A- and B-subgenome characterisation of sucrose synthase family proteins and functional identification of MaSUS2.2 reveals its involvement in the starch accumulation in banana fruit

A transcriptome-wide discovery and functional identification of a sucrose synthase family was presented. Importantly, OcSus1 and OcSus2 were first verified to be responsible for the biosynthesis of glucose-containing polysaccharides inO. caudatum.

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Sucrose Synthase Enhances Hull Size and Grain Weight by Regulating Cell Division and Starch Accumulation in Transgenic Rice

International Journal of Molecular Sciences ◽

10.3390/ijms20204971 ◽

2019 ◽

Vol 20 (20) ◽

pp. 4971 ◽

Cited By ~ 3

Author(s):

Chunfen Fan ◽

Guangya Wang ◽

Youmei Wang ◽

Ran Zhang ◽

Yanting Wang ◽

...

Keyword(s):

Cell Division ◽

Grain Yield ◽

Transgenic Rice ◽

Sucrose Synthase ◽

Grain Weight ◽

Primary Cell Wall ◽

Starch Accumulation ◽

Wild Type ◽

Empty Vector ◽

Transgenic Lines

Grain size and weight are two important determinants of grain yield in rice. Although overexpression of sucrose synthase (SUS) genes has led to several improvements on cellulose and starch-based traits in transgenic crops, little is reported about SUS enhancement of hull size and grain weight in rice. In this study, we selected transgenic rice plants that overexpressed OsSUS1-6 genes driven with the maize Ubi promoter. Compared to the controls (wild type and empty vector line), all independent OsSUS homozygous transgenic lines exhibited considerably increased grain yield and grain weights. Using the representative OsSUS3 overexpressed transgenic plants, four independent homozygous lines showed much raised cell numbers for larger hull sizes, consistent with their enhanced primary cell wall cellulose biosynthesis and postponed secondary wall synthesis. Accordingly, the OsSUS3 transgenic lines contained much larger endosperm volume and higher starch levels than those of the controls in the mature grains, leading to increased brown grain weights by 15–19%. Hence, the results have demonstrated that OsSUS overexpression could significantly improve hull size and grain weight by dynamically regulating cell division and starch accumulation in the transgenic rice.

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BT1, a protein critical for in vivo starch accumulation in maize endosperm, is not detected in maize endosperm suspension cultures

Physiologia Plantarum ◽

10.1034/j.1399-3054.1996.970406.x ◽

1996 ◽

Vol 97 (4) ◽

pp. 665-673 ◽

Cited By ~ 1

Author(s):

Heping Cao ◽

Jack C. Shannon

Keyword(s):

Suspension Cultures ◽

Starch Accumulation ◽

Maize Endosperm

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Klasifikasi Tahap Kematangan Pisang Ambon Berdasarkan Warna Menggunakan Naive Bayes

PIKSEL : Penelitian Ilmu Komputer Sistem Embedded and Logic ◽

10.33558/piksel.v5i2.268 ◽

2018 ◽

Vol 5 (2) ◽

pp. 60-67 ◽

Cited By ~ 1

Author(s):

Dwi Yulianto ◽

Retno Nugroho Whidhiasih ◽

Maimunah Maimunah

Keyword(s):

