EFFECTIVE SUPPRESSION OF HIV-1 GENE EXPRESSION BY A MAMMALIAN tRNA 3′ PROCESSING ENDORIBONUCLEASE AND EXTERNAL GUIDE SEQUENCE OLIGOZYMES

2001 ◽  
Vol 20 (4-7) ◽  
pp. 719-722 ◽  
Author(s):  
Michiko Kitano ◽  
Jaco S. Barnor ◽  
Naoko Miyano-Kurosaki ◽  
Yumihiko Endo ◽  
Masakazu Yukita ◽  
...  
Keyword(s):  
Gene Expression ◽  
Effective Suppression ◽  
Guide Sequence ◽  
Hiv 1 ◽  
External Guide Sequence

Effective suppression of HIV-1 gene expression by a mammalian tRNA 3' processing endoribonuclease and external guide sequence oligozymes

2000 ◽  
Vol 44 (1) ◽  
pp. 207-208
Author(s):  
M. Kitano ◽  
N. Miyano-Kurosaki ◽  
Y. Endo ◽  
M. Yukita ◽  
H. Takeuchi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Effective Suppression ◽  
Guide Sequence ◽  
Hiv 1 ◽  
External Guide Sequence

scholarly journals RNase P as a tool for disruption of gene expression in maize cells

2004 ◽  
Vol 380 (3) ◽  
pp. 611-616 ◽  
Author(s):  
Sunita RANGARAJAN ◽  
M. L. Stephen RAJ ◽  
J. Marcela HERNANDEZ ◽  
Erich GROTEWOLD ◽  
Venkat GOPALAN
Keyword(s):  
Gene Expression ◽  
Transient Expression ◽  
Gene Disruption ◽  
Biosynthetic Pathway ◽  
Rnase P ◽  
Flavonoid Biosynthetic Pathway ◽  
Specific Complex ◽  
Guide Sequence ◽  
Key Enzyme ◽  
External Guide Sequence

RNase P, a ribonucleoprotein responsible for the 5´ maturation of precursor tRNAs (ptRNAs) in all organisms, can be enticed to cleave any target mRNA that forms a ptRNA-like structure and sequence-specific complex when bound to an RNA, termed the EGS (external guide sequence). In the present study, F3H (flavanone 3-hydroxylase), a key enzyme in the flavonoid biosynthetic pathway that participates in the formation of red-coloured anthocyanins, was used as a target for RNase P-mediated gene disruption in maize cells. Transient expression of an EGS complementary to the F3H mRNA resulted in suppression of F3H to 29% of the control, as indicated by a reduced number of anthocyanin-accumulating cells. This decrease was not observed in experiments where a disabled mutant EGS was expressed. Our results demonstrate the potential of employing plant RNase P, in the presence of an appropriate gene-specific EGS, as a tool for targeted degradation of mRNAs.

Inhibition of no tail (ntl) gene expression in zebrafish by external guide sequence (EGS) technique

2007 ◽  
Vol 35 (2) ◽  
pp. 139-143 ◽  
Author(s):  
De-Sheng Pei ◽  
Yong-Hua Sun ◽  
Yong Long ◽  
Zuo-Yan Zhu
Keyword(s):  
Gene Expression ◽  
No Tail ◽  
Guide Sequence ◽  
External Guide Sequence

scholarly journals STING and cGAS gene expressions were downregulated among HIV-1-infected persons after antiretroviral therapy

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Lorena Leticia Peixoto de Lima ◽  
Allysson Quintino Tenório de Oliveira ◽  
Tuane Carolina Ferreira Moura ◽  
Ednelza da Silva Graça Amoras ◽  
Sandra Souza Lima ◽  
...  
Keyword(s):  
Gene Expression ◽  
Viral Load ◽  
T Cell ◽  
Antiretroviral Therapy ◽  
Cell Count ◽  
Enzyme Linked Immunosorbent Assay ◽  
Type I ◽  
Α Level ◽  
The Impact ◽  
Hiv 1

