ALEX2 multiplex examination – results of specific IgE to fish and shrimps in patients suffering from atopic dermatitis

Background and aim: Progress in laboratory diagnostics of IgE-mediated allergy is the use of component-resolved diagnosis. Our study analyses the results of specific IgE to 295 allergen reagents (117 allergenic extracts and 178 molecular components) in patients suffering from atopic dermatitis (AD) with the use of ALEX2 Allergy Explorer. Method: The complete dermatological and allergological examination, including the examination of the sensitization to molecular components with ALEX2 Allergy Explorer testing, was performed. The statistical analysis of results was performed with these methods: TURF (total unduplicated reach and frequency), best reach and frequency by group size, two-sided tests, Fisher’s exact test, and chi-square test (at an expected minimum frequency of at least 5). Results: Altogether, 100 atopic dermatitis patients were examined: 48 men, 52 women, the average age 40.9 years, min. age 14 years, max. age 67 years. The high and very high level of specific IgE was reached in 75.0% of patients to 18 molecular components: from PR-10 proteins (Aln g 1, Bet v 1, Cor a1.0103, Cor a1.0401, Fag s 1), lipocalin (Can f 1), NPC2 family (Der f 2, Der p 2), uteroglobin (Fel d 1), from Alternaria alternata (Alt a 1), Beta expansin (Lol p 1, Phl p 1), molecular components from Timothy, cultivated rye (Secc pollen) and peritrophin-like protein domain Der p 23. The high and very high level of specific IgE to other lipocalins (Fel d 7, Can f 4), to arginine kinase (Bla g 9, German cockroach), and to allergen extracts Art v (mugwort), and Cyn d (Bermuda grass) reached 52.0% of patients. The severity of AD is in significant relation to the sensitization to molecular components of storage mites (Gly d 2, Lep d 2—NPC2 family), lipocalins (Can f 1, Can f 2, Can f 4, and Can f 6), arginine kinase (Asp f 6, Bla g 9, Der p 20, Pen m 2), uteroglobin (Fel d 1, Ory c 3), Mn superoxide dismutase (Mala s 11), PR-10 proteins (Fag s 1, Mal d 1, Cor a 1.0401, Cor a 1.0103), molecular components of the peritrophin-like domain (Der p 21, Der p 23), and to Secc pollen. In the subgroup of patients suffering from bronchial asthma, the significant role play molecular components from house dust mites and storage mites (Lep d 2, Der p 2, Der f 2—NPC2 family), cysteine protease (Der p 1), peritrophin-like protein domain (Der p 21, Der p 23), enolase from Alternaria alternata (Alt a 6), and Beta expansin Phl p 1. Conclusion: The results of our study demonstrate the detailed profile of sensitization to allergens reagents (allergen extract and molecular components) in patients with atopic dermatitis. We show the significance of disturbed epidermal barrier, resulting in increased penetration of allergens. We confirmed the significant relationship between the severity of AD, the occurrence of bronchial asthma and allergic rhinitis, and high levels of specific IgE to allergen reagents. Our results may be important for regime measures and immunotherapy; Der p 23 shall be considered as an essential component for the diagnosis and specific immunotherapy of house dust mite allergy.

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Egg allergy in atopic dermatitis patients-analysis of results of specific extract IgE and specific IgE to molecular components. Bird-egg syndrome

Food and Agricultural Immunology ◽

10.1080/09540105.2021.1924631 ◽

2021 ◽

Vol 32 (1) ◽

pp. 265-282

Author(s):

Jarmila Čelakovská ◽

J. Bukač ◽

R. Vaňková ◽

J. Krejsek ◽

I. Krcmova ◽

...

Keyword(s):

Atopic Dermatitis ◽

Specific Ige ◽

Egg Allergy ◽

Analysis Of Results ◽

Molecular Components

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Racial Differences In The Relationship Of Total and Food-Specific IgE To Atopic Dermatitis In Childhood

Journal of Allergy and Clinical Immunology ◽

10.1016/j.jaci.2013.12.701 ◽

2014 ◽

Vol 133 (2) ◽

pp. AB196

Author(s):

Gillian Bassirpour ◽

Edward M. Zoratti ◽

Ganesa Wegienka ◽

Suzanne Havstad ◽

Alexandra Sitarik ◽

...

