scholarly journals OSMIUM ZINC IODIDE REACTIVE SITES IN THE EPIDERMAL LANGERHANS CELL

1969 ◽  
Vol 43 (1) ◽  
pp. 80-89 ◽  
Author(s):  
Gustav Niebauer ◽  
Walter S. Krawczyk ◽  
Richard L. Kidd ◽  
George F. Wilgram
Keyword(s):  
Langerhans Cells ◽  
Osmium Tetroxide ◽  
Close Relation ◽  
Uranyl Acetate ◽  
Langerhans Cell ◽  
Cell Periphery ◽  
Golgi Region ◽  
Acetate Lead ◽  
Normal Epidermis ◽  
Zinc Iodide

Fixation of epidermis with a mixture of osmium tetroxide and zinc iodide (OsO4-ZnI2) for 24 hr renders the central periodic lamella of the Langerhans cell granule (LCG), the Golgi region, and the nuclear envelope of epidermal Langerhans cells preferentially visible. The use of this technique on Langerhans cells in normal epidermis and in epidermis of patients with histiocytosis (Letterer-Siwe disease) allows a broader visualization of the LCG's than was heretofore possible with routine glutaraldehyde-osmium tetroxide fixation and uranyl acetate-lead staining. The identical staining of Golgi apparatus and LCG favors the view that there is close relation between the Golgi area and the LCG's. Different staining characteristics of the LCG's near the Golgi region and at the cell periphery, respectively, may suggest that the LCG undergoes changes on its way from the Golgi area towards the extracellular space. The hypothesis is advanced that the material which is heavily impregnated with metal after fixation with OsO4-ZnI2 might be a lipid.

Ruthenium tetroxide fixation comparison of bacterial surface ultrastructure

1986 ◽  
Vol 44 ◽  
pp. 256-257 ◽  
Author(s):  
R. C. Caughey ◽  
M. A. Miller
Keyword(s):  
Pathogenic Bacteria ◽  
Osmium Tetroxide ◽  
Uranyl Acetate ◽  
Surface Structures ◽  
Ruthenium Tetroxide ◽  
Bacterial Surface ◽  
Anionic Polymers ◽  
Membrane Surfaces ◽  
Acetate Lead ◽  
Different Strains

Ruthenium tetroxide is known to improve overall contrast and preservation of membranes. It is a cationic dye which has an affinity for strongly positive anionic polymers and thus more effectively preserves exopolysaccharide materials such as glycocalyx on bacterial membrane surfaces. This study was undertaken to determine the usefulness of this technique over conventional fixation procedures in the study of bacterial surface structures that are important in pathogenicity.Different strains of pathogenic bacteria (possessing various surface structures) were routinely fixed in 2.5% glutareldehyde and 1% osmium tetroxide, dehydrated in a graded series of ethanols, and embedded in Epon 812 epoxy resin. Half of each sample was fixed in 0.05% ruthenium tetroxide (pH7.1) instead of osmium tetroxide, and then dehydrated and embedded as above. All of the samples were sectioned and post stained in uranyl acetate/lead citrate.

Immunobiology of Langerhans' Cells Migrating into Aural Cholesteatomas

1984 ◽  
Vol 92 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Jan E. Veldman ◽  
Cees E. Visser ◽  
Henk-Jan Schuurman ◽  
John C. M. J. De Groot ◽  
Egbert H. Huizing
Keyword(s):  
T Cell ◽  
T Lymphocytes ◽  
T Lymphocyte ◽  
Langerhans Cells ◽  
Close Relation ◽  
Cell Interaction ◽  
Langerhans Cell ◽  
Membrane Receptors ◽  
Cell Populations ◽  
Cell Functions

