Effect of toxoplasmic infection on the biodistribution of a brain radiopharmaceutical

2019 ◽  
Vol 95 (11) ◽  
pp. 1547-1551
Author(s):  
João Cláudio da Costa Urbano ◽  
Ranny Beatriz de Carvalho Holanda Leite ◽  
Renan Leopoldo Pereira Castro ◽  
Clarice Maux Vianna da Silva ◽  
Joelma Maria de Araújo Andrade ◽  
...  
Keyword(s):  
Toxoplasmic Infection

scholarly journals Histopathological analysis of the reproductive system of male dogs experimentally infected with Toxoplasma gondii

2009 ◽  
Vol 39 (7) ◽  
pp. 2123-2127 ◽  
Author(s):  
Tiago Pereira Arantes ◽  
Welber Daniel Zanetti Lopes ◽  
Roberta Machado Ferreira ◽  
Juliana de Souza Pinto Pieroni ◽  
Vanessa Marigo Rocha Pinto ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Reproductive System ◽  
Interstitial Fibrosis ◽  
Optical Microscope ◽  
Seminiferous Tubules ◽  
Histopathological Analysis ◽  
Hydropic Degeneration ◽  
Toxoplasmic Infection ◽  
Toxoplasma Gondii Infection ◽  
Histopathological Results

The present research aimed to describe possible histopathological alterations in the reproductive system (testicles and epididymis) of male dogs experimentally infected with Toxoplasma gondii. Canines (n=10) serologically negative for T. gondii were selected and distributed into three experimental groups: GI, 3 inoculated with 2.0 x 10(5)P strain oocysts; GII, 3 infected with 1.0 x 10(6)RH strain tachyzoites; and GIII, 4 control dogs. Antibody research (IFAT) against T. gondii was realized. Toxoplasma gondii infection was confirmed by seroconversion of the 6 males infected with tachyzoites and oocysts from postinoculation day (PID) 7 and 14, respectively. At PID 70, all dogs were submitted to orchiectomy and testicle and epididymis samples were collected and histologically processed for examination under optical microscope. The following alterations were diagnosed: mild and moderate mononuclear inflammatory infiltrate in the epididymis, moderate cellular edema, hydropic degeneration and moderate interstitial fibrosis in seminiferous tubules. The histopathological results in the present research, isolation of T. gondii in testicle and epididymis fragments by immunohistochemistry and results from the literature by other authors in different tissues, all infer that the alterations observed in dogs infected with T. gondii are suggestive of toxoplasmic infection.

scholarly journals Evolution of IgG antibody response against Toxoplasma gondii tissue cyst in acute and chronic human infections

1998 ◽  
Vol 40 (2) ◽  
pp. 77-84 ◽  
Author(s):  
Margarita VILLAVEDRA ◽  
Hernán CAROL ◽  
Alberto NIETO
Keyword(s):  
Toxoplasma Gondii ◽  
Acute Infection ◽  
Chronic Phase ◽  
Tissue Cyst ◽  
Specific Response ◽  
Igg Antibodies ◽  
Igg Antibody ◽  
Wide Range ◽  
Toxoplasmic Infection ◽  
Human Infections

The recognition profile of the tissue cysts antigens by IgG antibodies was studied during acute and chronic human toxoplasmic infection. Thus the IgG response against Toxoplasma gondii was investigated by immunoblotting in two patients accidentally infected with the RH strain as well as in group of naturally infected patients at acute and chronic phase. There was an overall coincidence of molecular mass among antigens of tachyzoites and tissue cysts recognized by these sera, however, they appear not to be the same molecules. The response against tissue cysts starts early during acute infection, and the reactivity of antibodies is strong against a wide range of antigens. Six bands (between 82 and 151 kDa) were exclusively recognized by chronic phase sera but only the 132 kDa band was positive in more than 50% of the sera analysed. A mixture of these antigens could be used to discriminate between the two infection phases. The most important antigens recognized by the acute and the chronic phase sera were 4 clusters in the ranges 20-24 kDa, 34-39 kDa, 58-80 kDa and 105-130 kDa as well as two additional antigens of 18 and 29 kDa. Both accidentally infected patients and some of the naturally infected patients showed a weak specific response against tissue cyst antigens.

scholarly journals Toxoplasmic Infection-induced Injury in the Ileal Myenteric Plexus in Rats Depends on the Dose of Toxoplasma gondii Oocysts

2018 ◽  
Vol 35 (01) ◽  
pp. 80-86
Author(s):  
L. Tironi ◽  
E. Beraldi ◽  
S. Borges ◽  
C. Massocato ◽  
S. Vieira ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Myenteric Plexus ◽  
Neuronal Population ◽  
Neuronal Morphology ◽  
Control Group ◽  
Enteric Glial Cells ◽  
Male Wistar Rats ◽  
Quantitative Analyses ◽  
Neuronal Hypertrophy ◽  
Toxoplasmic Infection

Introduction The present study evaluated the effects of different inocula of Toxoplasma gondii oocysts on the ileal myenteric plexus in rats. Materials and Methods Male Wistar rats, 60 days old, were distributed into four groups: control group (CG; which received saline solution) and groups that were infected with 100 (TG100), 1000 (TG1000), and 5000 (TG5000) T. gondii oocysts. Thirty days after infection, the rats were sacrificed, and the ileum was collected to make whole-mount preparations that were subjected to immunofluorescence staining to observe the general neuronal population (HuC/D), nitrergic neurons (nNOS), and enteric glial cells (S100). Morphometric and quantitative analyses of myenteric neurons were performed. Results The infections with different T. gondii inocula did not cause neuronal or glial loss, but cause neuronal hypertrophy in general population and nitrergic subpopulation in infected groups. Conclusion: Changes in neuronal morphology were observed in the TG5000 group, including the presence of vacuoles, translocation of Hu protein to the nucleus, and dendritic distortions, suggesting functional alterations in these cells.

