EB1 and EB3 regulate microtubule minus end organization and Golgi morphology

End-binding proteins (EBs) are the core components of microtubule plus end tracking protein complexes, but it is currently unknown whether they are essential for mammalian microtubule organization. Here, by using CRISPR/Cas9-mediated knockout technology, we generated stable cell lines lacking EB2 and EB3 and the C-terminal partner-binding half of EB1. These cell lines show only mild defects in cell division and microtubule polymerization. However, the length of CAMSAP2-decorated stretches at noncentrosomal microtubule minus ends in these cells is reduced, microtubules are detached from Golgi membranes, and the Golgi complex is more compact. Coorganization of microtubules and Golgi membranes depends on the EB1/EB3–myomegalin complex, which acts as membrane–microtubule tether and counteracts tight clustering of individual Golgi stacks. Disruption of EB1 and EB3 also perturbs cell migration, polarity, and the distribution of focal adhesions. EB1 and EB3 thus affect multiple interphase processes and have a major impact on microtubule minus end organization.

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Faculty Opinions recommendation of The core components of organelle biogenesis and membrane transport in the hydrogenosomes of Trichomonas vaginalis.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.724171944.793514033 ◽

2016 ◽

Author(s):

David Leitsch

Keyword(s):

Membrane Transport ◽

Trichomonas Vaginalis ◽

Organelle Biogenesis ◽

The Core ◽

Core Components

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Chapter 1: Contemporary Conflict Resolution: An Overview of the Field and the Core Components of its Educational Programs

Fundamentals of Jewish Conflict Resolution ◽

10.1515/9781618115645-004 ◽

2019 ◽

pp. 2-34

Keyword(s):

Conflict Resolution ◽

Educational Programs ◽

The Core ◽

Core Components

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The core components of education 4.0 in higher education: Three case studies in engineering education

Computers & Electrical Engineering ◽

10.1016/j.compeleceng.2021.107278 ◽

2021 ◽

Vol 93 ◽

pp. 107278

Author(s):

Jhonattan Miranda ◽

Christelle Navarrete ◽

Julieta Noguez ◽

José-Martin Molina-Espinosa ◽

María-Soledad Ramírez-Montoya ◽

...

Keyword(s):

Higher Education ◽

Engineering Education ◽

Case Studies ◽

The Core ◽

Core Components

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Paired arrangement of kinetochores together with microtubule pivoting and dynamics drive kinetochore capture in meiosis I

Scientific Reports ◽

10.1038/srep25736 ◽

2016 ◽

Vol 6 (1) ◽

Cited By ~ 10

Author(s):

Gheorghe Cojoc ◽

Ana-Maria Florescu ◽

Alexander Krull ◽

Anna H. Klemm ◽

Nenad Pavin ◽

...

Keyword(s):

Cell Division ◽

Fission Yeast ◽

General Feature ◽

Protein Complexes ◽

Spindle Pole ◽

Single Object ◽

Homologous Chromosomes ◽

Angular Movement ◽

Spindle Microtubules ◽

Meiosis I

Abstract Kinetochores are protein complexes on the chromosomes, whose function as linkers between spindle microtubules and chromosomes is crucial for proper cell division. The mechanisms that facilitate kinetochore capture by microtubules are still unclear. In the present study, we combine experiments and theory to explore the mechanisms of kinetochore capture at the onset of meiosis I in fission yeast. We show that kinetochores on homologous chromosomes move together, microtubules are dynamic and pivot around the spindle pole, and the average capture time is 3–4 minutes. Our theory describes paired kinetochores on homologous chromosomes as a single object, as well as angular movement of microtubules and their dynamics. For the experimentally measured parameters, the model reproduces the measured capture kinetics and shows that the paired configuration of kinetochores accelerates capture, whereas microtubule pivoting and dynamics have a smaller contribution. Kinetochore pairing may be a general feature that increases capture efficiency in meiotic cells.

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Semantic Desktop for the End-UserSemantic Desktop für Anwender

i-com ◽

10.1524/icom.2009.0033 ◽

2009 ◽

Vol 8 (3) ◽

pp. 25-32 ◽

Cited By ~ 2

Author(s):

Gunnar Aastrand Grimnes ◽

Benjamin Adrian ◽

Sven Schwarz ◽

Heiko Maus ◽

Kinga Schumacher ◽

...