Immunohistochemical and submicroscopic analyses of human cholesteatoma matrices reveal the presence of Langerhans' cells and T lymphocytes. Through cell-to-cell Interaction, Langerhans' cells probably play a key role In skin-related disorders, including cholesteatomas. They originate from a mobile cell population of monocyte origin and migrate into and out of the body's lining. Their custodial function is always carried out In close relation with T lymphocytes. Various monoclonal antibodies directed against Langerhans' cell and T lymphocyte membrane receptors reveal the presence of these cell populations in cholesteatoma matrices but not in the tympanic membrane. Langerhans' cell and T cell traffic through cholesteatomas are discussed in relation to the pathogenesis, natural course, and recurrence rate of cholesteatomas. Through Immunopathologic evaluation the clinical aggressiveness of a cholesteatoma will probably become predictable. Medical manipulation of Langerhans' cell and T cell functions-as an adjuvant to surgery-may have consequences for the future handling of cholesteatomas.

Interrelationships between Golgi, GERL and synaptic vesicles in the nerve cells of insect and gastropod ganglia

1976 ◽  
Vol 22 (2) ◽  
pp. 435-453
Author(s):  
N.J. Lane ◽  
L.S. Swales
Keyword(s):  
Endoplasmic Reticulum ◽  
Golgi Complex ◽  
Synaptic Vesicles ◽  
Osmium Tetroxide ◽  
Positive Response ◽  
Schistocerca Gregaria ◽  
Multivesicular Bodies ◽  
Golgi Region ◽  
Limnaea Stagnalis ◽  
Zinc Iodide

In addition to demonstrating synaptic vesicles, staining with the zinc-iodide-osmium tetroxide (ZIO) method reveals the presence of positively reacting GERL membranes in association with the Golgi complex and lysosomes in the nerve cell bodies within ganglia from the locust Schistocerca gregaria and the gastropod molluscs, Limnaea stagnalis and Helix aspersa. A positive response to ZIO occurs in certain Golgi vesicles and saccules, in GERL (Golgi-endoplasmic-reticulum-lysosomes), in multivesicular bodies as well as residual bodies and in small vesicles and cisternae of axonal smooth endoplasmic reticllum (ER). The interrelationships between these organelles are considered in view of the similarity of the ZIO localization to phosphatase-rich sites in the neuronal perikarya and with respect to the possibility that components of the synaptic vesicles are formed in the Golgi region of the cell and migrate via the axonal smooth ER to the synaptic regions.

Ultrastructure of the Mongolian Gerbil Spermatozoa

1973 ◽  
Vol 31 ◽  
pp. 636-637
Author(s):  
S. K. Majumdar ◽  
T. S. Thomas
Keyword(s):  
Mongolian Gerbil ◽  
Osmium Tetroxide ◽  
Uranyl Acetate ◽  
Meriones Unguiculatus ◽  
Lead Citrate ◽  
Sodium Cacodylate ◽  
Acetate Lead ◽  
Mammalian Spermatozoa

With the recent broadening of the study of fertilization in vitro and in vivo, the importance of spermatozoon ultrastructure has been greatly emphasized. A great deal of research has been conducted with mammalian spermatozoa. However, no information is available on the Mongolian gerbil (Meriones unguiculatus). Small blocks of testis and epididymis of mature males were fixed in 4% glutaraldehyde and post-fixed in 1% osmium tetroxide adiusted to pH 7.2 with 0.1 M sodium cacodylate. After dehydration, the tissues were embedded in Epon. Sections were cut with glass knives on a Porter-Blum ultramicrotome stained with uranyl acetate-lead-citrate, and examined with an RCA EMU-3G electron microscope.The head of the Mongolian gerbil is falciform and contains a nucleus, an acrosome, and membranous envelopes (Figs. 1 & 7).

