Tannin-degrading bacteria with cellulase activity isolated from the cecum of the Qinghai-Tibet plateau zokor (Myospalax baileyi)

2015 ◽  
Vol 61 (1) ◽  
pp. 21-27 ◽  
Author(s):  
Anatoly Bobrov ◽  
Vasiliy D. Kravchenko ◽  
Günter C. Müller
Keyword(s):  
Cellulase Activity ◽  
Tibet Plateau ◽  
Bacterial Strains ◽  
Negative Effects ◽  
Hydrolyzable Tannin ◽  
16S Rdna Gene ◽  
Qinghai Province ◽  
Degrading Bacteria ◽  
Qinghai Tibet Plateau ◽  
Plateau Zokor

Tannins, which are polyphenols present in various plants, have anti-nutritional activity; however, their negative effects are mitigated by the presence of tannin-degrading microorganisms in the gastrointestinal tract of animals. This has never been investigated in the plateau zokor (Myospalax baileyi) – the predominant small herbivore in the alpine meadow ecosystem of Qinghai Province, China – which consumes tannin-rich herbaceous plants. Tannase activity in the feces of the plateau zokor increased from June to August corresponding to the increase in hydrolyzable tannin concentrations in plants during this period, and three tannin-degrading facultative anaerobic strains (designated as E1, E2, and E3) were isolated from the cecum of these animals. Sequencing of the 16S rDNA gene identified isolates of strain E1 as belonging to the genusEnterococcus, and E2 and E3 to the genusBacillus. All of the bacteria had cellulose-degrading capacity. This study provides the first evidence of symbiotic bacterial strains that degrade tannic acid and cellulose in the cecum of plateau zokor.

Gut microbiome adaptation to extreme cold winter in wild plateau pika (Ochotona curzoniae) on the Qinghai-Tibet Plateau

2020 ◽  
Vol 367 (16) ◽  
Author(s):  
Yijie Wang ◽  
Rui Zhou ◽  
Qiaoling Yu ◽  
Tianshu Feng ◽  
Huan Li
Keyword(s):  
High Altitude ◽  
Gut Microbiome ◽  
Cellulase Activity ◽  
Short Chain Fatty Acids ◽  
Tibet Plateau ◽  
Plateau Pika ◽  
Ochotona Curzoniae ◽  
Degrading Bacteria ◽  
Extreme Cold ◽  
Qinghai Tibet Plateau

ABSTRACT The Qinghai-Tibet Plateau is a harsh environment characterized by low temperature, high altitude and hypoxia, although some native mammals may adapt well to the extreme climate. However, how animal gut microbial community structure and function adapt to extreme cold climates is not well understood. Plateau pika (Ochotona curzoniae) is an ideal animal model with which to study the effects of climate change on host adaptation by studing intestinal microorganisms. Here, we used 16S rRNA sequencing technology combined with physiological methods to investigate plateau pika gut microbiota in summer and winter. Due to limited diet resources, the pikas in winter have a lower ability of degradation and fermentation for plant-based food (reduced cellulase activity and total short-chain fatty acids) by decreasing gut microbial diversity and some functional microbes, such as fiber-degrading bacteria Oscillospira and Treponema. Metagenomic prediction showed that most of those gene functions associated with metabolism (e.g. energy metabolism and lipid metabolism) were less abundant in winter, implying that the plateau pika slows diet fermentation and weakens energy requirements in the cold season. Our results have significance for explaining the mechanism of wild plateau mammals adapting to a high-altitude cold environment from the perspective of gut microbiome.

Actinomyces marmotae sp. nov. and Actinomyces procaprae sp. nov. isolated from wild animals and reclassification of Actinomyces liubingyangii and Actinomyces tangfeifanii as Boudabousia liubingyangii comb. nov. and Boudabousia tangfeifanii comb. nov., respectively

2019 ◽  
Vol 71 (3) ◽  
Author(s):  
Caixin Yang ◽  
Yibo Bai ◽  
Kui Dong ◽  
Jing Yang ◽  
Xin-He Lai ◽  
...  
Keyword(s):  
Type Species ◽  
Phylogenetic Analyses ◽  
Brain Heart Infusion ◽  
Tibet Plateau ◽  
Rrna Gene ◽  
Bacterial Strains ◽  
Content Type ◽  
Link Type ◽  
Pr China ◽  
Qinghai Tibet Plateau

