Free radicals in exhaled breath condensate in cystic fibrosis and healthy subjects

2006 ◽  
Vol 40 (9) ◽  
pp. 901-909 ◽  
Author(s):  
Philippe P.R. Rosias ◽  
Gertjan J.M. Den Hartog ◽  
Charlotte M.H.H.T. Robroeks ◽  
AALT Bast ◽  
Raymond A.M.G. Donckerwolcke ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Free Radicals ◽  
Healthy Subjects ◽  
Exhaled Breath Condensate ◽  
Exhaled Breath ◽  
Breath Condensate

scholarly journals Exhaled Breath Condensate Detects Baseline Reductions in Chloride and Increases in Response to Albuterol in Cystic Fibrosis Patients

2013 ◽  
Vol 7 ◽  
pp. CCRPM.S12882 ◽  
Author(s):  
Courtney M. Wheatley ◽  
Wayne J. Morgan ◽  
Nicholas A. Cassuto ◽  
William T. Foxx-Lupo ◽  
Cori L. Daines ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Exhaled Breath Condensate ◽  
Ionic Composition ◽  
Membrane Conductance ◽  
Pharmacological Therapy ◽  
Exhaled Breath ◽  
Ion Regulation ◽  
Capillary Blood Volume ◽  
Pulmonary Capillary Blood ◽  
Breath Condensate

Impaired ion regulation and dehydration is the primary pathophysiology in cystic fibrosis (CF) lung disease. A potential application of exhaled breath condensate (EBC) collection is to assess airway surface liquid ionic composition at baseline and in response to pharmacological therapy in CF. Our aims were to determine if EBC could detect differences in ion regulation between CF and healthy and measure the effect of the albuterol on EBC ions in these populations. Baseline EBC Cl−, DLCO and SpO2 were lower in CF (n = 16) compared to healthy participants (n = 16). EBC Cl− increased in CF subjects, while there was no change in DLCO or membrane conductance, but a decrease in pulmonary-capillary blood volume in both groups following albuterol. This resulted in an improvement in diffusion at the alveolar-capillary unit, and removal of the baseline difference in SpO2 by 90-minutes in CF subjects. These results demonstrate that EBC detects differences in ion regulation between healthy and CF individuals, and that albuterol mediates increases in Cl− in CF, suggesting that the benefits of albuterol extend beyond simple bronchodilation.

scholarly journals Exhaled Breath Condensate: Pilot Study of the Method and Initial Experience in Healthy Subjects

2018 ◽  
Vol 61 (1) ◽  
pp. 8-16
Author(s):  
Eva Peterová ◽  
Jaroslav Chládek ◽  
Darina Kohoutová ◽  
Veronika Knoblochová ◽  
Paula Morávková ◽  
...  
Keyword(s):  
Pilot Study ◽  
Healthy Volunteers ◽  
Coefficient Of Variation ◽  
Healthy Subjects ◽  
Lung Diseases ◽  
Exhaled Breath Condensate ◽  
Exhaled Breath ◽  
Initial Experience ◽  
Major Disadvantage ◽  
Breath Condensate

Analysis of Exhaled breath condensate (EBC) is a re-discovered approach to monitoring the course of the disease and reduce invasive methods of patient investigation. However, the major disadvantage and shortcoming of the EBC is lack of reliable and reproducible standardization of the method. Despite many articles published on EBC, until now there is no clear consensus on whether the analysis of EBC can provide a clue to diagnosis of the diseases. The purpose of this paper is to investigate our own method, to search for possible standardization and to obtain our own initial experience. Thirty healthy volunteers provided the EBC, in which we monitored the density, pH, protein, chloride and urea concentration. Our results show that EBC pH is influenced by smoking, and urea concentrations are affected by the gender of subjects. Age of subjects does not play a role. The smallest coefficient of variation between individual volunteers is for density determination. Current limitations of EBC measurements are the low concentration of many biomarkers. Standardization needs to be specific for each individual biomarker, with focusing on optimal condensate collection. EBC analysis has a potential become diagnostic test, not only for lung diseases.

Feasibility of Early Detection of Cystic Fibrosis Acute Pulmonary Exacerbations by Exhaled Breath Condensate Metabolomics: A Pilot Study

2016 ◽  
Vol 16 (2) ◽  
pp. 550-558 ◽  
Author(s):  
Xiaoling Zang ◽  
María Eugenia Monge ◽  
Nael A. McCarty ◽  
Arlene A. Stecenko ◽  
Facundo M. Fernández
Keyword(s):  
Cystic Fibrosis ◽  
Pilot Study ◽  
Early Detection ◽  
Exhaled Breath Condensate ◽  
Exhaled Breath ◽  
Pulmonary Exacerbations ◽  
Breath Condensate

MicroRNA-26a expression in exhaled breath condensate correlates with pulmonary exacerbation in cystic fibrosis

2020 ◽  
Author(s):  
Zuzanna Stachowiak ◽  
Irena Wojsyk-Banaszak ◽  
Katarzyna Jończyk-Potoczna ◽  
Joanna Goździk-Spychalska ◽  
Kamila Sobczak ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Exhaled Breath Condensate ◽  
Exhaled Breath ◽  
Pulmonary Exacerbation ◽  
Breath Condensate

scholarly journals Increased nitrotyrosine in exhaled breath condensate in cystic fibrosis

2001 ◽  
Vol 17 (6) ◽  
pp. 1201-1207 ◽  
Author(s):  
B. Balint ◽  
S.A. Kharitonov ◽  
T. Hanazawa ◽  
L.E. Donnelly ◽  
P.L. Shah ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Exhaled Breath Condensate ◽  
Exhaled Breath ◽  
Breath Condensate

Exhaled Inflammatory Markers in Aspirin-Induced Asthma Syndrome

2007 ◽  
Vol 21 (5) ◽  
pp. 542-547 ◽  
Author(s):  
Giovanna E. Carpagnano ◽  
Onofrio Resta ◽  
Matteo Gelardi ◽  
Antonio Spanevello ◽  
Giuseppe Di Gioia ◽  
...  
Keyword(s):  
Inflammatory Markers ◽  
Healthy Subjects ◽  
Nasal Polyposis ◽  
Exhaled Breath Condensate ◽  
Mild Asthma ◽  
Exhaled Breath ◽  
Eosinophilic Inflammation ◽  
Pathological Conditions ◽  
Lower Airways ◽  
Breath Condensate

Background Interleukin (IL)-4 and IL-6, respectively, markers of neutrophilic and eosinophilic inflammation, were analyzed in nasal and oral exhaled breath condensate to understand the inflammation of upper and lower airways in subjects with aspirin-induced asthma (AIA) syndrome, evaluating possible differences between AIA and the single pathological conditions included in AIA syndrome. Methods Twelve patients with AIA, 17 patients with mild asthma (MA), 12 patients with nasal polyposis (NP), 11 patients with mild asthma + nasal polyposis (MA + NP), and 10 healthy subjects (HSs) were enrolled. Nasal and oral exhaled IL-4 and IL-6 were measured by enzyme immunoassay kit. Results Higher levels of nasal and oral exhaled IL-4 and IL-6 were observed in AIA compared with MA, NP, MA + NP, and HSs. Moreover, a positive correlation was identified between nasal exhaled IL-4 and IL-6 and, respectively, the number of neutrophils and eosinophils and in nasal scraping. Conclusion The concentration of eosinophilic and neutrophilic markers in upper and lower airways of subjects with AIA syndrome is higher compared with HS and subjects with MA, NP, and MA + NP.

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