Naive Bayes ◽

Fruit Production ◽

Naïve Bayes ◽

Primary Data ◽

Banana Fruit ◽

Bayes Method ◽

Classification Image ◽

Average Accuracy ◽

The Government

ABSTRACT Banana fruit is a commodity that contributes a great value to both national and international fruit production achievement. The government through the National Standardization Agency establishes standards to maintain the quality of bananas. The purpose of this Project is to classify the stages of maturity of Ambon banana base on the color index using Naïve Bayes method in accordance with the regulations of SNI 7422:2009. Naive Bayes is used as a method in the classification process by comparing the probability values generated from the variable value of each model to determine the stage of Ambon banana maturity. The data used is the primary data image of 105 pieces of Ambon banana. By using 3 models which consists of different variables obtained the same greatest average accuracy by using the 2nd model which has 9 variable values (r, g, b, v, * a, * b, entropy, energy, and homogeneity) and the 3rd model has 7 variable values (r, g, b, v , * a, entropy and homogeneity) that is 90.48%. Keywords: banana maturity, classification, image processing ABSTRAK Buah pisang merupakan komoditas yang memberikan kontribusi besar terhadap angka produksi buah nasional maupun internasional. Pemerintah melalui Badan Standarisasi Nasional menetapkan standar untuk buah pisang, menjaga mutu buah pisang. Tujuan dari penelitian ini adalah klasifikasi tahapan kematangan dari buah pisang ambon berdasarkan indeks warna menggunakan metode Naïve Bayes sesuai dengan SNI 7422:2009. Naive bayes digunakan sebagai metode dalam proses pengklasifikasian dengan cara membandingkan nilai probabilitas yang dihasilkan dari nilai variabel penduga setiap model untuk menentukan tahap kematangan pisang ambon. Data yang digunakan adalah data primer citra pisang ambon sebanyak 105. Dengan menggunakan 3 buah model yang terdiri dari variabel penduga yang berbeda didapatkan akurasi rata-rata terbesar yang sama yaitu dengan menggunakan model ke-2 yang mempunyai 9 nilai variabel (r, g, b, v, *a, *b, entropi, energi, dan homogenitas) dan model ke-3 yang mempunyai 7 nilai variabel (r, g, b, v, *a, entropi dan homogenitas) yaitu sebesar 90.48%. Kata Kunci : kematangan pisang, klasifikasi, pengolahan citra

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Co-expression of AGPS and AGPL genes encoded for AGPase from cassava to enhance starch accumulation in transgenic tobacco

Vietnam Journal of Biotechnology ◽

10.15625/1811-4989/14/2/9354 ◽

2016 ◽

Vol 14 (2) ◽

pp. 287-293

Author(s):

Nguyễn Văn Đoài ◽

Nguyễn Minh Hồng ◽

Lê Thu Ngọc ◽

Nguyễn Thị Thơm ◽

Nguyễn Đình Trọng ◽

...

Keyword(s):

Transgenic Tobacco ◽

Higher Plants ◽

Starch Content ◽

Genetically Modified Crops ◽

Starch Accumulation ◽

35S Promoter ◽

Effective Strategies ◽

Plant Expression Vector ◽

Transgenic Lines ◽

Cassava Varieties

The AGPase (ADP-Glucose pyrophosphorylase) is one of the ubiquitous enzymes catalyzing the first step in starch biosynthesis. It plays an important role in regulation and adjusts the speed of the entire cycle of glycogen biosynthesis in bacteria and starch in plants. In higher plants, it is a heterotetramer and tetrameric enzyme consisting two large subunits (AGPL) and two small subunits (AGPS) and encoded by two genes. In this paper, both AGPS and AGPL genes were sucessfully isolated from cassava varieties KM140 and deposited in Genbank with accession numbers KU243124 (AGPS) and KU243122 (AGPL), these two genes were fused with P2a and inserted into plant expression vector pBI121 under the control of 35S promoter. The efficient of this construct was tested in transgenic N. tabacum. The presence and expression of AGPS and AGPL in transgenic plants were confirmed by PCR and Western hybridization. The starch content was quantified by the Anthrone method. Transgenic plant analysis indicated that that two targeted genes were expressed simultaneously in several transgenic tobacco lines under the control of CaMV 35S promoter. The starch contents in 4 analyzed tobacco transgenic lines displays the increase 13-116% compared to WT plants. These results indicated that the co-expression of AGPS and AGPL is one of effective strategies for enhanced starch production in plant. These results can provide a foundation for developing other genetically modified crops to increase starch accumulation capacity.

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