Discriminative Staining Methods for the Nervous System: Luxol Fast Blue–Periodic Acid-Schiff– Hematoxylin Triple Stain and Subsidiary Staining Methods

1987 ◽  
Vol 62 (5) ◽  
pp. 305-315 ◽  
Author(s):  
Noboru Goto
Keyword(s):  
Nervous System ◽  
Periodic Acid ◽  
Periodic Acid Schiff ◽  
Luxol Fast Blue ◽  
Fast Blue ◽  
Staining Methods

Some Observations upon the Cytology of the Pars Distalis of the Surgically-removed Human Pituitary

1956 ◽  
Vol s3-97 (39) ◽  
pp. 379-391
Author(s):  
C. L. FOSTER
Keyword(s):  
Periodic Acid ◽  
Cell Types ◽  
Post Mortem ◽  
Β Cells ◽  
Human Pituitary ◽  
Periodic Acid Schiff ◽  
Golgi Bodies ◽  
Lipid Inclusions ◽  
Staining Methods ◽  
Human Anterior Pituitary

Human anterior pituitary tissue that had been removed at operation and immediately fixed was examined by a number of cytological and histochemical methods and by phase contrast and electron microscopy, and compared with similar material obtained post mortem. The general histological picture of good post-mortem material (not more than 4 hours post mortem) compared well with the surgically-removed tissue. For the study of silver impregnations of the Golgi substance, however, material removed at operation was found to be greatly superior. Evidence was obtained showing that the intracellular lipid inclusions seen post mortem were not artifacts resulting from cytolytic changes. There appeared to be no relationship between these lipid bodies and the Golgi material as revealed by the Aoyama method. No unequivocal dimorphism of the Golgi bodies, correlated with α- and β-cells, such as has been reported to occur in certain other mammals, was observed. Phospholipid was present in the granules of a substantial proportion of the α-cells. It was found that most of the cells which had been designated as β-cells after the application of certain routine staining methods, and most of the Gram-positive cells, reacted positively to the Periodic acid Schiff test: these cells could therefore be regarded as true β- or mucoid cells. A method for the demonstration in frozen sections of the cell-types, together with the lipid inclusions, is described.

scholarly journals Relationship between the morphologic alterations of vocal cords from adult autopsies and the cause of death

2004 ◽  
Vol 59 (2) ◽  
pp. 63-66 ◽  
Author(s):  
Ana Karina Marques Salge ◽  
Eumenia Costa da Cunha Castro ◽  
Mara Lúcia Fonseca Ferraz ◽  
Marlene Antônia dos Reis ◽  
Vicente de Paula Antunes Teixeira
Keyword(s):  
Vocal Cord ◽  
Cause Of Death ◽  
Respiratory Diseases ◽  
Periodic Acid ◽  
Basal Membrane ◽  
Vocal Cords ◽  
Periodic Acid Schiff ◽  
White Ethnicity ◽  
Staining Methods ◽  
Hematoxylin Eosin

PURPOSE: The purpose of this study was to identify the possible alteration in the thickness of the epithelium basal membrane of the vocal cords and correlate it with the cause of death. METHOD: Larynxes collected from adult autopsies during the period of 1993 to 2001 were utilized. We used the hematoxylin-eosin and periodic acid-Schiff staining methods for the morphological and morphometric analysis. RESULTS: Sixty-six vocal cords were analysed; increased thickness was identified in 14 cases (21.2%), with equal proportions between the genders. Increased vocal-cord thickness was more frequent in patients of the white ethnicity (12 cases, 85.7%). Respiratory alterations were found in 10 (71.4%) of the cases with increased vocal-cord thickness. Of the patients that were maintained with mechanical ventilation before death, 7 (18.4%) had thickening of the basal membrane. Among the smokers, 9 (19.63%) had basal membrane thickening. CONCLUSION: No statistically significant differences were found between the cases in which the cause of death was related to respiratory diseases as compared to non-respiratory diseases and the thickening of the basal membrane of the vocal cords. However, new studies are needed in order to verify the etiopathogenesis of this thickening.

scholarly journals Vascular malformations of central nervous system: A series from tertiary care hospital in South India

2016 ◽  
Vol 07 (02) ◽  
pp. 262-268
Author(s):  
Sudhir Babu Karri ◽  
Megha S. Uppin ◽  
A. Rajesh ◽  
K. Ashish ◽  
Suchanda Bhattacharjee ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Vascular Malformations ◽  
Demographic Data ◽  
Periodic Acid ◽  
Tertiary Care ◽  
Care Hospital ◽  
Periodic Acid Schiff ◽  
Radiological Features ◽  
Risk Of Hemorrhage

ABSTRACT Aims and Objectives: To describe clinicopathological features of surgically resected vascular malformations (VMs) of central nervous system (CNS). Materials and Methods: Histologically diagnosed cases of VMs of CNS during April 2010–April 2014 were included. Demographic data, clinical and radiological features were obtained. Hematoxylin and eosin slides were reviewed along with Verhoeff-Van Gieson (VVG), Masson’s trichrome, periodic acid-Schiff, and Perls’ stains. Morphologically, cavernomas and arteriovenous malformations (AVMs) were distinguished on the basis of vessel wall features on VVG and intervening glial parenchyma. Results: Fifty cases were diagnosed as VMs of CNS with an age range of 14–62 years. These included 36 cavernomas, 12 AVMs, 2 mixed capillary-cavernous angiomas. Most of the cavernoma patients (15/36) presented with seizures, whereas AVM patients (8/12) had a headache as the dominant symptom. Twenty-nine patients were reliably diagnosed on radiological features. Microscopic evidence of hemorrhage was seen in 24/36 cavernomas and 6/12 AVMs, as opposed to radiologic evidence of 10 and 4, respectively. Reactive gliosis was seen in 16 cavernomas. Conclusions: Histological features are important for classifying the VMs of CNS as there are no specific clinical and radiological features. Type of VM has a bearing on management, prognosis, and risk of hemorrhage.

