Platelet-rich plasma for tissue regeneration can be stored at room temperature for at least five days

SummaryPlatelet acetylcholinesterase (AChE) activity was measured in gel-filtered platelet preparations. Three different anticholinesteratic agents (eserine, neostigmine, and diiso- propylphosphorofluoridate) at final concentrations of 10 μM caused complete inhibition of AChE activity after 30 min incubation at room temperature with either platelet-rich plasma or gel-filtered platelets. Complete inhibition of platelet AChE had no effect on platelet aggregation, factor-3 availability, and plasma clot retraction. We conclude that platelet membrane AChE activity is not required for normal platelet function as measured by these in vitro parameters.

Download Full-text

Effect of local administration of platelet-rich plasma and guided tissue regeneration on the level of bone resorption in early dental implant insertion

Vojnosanitetski pregled ◽

10.2298/vsp0806462d ◽

2008 ◽

Vol 65 (6) ◽

pp. 462-468 ◽

Cited By ~ 2

Author(s):

Milos Duka ◽

Zoran Lazic ◽

Marija Bubalo

Keyword(s):

Bone Resorption ◽

Bone Defect ◽

Tissue Regeneration ◽

Platelet Rich Plasma ◽

Guided Tissue Regeneration ◽

Cellular Migration ◽

Significant Difference ◽

The Right ◽

Defect Filling ◽

Deproteinized Bone

Background/Aim. Osseointegration is a result of cellular migration, differentiation, bone formation, and bone remodeling on the surface of an implant. Each of these processes depends on platelets and blood coagulum. Platelet-rich plasma (PRP) is used to improve osseointegration and stability of implants. The aim of the research was to test the influence that PRP and guided tissue regeneration in bone defects have on bone defect filling and the level of bone resorption in early implant insertion. Methods. This experimental study included 10 dogs. A total of 40 BCT implants were inserted, 4 in each dog (two on the left side and two on the right side), with guided tissue regeneration. Radiologic analyses were done immediately after the insertion and 10 weeks after the insertion. Bone defect filling was measured by a graduated probe 10 weeks after the implant insertion. The following protocols were tested: I - PRP in combination with bovine deproteinized bone (BDB) and resorptive membrane of bovine origin (RBDM), II - BDB + RBDM, III - PRP + RBDM and IV - RBDM. Results. The applied protocols affected differently the bone defect filling and the level of bone resorption. Significantly better results (the lowest bone resorption) were achieved with protocol I (PRP + BDB + RBDM) in comparison with protocols III (PRP + RBDM) and IV (RBDM), but not with protocol II (BDB + RBDM). On the other hand, no significant difference was found among protocols II (BDB + RBDM), III (PRP + RBDM) and IV (RBDM) in the level of bone tissue resorption. Conslusion. The bone defect filling was largest and the level of bone resorption was lowest in the protocol with PRP applied in combination with BDB and RBDM.

Download Full-text

Platelet rich plasma in dermatology and cosmetology

International Journal of Research in Dermatology ◽

10.18203/issn.2455-4529.intjresdermatol20200616 ◽

2020 ◽

Vol 6 (2) ◽

pp. 288

Author(s):

Shobhit Mohan ◽

Lalit Mohan ◽

Renu Sangal ◽

Neelu Singh

Keyword(s):

Wound Healing ◽

Tissue Regeneration ◽

Blood Product ◽

Platelet Rich Plasma ◽

Autologous Blood ◽

Detailed Knowledge ◽

Skin Rejuvenation ◽

Scar Revision ◽

Current Review

<p class="abstract">Platelet rich plasma (PRP) therapies in medicine has become increasing popular during the last decade. The interest in in the application of PRP in dermatology and cosmetology has increased recently in different applications such as alopecia, skin rejuvenation, wound healing, scar revision, and tissue regeneration. PRP is an autologous blood product obtained from the blood of the patients. The detailed knowledge about PRP should help clinicians better understand this therapy. In this view, the current review was done for a better understanding of what pathologies can be corrected with PRP.</p>

Download Full-text

Platelet-Rich Plasma has no Additional Benefit During Guided Tissue Regeneration Procedure to Significantly Improve Clinical Attachment Gains in the Treatment of Periodontal Intrabony Defects

Journal of Evidence Based Dental Practice ◽

10.1016/j.jebdp.2011.12.003 ◽

2012 ◽

Vol 12 (1) ◽

pp. 5-7 ◽

Cited By ~ 12

Author(s):

Jia-Hui Fu ◽

Hom-Lay Wang

Keyword(s):

Tissue Regeneration ◽

Platelet Rich Plasma ◽

Additional Benefit ◽

Guided Tissue Regeneration ◽

Intrabony Defects ◽

Regeneration Procedure

Download Full-text

Platelet Retention in Glass Bead Columns: Further Evidence for the Importance of ADP

