scholarly journals STAINING TOXOPLASMA GONDII WITH FLUORESCEIN-LABELLED ANTIBODY

1957 ◽  
Vol 105 (6) ◽  
pp. 557-573 ◽  
Author(s):  
Morris Goldman

A new serologic test for antibodies to Toxoplasma is described, which is based upon inhibition of specific staining with fluorescent antibody. In performing the test, a mixture of the test serum and known fluorescein-labelled antiserum is added to a dried smear of toxoplasms for 1 hour at 37°C. The smear is then rinsed and examined with a fluorescence microscope. Reduction in the brightness of fluorescence, as compared to that of a negative control slide, indicates the presence of antibody in the test serum. A comparison of the results of this test with those of the methylene blue dye test showed a strong parallelism between the two sets of results. On the other hand, the complement-fixation test for toxoplasmosis did not yield nearly as many positives as the inhibition test. The specificity of the new test was studied by comparing it with dye test results and clinical histories in human patients, and by testing a group of animals immunized with a variety of non-Toxoplasma antigens. No evidence of cross-reactions was obtained in the latter series. Some advantages and disadvantages of the inhibition test are discussed.

Author(s):  
M. V. Tomashev ◽  
A. S. Avdeev ◽  
M. V. Krasnova

The article presents the results of the use of adaptive testing in higher education institutions with the example of the LMS Stimul implemented in Polzunov Altai State Technical University. The advantages and disadvantages of testing as an assessment method are considered. On the base of analysis of modern educational trends, the conclusion is made about the relevance of the issue of adaptive testing, taking into account the personal mastering of the material by the student. A description of a testing system that making a permanent adaptation of the test to customize the test for the knowledge of a particular student is given. The variant of the adaptive testing algorithm is given. A comparison of the results obtained using the conventional test and the adaptive test is described. Analysis of the test results is carried out in the context of topics, categories and types of questions. Based on the given examples, a conclusion is made on the effectiveness of adaptive testing.


2021 ◽  
Vol 10 (2) ◽  
pp. 245
Author(s):  
Andrzej Sieśkiewicz ◽  
Tomasz Łysoń ◽  
Marek Rogowski ◽  
Marek Bielecki ◽  
Ewa Gindzienska-Sieskiewicz ◽  
...  

Purpose: The risk of epiphora after medial maxillectomy with lacrimal duct transection is difficult to assess. The data available in the literature are inconclusive due to various operating techniques used by the authors of medical publications, different additional procedures aimed at improving tear drainage after maxillectomy, and a variety of lacrimal duct patency assessment techniques. The aim of our work was to assess the anatomical and functional patency of lacrimal ducts after medial maxillectomy without performing additional procedures to improve tear drainage as well as comparison of the results obtained with different assessment tests. Materials and methods: 21 patients who underwent medial maxillectomy in the years 2016–2019 were assessed for discomfort and epiphora based on patients’ own reports and basic clinical examination, lacrimal duct rinse test, the Munk score, and a modified endoscopic Jones I test. Results: Gradually increasing the sensitivity of the assessment method resulted in an increase in the number of patients with potential tear drainage disorders, starting from 0% in the rinsing test, 4.8% self-reported tearing complaints, 14.3% Munk score, and 19% modified endoscopic Jones I test. Conclusions: The study results revealed that a small fraction of patients tend to report epiphora as a consequence of medial maxillectomy themselves. Subtle functional disorders, which are not particularly bothersome to patients, are more common. More sensitive lacrimal duct patency tests reveal more cases of tear drainage disorders. The results of studies assessing the incidence of epiphora after medial maxillectomy appear to depend on the type of test used.


2014 ◽  
Vol 933 ◽  
pp. 82-85
Author(s):  
Shi Feng Wang ◽  
Yi Xiang Yue ◽  
Jin Fang

In this paper, the actual operation of the superconducting motor and electrical parameters, a detailed analysis of the advantages and disadvantages of the existing quench detection method. After that I proposed an innovative detection method - voltage phase difference detection. On this basis, the design phase detection method based on the voltage difference quench detection and protection systems, based on stand-alone test and NI development platform test results, we verify its feasibility of the voltage phase difference detection method, and great superiority.


1960 ◽  
Vol 111 (6) ◽  
pp. 785-800 ◽  
Author(s):  
Aron E. Szulman

The mapping out of the histologic distribution of blood group antigens A and B in human tissues was performed by means of the fluorescent antibody technique. Human hyperimmune sera were conjugated with fluorescein isocyanate and applied to frozen sections of human material obtained at autopsy or after surgical removal. The material examined encompassed A, B, and AB subjects. In the latter the anti-A and the anti-B conjugate elicited the same picture. Group O tissues were used for controls and were uniformly negative. The secretor status of subjects was determined from the saliva or by the Lewis typing of erythrocytes. The results fall into the following main divisions: Endothelia of Vessels.—Widespread localization was demonstrated in the cell walls of endothelium of capillaries, veins, arteries, and of sinusoidal cells of spleen. Stratified Epithelia.—These showed good outlining of cells of the Malpighian (and the granular, when present) layers. In transitional epithelia, cells of the basal and contiguous layers gave specific staining. Mucus-Secreting Apparatus.—Positive staining was obtained in glands, goblet cells, and secreting surface epithelia. In non-secretors there was no identifiable antigen with the important exception of the deeper parts of gastric foveolae, deeper parts of crypts of Lieberkühn of bowel mucosa and Brunner's glands of the duodenum. Various Organs of Secretion and Excretion.—The pancreas (exocrine portion) and the sweat glands were found to produce the antigen irrespectively of secretor status. Breast, prostate, and endometrial glands on the other hand apparently secrete the antigen in conformity with the subject's secretor:non-secretor make-up. Thus the secretor:non-secretor status governs principally the antigens associated with mucous secretions and this in most but not all locations. The possible nature of this control is briefly discussed.