Keyword(s):

Knowledge Management ◽

Knowledge Work ◽

Personal Knowledge ◽

Term Study ◽

The Core ◽

Personal Knowledge Management ◽

Long Term Study ◽

Core Components

AbstractThis article describes the Semantic Desktop. We give insights into the core services that aim to improve personal knowledge management on the desktop. We describe these core components of our Semantic Desktop system and give evaluation results. Results of a long-term study reveal effects of using the Semantic Desktop on personal knowledge work.

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The Design of ZigBee Wireless Network for the Detection Platform of the Cylinders

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.241-244.2504 ◽

2012 ◽

Vol 241-244 ◽

pp. 2504-2509

Author(s):

Yan Li ◽

Qiao Xiang Gu

Keyword(s):

Real Time ◽

Wireless Network ◽

Environmental Conditions ◽

Protocol Stack ◽

The Real ◽

The Core ◽

Core Components ◽

Real Time Detection ◽

Cable Connection

The equipment, called detection platform of the cylinders, is used for detecting cylinders so that cylinders can be at ease use. In order to transmit the real-time detection data to PC for further processing, the platform should be connected with PC. Cable connection, in some production and environmental conditions, is limited. Under the circumstance, building wireless network is the better choice. Through comparative studying, ZigBee is chosen to be the technology for building wireless network. ZigBee chip and ZigBee2006 protocol stack are the core components in the ZigBee nodes.

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Cytokinesis and Midzone Microtubule Organization inCaenorhabditis elegans Require the Kinesin-like Protein ZEN-4

Molecular Biology of the Cell ◽

10.1091/mbc.9.8.2037 ◽

1998 ◽

Vol 9 (8) ◽

pp. 2037-2049 ◽

Cited By ~ 193

Author(s):

William B. Raich ◽

Adrienne N. Moran ◽

Joel H. Rothman ◽

Jeff Hardin

Keyword(s):

Cell Division ◽

Null Allele ◽

Time Lapse ◽

Equatorial Region ◽

Microtubule Organization ◽

Dividing Cells ◽

Spindle Midzone ◽

Cross Links ◽

Complete Cell

Members of the MKLP1 subfamily of kinesin motor proteins localize to the equatorial region of the spindle midzone and are capable of bundling antiparallel microtubules in vitro. Despite these intriguing characteristics, it is unclear what role these kinesins play in dividing cells, particularly within the context of a developing embryo. Here, we report the identification of a null allele ofzen-4, an MKLP1 homologue in the nematodeCaenorhabditis elegans, and demonstrate that ZEN-4 is essential for cytokinesis. Embryos deprived of ZEN-4 form multinucleate single-celled embryos as they continue to cycle through mitosis but fail to complete cell division. Initiation of the cytokinetic furrow occurs at the normal time and place, but furrow propagation halts prematurely. Time-lapse recordings and microtubule staining reveal that the cytokinesis defect is preceded by the dissociation of the midzone microtubules. We show that ZEN-4 protein localizes to the spindle midzone during anaphase and persists at the midbody region throughout cytokinesis. We propose that ZEN-4 directly cross-links the midzone microtubules and suggest that these microtubules are required for the completion of cytokinesis.

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The Activity of Lipid A and Core Components of Bacterial Lipopolysaccharides in the Prevention of the Hypersensitive Response in Pepper

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi.1997.10.7.926 ◽

1997 ◽

Vol 10 (7) ◽

pp. 926-928 ◽

Cited By ~ 45

Author(s):

Mari-Anne Newman ◽

Michael J. Daniels ◽

J. Maxwell Dow

Keyword(s):

Hypersensitive Response ◽

Xanthomonas Campestris ◽

Lipid A ◽

Enteric Bacteria ◽

Core Oligosaccharide ◽

The Core ◽

Minimal Structure ◽

Core Components ◽

Pre Treatment ◽

Bacterial Lipopolysaccharides

Pre-treatment of leaves of pepper (Capsicum annuum) with lipopolysaccharide (LPS) preparations from enteric bacteria and Xanthomonas campestris could prevent the hypersensitive response caused by an avirulent X. campestris strain. By use of a range of deep-rough mutants, the minimal structure in Salmonella LPS responsible for the elicitation of this effect was determined to be lipid A attached to a disaccharide of 2-keto-3-deoxyoctulosonate; lipid A alone and the free core oligosaccharide from a Salmonella Ra mutant were not effective. For Xanthomonas, the core oligosaccharide alone had activity although lipid A was not effective. The results suggest that pepper cells can recognize different structures within bacterial LPS to trigger alterations in plant response to avirulent pathogens.

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