Exploring the nematicidal activity of plant extracts for management of Meloidogyne incognita in local cultivars of eggplant (Solanum melongena L.) in Pakistan

Abstract Background The root-knot nematode, Meloidogyne incognita, causes a high damage and yield decrease for many economic plants. The need for non-systemic effective new approaches and environmentally friendly methods for controlling the nematodes has directed research to some new and safe agrochemicals found in medicinal plants as new viable management options. Results In laboratory experiments, solidago and periwinkle aqueous and ethanolic extracts achieved high J2 mortality (%) concerning different dilutions; however, aqueous extracts were more effective for mortality than ethanolic extracts. Also, there was a direct relationship between the nematicidal activity of these extracts with both concentration and time of application. Inhibition of egg hatching by Periwinkle extracts was higher than that of solidago. Moreover, the nematicidal activity of tested extracts against J2 decreased significantly with prolonged storage time at + 5 °C, while did not with stored frozen at – 5 °C for 12 months. Periwinkle and solidago extracts killed the non-target organisms, i.e., rotifers and free-living nematodes. Seventy-five and 90% of total phytochemicals recovered from periwinkle and solidago, respectively were nematostatic or nematicidal to nematode viability, egg hatch in vitro, and development and reproduction in vivo despite the method of application (foliar and soil drench). The antagonistic effects of solidago were more pronounced in soil drench than periwinkle concerning their concentrations and methods of application. Conclusion Solidago and periwinkle plant extracts showed important sources of effective control phytochemicals against M. incognita.

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Nematicidal activity of leaf extracts from Lantana camara L. against Meloidogyne incognita (kofoid and white) chitwood and its use to manage roots infection of Solanum melongena L.

Brazilian Archives of Biology and Technology ◽

10.1590/s1516-89132010000300006 ◽

2010 ◽

Vol 53 (3) ◽

pp. 543-548 ◽

Cited By ~ 10

Author(s):

Faheem Ahmad ◽

Mohmad Azhar Rather ◽

Mansoor Ahmad Siddiqui

Keyword(s):

Meloidogyne Incognita ◽

Solanum Melongena ◽

Lantana Camara ◽

Nematicidal Activity ◽

Leaf Extracts

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Nematicidal Activity of Plant Extracts Against the Root-Knot Nematode, Meloidogyne incognita

The Open Natural Products Journal ◽

10.2174/1874848100902010077 ◽

2009 ◽

Vol 2 (1) ◽

pp. 77-85 ◽

Cited By ~ 30

Author(s):

Wiratno ◽

D. Taniwiryono ◽

H. Van den Berg ◽

J.A. G. Riksen ◽

I.M. C.M. Rietjens ◽

...

Keyword(s):

Plant Extracts ◽

Meloidogyne Incognita ◽

Nematicidal Activity ◽

Root Knot Nematode

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Application of Bacillus species for controlling root-knot nematode Meloidogyne incognita in eggplant

Bulletin of the National Research Centre ◽

10.1186/s42269-019-0187-6 ◽

2019 ◽

Vol 43 (1) ◽

Author(s):

Wafaa M. A. El-Nagdi ◽

Hassan Abd-El-Khair

Keyword(s):

Meloidogyne Incognita ◽

Growth Parameters ◽

Solanum Melongena ◽

Nematicidal Activity ◽

Biocontrol Agents ◽

Bacillus Species ◽

Vegetable Crops ◽

Root Knot Nematode ◽

Egg Masses ◽

Applied Dose

Abstract Background Eggplant (Solanum melongena L.) is one of the important vegetable crops infected by Meloidogyne incognita all over the world, including Egypt. Chemical nematicides frequently cause environmental pollution and toxic hazards to human, plants, and animals; certain biocontrol agents that are environmentally friendly and safe to humans and animals were tested against the root-knot nematode Meloidogyne incognita in eggplant. Objective This work is aimed to determine the nematicidal activity of Bacillus spp., viz., B. subtilis and B. pumilus, against M. incognita in three separated experiments to study their ability in controlling M. incognita and in improving the growth parameters of eggplants. Bacillus spp. were applied as single or in combination (experiment I), single treatment at different doses (experiment II), and different times (three times) of application (experiment III). Results The results of experiment I revealed that Bacillus sp. + B. subtilis significantly reduced the second-stage juvenile (J2) in soil and galls and egg masses in roots, while Bacillus sp. + B. pumilus significantly reduced J2 in roots. Bacillus spp. in pairs were more effective against M. incognita. In experiment II, Bacillus sp. (40 ml) significantly reduced the J2 in soil and galls and egg masses in roots, while B. pumilus (40 ml) significantly reduced the J2 in roots. The nematicidal activity of Bacillus spp. was increased by increasing the applied dose. In experiment III, B. subtilis, when applied three times, significantly reduced the J2 in soil and the J2 and galls in roots, while B. pumilus (applied three times) significantly reduced the egg masses in roots. All Bacillus spp. treatments highly increased the tested growth parameters compared to the controls. Conclusions The tested biocontrol agents used more than once or in combination are more effective than those used only once in controlling nematode parameters in eggplant under greenhouse conditions with a consequent increase in eggplant growth. These bacterial isolates need to be studied under different field conditions for confirmation.

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Bio-potential of Aqueous Extract of Verbesina encelioides, Moringa oleifera, Cassia fistula against Meloidogyne incognita

Indian Journal of Agricultural Research ◽

10.18805/ijare.a-5836 ◽

2021 ◽

Author(s):

Pareek Apexa ◽

Gaur Arvind ◽

Lodha Payal

Keyword(s):

Mortality Rate ◽

Plant Extracts ◽

Meloidogyne Incognita ◽

Plant Material ◽

Moringa Oleifera ◽

Nematicidal Activity ◽

Aqueous Extracts ◽

Human Beings ◽

Cassia Fistula ◽

Verbesina Encelioides

Background: The Meloidogyne incognita is the most destructive pathogen and causes loss of yield of various economically important plants of various families. It is polyphagous in behaviour and has a high reproductive rate, these parameters are a hurdle in management of it. Various chemicals are used to control nematode worldwide that are very expensive and highly toxic to the environment and human beings. For ecologically safe method, three plants i.e. Verbesina encelioides, Moringa oleifera, Cassia fistula and their various parts such as leaf, stem and flower’s cold aqueous extracts were tried in vitro to test their nematicidal activity. Methods: Cold aqueous extracts (10% w/v) of each plant material were prepared by mixing 10g of each plant material in 100 ml of distilled water and kept aside for 48 hours. To estimate inhibition of egg hatching and mortality rate of second stage juveniles of Meloidogyne incognita, eggs and juveniles were exposed for 24, 48 and 72 hours in different concentrations (20 ppm to 200 ppm) of plant extracts. Result: All three tested plant extracts showed promising nematicidal activity against Meloidogyne incognita and the leaf extract of Moringa oleifera prevents the hatching of eggs. Inhibition of larval hatching from egg and nematode mortality rate were strongly influenced by concentration of extract, plant species and duration of exposure.

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