scholarly journals THE USE OF BASIC POLYSACCHARIDES IN HISTOCHEMISTRY AND CYTOCHEMISTRY; II. THE DEMONSTRATION OF ACIDIC POLYSACCHARIDES IN TISSUE SECTIONS USING FLUORESCEIN-LABELLED DEACETYLATED CHITIN

1962 ◽  
Vol 10 (1) ◽  
pp. 14-18 ◽  
Author(s):  
SIDNEY P. KENT ◽  
E. EDWARD EVANS
Keyword(s):  
Connective Tissue ◽  
Acridine Orange ◽  
Alcian Blue ◽  
Periodic Acid ◽  
Fluorescent Staining ◽  
Periodic Acid Schiff ◽  
Tissue Sections ◽  
Acidic Polysaccharides ◽  
Staining Methods ◽  
Formalin Fixed

A method for demonstrating acidic polysaccharides in formalin fixed parafin embedded tissue sections using fluorescein labelled deacetylated chitin is described. Epithelial and connective tissue acidic polysaccharides have been studied in a number of organs. The distribution of acidic polysaccharides as seen with fluorescein-labelled deacetylated chitin is similar to results obtained with alcian blue-periodic acid Schiff and fluorescein-labelled Aspergillus polysaccharide. Previously reported fluorescent staining methods for acidic polysaccharides such as iron-hematoxylin-acridine orange and atabrine as well as fluorescein-labelled deacetylated chitin may prove to be useful adjuncts to the light microscopic methods of demonstrating mucins. Other possible uses of deacetylated chitin, a colorless macro-cation, in histochemistry and cytochemistry are noted.

Standardized staining methods: Feulgen-Rossenbeck reaction for desoxyribonucleic acid and periodic acid-Schiff (PAS) procedure

2002 ◽  
Vol 77 (3) ◽  
pp. 121-125
Author(s):  
H. O. Lyon ◽  
E. K. Schulte ◽  
P. Prento ◽  
M. R. Barer ◽  
M-C Béné
Keyword(s):  
Periodic Acid ◽  
Desoxyribonucleic Acid ◽  
Periodic Acid Schiff ◽  
Staining Methods

scholarly journals CHARACTERISTIC OF SKIN MORPHOLOGY OF SUNDA PORCUPINE (Hystrix javanica) WITH SPECIAL REFERENCE TO THE CONNECTIVE TISSUE

2018 ◽  
Vol 12 (1) ◽  
Author(s):  
Andhika Yudha Prawira ◽  
Desrayni Hanadhita ◽  
Anisa Rahma ◽  
Supratikno Supratikno ◽  
Savitri Novelina ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Periodic Acid ◽  
Basal Membrane ◽  
Wide Distribution ◽  
Periodic Acid Schiff ◽  
Characteristic Type ◽  
Elastin Fiber ◽  
Staining Methods ◽  
Skin Morphology ◽  
Dominant Structure

This study was conducted to investigate the histological characteristic, type, and distribution of connective tissue in Sunda porcupine skin. The investigation was carried out in three adult of sunda porcupines at microscopic level using hematoxylin eosin, Masson thrichrome, Verhoeffs van Gieson, alcian blue pH 2.5 and periodic acid Schiff staining methods. Skin consists of epidermis, dermis hypodermis, and subcutaneous muscle. Quill follicles were the main and dominant structure as well as the specific characteristic on Sunda porcupine skin. The connective tissue was distributed well in basal membrane, dermis, quill follicle, and hypodermis with various intensity and density. The collagen was the main fiber found in the skin while the elastin fiber was not observed. The acid carbohydrate was found distributed well in the skin while the neutral carbohydrate was not detected in this study. In addition the fibers of connective tissue associated with the adipose tissue which found plentifully in quill follicles and hypodermis. The present results showed that the wide distribution of connective tissue might have an important role on the wound healing physiology of Sunda porcupine skin.

Immunoelectron microscopic verification of a resident central-nervous-system macrophage in the rat that is not a pericyte, microglia, or type of mast cell

1994 ◽  
Vol 52 ◽  
pp. 38-39
Author(s):  
Ruth V. W. Dimlich
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Mast Cell ◽  
Periodic Acid ◽  
Multivesicular Bodies ◽  
Type Ii ◽  
Periodic Acid Schiff ◽  
Perivascular Cell ◽  
Luminal Side ◽  
First Time

One type of perivascular cell in the brains of all species so far examined has been given many different names. This has introduced a great deal of confusion into the literature. Using light microscopy this cell is autofluorescent, stains with periodic acid-Schiff (PAS), and cannot be identified by H&E that is used routinely in neuropathological analysis. Ultrastructurally this cell is on the luminal side of the basal lamina and has a non-lobulated nucleus, few mitochondria, a well defined Golgi area, and numerous lysosomes, phagosomes, and multivesicular bodies. Because of these features, the most common names for these cells have been “yellow fluorescing or Mato cells”, “perivascular or pericytal microglia”, “globular or phagocytosing pericytes” and “type II brain mast cell or neurolipomastocytes”.Application of a variety of histochemical techniques verified that these cells did not contain heparin or histamine, and identified for the first time that they also did not contain serotonin. Although granular and autofluorescent, the data confirmed that these cells were not mast cells. Immunohistochemically these cells also did not stain with OX42, an antibody specific for resting or activated microglia, or for actin as expected for pericytes.

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