Four Gram-stain-positive, catalase-negative, non-spore-forming, rod-shaped bacterial strains (zg-325T, zg329, dk561T and dk752) were isolated from the respiratory tract of marmot (Marmota himalayana) and the faeces of Tibetan gazelle (Procapra picticaudata) from the Qinghai-Tibet Plateau of PR China. The results of 16S rRNA gene sequence-based phylogenetic analyses indicated that strains zg-325T and dk561T represent members of the genus Actinomyces , most similar to Actinomyces denticolens DSM 20671T and Actinomyces ruminicola B71T, respectively. The DNA G+C contents of strains zg-325T and dk561T were 71.6 and 69.3 mol%, respectively. The digital DNA–DNA hybridization values of strains zg-325T and dk561T with their most closely related species were below the 70 % threshold for species demarcation. The four strains grew best at 35 °C in air containing 5 % CO2 on brain heart infusion (BHI) agar with 5 % sheep blood. All four strains had C18:1ω9c and C16:0 as the major cellular fatty acids. MK-8 and MK-9 were the major menaquinones in zg-325T while MK-10 was predominant in dk561T. The major polar lipids included diphosphatidylglycerol and phosphatidylinositol. On the basis of several lines of evidence from phenotypic and phylogenetic analyses, zg-325T and dk561T represent novel species of the genus Actinomyces , for which the name Actinomyces marmotae sp. nov. and Actinomyces procaprae sp. nov. are proposed. The type strains are zg-325T (=GDMCC 1.1724T=JCM 34091T) and dk561T (=CGMCC 4.7566T=JCM 33484T). We also propose, on the basis of the phylogenetic results herein, the reclassification of Actinomyces liubingyangii and Actinomyces tangfeifanii as Boudabousia liubingyangii comb. nov. and Boudabousia tangfeifanii comb. nov., respectively.

Study on Greenhouse Gas Emissions from the Mounds Produced by Plateau Zokor (Eospalax Baileyi) on Qinghai–Tibet Plateau

2021 ◽  
Vol 78 ◽  
pp. 36-45
Author(s):  
Zhuangsheng Tang ◽  
Yongmei Jiang ◽  
Rui Hua ◽  
Jianwei Zhou ◽  
Bin Chu ◽  
...  
Keyword(s):  
Greenhouse Gas Emissions ◽  
Greenhouse Gas ◽  
Tibet Plateau ◽  
Gas Emissions ◽  
Qinghai Tibet Plateau ◽  
Plateau Zokor

scholarly journals Salinibacterium hongtaonis sp. nov., isolated from faeces of Tibetan antelope (Pantholops hodgsonii) on the Qinghai–Tibet Plateau

2019 ◽  
Vol 69 (4) ◽  
pp. 1093-1098 ◽  
Author(s):  
Junqin Li ◽  
Shan Lu ◽  
Dong Jin ◽  
Jing Yang ◽  
Xin-He Lai ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Tibet Plateau ◽  
Rrna Gene ◽  
Bacterial Strains ◽  
Gene Sequences ◽  
16S Rrna Gene Sequences ◽  
Content Type ◽  
Link Type ◽  
Qinghai Tibet Plateau

Two novel aerobic, Gram-staining-positive and non-spore-forming bacterial strains, 194T and S1194, were isolated from faeces of Tibetan antelopes sampled at the Qinghai–Tibet Plateau of China. The strains were able to grow in medium up to 10 % NaCl, similar to the NaCl-resistant property of the genus Salinibacterium members. The 16S rRNA gene sequences of the strains showed the highest similarity to Salinibacterium xinjiangense(98.1–98.2 %), and phylogenetic analysis based on 16S rRNA gene sequences indicated that strains 194T and S1194 represent a new lineage. The DNA G+C contents of strain 194T and S1194 are 64.1 and 64.2 mol%. Their genomes exhibit less than 96 % average nucleotide identity and 70 % DNA–DNA relatedness to known species of Salinibacterium . Strains 194T and S1194 are unable to utilize d-mannose or produce naphthol-AS-BI-phosphohydrolase. The two strains had anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids, and their cell walls contained lysine, alanine, glycine and glutamic acid. The predominant menaquinones identified were MK-11 and MK-10, with diphosphatidylglycerol and phosphatidylglycerol as major polar lipids. Overall, the major cellular content profiles of 194T agreed with those of Salinibacterium xinjiangense and Salinibacterium amurskyense, though the proportions were distinct. Based on genotypic, phenotypic and biochemical analyses, the novel species Salinibacterium hongtaonis sp. nov. is proposed. The type strain is 194T (=CGMCC 1.16371T=DSM 106171T).

scholarly journals Report on the 19th international symposium on geo-disaster reduction/high-level academic forum on disaster mitigation and integrated risk defense on the Plateau, 12–15 July 2021, Xining, China

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Fenggui Liu ◽  
Guolong Zhu ◽  
Fawu Wang
Keyword(s):  
International Symposium ◽  
Disaster Mitigation ◽  
Tibet Plateau ◽  
Research Progress ◽  
Disaster Reduction ◽  
Qinghai Province ◽  
Integrated Risk ◽  
Joint Event ◽  
High Level ◽  
Qinghai Tibet Plateau