Keyword(s):

Atopic Dermatitis ◽

Racial Differences ◽

Specific Ige ◽

Food Specific Ige ◽

Relationship Of ◽

The Relationship

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CLINICAL SIGNIFICANCE OF DETERMINATION OF IGE ANTIBODIES TO STAPHYLOCOCCUS AUREUS AND MALASSEZIA SPECIES IN SERUM OF CHILDREN WITH ATOPIC DERMATITIS

Rossiyskiy Vestnik Perinatologii i Pediatrii (Russian Bulletin of Perinatology and Pediatrics) ◽

10.21508/1027-4065-2018-63-4-84-88 ◽

2018 ◽

Vol 63 (4) ◽

pp. 84-88

Author(s):

T. S. Okuneva ◽

M. V. Kushnareva ◽

A. N. Pampura ◽

E. E. Varlamov ◽

N. G. Konyukova

Keyword(s):

Staphylococcus Aureus ◽

Atopic Dermatitis ◽

Clinical Significance ◽

Specific Ige ◽

Frequency Of Occurrence ◽

Ige Antibodies ◽

Malassezia Species ◽

Specific Ige Antibodies ◽

Malassezia Spp

We studied the frequency of occurrence and concentration of allergen-specific IgE antibodies (asIgE) to enterotoxins A, B, C and TSST-1 of Staphylococcus aureus and Malassezia spp. in the serum of children with atopic dermatitis in the acute period of the disease. The determination of asIgE levels was performed in 84 children aged from 5 months to 17 years using the immunochemiluminescent method on the UniCAP 100 analyzer (Phadia, Sweden). The level of asIgE was evaluated on a scale of 6 classes.The sensitization to S. aureus enterotoxins was detected in 29 children with аsIgE concentration from 0.35 to 25.8 kU/L (I–IV classes). In 16 children were observed 8 polysensitization options to S. aureus allergens. Combinations of asIgE to A + B and A + B + C enterotoxins were most common – in every fourth and fifth child,respectively. Sensitization to Malassezia spp. was observed in 41 children with concentration from 0.38 to 98.9 KU/L (I–V classes). Polysensitization to both S. aureus and Malassezia spp. allergens was observed in one of two children. A higher degree of sensitisation to Malassezia spp allergens has been established compared to S. aureus.Conclusion. To improve the specific diagnostics of atopic dermatitis, it is advisable to examine the serum for a complete panel of recombinant S. aureus enterotoxins in combination with the determination of asIgE to Malassezia spp.

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Prevention of Transcutaneous Sensitization to Cow Milk Proteins in Infants with Atopic Dermatitis: Cohort Study

Вопросы современной педиатрии ◽

10.15690/vsp.v19i6.2152 ◽

2020 ◽

Vol 19 (6) ◽

pp. 538-544

Author(s):

Nikolay N. Murashkin ◽

Svetlana G. Makarova ◽

Stepan G. Grigorev ◽

Dmitri V. Fedorov ◽

Roman A. Ivanov ◽

...

Keyword(s):

Atopic Dermatitis ◽

Maintenance Therapy ◽

Milk Proteins ◽

Allergic Diseases ◽

Specific Ige ◽

Dispersion Analysis ◽

Cow Milk ◽

Group 2 ◽

Group 1

Background. Malformations in epidermal barrier in children with atopic dermatitis (AD) can cause transcutaneous sensitization with further development of allergic diseases that can worsen the AD course and significantly reduces patients’ quality of life.Objective. The aim of the study was to determine the effect of topical treatment and maintenance therapy with pimecrolimus 1% cream (PIM) and topical glucocorticosteroids (tGCS) in infants with AD on reducing the risk of developing transcutaneous sensitization (due to the levels of specific IgE to the cow milk protein over time) and on reducing the disease severity (by the EASI scale).Methods. The study included children aged from 1 to 4 months with early manifestations of moderate and severe AD. The severity of AD was estimated via the EASI scale at start of observation, then at 6, 9 and 12 months of life. The class and level of specific IgE to cow milk proteins (CMP) were determined by the ImmunoCAP method at the point of enrolment and at the ages of 6 and 12 months. Statistical analysis of studied indicators dynamics and their comparison in research groups was carried out using multifactorial dispersion analysis.Results. The study included 36 patients. All patients have received standard tGCS therapy in combination with emollients (wet wrap) for 10 days. The maintenance therapy was prescribed in postacute period. It included topical calcineurin inhibitor PIM 2 times/day for 3 months, then double application (morning/evening) 3 times/week up to the age of 1 year old (group 1). Other group had maintenance therapy — tGCS2 times/week for 3 months, and then at AD aggravation (group 2). Group 1 has shown lower level of sensitization to CMP at the age of 6 and 12 months and more significant decrease in AD severity according to EASI scale compared to group 2.Conclusion. The treatment with PIM is effective in therapy of AD and prevention of transcutaneous sensitization in infants.

