Biodegradation of malathion, α- and β-endosulfan by bacterial strains isolated from agricultural soil in Veracruz, Mexico

The bacterial strains IMB-1T and CC495T, which are capable of growth on methyl chloride (CH3Cl, chloromethane) and methyl bromide (CH3Br, bromomethane), were isolated from agricultural soil in California fumigated with CH3Br, and woodland soil in Northern Ireland, respectively. Two pesticide-/herbicide-degrading bacteria, strains ER2 and C147, were isolated from agricultural soil in Canada. Strain ER2 degrades N-methyl carbamate insecticides, and strain C147 degrades triazine herbicides widely used in agriculture. On the basis of their morphological, physiological and genotypic characteristics, these four strains are considered to represent two novel species of the genus Aminobacter, for which the names Aminobacter ciceronei sp. nov. (type strain IMB-1T=ATCC 202197T=CIP 108660T=CCUG 50580T; strains ER2 and C147) and Aminobacter lissarensis sp. nov. (type strain CC495T=NCIMB 13798T=CIP 108661T=CCUG 50579T) are proposed.

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Combining Culture-Dependent and Independent Approaches for the Optimization of Epoxiconazole and Fludioxonil-Degrading Bacterial Consortia

Microorganisms ◽

10.3390/microorganisms9102109 ◽

2021 ◽

Vol 9 (10) ◽

pp. 2109

Author(s):

Diogo Alexandrino ◽

Ana Mucha ◽

Maria Paola Tomasino ◽

C. Marisa R. Almeida ◽

Maria Carvalho

Keyword(s):

Agricultural Soil ◽

Enrichment Culture ◽

Bacterial Species ◽

The Other ◽

Microbial Consortia ◽

Bacterial Isolates ◽

Bacterial Strains ◽

Bacterial Consortia ◽

First Time ◽

Culture Dependent

Epoxiconazole (EPO) and fludioxonil (FLU) are two widely used fluorinated pesticides known to be highly persistent and with high ecotoxicological potential, turning them into pollutants of concern. This work aimed to optimize two degrading bacterial consortia, previously obtained from an agricultural soil through enrichment with EPO and FLU, by characterizing the contribution of their corresponding bacterial isolates to the biodegradation of these pesticides using both culture-dependent and independent methodologies. Results showed that a co-culture of the strains Hydrogenophaga eletricum 5AE and Methylobacillus sp. 8AE was the most efficient in biodegrading EPO, being able to defluorinate ca. 80% of this pesticide in 28 days. This catabolic performance is likely the result of a commensalistic cooperation, in which H. eletricum may be the defluorinating strain and Methylobacillus sp. may assume an accessory, yet pivotal, catabolic role. Furthermore, 16S rRNA metabarcoding analysis revealed that these strains represent a minority in their original consortium, showing that the biodegradation of EPO can be driven by less abundant phylotypes in the community. On the other hand, none of the tested combinations of bacterial strains showed potential to biodegrade FLU, indicating that the key degrading strains were not successfully isolated from the original enrichment culture. Overall, this work shows, for the first time, the direct involvement of two bacterial species, namely H. eletricum and Methylobacillus sp., in the biodegradation of EPO, while also offering insight on how they might cooperate to accomplish this process. Moreover, the importance of adequate culture-dependent approaches in the engineering of microbial consortia for bioremediation purposes is also emphasized.

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Effect of CuO Nanoparticles over Isolated Bacterial Strains from Agricultural Soil

Journal of Nanomaterials ◽

10.1155/2014/148743 ◽

2014 ◽

Vol 2014 ◽

pp. 1-13 ◽

Cited By ~ 22

Author(s):

Sandra I. Concha-Guerrero ◽

Elcia Margareth Souza Brito ◽

Hilda A. Piñón-Castillo ◽

S. H. Tarango-Rivero ◽

César A. Caretta ◽

...

Keyword(s):