Blood ◽

10.1182/blood.v44.3.411.411 ◽

1974 ◽

Vol 44 (3) ◽

pp. 411-425 ◽

Cited By ~ 14

Author(s):

V. J. McPherson ◽

M. B. Zucker ◽

N. M. Friedberg ◽

P. L. Rifkin

Keyword(s):

Platelet Aggregation ◽

Acetylsalicylic Acid ◽

Glass Bead ◽

Room Temperature ◽

Creatine Phosphokinase ◽

Platelet Rich Plasma ◽

Creatine Phosphate ◽

Inhibitory Effect ◽

Normal Platelet ◽

Normal Individuals

Abstract Plasma of normal heparinized blood contained 0.284 µM ± SD 0.097 (ADP + ATP) with an ATP:ADP ratio of 2.5:1. Plasma from thrombocytopenic blood contained only 0.106 µM ± 0.073 (ADP + ATP). Blood with normal platelet retention released 0.234 µM ± 0.187 (ADP + ATP) during passage through a glass bead column, with an ATP:ADP ratio of 1.6:1. Significantly less was released in blood with low retention, i.e., samples from patients with von Willebrand’s disease, thrombasthenia, or thrombocytopenia, and some samples from normal individuals. Thus, nucleotides in the plasma of pre- and postcolumn blood appear to be derived from platelets; their release within glass bead columns is closely associated with normal platelet retention. Since release occurred at room temperature and was not prevented by acetylsalicylic acid or accompanied by measurable release of 14C-serotonin, the classic release reaction may not have been responsible. The low retention in platelet-rich plasma was variably increased by adding 0.5 µM ADP, an increase at least partly due to trapping of preformed aggregates. Retention in undisturbed blood was markedly inhibited by creatine phosphokinase with creatine phosphate (CPK-CP) and moderately inhibited by apyrase I (ATPase:ADPase 0.8:1) at an ADP-removing activity between 1 and 5 U/ml, indicating that ADP is essential for retention. At less than 1 U/ml, both apyrase I and II (ATPase: ADPase 2.8:1) enhanced retention in undisturbed blood, but CPK-CP was still inhibitory. These results suggest that enhancement is due to conversion of released ATP to ADP, as shown to occur in studies of platelet aggregation with ATP and ADP. At less than 1 U/ml, all three enzymes protected against the inhibitory effect of disturbance; this protection was marked with apyrase II, moderate with apyrase I and slight with CPK-CP. These observations provide additional evidence that ADP is responsible for the low retention caused by disturbance of the blood.

Download Full-text

The effectiveness of platelet concentrations in periodontal surgeries

International Journal of Dental Research ◽

10.14419/ijdr.v6i2.12414 ◽

2018 ◽

Vol 6 (2) ◽

pp. 61 ◽

Cited By ~ 1

Author(s):

Ronad Al-Azem ◽

Neveen Ali ◽

Diana Mostafa

Keyword(s):

Case Report ◽

Growth Factors ◽

Bone Graft ◽

Tissue Regeneration ◽

Platelet Rich Plasma ◽

Research Process ◽

Platelet Concentrates ◽

Platelet Rich Fibrin ◽

Barrier Membrane ◽

Concentrated Growth Factors

Platelets release several growth factors which stimulate tissue regeneration. Several techniques for platelet concentrates such as platelet rich plasma (PRP), plasma rich in growth factors (PRGF), platelet rich fibrin (PRF) and concentrated growth factors (CGF) have been introduced in dental surgeries for the prevention of hemorrhage and acceleration of tissue regeneration. However, a fabricating growth factors-enriched bone graft matrix which is called “sticky bone” has been demonstrated to provide stabilization in bony defects. In this article, we presented the method of preparing and utilizing CGF and sticky bone and evaluate the effect of CGF mixed with bone graft and CGF barrier membrane in periodontal surgeries.Methodology: We used websites such as PubMed, Scopus, and ISI Web of knowledge to get related articles about this subject. The research process involved specific key words " concentrated growth factor”- “Platelet rich fibrin”- growth factors-enriched bone graft” - “sticky bone” to find more articles which published from 2007 to March 2018.Results: We reviewed 48 articles, 43 articles were excluded. Only five articles have been conducted. Original human studies and case report were included.Conclusion: We concluded that the use of sticky bone and CGF is effective in bone grafting and implant.

