An approach based on consecutive high-speed counter-current chromatography for preparation of an active compound rutin from Apocynum venetum L

2021 ◽  
pp. 1-8
Author(s):  
Haoquan Li ◽  
Zengyuan Cao ◽  
Shuang Guo ◽  
Dongyu Gu ◽  
Shuai Wang ◽  
...  
Keyword(s):  
Active Compound ◽  
High Speed ◽  
Apocynum Venetum ◽  
Counter Current

scholarly journals Counter-Current Fractionation-Assisted Bioassay-Guided Separation of Active Compound from Blueberry and the Interaction between the Active Compound and α-Glucosidase

Foods ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 509
Author(s):  
Hongkun Xue ◽  
Xiaohan Zhu ◽  
Jiaqi Tan ◽  
Linlin Fan ◽  
Qian Li ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Hydrogen Bonds ◽  
Active Compound ◽  
High Speed ◽  
High Performance ◽  
Interaction Mechanism ◽  
Water Extract ◽  
Active Components ◽  
Fourier Transformed Infrared Spectroscopy ◽  
Counter Current

An efficient strategy for the selection of active compounds from blueberry based on counter-current fractionation and bioassay-guided separation was established in this study. Blueberry extract showed potential α-glucosidase inhibitory activity. After extraction by different solvents, the active components were enriched in water. The water extract was divided into six fractions via high-speed counter-current chromatography to further track the active components. Results indicated that the α-glucosidase inhibition rate of F4 was remarkable higher than the others. Cyanidin-3-glucoside (C3G) with a purity of 94.16% was successfully separated from F4 through column chromatography, and its structure was identified by ultraviolet spectral, Fourier-transformed infrared spectroscopy, high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry, 1H nuclear magnetic resonance (NMR), and 13C NMR. The interaction mechanism between C3G and α-glucosidase was clearly characterized and described by spectroscopic methods, including fluorescence and circular dichroism (CD) in combination with molecular docking techniques. C3G could spontaneously bind with α-glucosidase to form complexes by hydrogen bonds. The secondary structure of α-glucosidase changed in varying degrees after complexation with C3G. The α-helical and β-turn contents of α-glucosidase decreased, whereas the β-sheet content and the irregular coil structures increased. Molecular docking speculated that C3G could form hydrogen bonds with α-glucosidase by binding to the active sit (Leu 313, Ser 157, Tyr 158, Phe 314, Arg 315, and two Asp 307). These findings may be useful for the development of functional foods to tackle type 2 diabetes.

Solvent gradient elution for comprehensive separation of constituents with wide range of polarity in Apocynum venetum leaves by high-speed counter-current chromatography

2010 ◽  
Vol 33 (17-18) ◽  
pp. 2743-2748 ◽  
Author(s):  
Yuchi Zhang ◽  
Chunming Liu ◽  
Zhengkun Zhang ◽  
Yanjuan Qi ◽  
Guimei Wu ◽  
...  
Keyword(s):  
High Speed ◽  
Gradient Elution ◽  
Wide Range ◽  
Apocynum Venetum ◽  
Counter Current

Separation and preparation of two rotenoids from the roots of Derris by high-speed counter-current chromatography

2010 ◽  
Vol 28 (8) ◽  
pp. 813-816
Author(s):  
Wei JI ◽  
Yulan CUI ◽  
Renjuan CHEN ◽  
Xinren LIN ◽  
Qingxin ZHOU ◽  
...  
Keyword(s):  
High Speed ◽  
Counter Current

Isolation and purification of flavones from Murraya exotica L. by high-speed counter-current chromatography

2010 ◽  
Vol 28 (4) ◽  
pp. 383-387 ◽  
Author(s):  
Aiyi PENG ◽  
Xuewei QU ◽  
Hui LI ◽  
Lu GAO ◽  
Bo YU ◽  
...  
Keyword(s):  
High Speed ◽  
Isolation And Purification ◽  
Murraya Exotica ◽  
Counter Current

One-step Separation and Purification of Four Phenolic Acids from Stenoloma chusanum (L.) Ching by Medium-pressure Liquid Chromatography and High-speed Counter-current Chromatography

2019 ◽  
Vol 9 (2) ◽  
pp. 138-143
Author(s):  
Tianyun Li ◽  
Xiling Dai ◽  
Yichen Li ◽  
Guozheng Huang ◽  
Jianguo Cao
Keyword(s):  
Natural Products ◽  
Liquid Chromatography ◽  
Phenolic Acids ◽  
High Speed ◽  
High Efficiency ◽  
Total Phenolic ◽  
Medium Pressure ◽  
Medium Pressure Liquid Chromatography ◽  
One Step ◽  
Counter Current

