High density genetic mapping of stripe rust resistance in a ‘Strongfield’/‘Blackbird’ durum wheat population

Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most devastating fungal diseases of wheat worldwide. It is essential to discover more sources of stripe rust resistance genes for wheat breeding programs. Specific locus amplified fragment sequencing (SLAF-seq) is a powerful tool for the construction of high-density genetic maps. In this study, a set of 200 recombinant inbred lines (RILs) derived from a cross between wheat cultivars Chuanmai 42 (CH42) and Chuanmai 55 (CH55) was used to construct a high-density genetic map and to identify quantitative trait loci (QTLs) for stripe rust resistance using SLAF-seq technology. A genetic map of 2828.51 cM, including 21 linkage groups, contained 6732 single nucleotide polymorphism markers (SNP). Resistance QTLs were identified on chromosomes 1B, 2A, and 7B; Qyr.saas-7B was derived from CH42, whereas Qyr.saas-1B and Qyr.saas-2A were from CH55. The physical location of Qyr.saas-1B, which explained 6.24–34.22% of the phenotypic variation, overlapped with the resistance gene Yr29. Qyr.saas-7B accounted for up to 20.64% of the phenotypic variation. Qyr.saas-2A, a minor QTL, was found to be a likely new stripe rust resistance locus. A significant additive effect was observed when all three QTLs were combined. The combined resistance genes could be of value in breeding wheat for stripe rust resistance.

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Genetic mapping of a putative Thinopyrum intermedium-derived stripe rust resistance gene on wheat chromosome 1B

Theoretical and Applied Genetics ◽

10.1007/s00122-014-2261-7 ◽

2014 ◽

Vol 127 (4) ◽

pp. 843-853 ◽

Cited By ~ 30

Author(s):

Q. Huang ◽

X. Li ◽

W. Q. Chen ◽

Z. P. Xiang ◽

S. F. Zhong ◽

...

Keyword(s):

Genetic Mapping ◽

Resistance Gene ◽

Stripe Rust ◽

Rust Resistance ◽

Wheat Chromosome ◽

Rust Resistance Gene ◽

Stripe Rust Resistance ◽

Thinopyrum Intermedium ◽

Stripe Rust Resistance Gene

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Quantitative Trait Loci Mapping of Adult Plant and Seedling Resistance to Stripe Rust (Puccinia striiformis Westend.) in a Multiparent Advanced Generation Intercross Wheat Population

Frontiers in Plant Science ◽

10.3389/fpls.2021.684671 ◽

2021 ◽

Vol 12 ◽

Author(s):

Sandra Rollar ◽

Manuel Geyer ◽

Lorenz Hartl ◽

Volker Mohler ◽

Frank Ordon ◽

...

Keyword(s):

Stripe Rust ◽

Resistance Genes ◽

Quantitative Trait ◽

Rust Resistance ◽

Puccinia Striiformis ◽

Stripe Rust Resistance ◽

Adult Plant ◽

Seedling Resistance ◽

Wheat Population ◽

Advanced Generation

Stripe rust caused by the biotrophic fungus Puccinia striiformis Westend. is one of the most important diseases of wheat worldwide, causing high yield and quality losses. Growing resistant cultivars is the most efficient way to control stripe rust, both economically and ecologically. Known resistance genes are already present in numerous cultivars worldwide. However, their effectiveness is limited to certain races within a rust population and the emergence of stripe rust races being virulent against common resistance genes forces the demand for new sources of resistance. Multiparent advanced generation intercross (MAGIC) populations have proven to be a powerful tool to carry out genetic studies on economically important traits. In this study, interval mapping was performed to map quantitative trait loci (QTL) for stripe rust resistance in the Bavarian MAGIC wheat population, comprising 394 F6 : 8 recombinant inbred lines (RILs). Phenotypic evaluation of the RILs was carried out for adult plant resistance in field trials at three locations across three years and for seedling resistance in a growth chamber. In total, 21 QTL for stripe rust resistance corresponding to 13 distinct chromosomal regions were detected, of which two may represent putatively new QTL located on wheat chromosomes 3D and 7D.

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