Standardized staining methods: Feulgen-Rossenbeck reaction for desoxyribonucleic acid and periodic acid-Schiff (PAS) procedure

2002 ◽  
Vol 77 (3) ◽  
pp. 121-125 ◽  
Author(s):  
H O Lyon ◽  
E K Schulte ◽  
P Prento ◽  
M R Barer ◽  
M-C Béné
Keyword(s):  
Periodic Acid ◽  
Desoxyribonucleic Acid ◽  
Periodic Acid Schiff ◽  
Staining Methods

Standardized staining methods: Feulgen-Rossenbeck reaction for desoxyribonucleic acid and periodic acid-Schiff (PAS) procedure

2002 ◽  
Vol 77 (3) ◽  
pp. 121-125
Author(s):  
H. O. Lyon ◽  
E. K. Schulte ◽  
P. Prento ◽  
M. R. Barer ◽  
M-C Béné
Keyword(s):  
Periodic Acid ◽  
Desoxyribonucleic Acid ◽  
Periodic Acid Schiff ◽  
Staining Methods

Some Observations upon the Cytology of the Pars Distalis of the Surgically-removed Human Pituitary

1956 ◽  
Vol s3-97 (39) ◽  
pp. 379-391
Author(s):  
C. L. FOSTER
Keyword(s):  
Periodic Acid ◽  
Cell Types ◽  
Post Mortem ◽  
Β Cells ◽  
Human Pituitary ◽  
Periodic Acid Schiff ◽  
Golgi Bodies ◽  
Lipid Inclusions ◽  
Staining Methods ◽  
Human Anterior Pituitary

Human anterior pituitary tissue that had been removed at operation and immediately fixed was examined by a number of cytological and histochemical methods and by phase contrast and electron microscopy, and compared with similar material obtained post mortem. The general histological picture of good post-mortem material (not more than 4 hours post mortem) compared well with the surgically-removed tissue. For the study of silver impregnations of the Golgi substance, however, material removed at operation was found to be greatly superior. Evidence was obtained showing that the intracellular lipid inclusions seen post mortem were not artifacts resulting from cytolytic changes. There appeared to be no relationship between these lipid bodies and the Golgi material as revealed by the Aoyama method. No unequivocal dimorphism of the Golgi bodies, correlated with α- and β-cells, such as has been reported to occur in certain other mammals, was observed. Phospholipid was present in the granules of a substantial proportion of the α-cells. It was found that most of the cells which had been designated as β-cells after the application of certain routine staining methods, and most of the Gram-positive cells, reacted positively to the Periodic acid Schiff test: these cells could therefore be regarded as true β- or mucoid cells. A method for the demonstration in frozen sections of the cell-types, together with the lipid inclusions, is described.

scholarly journals MICROSCOPIC AND HISTOCHEMICAL STUDIES ON THE AUER BODIES IN LEUKEMIC CELLS

Blood ◽  
1950 ◽  
Vol 5 (9) ◽  
pp. 847-863 ◽  
Author(s):  
G. ADOLPH ACKERMAN
Keyword(s):  
Periodic Acid ◽  
Myelogenous Leukemia ◽  
Leukemic Cells ◽  
Acute Monocytic Leukemia ◽  
Desoxyribonucleic Acid ◽  
Monocytic Leukemia ◽  
Acid Condition ◽  
Periodic Acid Schiff ◽  
Temperature Changes ◽  
Acid And Alkaline Phosphatase

Abstract (1) Seventeen cases of acute leukemia with Auer bodies have been reported. Studies were carried out on 7 cases of acute monocytic leukemia and 3 cases of subacute myelogenous leukemia. (2) Histochemical studies showed the Auer bodies to be oxidase, peroxidase, and periodic acid-Schiff positive; sudanophilic, slightly metachromatic and to give positive tests for acetal lipids and ribonucleic acid. (3) The Auer bodies were negative for acid and alkaline phosphatase, lipase, glycogen, desoxyribonucleic acid and were non-birefringent. (4) A change in the chemical nature of the Auer body from an acid condition to a more neutral state was noted. This change corresponded with the changes of the normal cytoplasmic granulation of the myelocytes and monocytes during maturation. (5) The effects of cellular movements, trauma, and temperature changes upon the Auer bodies were studied. (6) Several leukemic cells, containing Auer bodies, were studied during the process of mitosis. (7) A theory as to the formation and disintegration of the Auer bodies has been presented.

scholarly journals Relationship between the morphologic alterations of vocal cords from adult autopsies and the cause of death

