Topical administration of hydroethanolic extract of Trifolium pratense (red clover) accelerates wound healing by apoptosis and re-epithelialization

2020 ◽  
pp. 1-11
Author(s):  
R. Manzoureh ◽  
M. R. Farahpour
Keyword(s):  
Wound Healing ◽  
Trifolium Pratense ◽  
Red Clover ◽  
Topical Administration ◽  
Hydroethanolic Extract

The Effect of Topical Administration of an Ointment Prepared From Trifolium repens Hydroethanolic Extract on the Acceleration of Excisional Cutaneous Wound Healing

2020 ◽  
pp. 253-261
Keyword(s):  
Antioxidant Activity ◽  
Wound Healing ◽  
Mast Cell ◽  
Cell Lymphoma ◽  
B Cell Lymphoma ◽  
Topical Administration ◽  
Control Group ◽  
Cell Distribution ◽  
Carbohydrate Storage ◽  
Hydroethanolic Extract

Introduction: Natural agents with antioxidant and anti-inflammatory properties are safer than synthetic agents and may improve wound healing. Objective: The purpose of this study is to evaluate the in vivo wound healing potential of an ointment prepared from Trifolium repens hydroethanolic extract (T repens) concerning excisional wounds in a rat model. Materials and methods: Seventy-two adult Wistar rats were divided into 4 groups: a control group and 3 groups of animals treated with 1.5% T repens, 3% T repens, and 6% T repens. A full-thickness wound with an area of 314 mm2 was created in each rat. To investigate the effect of T repens on wound healing, the wound area, histological analyses (eg, angiogenesis, fibroblast, fibrocyte, mast-cell distribution), intracytoplasmic carbohydrate storage, and B-cell lymphoma 2-like protein 4 (BAX), B-cell lymphoma 2 (Bcl-2), and p53 gene expression in the wound tissue were evaluated for 21 days. Antioxidant activity was further measured by 2,20-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and 2,2-Di(4-tert-octylphenyl)-1-picrylhydrazyl (DPPH) assays. Results: The animals in the treated groups showed higher wound contraction ratios (P ⟨ .05), angiogenesis, fibroblast, fibrocyte, and mast-cell distribution and intracytoplasmic carbohydrate storage compared with the control group (P ⟨ .05). Moreover, the topical administration of T repens increased the messenger ribonucleic acid (mRNA) level of Bcl-2 and reduced the BAX and p53 mRNA levels (P ⟨ .05). These findings further revealed the strong antioxidant activity of T repens. Conclusions: The topical administration of T repens accelerated wound healing by increasing angiogenesis; fibroblast, fibrocyte, and mast-cell distribution; intracytoplasmic carbohydrate storage; and modulation in genes involved in apoptosis in a rat model.

Comparisons of Floral Response of Seed Lots of Dollard Red Clover, Trifolium pratense L. 1

Crop Science ◽  
1965 ◽  
Vol 5 (5) ◽  
pp. 425-428 ◽  
Author(s):  
R. J. Bula ◽  
R. G. May ◽  
C. S. Garrison ◽  
C. M. Rincker ◽  
J. G. Dean
Keyword(s):  
Trifolium Pratense ◽  
Red Clover ◽  
Trifolium Pratense L

Plantago australis Hydroethanolic Extract-Loaded Formulations: Promising Dressings for Wound Healing

2021 ◽  
Vol 31 (1) ◽  
pp. 91-101
Author(s):  
Luiza Steffens Reinhardt ◽  
Jeferson Gustavo Henn ◽  
Ana Moira Morás ◽  
Nathalia Denise de Moura Sperotto ◽  
Matheus Bernardes Ferro ◽  
...  
Keyword(s):  
Wound Healing ◽  
Hydroethanolic Extract

scholarly journals Precrop Effect of Red Clover (Trifolium pratense L.) and Alfalfa (Medicago sativa L.) on the Population Development of the Northern Root-Knot Nematode Meloidogyne hapla Chitwood, 1949 and on Succeeding Crops—A Pot Study

Agronomy ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 722
Author(s):  
Renata Dobosz ◽  
Roman Krawczyk
Keyword(s):  
Population Density ◽  
Medicago Sativa ◽  
Trifolium Pratense ◽  
Red Clover ◽  
Continuous Cultivation ◽  
Nematode Population ◽  
Meloidogyne Hapla ◽  
Root Knot Nematode ◽  
Crop Species ◽  
Tomato Growth