Cellular Contacts Within the Interhemal Membrane of the Rat Placenta at Term

1974 ◽  
Vol 32 ◽  
pp. 146-147
Author(s):  
William P. Jollie
Keyword(s):  
Osmium Tetroxide ◽  
Uranyl Acetate ◽  
Capillary Endothelium ◽  
En Bloc ◽  
Aldehyde Fixation ◽  
Chorioallantoic Placenta

By routine EM preparative techniques, the tissues which, collectively, separate maternal and fetal bloods in the fully formed chorioallantoic placenta of the rat have been shown to consist of three chorionic layers, or trophoblast, and a layer of allantoic capillary endothelium [Fig. 1]. Relationships between these layers are best demonstrated by special techniques, viz., cacodylate-buffered aldehyde fixation, collidine-buffered osmium tetroxide postfixation, and en bloc staining with uranyl acetate. By using this method on placentas at term, the cells of the outermost chorionic layer (Trophoblast 1) appear to be attached to each other by means of maculae adherentes which sometimes occur in clusters [Fig. 2].

Membranous alterations in the cytoplasm and nucleus in a primitive neuroectodermal tumor of the brain

1978 ◽  
Vol 36 (2) ◽  
pp. 628-629
Author(s):  
C. N. Sun ◽  
C. Araoz ◽  
H. J. White
Keyword(s):  
Nuclear Envelope ◽  
Tumor Cells ◽  
Osmium Tetroxide ◽  
Primitive Neuroectodermal Tumor ◽  
Uranyl Acetate ◽  
Neuroectodermal Tumor ◽  
Annulate Lamellae ◽  
Lead Citrate ◽  
Thin Sections ◽  
The Brain

The ultrastructure of a cerebral primitive neuroectodermal tumor has been reported previously. In the present case, we will present some unusual previously unreported membranous structures and alterations in the cytoplasm and nucleus of the tumor cells.Specimens were cut into small pieces about 1 mm3 and immediately fixed in 4% glutaraldehyde in phosphate buffer for two hours, then post-fixed in 1% buffered osmium tetroxide for one hour. After dehydration, tissues were embedded in Epon 812. Thin sections were stained with uranyl acetate and lead citrate.In the cytoplasm of the tumor cells, we found paired cisternae (Fig. 1) and annulate lamellae (Fig. 2) noting that the annulate lamellae were sometimes associated with the outer nuclear envelope (Fig. 3). These membranous structures have been reported in other tumor cells. In our case, mitochondrial to nuclear envelope fusions were often noted (Fig. 4). Although this phenomenon was reported in an oncocytoma, their frequency in the present study is quite striking.

Cytoplasmic Invaginations in Trophoblasts and Epithelial Cells of Rat

1971 ◽  
Vol 29 ◽  
pp. 524-525
Author(s):  
Iracema M. Baccarini
Keyword(s):  
Osmium Tetroxide ◽  
Uranyl Acetate ◽  
Nuclear Inclusions ◽  
Mucous Cells ◽  
Electron Microscopic ◽  
Cervical Epithelium ◽  
Intact Membrane ◽  
Microscopic Studies ◽  
Abnormal Cells ◽  
Nuclear Features

Some morphological nuclear features (invaginations) in normal and abnormal cells have been described in several electron microscopic studies. They have been referred to by others as blebs, loops, pockets, sheets, bodies, nuclear inclusions and cytoplasmic invaginations. Identical appearing structures were found in cells of the uterine cervical epithelium, in trophoblasts of blastocysts and in trophoblasts of rat placenta.Methods. Uterine cervix (normal rats), rat placenta (9-10 days gestation) and blastocyst were placed in 3% glutarahdehyde for 3 hours. The tissue was washed in phosphate buffer for 24 hours, postfixed in 1%. buffered osmium tetroxide for 1-2 hours and embedded in epon araldite. Sections were double stained with uranyl acetate and lead citrate and viewed in E. M. Siemens 200.Observations. Nuclear invaginations were found in basal, parabasal and mucous cells of the cervix epithelium, in trophoblasts of blastocyst and in trophoblasts of placenta. An oval, round or elongated invagination contained heterogenously cytoplasm surrounded by a double intact membrane; usually several invaginations were found in the same nucleus.

