Early fish domestication affects methylation of key genes involved in the rapid onset of the farmed phenotype

SummaryADP-induced platelet aggregation and calcium-induced platelet aggregation tests were studied in 14 diabetic patients in the fasting state and half an hour after an intravenous injection of 0.1 unit insulin/kg body weight. Platelet disaggregation was significantly diminished as compared to a normal control group, and their results were negatively correlated with the corresponding serum cholesterol levels. Insulin caused significant diminution in the ADP-induced platelet aggregation as a result of rapid onset of aggregation and disaggregation. There was also a significant increase in platelet disaggregation. In the calcium-induced platelet aggregation test, there was a significant shortening of the aggregation time, its duration, and the clotting time. The optical density fall due to platelet aggregation showed a significant increase. Insulin may have a role in correcting platelet disaggregation possibly through improvement in the intracellular enzymatic activity.

Download Full-text

Assessing Acute Decompensated Heart Failure – Strategies and Tools

European Cardiology Review ◽

10.15420/ecr.2012.8.2.128 ◽

2012 ◽

Vol 8 (2) ◽

pp. 128

Author(s):

Ali Vazir ◽

Martin R Cowie ◽

◽

Keyword(s):

Heart Failure ◽

Acute Decompensated Heart Failure ◽

Pulse Contour Analysis ◽

Decompensated Heart Failure ◽

Rapid Onset ◽

Clinical Syndrome ◽

Response To Therapy ◽

Contour Analysis ◽

Physiological Variables ◽

Co Morbidity

Acute heart failure – the rapid onset of, or change in, signs and/or symptoms of heart failure requiring urgent treatment – is a serious clinical syndrome, associated with high mortality and healthcare costs. History, physical examination and early 2D and Doppler echocardiography are crucial to the proper assessment of patients, and will help determine the appropriate monitoring and management strategy. Most patients are elderly and have considerable co-morbidity. Clinical assessment is key to monitoring progress, but a number of clinical techniques – including simple Doppler and echocardiographic tools, pulse contour analysis and impedance cardiography – can help assess the response to therapy. A pulmonary artery catheter is not a routine monitoring tool, but can be very useful in patients with complex physiology, in those who fail to respond to therapy as would be anticipated, or in those being considered for mechanical intervention. As yet, the serial measurement of plasma natriuretic peptides is of limited value, but it does have a role in diagnosis and prognostication. Increasingly, the remote monitoring of physiological variables by completely implanted devices is possible, but the place of such technology in clinical practice is yet to be clearly established.

Download Full-text

Basic Andragogy-Oriented Strategies and Communication in Online Education

International conference KNOWLEDGE-BASED ORGANIZATION ◽

10.2478/kbo-2020-0103 ◽

2020 ◽

Vol 26 (2) ◽

pp. 350-356

Author(s):

Anca Sîrbu

Keyword(s):

Adult Learners ◽

Online Education ◽

Social Status ◽

Rapid Onset ◽

Learning Goals ◽

The Novel ◽

Academic Education ◽

Online Instructors ◽

New Methods ◽

The World

AbstractWith the rapid onset of an unprecedented lifestyle due to the new coronavirus COVID-19 the world academic scene was forced to reform and adapt to the novel circumstances. Although online education cannot be regarded as a groundbreaking endeavour anymore in the21st century, its current character of exclusivity calls for deeper understanding of, and a sharper focus on the “end-consumer” thereof as well as more cautious procedures to be exercised while teaching. While millennials are no longer thought of as being born with a silver spoon in their mouth but with an iPad or any sort of device in their hand (irrespective of their social status), adults are more hesitant when coerced to alter course unexpectedly and turn to new methods of attaining their learning goals. This is why proper communicative approaches need to be thoroughly considered by online instructors. This article aims at presenting teachers with a set of strategies to employ when the beneficiaries of online academic education are adult learners.

