Feedstock Carbon Influence on Compost Biochemical Stability and Maturity

2017 ◽  
Vol 26 (1) ◽  
pp. 59-70 ◽  
Author(s):  
Vidya de Gannes ◽  
Gaius Eudoxie ◽  
William J. Hickey
Keyword(s):  
Biochemical Stability

The ΔF508 mutation shortens the biochemical half-life of plasma membrane CFTR in polarized epithelial cells

2001 ◽  
Vol 280 (1) ◽  
pp. C166-C174 ◽  
Author(s):  
Ghanshyam D. Heda ◽  
Mridul Tanwani ◽  
Christopher R. Marino
Keyword(s):  
Plasma Membrane ◽  
Epithelial Cells ◽  
Cell Surface ◽  
Half Life ◽  
Immunoblot Analysis ◽  
Wild Type ◽  
Δf508 Cftr ◽  
Polarized Epithelial Cells ◽  
Physical And Chemical ◽  
Biochemical Stability

Although the biosynthetic arrest of the ΔF508 mutant of cystic fibrosis transmembrane conductance regulator (CFTR) can be partially reversed by physical and chemical means, recent evidence suggests that the functional stability of the mutant protein after reaching the cell surface is compromised. To understand the molecular basis for this observation, the current study directly measured the half-life of ΔF508 and wild-type CFTR at the cell surface of transfected LLC-PK1 cells. Plasma membrane CFTR expression over time was characterized biochemically and functionally in these polarized epithelial cells. Surface biotinylation, streptavidin extraction, and quantitative immunoblot analysis determined the biochemical half-life of plasma membrane ΔF508 CFTR to be ∼4 h, whereas the plasma membrane half-life of wild-type CFTR exceeded 48 h. This difference in biochemical stability correlated with CFTR-mediated transport function. These findings indicate that the ΔF508 mutation decreases the biochemical stability of CFTR at the cell surface. We conclude that the ΔF508 mutation triggers more rapid internalization of CFTR and/or its preferential sorting to a pathway of rapid degradation.

Rapid synthesis of Au/Ag bimetallic nanoclusters with highly biochemical stability and its applications for temperature and ratiometric pH sensing

2019 ◽  
Vol 1070 ◽  
pp. 88-96 ◽  
Author(s):  
Huanhuan Sun ◽  
Taiping Qing ◽  
Xiaoxiao He ◽  
Jingfang Shangguan ◽  
Ruichen Jia ◽  
...  
Keyword(s):  
Ph Sensing ◽  
Rapid Synthesis ◽  
Bimetallic Nanoclusters ◽  
Ratiometric Ph Sensing ◽  
Biochemical Stability

Biochemical Stability of Components for Use in a DNA Detection System

2004 ◽  
Vol 40 (4) ◽  
pp. 3012-3014 ◽  
Author(s):  
V. Joshi ◽  
G. Li ◽  
S.X. Wang ◽  
S. Sun
Keyword(s):  
Detection System ◽  
Dna Detection ◽  
Biochemical Stability
Sign in / Sign up

Export Citation Format

Share Document