Comparison of two SH-based methods for estimation of metallothionein level in the digestive gland of naturally occurring mussels,Mytilus galloprovincialis

2003 ◽  
Vol 83 (3) ◽  
pp. 219-231 ◽  
Author(s):  
DuŠica IvankoviĆ ◽  
Jasenka PaviČiĆ ◽  
Biserka Raspor ◽  
Ingrid Falnoga ◽  
Magda TuŠek-ŽnidariČ
Keyword(s):  
Digestive Gland ◽  
Mytilus Galloprovincialis ◽  
Naturally Occurring

scholarly journals Characterization of the Domoic Acid Uptake Mechanism of the Mussel (Mytilus galloprovincialis) Digestive Gland

Toxins ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 458
Author(s):  
Juan Blanco ◽  
Carmen Mariño ◽  
Helena Martín ◽  
Gonzalo Álvarez ◽  
Araceli E. Rossignoli
Keyword(s):  
Digestive Gland ◽  
Domoic Acid ◽  
Mytilus Galloprovincialis ◽  
Metabolic Inhibitor ◽  
Acid Uptake ◽  
Free Medium ◽  
Shellfish Poisoning ◽  
Uptake Mechanism ◽  
Amnesic Shellfish Poisoning

Cultures of the mussel Mytilus galloprovincialis are frequently affected by accumulation of the amnesic shellfish poisoning toxin domoic acid (DA). This species is characterized by a fast uptake and release of the toxin. In this work, the main characteristics of the uptake mechanism have been studied by incubation of digestive gland thin slices in media with different composition and DA concentration. DA uptake seems to follow Michaelis–Menten kinetics, with a very high estimated KM (1722 µg DA mL−1) and a Vmax of 71.9 µg DA g−1 h−1, which is similar to those found for other amino acids in invertebrates. Replacement of NaCl from the incubation media by Cl-choline (Na+-free medium) did not significantly reduce the uptake, but replacement by sorbitol (Na+-free and Cl−-depleted medium) did. A new experiment replacing all chlorides with their equivalent gluconates (Na+- and Cl−-free medium) showed an important reduction in the uptake that should be attributed to the absence of chloride, pointing to a Na+-independent, Cl− (or anion-) dependent transporter. In media with Na+ and Cl−, neither decreasing the pH nor adding cyanide (a metabolic inhibitor) had significant effect on DA uptake, suggesting that the transport mechanism is not H+- or ATP-dependent. In a chloride depleted medium, lowering pH or adding CN increased the uptake, suggesting that other anions could, at least partially, substitute chloride.

Antioxidant enzyme activities and lipid peroxidation in Mytilus galloprovincialis from the French Mediterranean coast

2010 ◽  
Vol 39 (4) ◽  
Author(s):  
Krzysztof Gwoździński ◽  
Marta Gonciarz ◽  
Ewa Kilańczyk ◽  
Aleksandra Kowalczyk ◽  
Anna Pieniążek ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Oxidative Damage ◽  
Antioxidant Enzyme ◽  
Digestive Gland ◽  
Enzyme Activities ◽  
Mytilus Galloprovincialis ◽  
Mediterranean Coast ◽  
Antioxidant Enzyme Activities ◽  
Gland Tissue ◽  
Two Populations

Antioxidant enzyme activities and lipid peroxidation inIn the present work we have studied some of the indicators of oxidative damage of the digestive gland tissue of two populations of mussels

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