Background:Stenoloma chusanum (L.) Ching is a Chinese traditional medicinal fern with high total flavonoid and total phenolic content. Traditionally, phenolic compounds were separated by using column chromatography, which is relatively inefficient. </P><P> Objective: This study aims to use an efficient method to separate natural products from S. chusanum by Medium-Pressure Liquid Chromatography (MPLC) and High-Speed Counter-Current Chromatography (HSCCC).Methods:In the present research, firstly, a sample (2.5 g) from the dichloromethane extract of S. chusanum was separated by MPLC. Next, fraction P5 was purified by HSCCC with a two-phase solvent system composed of hexane-ethyl acetate-methanol-water (HEMWat) at a volume ratio of 2:4:1:4 (v/v/v/v). </P><P> Result: Four phenolic acids were obtained and their structures were identified by means of NMR and ESI-mass analysis. They were identified as: 1) protocatechuic acid (34 mg, purity 90.1%), 2) syringic acid (66 mg, purity 99.0%), 3) p-hydroxybenzoic acid (5 mg, purity 91.2%) and 4) vanillic acid (6 mg, purity 99.3%).Conclusion:The combination of MPLC and HSCCC is a high-efficiency separation method for natural products. This is the first report with regard to the separation of four phenolic acids in one step by MPLC and HSCCC from S. chusanum (L.) Ching.

HSCCC Separation of Three Main Compounds from the Crude Extract of Dracocephalum Tanguticum by Using Dimethyl Sulfoxide as Cosolvent

2020 ◽  
Author(s):  
Xue Yang ◽  
Yongling Liu ◽  
Tao Chen ◽  
Nana Wang ◽  
Hongmei Li ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Ethyl Acetate ◽  
Dimethyl Sulfoxide ◽  
Efficient Method ◽  
Crude Extract ◽  
High Speed ◽  
Glacial Acetic Acid ◽  
Butyl Alcohol ◽  
Preparative Hplc ◽  
Counter Current

Abstract Separation of natural compounds directly from the crude extract is a challenging work for traditional column chromatography. In the present study, an efficient method for separation of three main compounds from the crude extract of Dracocephalum tanguticum has been successfully established by high-speed counter-current chromatography (HSCCC). The crude extract was directly introduced into HSCCC by using dimethyl sulfoxide as cosolvent. Ethyl acetate/n-butyl alcohol/0.3% glacial acetic acid (4: 1: 5, v/v) system was used and three target compounds with purity higher than 80% were obtained. Preparative HPLC was used for further purification and three target compounds with purity higher than 98% were obtained. The compounds were identified as chlorogenic acid, pedaliin and pedaliin-6″-acetate.

scholarly journals Scale-Up Preparation of Crocins I and II from Gardeniajasminoides by a Two-Step Chromatographic Approach and Their Inhibitory Activity Against ATP Citrate Lyase

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3137
Author(s):  
Shuguang Guan ◽  
Qiaoli Pu ◽  
Yinan Liu ◽  
Honghong Wu ◽  
Wenbo Yu ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
High Speed ◽  
Scale Up ◽  
Cost Effective ◽  
Atp Citrate Lyase ◽  
Gardenia Jasminoides ◽  
Citrate Lyase ◽  
Human Disorders ◽  
Ic50 Values ◽  
Counter Current

Crocins are highly valuable natural compounds for treating human disorders, and they are also high-end spices and colorants in the food industry. Due to the limitation of obtaining this type of highly polar compound, the commercial prices of crocins I and II are expensive. In this study, macroporous resin column chromatography combined with high-speed counter-current chromatography (HSCCC) was used to purify crocins I and II from natural sources. With only two chromatographic steps, both compounds were simultaneously isolated from the dry fruit of Gardenia jasminoides, which is a cheap herbal medicine distributed in a number of countries. In an effort to shorten the isolation time and reduce solvent usage, forward and reverse rotations were successively utilized in the HSCCC isolation procedure. Crocins I and II were simultaneously obtained from a herbal resource with high recoveries of 0.5% and 0.1%, respectively, and high purities of 98.7% and 99.1%, respectively, by HPLC analysis. The optimized preparation method was proven to be highly efficient, convenient, and cost-effective. Crocins I and II exhibited inhibitory activity against ATP citrate lyase, and their IC50 values were determined to be 36.3 ± 6.24 and 29.7 ± 7.41 μM, respectively.

Sign in / Sign up

Export Citation Format

Share Document