Waste frying oil hydrolysis and lipase production by cold-adapted Pseudomonas yamanorum LP2 under non-sterile culture conditions

The search for innovation and biotechnological strategies in the biodiesel production chain have become a topic of interest for scientific community owing the importance of renewable energy sources. This work aimed to implement an enzymatic transesterification process to obtain biodiesel from waste frying oil (WFO). The transesterification was performed by varying reaction times (8 h, 12 h and 16 h), enzyme concentrations of lipase XX 25 split (14%, 16% and 18%), pH of reaction media (6, 7 and 8) and reaction temperature (35, 38 and 40 °C) with a fixed alcohol–oil molar ratio of 3:1. The optimum operating conditions were selected to quantify the amount of fatty acid methyl esters (FAMEs) generated. The highest biodiesel production was reached with an enzyme concentration of 14%, reaction time of 8 h, pH of 7 and temperature of 38 °C. It was estimated a FAMEs production of 42.86% for the selected experiment; however, best physicochemical characteristics of biodiesel were achieved with an enzyme concentration of 16% and reaction time of 8 h. Results suggested that enzymatic transesterification process was favorable because the amount of methyl esters obtained was similar to the content of fatty acids in the WFO.

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Microwave assisted transesterification of waste frying oil and concentrate methyl ester content of biodiesel by molecular distillation

Fuel ◽

10.1016/j.fuel.2012.06.084 ◽

2013 ◽

Vol 104 ◽

pp. 614-619 ◽

Cited By ~ 45

Author(s):

Nezihe Azcan ◽

Ozlem Yilmaz

Keyword(s):

Methyl Ester ◽

Molecular Distillation ◽

Frying Oil ◽

Waste Frying Oil ◽

Microwave Assisted ◽

Ester Content ◽

Methyl Ester Content

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Screening of Candida boidinii from Chemlal spent olive characterized by higher alkaline-cold adapted lipase production

AFRICAN JOURNAL OF BIOTECHNOLOGY ◽

10.5897/ajb2013.13586 ◽

2014 ◽

Vol 13 (11) ◽

pp. 1287-1294 ◽

Cited By ~ 2

Author(s):

Bataiche Insaf ◽

Kacem-chaouche Noreddine ◽

Destain Jacqueline ◽

Lejeune Annick ◽

Thonart Philippe

Keyword(s):

Lipase Production ◽

Candida Boidinii ◽

Cold Adapted

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Lipase production with free and immobilized cells of cold-adapted yeast Rhodotorula glutinis HL25

Biocatalysis and Agricultural Biotechnology ◽

10.1016/j.bcab.2016.08.009 ◽

2016 ◽

Vol 8 ◽

pp. 97-103 ◽

Cited By ~ 9

Author(s):

Mesut Taskin ◽

Muhammed Hanifi Ucar ◽

Yagmur Unver ◽

Ayse Aydan Kara ◽

Mustafa Ozdemir ◽

...

Keyword(s):

Immobilized Cells ◽

Rhodotorula Glutinis ◽

Lipase Production ◽

Cold Adapted

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Screening of Amazonian bacillus strains for lipase production using glycerol as substrate

ScientiaTec ◽

10.35819/scientiatec.v7i03.2560 ◽

2020 ◽

Vol 7 (03) ◽

Author(s):

Giandra Volpato ◽

Victória Furtado Migliavacca ◽

Bruna Coelho de Andrade ◽

Júlio Xandro Heck ◽

Marco Antônio Záchia Ayub

Keyword(s):

Organic Synthesis ◽

Culture Medium ◽

Lipolytic Activity ◽

Gum Arabic ◽

Lipase Production ◽

Culture Conditions ◽

Search And Selection ◽

Triton X ◽

Potential Use ◽

Selection Of

The industrial application of lipolytic enzymes has been studied mainly due to the ability of these enzymes in catalyze reactions of synthesis and their stability in various organic solvents. One possibility is the use of lipase the organic synthesis, taking advantage as the generation of waste and difficult recovery of sub bioproducts. In this work, we carried out a selection of eighty-four isolates of Bacillus amazonian for lipase production, of which 30 strains showed lipolytic activity. The study of the culture conditions was performed through a Plackett-Burman experimental design using the strain that presented the highest lipolytic activity in a culture medium using glycerol as substrate. The studied conditions were: concentration of soybean oil, olive oil, triton X-100, gum arabic, glycerol, and (NH4)2SO4, pH, temperature and concentration of inoculums. The best result obtained were 27 U/L in 48 h of cultivation by Bacillus circulans BL53. This work shows that the search and selection of microorganism with lipolytic activities can facilitate the discovery of new lipases, with potential use as by-product surplus.

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