Minocycline in the chemotherapy of murine toxoplasmosis

Parasitology ◽  
1982 ◽  
Vol 84 (2) ◽  
pp. 297-302 ◽  
Author(s):  
K. F. Tabbara ◽  
S. Sakuragi ◽  
G. R. O'connor
Keyword(s):  
Toxoplasma Gondii ◽  
Combination Therapy ◽  
Virulent Strain ◽  
Toxoplasmic Infection ◽  
Acute Toxoplasmosis

SUMMARYThe effects of minocycline alone or in combination with sulphadiazine were evaluated against acute murine toxoplasmosis and compared with the efficacy of pyrimethamine and sulphadiazine. The data presented show that mice could be protected from acute toxoplasmosis by minocycline, in spite of using a virulent strain of Toxoplasma gondii, a large inoculum, and a delay in the administration of the drug. Acute toxoplasmic infection in mice could be eradicated by a minocycline–sulphadiazine combination, which was found to be as effective as pyrimethamine–sulphadiazine combination therapy.

Toxoplasmic infection in cardiac disease

1979 ◽  
Vol 43 (4) ◽  
pp. 841-849 ◽  
Author(s):  
David Leak ◽  
Mohammed Meghji
Keyword(s):  
Cardiac Disease ◽  
Toxoplasmic Infection

scholarly journals Combination of ELISA and RT-PCR tests in the diagnosis of toxoplasmic infection in aborted women and congenitally infected infants.

2014 ◽  
Vol 8 (3) ◽  
pp. 44-47
Author(s):  
Khitam Y. Obaid AL-Dujaily ◽  
Noor SH. Abdul-Amir
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Good Combination ◽  
Igg Antibodies ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Toxoplasmic Infection ◽  
Sera Samples

The diagnosis of toxoplasmic infection in aborted women and congenitally infected infants suspected to have toxoplasmosis infection can be difficult due to similarity symptoms with other diseases. A combination of symptoms, serology and polymerase chain reaction (PCR)may facilitate diagnosis of toxoplasmosis in some patients. The present study compare the detection of toxoplasmosis infection by ELISA IgA and IgG antibodies with Real Time polymerase chain reaction (RT-PCR)in the study subjects. A total of 81 sera samples, 57(70.3%) samples from aborted women and 24(29.7%)samples from congenitally infants have been studied. 49(86%) samples from the aborted women were positive and 8(14%) samples were negative as diagnosed by one or two of ELISA markers (IgAand IgG).The ELISA results indicated that 15(62.5%) samples from infants were positive and 9(37.5%) samples of them were negative. RT-PCR tests indicated that 33(67.3%) from the mothers and 6(40%) from the infants were agreed with ELISA positive samples. For ELISA negative samples, RT-PCR detected toxoplasmosis DNA in 4 (50%) and 2 (22.2%) for the mothers and infants respectively. Therefore, ELISA and RT-PCR can make a good combination tests in detection toxoplasmopsis infection.

scholarly journals Myenteric neuronal plasticity induced by Toxoplasma gondii (genotype III) on the duodenum of rats

2012 ◽  
Vol 84 (3) ◽  
pp. 737-746 ◽  
Author(s):  
Rodrigo M. Papazian-Cabanas ◽  
Eduardo J.A. Araújo ◽  
Aristeu V. da Silva ◽  
Débora M.G. Sant'Ana
Keyword(s):  
Toxoplasma Gondii ◽  
Neuronal Loss ◽  
Chronic Phase ◽  
The Body ◽  
Control Group ◽  
Myenteric Neurons ◽  
Cytoplasmic Area ◽  
Genotype Iii ◽  
Toxoplasmic Infection ◽  
Experimental Group

The effects of acute and chronic infection caused by Toxoplasma gondii on duodenal myenteric neurons were analyzed. Eighteen rats were assigned into four groups: Acute Control Group (ACG, n=4); Acute Experimental Group (AEG, n=4); Chronic Control Group (CCG, n=5); and Chronic Experimental Group (CEG, n=5). Rats from the AEG and CEG were inoculated orally with 105 genotype III (BTU-II strain) tachyzoites of T. gondii isolated from a dog with neurological signs. Acute groups were killed after 24 hours after the inoculation and the chronic groups after 30 days. Whole-mount from the duodenum were stained with Giemsa. The population density of myenteric neurons, as well the body cell, nuclear and cytoplasmic area were analyzed. Both acute and chronic toxoplasmic infection did not provoke neuronal loss. On the other hand, plastic alterations were observed: decreasing of the nuclear and cytoplasmic area during the acute phase and neuronal hypertrophy during the chronic phase.

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