Abstract Background The HIV-1 epidemic is still considered a global public health problem, but great advances have been made in fighting it by antiretroviral therapy (ART). ART has a considerable impact on viral replication and host immunity. The production of type I interferon (IFN) is key to the innate immune response to viral infections. The STING and cGAS proteins have proven roles in the antiviral cascade. The present study aimed to evaluate the impact of ART on innate immunity, which was represented by STING and cGAS gene expression and plasma IFN-α level. Methods This cohort study evaluated a group of 33 individuals who were initially naïve to therapy and who were treated at a reference center and reassessed 12 months after starting ART. Gene expression levels and viral load were evaluated by real-time PCR, CD4+ and CD8+ T lymphocyte counts by flow cytometry, and IFN-α level by enzyme-linked immunosorbent assay. Results From before to after ART, the CD4+ T cell count and the CD4+/CD8+ ratio significantly increased (p < 0.0001), the CD8+ T cell count slightly decreased, and viral load decreased to undetectable levels in most of the group (84.85%). The expression of STING and cGAS significantly decreased (p = 0.0034 and p = 0.0001, respectively) after the use of ART, but IFN-α did not (p = 0.1558). Among the markers evaluated, the only markers that showed a correlation with each other were STING and CD4+ T at the time of the first collection. Conclusions ART provided immune recovery and viral suppression to the studied group and indirectly downregulated the STING and cGAS genes. In contrast, ART did not influence IFN-α. The expression of STING and cGAS was not correlated with the plasma level of IFN-α, which suggests that there is another pathway regulating this cytokine in addition to the STING–cGAS pathway.

scholarly journals Fourth NF-κB site in HIV-1 subtype-C LTR confers functional advantage to viral gene expression

Retrovirology ◽  
2009 ◽  
Vol 6 (S2) ◽  
Author(s):  
Mahesh Bachu ◽  
Rajesh V Murali ◽  
Anil MHKH Babu ◽  
Venkat SRK Yedavalli ◽  
Kuan-Teh Jeang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Viral Gene Expression ◽  
Viral Gene ◽  
Subtype C ◽  
Functional Advantage ◽  
Hiv 1

scholarly journals HIV-1 Tat Elongates the G1Phase and Indirectly Promotes HIV-1 Gene Expression in Cells of Glial Origin

1998 ◽  
Vol 273 (14) ◽  
pp. 8130-8136 ◽  
Author(s):  
Mondira Kundu ◽  
Sunita Sharma ◽  
Antonio De Luca ◽  
Antonio Giordano ◽  
Jay Rappaport ◽  
...  
Keyword(s):  
Gene Expression ◽  
Hiv 1 ◽  
In Cells

scholarly journals HIV-1 Infection Transcriptomics: Meta-Analysis of CD4+ T Cells Gene Expression Profiles

Viruses ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 244 ◽  
Author(s):  
Antonio Victor Campos Coelho ◽  
Rossella Gratton ◽  
João Paulo Britto de Melo ◽  
José Leandro Andrade-Santos ◽  
Rafael Lima Guimarães ◽  
...  
Keyword(s):  
Gene Expression ◽  
T Cells ◽  
Differentially Expressed Genes ◽  
Cd4 T Cells ◽  
Expression Profiles ◽  
Meta Analysis ◽  
Differentially Expressed ◽  
Rna Seq ◽  
Infected Cells ◽  
Hiv 1

HIV-1 infection elicits a complex dynamic of the expression various host genes. High throughput sequencing added an expressive amount of information regarding HIV-1 infections and pathogenesis. RNA sequencing (RNA-Seq) is currently the tool of choice to investigate gene expression in a several range of experimental setting. This study aims at performing a meta-analysis of RNA-Seq expression profiles in samples of HIV-1 infected CD4+ T cells compared to uninfected cells to assess consistently differentially expressed genes in the context of HIV-1 infection. We selected two studies (22 samples: 15 experimentally infected and 7 mock-infected). We found 208 differentially expressed genes in infected cells when compared to uninfected/mock-infected cells. This result had moderate overlap when compared to previous studies of HIV-1 infection transcriptomics, but we identified 64 genes already known to interact with HIV-1 according to the HIV-1 Human Interaction Database. A gene ontology (GO) analysis revealed enrichment of several pathways involved in immune response, cell adhesion, cell migration, inflammation, apoptosis, Wnt, Notch and ERK/MAPK signaling.

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