AbstractThe joint event of 19th International Symposium on Geo-disaster Reduction (19ISGdR) and High-Level Academic Forum on Disaster Mitigation and Integrated Risk Defense on the Plateau was held on 11–15 July in Xining, Qinghai Province, China, focusing on the theme of “Geological disaster and integrated risk defense”. This event consisted of keynote lectures, invited lectures, and Youth forum, which provided a platform for scientists, industrial professionals and young scholars to share their research progress and exchange novel ideas on geo-disaster reduction in a hybrid way of offline and online. A post-symposium field trip for three days was also conducted in the joint area between Qinghai-Tibet plateau and Loess plateau.

scholarly journals Emergy-Based Evaluation of Changes in Agrochemical Residues on the Qinghai–Tibet Plateau, China

2019 ◽  
Vol 11 (13) ◽  
pp. 3652 ◽  
Author(s):  
Xiuhong Wang ◽  
Yili Zhang
Keyword(s):  
Intensity Factor ◽  
Structure Factor ◽  
Synthesis Method ◽  
Tibet Plateau ◽  
Annual Growth Rate ◽  
Qinghai Province ◽  
Productivity Factor ◽  
Tibet Autonomous Region ◽  
Agricultural Materials ◽  
Qinghai Tibet Plateau

Study of changes in agrochemical residues on the Qinghai–Tibet Plateau is necessary for the agricultural green development of the fragile plateau and its downstream regions. The total agrochemical residue (TR) caused by main agrochemical inputs was estimated in the study area of Qinghai province and the Tibet Autonomous Region over 1995–2017 by using the emergy synthesis method. The total agrochemical residue was decomposed into the intensity factor, the structure factor, the productivity factor, and the labour factor by using the Logarithmic Mean Divisia Index (LMDI) decomposition method. The change in TR could be divided into four time periods, i.e., a rapidly increasing period during 1995–1998, a stable period during 1999–2004, a slowly increasing period during 2005–2011, and a fluctuant period during 2012–2017. The study area had a mean TR intensity in area (TRA) of 3.31 × 1014 sej/ha, which was only 38.21% of that in China; however, the annual growth rate of TRA in the study area was 2.93%, higher than the rate of 1.91% in China over 1995–2017. The study area had a mean TR intensity in production (TRP) of 4.06 × 1010 sej/CNY (Chinese Yuan), which was 71.05% of that in China; however, the annual decreasing rate of TRP in the study area was 0.95%, lower than the rate of 1.98% in China over 1995–2017. All the LMDI decomposed factors contributed to the TR increase during 1995–1998; the intensity factor, the structure factor, and the labour factor contributed to the TR decrease during 1999–2004; the structure factor and the productivity factor contributed to the TR increase during 2005–2011; and only the productivity factor contributed to the TR increase during 2012–2017. Compared with the whole country, the study area has more potential to reduce TR by improving agrochemical use efficiency, strengthening the recovery of plastic film residue, increasing organic agricultural materials, raising the efficiency of agricultural production, and accelerating the transfer of rural labours to secondary and tertiary industries.

scholarly journals Optimizing livestock carrying capacity for wild ungulate-livestock coexistence in a Qinghai-Tibet Plateau grassland

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yueheng Ren ◽  
Yanpeng Zhu ◽  
Davide Baldan ◽  
Mengdi Fu ◽  
Bin Wang ◽  
...  
Keyword(s):  
Carrying Capacity ◽  
Critical Role ◽  
Terrestrial Ecosystems ◽  
Livestock Grazing ◽  
Tibet Plateau ◽  
Wild Ungulates ◽  
Livestock Farming ◽  
Qinghai Province ◽  
Key Species ◽  
Qinghai Tibet Plateau

AbstractWild ungulates are an important part of terrestrial ecosystems and play a critical role in maintaining ecosystem health and integrity. In many grassland ecosystems that are habituated by wild ungulates, the coexistence of domestic ungulates has created a conflict over grazing resources. Solving this conflict requires a balanced and sustainable policy that satisfies both the needs of wildlife protection and food production. Here, we assess the optimal grassland livestock carrying capacity of an alpine grassland on the Qinghai-Tibet Plateau, given the coexistence of wild populations of kiangs (Equus kiang) and Tibetan gazelles (Procapra picticaudata), two key species grazing in this region. We use kriging and the MaxEnt method to estimate the population sizes of kiangs and Tibetan gazelles in Maduo County, Qinghai Province. We then convert the estimated population size of the two species into sheep units and calculate the residual carrying capacity for livestock grazing. We show that after accounting for the grazing need for kiangs and Tibetan gazelles, grassland in Maduo is capable of supporting 420,641 sheep units, which is slightly more than the current livestock population. However, the residual carrying capacity is highly uneven across the region, and overgrazing is found in many areas of Maduo, especially in northern Maduo. This research provides a useful framework for planning sustainable livestock farming for the Qinghai-Tibet Plateau and other regions facing wildlife-livestock conflict.

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