Low-dose hypersensitivity of V79 cells under exposure to γ-rays and 4 He ions of different energies: survival and chromosome aberrations

In this study, mints were regenerated from calluses irradiated by γ - rays (Co-60) at doses from 0 to 70 Gy. After 4 times of propagation, the M1V4 mints were transferred in a greenhouse for assessments of growth, development and essential oil content. Mint regenerated from calluses irradiated at low doses (0, 3, 4, 6, 8 and 10 Gy) showed no morphological difference compared to the control plants. However, low-dose irradiated mints showed better effect on growth and development, and mints regenerated from 6Gy-irradiated sample had essential oil content with 1.3 times higher than that of control group. In case of plants irradiated at higher dose (30, 40, 50, 60 and 70 Gy), there were morphological variations such as stem become bigger and color of stem become purple. Especially, samples irradiated at 60 Gy showed better in growth, development and essential oil content (1.34 times higher than the control plants) and these characters were stable in M1V4. Among the irradiated mints, purple stem variations had the highest essential oil yield with 1.7 times higher than that of the original plants.

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Evidence for Differences among the Sectors of Potentially Lethal Damage Expressed by Hypertonic Treatment in Plateau-Phase V79 Cells after Exposure to Neutrons or γ Rays: The Importance of Distinction between α and β-PLD Forms

Radiation Research ◽

10.2307/3576966 ◽

1986 ◽

Vol 108 (1) ◽

pp. 23 ◽

Cited By ~ 5

Author(s):

G. Iliakis ◽

E. Wright ◽

W. K. Roberts ◽

F. Q. H. Ngo

Keyword(s):

Plateau Phase ◽

V79 Cells ◽

Lethal Damage ◽

Γ Rays

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Telomerase Activity, Telomere Length, and Chromosome Aberrations in the Extension of Life Span of Human Embryo Cells Induced by Low-dose X-rays.

Journal of Radiation Research ◽

10.1269/jrr.39.35 ◽

1998 ◽

Vol 39 (1) ◽

pp. 35-51 ◽

Cited By ~ 15

Author(s):

ZHI YANG ◽

SEIJI KODAMA ◽

KEIJI SUZUKI ◽

MASAMI WATANABE

Keyword(s):

Life Span ◽

Telomere Length ◽

Human Embryo ◽

Chromosome Aberrations ◽

Low Dose ◽

Telomerase Activity ◽

X Rays ◽

Embryo Cells

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Analysis of γ-rays induced chromosome aberrations: A fingerprint evaluation with a combination of pan-centromeric and pan-telomeric probes

International Journal of Radiation Biology ◽

10.1080/09553000600979092 ◽

2006 ◽

Vol 82 (12) ◽

pp. 869-875 ◽

Cited By ~ 9

Author(s):

L. Benkhaled ◽

L. Barrios ◽

M. Mestres ◽

M. R. Caballin ◽

M. Ribas ◽

...

Keyword(s):

Chromosome Aberrations ◽

Γ Rays

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The Rejoining Time of Chromatid Breaks Induced by Gamma Radiation in Vicia faba Root Tips at 3 °C

The Journal of Cell Biology ◽

10.1083/jcb.7.1.79 ◽

1960 ◽

Vol 7 (1) ◽

pp. 79-85 ◽

Cited By ~ 5

Author(s):

J. R. K. Savage ◽

G. J. Neary ◽

H. J. Evans

Keyword(s):

Root Growth ◽

Vicia Faba ◽

Low Dose ◽

Root Meristem ◽

Root Tips ◽

Dose Rates ◽

Low Dose Rate ◽

Chromatid Aberration ◽

Γ Rays ◽

Chromatid Aberrations

The observation was made previously that the reduction in radiosensitivity in Vicia faba (as measured by postirradiation root growth) by prolonging the exposure time from about 10 minutes to 24 hours is much less marked at 3°C. than at 19°C. If chromosome damage is mainly responsible for the reduced root growth, this observation might be explained by a smaller drop in the "two-hit" aberration component, resulting from an increased time for which breaks are available for rejoining at 3°C. This hypothesis was tested by comparing chromatid aberration frequencies in root meristem cells produced by 105 rads of 60Co γ rays, given at dose rates of 19.4 and 0.073 rads per minute. Beans were maintained in aerated water at 2°C. prior to and during irradiation, and at this temperature the rate of development of cells was such that the two different exposure times both occupied a period during which the cell sensitivity was approximately constant. Immediately subsequent to irradiation, the roots were returned to 19°C. and examined cytologically. All chromatid aberrations were less frequent after low dose rate treatment, but only the chromatid interchange reduction was significant. The average time for which breaks are available for reunion, calculated from Lea's G function, was found to be 12 hours (95 per cent C.L. 6 to 24 hours).

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