The Ultrastructure of Metastatic Human Mammary Carcinoma After Prolonged Estrogen Therapy

1967 ◽  
Vol 25 ◽  
pp. 180-181
Author(s):  
John H.L. Watson ◽  
John L. Swedo ◽  
R.W. Talley
Keyword(s):  
Mammary Carcinoma ◽  
Osmium Tetroxide ◽  
Estrogen Therapy ◽  
Ultrastructural Level ◽  
Uranyl Acetate ◽  
Human Mammary Carcinoma ◽  
Surgical Biopsy ◽  
Lead Hydroxide ◽  
Preliminary Study ◽  
Hormonal Therapies

A preliminary study of human mammary carcinoma on the ultrastructural level is reported for a metastatic, subcutaneous nodule, obtained as a surgical biopsy. The patient's tumor had responded favorably to a series of hormonal therapies, including androgens, estrogens, progestins, and corticoids for recurring nodules over eight years. The pertinent nodule was removed from the region of the gluteal maximus, two weeks following stilbestrol therapy. It was about 1.5 cms in diameter, and was located within the dermis. Pieces from it were fixed immediately in cold fixatives: phosphate buffered osmium tetroxide, glutaraldehyde, and paraformaldehyde. Embedment in each case was in Vestopal W. Contrasting was done with combinations of uranyl acetate and lead hydroxide.

Reactivity of Pulmonary Alveolar Cells to Crocidolite Asbestos Fibers

1982 ◽  
Vol 40 ◽  
pp. 334-335
Author(s):  
Charles L. Sanders ◽  
Roy R. Adee
Keyword(s):  
Osmium Tetroxide ◽  
Intratracheal Instillation ◽  
Uranyl Acetate ◽  
Fiber Suspension ◽  
Nacl Solution ◽  
Alveolar Cells ◽  
Asbestos Fibers ◽  
Fischer Rats ◽  
Mineral Silicates

Asbestos is a generic name for a group of hydrated mineral silicates that occur naturally in a fibrous form. The early interactions of asbestos fibers with alveolar cells in large part determines their long-term toxicity. Young adult, SPF, Fischer rats were given a single intratracheal instillation of 2 mg crocidolite asbestos suspended in 0.5 ml of 0.9% NaCl solution. About 80% of the fibers had lengths of less than 10 ym as measured on light micrographs of the fiber suspension. Two rats were killed at 3 hr, 1 d and 1, 4, 8, 12 and 16 wk after instillation and the lungs instilled with 8 ml McDowell - Trumps at 20 cm H2O. Lung tissue was dehydrated and sputtered coated with palladium-gold for SEM or post-fixed in osmium tetroxide, embedded in epoxy resin and sections stained with uranyl acetate and lead citrate for TEM.

Cell Reactivity Pattern of the Guinea Pig Cecal Mucosa Evidenced by the ZIO Technique

1982 ◽  
Vol 40 ◽  
pp. 50-51
Author(s):  
Juan Mora-Galindo ◽  
Jorge Arauz-Contreras
Keyword(s):  
Guinea Pig ◽  
Phase Contrast ◽  
Osmium Tetroxide ◽  
Cell Types ◽  
Cell Reactivity ◽  
Transmission Electron ◽  
Neural Tissues ◽  
Maturation Stages ◽  
Zinc Iodide ◽  
Cecal Mucosa

The zinc iodide-osmium tetroxide (ZIO) technique is presently employed to study both, neural and non neural tissues. Precipitates depends on cell types and possibly cell metabol ism as well.Guinea pig cecal mucosa, already known to be composed of epithelium with cells at different maturation stages and lamina propria which i s formed by morphologically and functionally heterogeneous cell population, was studied to determine the pat tern of ZIO impregnation. For this, adult Guinea pg cecal mucosa was fixed with buffered 1.2 5% g 1 utara 1 dehyde before incubation with ZIO for 16 hours, a t 4°C in the dark. Further steps involved a quick sample dehydration in graded ethanols, embedding in Epon 812 and sectioning to observe the unstained material under a phase contrast light microscope (LM) and a transmission electron microscope (TEM).

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