Agricultural Soil ◽

Spherical Shape ◽

Chemical Precipitation ◽

Copper Oxide Nanoparticles ◽

Cuo Nanoparticles ◽

Oxide Reduction ◽

Bacterial Strains ◽

Membrane Degradation ◽

Pantoea Ananatis ◽

Brevibacillus Laterosporus

The increased use of the nanoparticles (NPs) on several processes is notorious. In contrast the ecotoxicological effects of NPs have been scarcely studied. The main current researches are related to the oxide metallic NPs. In the present work, fifty-six bacterial strains were isolated from soil, comprising 17 different OTUs distributed into 3 classes: Bacilli (36 strains), Flavobacteria (2 strains), and Gammaproteobacteria (18 strains). Copper oxide nanoparticles (CuONPs) were synthesized using a process of chemical precipitation. The obtained CuONPs have a spherical shape and primary size less than 17 nm. Twenty-one strains were used to evaluate the cytotoxicity of CuONPs and 11 of these strains showed high sensibility. Among those 11 strains, 4 (Brevibacillus laterosporusstrain CSS8,Chryseobacterium indoltheticumstrain CSA28, andPantoea ananatisstrains CSA34 and CSA35) were selected to determine the kind of damage produced. The CuONPs toxic effect was observed at expositions over 25 mg·L−1and the damage to cell membrane above 160 mg·L−1. The electron microscopy showed the formation of cavities, holes, membrane degradation, blebs, cellular collapse, and lysis. These toxic effects may probably be due to the ions interaction, the oxide-reduction reactions, and the generation of reactive species.

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Cupriavidus pampae sp. nov., a novel herbicide-degrading bacterium isolated from agricultural soil

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ◽

10.1099/ijs.0.018341-0 ◽

2010 ◽

Vol 60 (11) ◽

pp. 2606-2612 ◽

Cited By ~ 35

Author(s):

Virginia Cuadrado ◽

Margarita Gomila ◽

Luciano Merini ◽

Ana M. Giulietti ◽

Edward R. B. Moore

Keyword(s):

Agricultural Soil ◽

Bacterial Consortium ◽

Rrna Gene ◽

Bacterial Strains ◽

Systematic Analysis ◽

Hybridization Data ◽

Degrading Bacterium ◽

History Of ◽

Pampa Region ◽

Herbicide Use

A bacterial consortium able to degrade the herbicide 4-(2,4-dichlorophenoxy) butyric acid (2,4-DB) was obtained from an agricultural soil of the Argentinean Humid Pampa region which has a history of long-term herbicide use. Four bacterial strains were isolated from the consortium and identified as members of the genera Cupriavidus, Labrys and Pseudomonas. A polyphasic systematic analysis was carried out on strain CPDB6T, the member of the 2,4-DB-degrading consortium able to degrade 2,4-DB as a sole carbon and energy source. The Gram-negative, rod-shaped, motile, non-sporulating, non-fermenting bacterium was shown to belong to the genus Cupriavidus on the basis of 16S rRNA gene sequence analyses. Strain CPDB6T did not reduce nitrate, which differentiated it from the type species of the genus, Cupriavidus necator; it did not grow in 0.5–4.5 % NaCl, although most species of Cupriavidus are able to grow at NaCl concentrations as high as 1.5 %; and it was able to deamidate acetamide, which differentiated it from all other species of Cupriavidus. DNA–DNA hybridization data revealed low levels of genomic DNA similarity (less than 30 %) between strain CPDB6T and the type strains of Cupriavidus species with validly published names. The major cellular fatty acids detected were cis-9-hexadecenoic (16 : 1ω7c) and hexadecanoic (16 : 0) acids. On the basis of phenotypic and genotypic characterizations, strain CPDB6T was recognized as a representative of a novel species within the genus Cupriavidus. The name Cupriavidus pampae sp. nov. is proposed, with strain CPDB6T (=CCUG 55948T=CCM-A-29:1289T) as the type strain.

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Evaluating of chlorpyrifos-degrading by bacterial strains in mineral salt minimum and in the soil

ENGINEERING AND TECHNOLOGY ◽

10.46223/hcmcoujs.tech.en.10.1.358.2020 ◽

2020 ◽

Vol 10 (1) ◽

pp. 37-42

Author(s):

Truong Quoc Tat ◽

Duong Minh Vien

Keyword(s):

Agricultural Soil ◽

Mineral Salt Medium ◽

Bacterial Species ◽

Mineral Salt ◽

Soil Types ◽

Achromobacter Xylosoxidans ◽

Soil Environment ◽

Bacterial Strains ◽

Sterile Soil ◽

Staphylococcus Pasteuri

Four bacterial strains degraded chlorpyrifos, isolated from agricultural soil, were used as a source of bacteria to investigate their ability to decompose chlorpyrifos in mineral salt minimum and the soil. Barrientosimonas humi C4.3 was investigated for the decomposition of chlorpyrifos in this strain on different days (10, 20 and 30 days of culture) as supplemented and not supplemented TSB. At the same time, another experiment was carried out to evaluate the chlorpyrifos etherification of B. humi C4.3 and the four strains of Achromobacter xylosoxidans C3.1, B. humi C4.3, Microbacterium sp. C8.9, Staphylococcus pasteuri C9.2 in a soil environment. The experiment was carried out including 3 treatments, each treatment was repeated 3, two soil types (sterile soil and non-sterile soil) and bacteria (single bacteria and four bacterial species). The results showed that, in the same culture period of 30 days incubation, biodegradable chlorpyrifos of B. humi C4.3 in the mineral salt medium was more effective (63.07% biodegradable chlorpyrifos) than when grown in soil (21.4% biodegradable chlorpyrifos). Also, biodegradable chlorpyrifos of B. humi C4.3 that was cultured in sterile soil was higher than in non-sterile soil.