The northern root-knot nematode, Meloidogyne hapla, is a major pest of many crop species. The objective of the study was to determine how M. hapla population dynamics is affected by two precrops, i.e., Trifolium pratense and Medicago sativa, in three crop durations: one, two and three years of continuous cultivation. Moreover, we set ourselves the task of evaluating the effect of the legume precrop soil on the growth of the succeeding tomato plant (Solanum lycopersicum) and on the nematode population. The experiment was performed outdoors in pots with naturally infected soil. Both precrop species investigated were found to modify the J2 nematode population density in the soil. The galls and nematode females with egg masses were observed on the roots of both studied plant species at the end of each growing season. They appeared to be more abundant on the red clover roots than on those of the alfalfa. The obtained data indicate that the spring soil sampling is more appropriate for the estimation of the M. hapla population density in the red clover precrop soil. The legume precrop soil had a limiting effect on tomato growth and fruit yield. The nematode population negatively influenced tomato growth. The experiment revealed that tomato plants could be planted in alfalfa precrop soil following at least three years of continuous alfalfa cultivation. The same cannot be said of the cultivation of red clover as a precrop for tomatoes.

scholarly journals Volatile Constituents of Trifolium Pratense and T. Repens from N.E. Italian Alpine Pastures

2009 ◽  
Vol 4 (6) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Aldo Tava ◽  
Daniele Ramella ◽  
Maris Grecchi ◽  
Paolo Aceto ◽  
Renato Paoletti ◽  
...  
Keyword(s):  
Steam Distillation ◽  
Trifolium Pratense ◽  
Red Clover ◽  
Volatile Oil ◽  
Volatile Fraction ◽  
Fresh Weight ◽  
Fresh Weight Basis ◽  
Qualitative And Quantitative ◽  
Aerial Parts ◽  
Trifolium Pratense L

The composition of the volatile fraction of two important forage legumes from Italian sub-alpine N.E. pastureland, namely Trifolium pratense L. subsp. pratense (red clover) and T. repens subsp. repens (white clover) were investigated. The volatile oil was obtained from the fresh aerial parts by steam distillation and analyzed by GC/FID and GC/MS. The oil yield was 0.018 and 0.021% (weight/fresh weight basis) for T. pratense and T. repens, respectively. Several classes of compounds were found in both the oils, including alcohols, aldehydes, ketones, terpenes, esters, hydrocarbons, phenolics and acids. Qualitative and quantitative differences were found.

scholarly journals The Effects of Red Clover Seed Treatment with Cold Plasma and Electromagnetic Field on Germination and Seedling Growth Are Dependent on Seed Color

2021 ◽  
Vol 11 (10) ◽  
pp. 4676
Author(s):  
Anatolii Ivankov ◽  
Rasa Zukiene ◽  
Zita Nauciene ◽  
Laima Degutyte-Fomins ◽  
Irina Filatova ◽  
...  
Keyword(s):  
Electromagnetic Field ◽  
Cold Plasma ◽  
Trifolium Pratense ◽  
Root Nodules ◽  
Germination Rate ◽  
Red Clover ◽  
Seed Color ◽  
Seed Treatments ◽  
Capacitively Coupled ◽  
Clover Seed

This study aimed to estimate the effects of cold plasma (CP) and electromagnetic field (EMF) treatment of red clover (Trifolium pratense) seeds with different coat colors on germination kinetics, the content of seed phytohormones, and the growth of seedlings. Seeds of red clover cultivar ‘Arimaiciai’ were treated with radio-frequency EMF or capacitively coupled low-pressure CP for different durations. There were no differences in germination kinetics between yellow, brown, and dark purple seeds in control, but the germination rate of seeds treated with CP and EMF depended on seed color: The germination of yellow seeds was stimulated stronger compared to dark purple and brown seeds, and EMF did not stimulate germination in brown seeds. The content of phytohormones in control seeds and the shift in their amount induced by seed treatments were also strongly dependent on seed color. No relationship was found between the effect on germination kinetics and changes in phytohormone levels. In the control, seedlings growing from the yellow seeds were heavier, and the number of root nodules was 12.5 times larger compared to seedlings of dark purple seeds. Seed treatments with CP and EMF significantly increased the number of root nodules, and this effect was stronger in seedlings from dark purple seeds compared to those from yellow seeds.

Sign in / Sign up

Export Citation Format

Share Document