2004 ◽  
Vol 59 (2) ◽  
pp. 63-66 ◽  
Author(s):  
Ana Karina Marques Salge ◽  
Eumenia Costa da Cunha Castro ◽  
Mara Lúcia Fonseca Ferraz ◽  
Marlene Antônia dos Reis ◽  
Vicente de Paula Antunes Teixeira
Keyword(s):  
Vocal Cord ◽  
Cause Of Death ◽  
Respiratory Diseases ◽  
Periodic Acid ◽  
Basal Membrane ◽  
Vocal Cords ◽  
Periodic Acid Schiff ◽  
White Ethnicity ◽  
Staining Methods ◽  
Hematoxylin Eosin

PURPOSE: The purpose of this study was to identify the possible alteration in the thickness of the epithelium basal membrane of the vocal cords and correlate it with the cause of death. METHOD: Larynxes collected from adult autopsies during the period of 1993 to 2001 were utilized. We used the hematoxylin-eosin and periodic acid-Schiff staining methods for the morphological and morphometric analysis. RESULTS: Sixty-six vocal cords were analysed; increased thickness was identified in 14 cases (21.2%), with equal proportions between the genders. Increased vocal-cord thickness was more frequent in patients of the white ethnicity (12 cases, 85.7%). Respiratory alterations were found in 10 (71.4%) of the cases with increased vocal-cord thickness. Of the patients that were maintained with mechanical ventilation before death, 7 (18.4%) had thickening of the basal membrane. Among the smokers, 9 (19.63%) had basal membrane thickening. CONCLUSION: No statistically significant differences were found between the cases in which the cause of death was related to respiratory diseases as compared to non-respiratory diseases and the thickening of the basal membrane of the vocal cords. However, new studies are needed in order to verify the etiopathogenesis of this thickening.

scholarly journals THE USE OF BASIC POLYSACCHARIDES IN HISTOCHEMISTRY AND CYTOCHEMISTRY; II. THE DEMONSTRATION OF ACIDIC POLYSACCHARIDES IN TISSUE SECTIONS USING FLUORESCEIN-LABELLED DEACETYLATED CHITIN

1962 ◽  
Vol 10 (1) ◽  
pp. 14-18 ◽  
Author(s):  
SIDNEY P. KENT ◽  
E. EDWARD EVANS
Keyword(s):  
Connective Tissue ◽  
Acridine Orange ◽  
Alcian Blue ◽  
Periodic Acid ◽  
Fluorescent Staining ◽  
Periodic Acid Schiff ◽  
Tissue Sections ◽  
Acidic Polysaccharides ◽  
Staining Methods ◽  
Formalin Fixed

A method for demonstrating acidic polysaccharides in formalin fixed parafin embedded tissue sections using fluorescein labelled deacetylated chitin is described. Epithelial and connective tissue acidic polysaccharides have been studied in a number of organs. The distribution of acidic polysaccharides as seen with fluorescein-labelled deacetylated chitin is similar to results obtained with alcian blue-periodic acid Schiff and fluorescein-labelled Aspergillus polysaccharide. Previously reported fluorescent staining methods for acidic polysaccharides such as iron-hematoxylin-acridine orange and atabrine as well as fluorescein-labelled deacetylated chitin may prove to be useful adjuncts to the light microscopic methods of demonstrating mucins. Other possible uses of deacetylated chitin, a colorless macro-cation, in histochemistry and cytochemistry are noted.

scholarly journals CHARACTERISTIC OF SKIN MORPHOLOGY OF SUNDA PORCUPINE (Hystrix javanica) WITH SPECIAL REFERENCE TO THE CONNECTIVE TISSUE

2018 ◽  
Vol 12 (1) ◽  
Author(s):  
Andhika Yudha Prawira ◽  
Desrayni Hanadhita ◽  
Anisa Rahma ◽  
Supratikno Supratikno ◽  
Savitri Novelina ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Periodic Acid ◽  
Basal Membrane ◽  
Wide Distribution ◽  
Periodic Acid Schiff ◽  
Characteristic Type ◽  
Elastin Fiber ◽  
Staining Methods ◽  
Skin Morphology ◽  
Dominant Structure

This study was conducted to investigate the histological characteristic, type, and distribution of connective tissue in Sunda porcupine skin. The investigation was carried out in three adult of sunda porcupines at microscopic level using hematoxylin eosin, Masson thrichrome, Verhoeffs van Gieson, alcian blue pH 2.5 and periodic acid Schiff staining methods. Skin consists of epidermis, dermis hypodermis, and subcutaneous muscle. Quill follicles were the main and dominant structure as well as the specific characteristic on Sunda porcupine skin. The connective tissue was distributed well in basal membrane, dermis, quill follicle, and hypodermis with various intensity and density. The collagen was the main fiber found in the skin while the elastin fiber was not observed. The acid carbohydrate was found distributed well in the skin while the neutral carbohydrate was not detected in this study. In addition the fibers of connective tissue associated with the adipose tissue which found plentifully in quill follicles and hypodermis. The present results showed that the wide distribution of connective tissue might have an important role on the wound healing physiology of Sunda porcupine skin.