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Biodegradation of methyl and butylparaben by bacterial strains isolated from amended and non-amended agricultural soil. Identification, behavior and enzyme activities of microorganisms

Journal of Environmental Management ◽

10.1016/j.jenvman.2019.05.122 ◽

2019 ◽

Vol 245 ◽

pp. 245-254

Author(s):

Belén Juárez-Jiménez ◽

Chiara Pesciaroli ◽

Paula Maza-Márquez ◽

Sergio López-Martínez ◽

José Luís Vílchez-Quero ◽

...

Keyword(s):

Enzyme Activities ◽

Agricultural Soil ◽

Bacterial Strains

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Combined Effect of Laboratory-Simulated Fire and Chromium Pollution on Microbial Communities in an Agricultural Soil

Biology ◽

10.3390/biology10070587 ◽

2021 ◽

Vol 10 (7) ◽

pp. 587

Author(s):

Ida Rascio ◽

Maddalena Curci ◽

Concetta Eliana Gattullo ◽

Anna Lavecchia ◽

Mohammad Yaghoubi Khanghahi ◽

...

Keyword(s):

Soil Properties ◽

Microbial Communities ◽

Relative Abundance ◽

Agricultural Soil ◽

Crop Residues ◽

Soil Microbial Communities ◽

Soil Conditions ◽

Rrna Gene ◽

Bacterial Strains ◽

Soil Microbial

Fire events in agricultural soils can modify not only soil properties but also the structure of soil microbial communities, especially in soils containing high concentrations of potentially toxic elements (PTEs). The recolonization of burned soils can in fact favor the proliferation of certain microorganisms, more adaptable to post-fire soil conditions and higher PTE availability, over others. In this study, we simulated with laboratory experiments the microbial recolonization of an agricultural soil containing high Cr concentrations after heating at 500 °C for 30 min, to mimic the burning of crop residues. Changes in soil properties and Cr speciation were assessed, as well as soil microbial structure by means of 16S rRNA gene sequencing. Both altered soil conditions and increased Cr availability, especially Cr(VI), appeared to be responsible for the reduction in species diversity in heated soils and the proliferation of Firmicutes. Indeed, already after 3 days from the heat treatment, Firmicutes increased from 14% to 60% relative abundance. In particular, Paenibacillus was the most abundant genus identified after the simulation, with an average relative abundance of 40%. These bacteria are known to be good fire-responders and Cr-tolerant. These results could be useful to identify bacterial strains to be used as bioindicators of altered environments and for the recovery of fire-impacted polluted sites.

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Soil bacteria as a basis for sustainable development of the environment

E3S Web of Conferences ◽

10.1051/e3sconf/202124701051 ◽

2021 ◽

Vol 247 ◽

pp. 01051

Author(s):

Vasili Travkin ◽

Dilmurod Morudullaev ◽

Irina Artemyeva ◽

Nataliya Suzina ◽

Inna Solyanikova

Keyword(s):

Sustainable Development ◽

Soil Bacteria ◽

Agricultural Soil ◽

Significant Contribution ◽

Sole Source ◽

Soil Samples ◽

Bacterial Strains ◽

Persistent Pollutants ◽

Significant Interest ◽

Stimulate Plant Growth

Soil is an inexhaustible source of microorganisms of significant interest to biotechnology. Bacteria are able to control the growth of pathogenic microflora, stimulate plant growth, and decompose pollutants of varying degrees of toxicity. Bacteria make a significant contribution to the cycle of substances. The aim of this work was to isolate aerobic microorganisms from soil samples of two types - forest, without technogenic history, and agro-industrial, and to evaluate their properties. 15 strains of bacteria were isolated from forest soil, of which representatives of the genera Rhodococcus, Bacillus, Arthrobacter, Paenibacillus, Pseudomonas, Acinetobacter were able to degrade such persistent pollutants as chlorophenols, biphenyl and naphthalene. 77 strains were isolated from chernozem, of which 15 used benzoate as the sole source of carbon and energy. Thus, it has been shown that bacterial strains isolated from both forest and agricultural soil have a certain biodegradative activity, which allows them to reduce the level of pollution.

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