Download Full-text

Effect of cortisol on neurophysin I/oxytocin and peptidyl glycine-α-amidating mono-oxygenase mRNA expression in bovine luteal and granulosa cells

Polish Journal of Veterinary Sciences ◽

10.2478/pjvs-2013-0033 ◽

2013 ◽

Vol 16 (2) ◽

pp. 231-239

Author(s):

A. Ziolkowska ◽

J. Mlynarczuk ◽

J. Kotwica

Keyword(s):

Mrna Expression ◽

Granulosa Cells ◽

Molecular Mechanisms ◽

Hormone Secretion ◽

Oestrous Cycle ◽

Luteal Cells ◽

Ru 486 ◽

Ovarian Cells ◽

Key Genes ◽

Ovary Function

Abstract Cortisol stimulates the synthesis and secretion of oxytocin (OT) from bovine granulosa and luteal cells, but the molecular mechanisms of cortisol action remain unknown. In this study, granulosa cells or luteal cells from days 1-5 and 11-15 of the oestrous cycle were incubated for 4 or 8 h with cortisol (1x10-5, 1x10-7 M). After testing cell viability and hormone secretion (OT, progesterone, estradiol), we studied the effect of cortisol on mRNA expression for precursor of OT (NP-I/OT) and peptidyl glycine-α-amidating mono-oxygenase (PGA). The influence of RU 486 (1x10-5 M), a progesterone receptor blocker and inhibitor of the glucocorticosteroid receptor (GR), on the expression for both genes was tested. Cortisol increased the mRNA expression for NP-I/OT and PGA in granulosa cells and stimulated the expression for NP-I/OT mRNA in luteal cells obtained from days 1-5 and days 11-15 of the oestrous cycle. Expression for PGA mRNA was increased only in luteal cells from days 11-15 of the oestrous cycle. In addition, RU 486 blocked the cortisol-stimulated mRNA expression for NP-I/OT and PGA in both types of cells. These data suggest that cortisol affects OT synthesis and secretion in bovine ovarian cells, by acting on the expression of key genes, that may impair ovary function.

Download Full-text

Do Second-Time Users of Topical Imiquimod have a More Rapid Onset of Clinical Response?

SKIN The Journal of Cutaneous Medicine ◽

10.25251/skin.2.3.1 ◽

2018 ◽

Vol 2 (3) ◽

pp. 156-161

Author(s):

Stacy L McMurray ◽

Matthew Reynolds ◽

Matthew S Dinehart ◽

Scott M Dinehart

Keyword(s):

Clinical Response ◽

Time Use ◽

Γδ T Cells ◽

Rapid Onset ◽

Primary Objective ◽

Imiquimod Cream ◽

Actinic Keratoses ◽

Topical Imiquimod ◽

Primary Endpoints ◽

Time To Onset

Introduction: Topical imiquimod is commonly used in dermatology for treatment of actinic keratoses (AK). Prior studies in humans and mice have suggested the potential for immune recall with imiquimod based on higher degrees of AK clearance and activation of memory γδ T-cells in a mouse model. Anecdotal reports suggest a more rapid time-to-onset of clinical response with second time use of imiquimod. However, the potential for immune recall demonstrated by time-to-onset of clinical response has not been formally investigated.Objective: The primary objective of this study was to determine if there is a difference in time-to-onset of clinical response between naïve and prior users of topical imiquimod for the treatment of actinic keratoses.Methods: A total of 92 patients were treated with 5% imiquimod cream for actinic keratoses of the head and neck. Patients were instructed to apply 5% imiquimod cream to the affected areas once daily until reaching a therapeutic endpoint of crusting/scabbing. The primary endpoints in the study were time (days) to onset of erythema and time to onset of crusting/scabbing. Results were self-reported.Results: The average time (days) to onset of erythema was 5.48 ± 3.19 days in naïve users and 4.7 ± 2.91 days in prior users (p= 0.22). Average time to onset of crusting/scabbing was 9.2 ± 4.34 days in naïve users and 9.02 ± 3.65 days in prior users (p=0.35).Conclusion: Our study revealed there is no difference in time-to-onset of erythema or scabbing/crusting with second-time use of imiquimod. While immune recall may be possible with use of imiquimod, the results of this study indicate that it may be independent of time-to-onset of clinical response.

Download Full-text