Cytochemical localization of glycogen phosphorylase activity in rat liver

1993 ◽  
Vol 51 ◽  
pp. 298-299
Author(s):  
John E. Michaels ◽  
Robert R. Cardell
Keyword(s):  
Rat Liver ◽  
Glycogen Phosphorylase ◽  
Periodic Acid ◽  
Glycogen Synthesis ◽  
Liver Glycogen ◽  
Periodic Acid Schiff ◽  
Cytochemical Localization ◽  
Key Enzyme ◽  
Staining Methods ◽  
Glycogen Phosphorylase Activity

Glycogen phosphorylase (GP) is a key enzyme in liver glycogen breakdown. GP activity is altered with its state of phosphorylation. In the current study, the intralobular distribution of GP activity was observed histochemically in frozen sections of rat liver during fasting and after stimulation of glycogen synthesis.Normal and adrenalectomized (ADX) rats were fasted overnight to reduce liver glycogen to minimal levels. Fasted ADX rats received 2 mg dexamethasone (DEX) 0-8 h prior to sacrifice to stimulate glycogen synthesis. Liver was removed, rapidly frozen in isopentane cooled in liquid nitrogen, then cryostat sectioned. In order to determine sites of GP activity, sections were incubated in medium that contained glucose 1-phosphate as substrate. Under the incubation conditions used, GP synthesized glycogen as the reaction product. Glycogen was identified by two staining methods: 1) iodine staining has been shown to be rather specific for newly synthesized glycogen produced during the histochemical procedure (Figs.1-6), whereas 2) periodic acid-Schiff (PAS) stained both native and nascent glycogen.

scholarly journals Canine Mast Cell Tumors: A Comparison of Staining Techniques

1994 ◽  
Vol 6 (4) ◽  
pp. 458-465 ◽  
Author(s):  
Jose Pedro Cannas Simoes ◽  
Polly Schoning
Keyword(s):  
Mast Cell ◽  
Toluidine Blue ◽  
Periodic Acid ◽  
Relative Effectiveness ◽  
Periodic Acid Schiff ◽  
Additional Information ◽  
Histochemical Methods ◽  
Mast Cell Tumors ◽  
Staining Methods ◽  
Safranin O

Twelve histochemical methods; affinity staining with avidin peroxidase, wheat germ agglutinin, and concavalin-A agglutinin; and an immunohistochemical stain with Kpl (CD68) antibody were compared for their relative effectiveness in staining canine mast cell tumors. Stains were compared in 28 mast cell tumors and 19 histiocytomas. The effectiveness of the histochemical methods and the lectins decreased as the mast cells became less differentiated. None of the staining methods were positive on histiocytomas. Periodic acid-Schiff (PAS) gave positive results in a few cases of mast cell tumors where other histochemical stains were negative. Although avidin peroxidase and Kpl antibody stained more mast cell tumors than any other method, they did not differ significantly from Luna's method, toluidine blue pH 0.5, toluidine blue pH 4.5, alcian blue pH 2.5, safranin O, Unna's method, and Giemsa. No stain was ideal for the diagnosis of canine mast cell tumors; however, this study suggests that the use of avidin peroxidase, Kpl antibody, and PAS may give additional information for individual poorly differentiated tumors without substantial increase in time or cost.

Ultrastructure of the Parotid Gland of the Nine-Banded Armadillo

1977 ◽  
Vol 35 ◽  
pp. 640-641
Author(s):  
J. R. Ruby
Keyword(s):  
Endoplasmic Reticulum ◽  
Parotid Gland ◽  
Periodic Acid ◽  
Acinar Cells ◽  
Duct System ◽  
Parotid Glands ◽  
Dasypus Novemcinctus ◽  
Anterior Portion ◽  
Periodic Acid Schiff ◽  
Thin Sections

Parotid glands were obtained from five adult (four male and one female) armadillos (Dasypus novemcinctus) which were perfusion-fixed. The glands were located in a position similar to that of most mammals. They extended interiorly to the anterior portion of the submandibular gland.In the light microscope, it was noted that the acini were relatively small and stained strongly positive with the periodic acid-Schiff (PAS) and alcian blue techniques, confirming the earlier results of Shackleford (1). Based on these qualities and other structural criteria, these cells have been classified as seromucous (2). The duct system was well developed. There were numerous intercalated ducts and intralobular striated ducts. The striated duct cells contained large amounts of PAS-positive substance.Thin sections revealed that the acinar cells were pyramidal in shape and contained a basally placed, slightly flattened nucleus (Fig. 1). The rough endoplasmic reticulum was also at the base of the cell.

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