Download Full-text

Activation of the blood fibrinolytic enzyme system by platelets

American Journal of Physiology-Legacy Content ◽

10.1152/ajplegacy.1964.206.6.1255 ◽

1964 ◽

Vol 206 (6) ◽

pp. 1255-1261 ◽

Cited By ~ 9

Author(s):

W. O. Reid ◽

M. J. Silver

Keyword(s):

Fibrinolytic Activity ◽

Room Temperature ◽

Enzyme System ◽

Platelet Rich Plasma ◽

Venous Blood ◽

Aminocaproic Acid ◽

Clot Retraction ◽

Oxalate Solution ◽

Epsilon Aminocaproic Acid ◽

Inhibitor Of Plasminogen Activator

Fibrinolytic activity in human blood develops after the occurrence of viscous metamorphosis (VM) of platelets. Freshly drawn venous blood was held at room temperature until spontaneous VM of platelets occurred and then introduced into oxalate solution to prevent coagulation. As compared to the control (blood added to anticoagulant before VM) plasma of such samples exhibited increased fibrinolytic activity, detected by increased serial thrombin times. Clots from platelet-rich plasma exhibited an increase in the convergence of branches of the thrombelastograph and a decrease in weight after incubation for 24 hr or more. The increase in branch convergence was roughly proportional to the platelet count and was reduced or absent in thrombocytopenic blood or platelet-poor plasma. Dimethylsulfoxide blocked VM and prevented branch convergence (without preventing clot retraction). Epsilon-aminocaproic acid, an inhibitor of plasminogen activator, blocked or inhibited fibrinolytic activity without arresting VM. This evidence suggests that platelets contain significant proactivator or activator of plasminogen (or a factor which initiates their activity) which is released when VM occurs.

Download Full-text

A clinical study of platelet rich plasma versus conventional dressing in management of diabetic foot ulcers

International Surgery Journal ◽

10.18203/2349-2902.isj20184069 ◽

2018 ◽

Vol 5 (10) ◽

pp. 3210 ◽

Cited By ~ 1

Author(s):

Nithyaraj Prakasam ◽

Prabakar M.S. ◽

Reshma S. ◽

Loganathan K. ◽

Senguttuvan K.

Keyword(s):

Wound Healing ◽

Tissue Regeneration ◽

Diabetic Foot ◽

Tamil Nadu ◽

Chronic Wounds ◽

Platelet Rich Plasma ◽

Foot Ulcers ◽

Diabetic Foot Ulcers ◽

Group A ◽

Group B

Background: Diabetic foot ulcers continue to pose significant global issue despite the advances made in the management of diabetes. It causes major foot complications if they are not addressed properly. It needs multidisciplinary approach for its care. While several advancements has taken place in wound care management, platelet rich plasma and stem cell therapy promises to offer a new hope in its management, aiding in cellular and tissue regeneration. The purpose of the present study was to compare Platelet-Rich Plasma versus conventional dressing in the management of diabetic foot ulcers.Methods: This prospective study was focused on 20 diabetic foot ulcers, carried out in a surgical unit of ACS Medical College and Hospital, Chennai, Tamil Nadu, from January 2018 to June 2018. Patients were divided into two groups; Group A received conventional ordinary dressing (N=10, 50%) and Group B received PRP dressing (N = 10, 50%). The mean follow-up period was 8 weeks.Results: The estimated time of wound healing was 8 weeks and healing was found to be more effective for patients in group B compared to patients in group A; the PRP group was found to be more effective in wound healing with fewer complications, less infection, exudates and pain.Conclusions: There have been considerable advances in the use of PRP in therapeutic processes in recent years in tissue regeneration therapy. PRP is a powerful tool for the treatment of chronic wounds and very promising for diabetic foot wounds; PRP enables healing, and reduces amputation rates, infection and exudates.

Download Full-text

The Effect of Cold on Platelets. II. Platelet Function After Short-term Storage at Cold Temperatures

Blood ◽

10.1182/blood.v40.5.688.688 ◽

1972 ◽

Vol 40 (5) ◽

pp. 688-696 ◽

Cited By ~ 45

Author(s):

Herman E. Kattlove ◽

Benjamin Alexander ◽

Frances White

Keyword(s):

Platelet Aggregation ◽

Room Temperature ◽

Platelet Rich Plasma ◽

Adenosine Diphosphate ◽

Serotonin Release ◽

Prolonged Storage ◽

Cold Temperatures ◽

Aggregation Of Platelets ◽

Term Storage

Abstract Citrated platelet-rich plasma (PRP) was kept at cold temperatures or room temperature. After 4 hr or more at these temperatures, the PRPs were warmed 1 hr at 37°C. This prevents the spontaneous aggregation seen in chilled PRP that is stirred immediately after warming. Platelet aggregation in response to connective tissue (CT), epinephrine, and adenosine diphosphate (ADP) was considerably greater in the PRPs originally kept at cold temperatures. In addition, chilling would restore the aggregation of platelets whose function had deteriorated due to prolonged storage at warm temperatures. Neither ADP-induced refractoriness, serotonin uptake, or CT-induced serotonin release was affected by cold. Retention in glass bead columns was greater in platelets that had been chilled than in platelets kept at room temperature or 37°C. Thus, the storage of platelets at cold temperatures leads to changes that improve platelet aggregation but may also increase platelet adhesion, which would account for the decreased in vivo survival of platelets preserved for transfusion at cold temperatures.

Download Full-text