽

Antigen Receptor ◽

Venous Hypertension ◽

Skin Tissue ◽

Chronic Venous Disease ◽

Venous Disease ◽

Dependent Manner ◽

Time Points ◽

Duffy Antigen

Background: Duffy antigen / receptor for chemokines (DARC) possesses high affinity for several chemokine subgroups of CC and CXC. Although DARC has been shown to play a role in many inflammatory diseases, its effect on chronic venous disease (CVD) remains unidentified. We explored whether the expression of DARC in skin tissue was activated under venous hypertension as well as the relationships between DARC and inflammation. Materials and methods: The inflammation in a rat model of venous hypertension caused by a femoral arterial-venous fistula (AVF) was studied. At specified intervals the pressure in the femoral veins was recorded within 42 days. Hindlimb skin specimens were harvested at different time points. The expressions of DARC, interleukin-8 (IL-8), and monocyte chemotactic protein-1 (MCP-1) in skin tissue were examined. Mononuclear cells infiltrated in skin tissue were detected. Results: Femoral venous pressures in AVF groups increased significantly at different time points (P < 0.01). DARC was expressed in skin tissue and its expression level increased significantly in AVF groups from the 7nd day on and was enhanced in a time-dependent manner within 42 days (P < 0.05). Meanwhile, both MCP-1 and IL-8 had higher levels, accompanied by increased mononuclear cells infiltrating into skin tissue (P < 0.05). Conclusions: A rat AVF model which can maintain venous hypertension for at least 42 days is competent for researching the pathogenesis of CVD. DARC, which plays a role in the inflammation of skin tissue under venous hypertension, may become a new molecular target for diagnosis and treatment of CVD at a very early stage.

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Numerical analysis of temperature field in domain of skin tissue for different external and internal thermal conditions

Physiotherapy ◽

10.2478/v10109-009-0014-y ◽

2008 ◽

Vol 16 (2) ◽

Author(s):

Bohdan Mochnacki ◽

Marek Jasiński

Keyword(s):

Temperature Field ◽

Numerical Analysis ◽

Thermal Conditions ◽

Skin Tissue

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Needleless electrospinning of PVP/PGS fibrous scaffolds for skin tissue engineering applications

10.31224/osf.io/xs64k ◽

2018 ◽

Cited By ~ 1

Author(s):

Antonios Keirouz ◽

Giuseppino Fortunato ◽

Anthony Callanan ◽

Norbert Radacsi

Keyword(s):

Tissue Engineering ◽

Tensile Modulus ◽

Dermal Fibroblasts ◽

Skin Tissue ◽

Skin Substitute ◽

Electrospinning Technique ◽

Skin Tissue Engineering ◽

Needleless Electrospinning ◽

High Concentrations

Scaffolds and implants used for tissue engineering need to be adapted for their mechanical properties with respect to their environment within the human body. Therefore, a novel composite for skin tissue engineering is presented by use of blends of Poly(vinylpyrrolidone) (PVP) and Poly(glycerol sebacate) (PGS) were fabricated via the needleless electrospinning technique. The formed PGS/PVP blends were morphologically, thermochemically and mechanically characterized. The morphology of the developed fibers related to the concentration of PGS, with high concentrations of PGS merging the fibers together plasticizing the scaffold. The tensile modulus appeared to be affected by the concentration of PGS within the blends, with an apparent decrease in the elastic modulus of the electrospun mats and an exponential increase of the elongation at break. Ultraviolet (UV) crosslinking of PGS/PVP significantly decreased and stabilized the wettability of the formed fiber mats, as indicated by contact angle measurements. In vitro examination showed good viability and proliferation of human dermal fibroblasts over the period of a week. The present findings provide important insights for tuning the elastic properties of electrospun material by incorporating this unique elastomer, as a promising future candidate for skin substitute constructs.

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The exact analytical solution of the dual-phase-lag two-temperature bioheat transfer of a skin tissue subjected to constant heat flux

Scientific Reports ◽

10.1038/s41598-020-73086-0 ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Hamdy M. Youssef ◽

Najat A. Alghamdi

Keyword(s):

Heat Flux ◽

Analytical Solution ◽

Exact Analytical Solution ◽

Constant Heat Flux ◽

Bioheat Transfer ◽

Skin Tissue ◽

Phase Lag ◽

Dual Phase ◽

Constant Heat ◽

Two Temperature

Abstract This work is dealing with the temperature reaction and response of skin tissue due to constant surface heat flux. The exact analytical solution has been obtained for the two-temperature dual-phase-lag (TTDPL) of bioheat transfer. We assumed that the skin tissue is subjected to a constant heat flux on the bounding plane of the skin surface. The separation of variables for the governing equations as a finite domain is employed. The transition temperature responses have been obtained and discussed. The results represent that the dual-phase-lag time parameter, heat flux value, and two-temperature parameter have significant effects on the dynamical and conductive temperature increment of the skin tissue. The Two-temperature dual-phase-lag (TTDPL) bioheat transfer model is a successful model to describe the behavior of the thermal wave through the skin tissue.

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Size-Tunable Paclitaxel Nanoparticles Stabilized by Anionic Phospholipids for Transdermal Delivery Applications

Natural Product Communications ◽

10.1177/1934578x19900684 ◽

2020 ◽

Vol 15 (3) ◽

pp. 1934578X1990068

Author(s):

Noriyuki Uchida ◽

Masayoshi Yanagi ◽

Hiroki Hamada

Keyword(s):

Stratum Corneum ◽

Transdermal Delivery ◽

Skin Tissue ◽

Composite Nanoparticles ◽

Rat Skin ◽

Cooling Process ◽

Anionic Phospholipid ◽

Anionic Phospholipids ◽

Subsequent Heating

Composite nanoparticles composed of an anionic phospholipid of 1,2-dipalmitoyl-sn-glycero-3-phosphorylglycerol (DPPG) and paclitaxel (PTX) were successfully prepared by mixing them in water followed by a subsequent heating/cooling process. The size of DPPG-PTX nanoparticle could be easily tuned by ultrasonic fragmentation. Upon addition of small-sized fluorescently labeled paclitaxel (FLPTX) nanoparticles with DPPG (DPPG-FLPTX) to rat skin tissue, part of the FLPTX molecules permeated to the stratum corneum.

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