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Chamaejasmine Isolated from Wikstroemia dolichantha Diels Suppresses 2,4-Dinitrofluoro-benzene-Induced Atopic Dermatitis in SKH-1 Hairless Mice

Biomolecules ◽

10.3390/biom9110697 ◽

2019 ◽

Vol 9 (11) ◽

pp. 697 ◽

Cited By ~ 2

Author(s):

Tae-Young Kim ◽

No-June Park ◽

Jonghwan Jegal ◽

Sangho Choi ◽

Sang Woo Lee ◽

...

Keyword(s):

Atopic Dermatitis ◽

Clinical Symptoms ◽

Skin Barrier ◽

Topical Administration ◽

Skin Lesions ◽

Specific Ige ◽

Transepidermal Water Loss ◽

Skin Barrier Function ◽

Enzyme Linked Immunosorbent Assay ◽

Dermal Thickness

Plants of the genus Wikstroemia have long been used as traditional medicines to treat diseases like pneumonia, rheumatism, and bronchitis. This study was designed to determine the effect of chamaejasmine, a biflavonoid present in W. dolichantha, on atopic dermatitis (AD)-like skin lesions in a 2,4-dinitrochlorobenzene (DNCB)-induced murine model of AD. Initially, we examined the anti-allergic activities of ten flavonoids from W. dolichantha by measuring β-hexosaminidase release from RBL-2H3 cells. Subsequently, an SKH-1 hairless mouse model of AD was developed based on the topical application of DNCB. Chamaejasmine (0.5%) or pimecrolimus (1%, positive control) were applied to dorsal skins of DNCB-sensitized AD mice for two weeks. Serum IL-4 and IgE levels were determined using enzyme-linked immunosorbent assay kits and transepidermal water loss (TEWL) and skin hydration were measured using a Tewameter TM210 and a SKIN-O-MAT, respectively. Of the ten flavonoids isolated from W. dolichantha, chamaejasmine most potently inhibited DNP-specific IgE-induced degranulation in RBL-2H3 cells. Topical administration of chamaejasmine attenuated the clinical symptoms of DNCB-induced dermatitis (i.e., itching, dryness, erythema, and edema). Histological analyses demonstrated that dermal thickness and mast cell infiltration in dermis were significantly reduced by chamaejasmine. In addition, 0.5% chamaejasmine inhibited DNCB-induced increases in total IL-4 and IgE levels in serum, improved skin barrier function, and increased epidermis moisture. Our findings suggest chamaejasmine might be an effective therapeutic agent for the treatment of atopic diseases.

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House dust mite sensitisation and association with atopic dermatitis in Brunei

Clinical and Translational Allergy ◽

10.1186/s13601-019-0304-5 ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 1

Author(s):

Haziq Emran ◽

Christina Siew Eng Chieng ◽

Surita Taib ◽

Anne Catherine Cunningham

Keyword(s):

Atopic Dermatitis ◽

House Dust ◽

House Dust Mite ◽

Retrospective Cohort ◽

Specific Ige ◽

Dermatophagoides Pteronyssinus ◽

Food Allergens ◽

Dust Mite ◽

Storage Mite ◽

Tropical Areas

AbstractIgE sensitisation in tropical areas is under-reported. A 2 year retrospective cohort study of allergy data specific to aero and food allergens in Brunei demonstrated that specific IgE levels to house dust mite (Dermatophagoides pteronyssinus, D.farinae, Blomia tropicalis) were highest in this population and correlated with atopic dermatitis (p < 0.001). Shrimp and peanut were the most common food allergens. A dominance of house/storage mite sensitization is seen in Brunei which is consistent with other tropical countries.

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