Author(s):  
С.А. Свердлов

Проблема понимания текстов и способов его оценки является одной из фундаментальных для современной психологии, педагогики, лингвистики. На настоящий момент имеется большое количество попыток выработки метода оценки понимания текстов, который бы отражал объективную картину интериоризации материала текста читателем, и вместе с тем подходил бы для стандартизации и применения на широком диапазоне текстов различного содержания и структуры, по возможности с минимальными затратами ресурсов оценщика и самого читателя. В настоящей статье раскрываются современные подходы к оценке понимания текстов, их преимущества и недостатки, и формулируется новый перспективный метод оценки понимания текстов, использующий в своём основании модель Латентного размещения Дирихле. Данный метод затем проходит исследование валидности через сравнение результатов его применения и наиболее часто используемых современных методов оценки понимания текстов, делается вывод о его применимости в реальных условиях и перспективах использования в спектре прикладных задач. The problem of understanding texts and methods of assessing it is one of the fundamental for modern psychology, pedagogy, linguistics. At the moment, there are a large number of attempts to develop a method for assessing the understanding of texts, which would reflect an objective picture of the internalization of the material of the text by the reader, and at the same time would be suitable for standardization and application on a wide range of texts of different content and structure, if possible with minimal expenditure of resources of the evaluator and the reader himself. This article reveals modern approaches to assessing text understanding, their advantages and disadvantages, and formulates a new promising method for assessing text understanding, based on the Dirichlet Latent Placement model. This method then goes through a validity study through a comparison of the results of its application and the most commonly used modern methods for assessing text understanding, a conclusion is made about its applicability in real conditions and the prospects for use in a range of applied problems.


DYNA ◽  
2020 ◽  
Vol 87 (212) ◽  
pp. 226-235
Author(s):  
Giovanna Monique Alelvan ◽  
Daniela Toro Rojas ◽  
Amanda Cristina Pedron Rossato ◽  
Raydel Lorenzo Reinaldo ◽  
Manoel Porfirio Cordão Neto

Problems involving large deformations are the focus of numerical modeling researches in recent decades due to the challenge of finding a kinematic appropriate description of the continuum. In recent years, different formulations have been used to describe such problems as the Arbitrary Lagrangian Eulerian (ALE) method and the Material Point Method (MPM). These two methods allow to perform dynamic analyzes involving large deformations. In this way, this work aims to present a comparison of problems applied to Geotechnics involving large deformations and large displacements, using MPM and FEM associated with the ALE method. For this purpose, three problems are simulated: sliding of blocks on an inclined plane, runout process of sand and instability of a slope using the MPM and the FEM associated with the ALE method. In all cases a comparison of the results is presented, and the advantages and disadvantages of each method are discussed.


2011 ◽  
Vol 317-319 ◽  
pp. 1119-1122
Author(s):  
Nao Sheng Qiao ◽  
Chun Mei Yao ◽  
Yan Li ◽  
Xue Juan Chen

Until now, no effective method to measure CCD photoelectric parameters. To solve the problem, a novel measurement method that combines hardware with software is proposed, and it can let hardware and software collaborate with each other to finish assignment together. Firstly, the ideal relation between output voltage and input exposure quantity of CCD is given. Secondly, some main photoelectric parameters of CCD are elaborated. Then based on the principle, test system of CCD photoelectric parameters that combines hardware with software is designed. Lastly, the real relation between output voltage and input exposure quantity of CCD is given, and the test results of experiment of each photoelectric parameter are obtained according to the relation. What’s more, analysis and comparison of the results of the experiment are given in this paper. The method is reliable and the precision is high in the experiment.


2017 ◽  
Vol 2017 ◽  
pp. 1-11 ◽  
Author(s):  
Jialin Zhou ◽  
Xin Zhang ◽  
Hongsheng Jiang ◽  
Chunhao Lyu ◽  
Erwin Oh

This paper examines shaft and base grouted concrete piles by conducting vertical static load tests (SLTs) and dynamic load tests. Three concrete piles with shaft and base grouting, with base grouting only, and without grouting techniques were selected, and compressive SLTs were conducted. Two piles with grouting were also assessed with dynamic load tests. Another two uplift SLTs were conducted to one shaft and base grouted pile and one pile without grouting. Traditional presentations were provided to check whether the bored piles reached the design requirement. Interpretations of test results were also provided to determine the ultimate pile capacity. Results from these 5 SLT programs indicated that double-tangent and DeBeer's methods are close to each other, and Chin's method overestimates the pile capacity. Comparison of the results from the SLTs and dynamic load tests shows that the results from Chin's method are close to dynamic results, and Mazurkiewicz's method overestimates for friction resistance. The results also demonstrate that base and shaft grouted pile and base grouted pile increase by 9.82% and 2.89% in compressive capacity, respectively, and compared to the uplift SLTs; there is a 15.7% increment in pile capacity after using base and shaft grouting technology.


2014 ◽  
Vol 540 ◽  
pp. 96-105 ◽  
Author(s):  
Hong Mei Sun ◽  
Li Wei Hu ◽  
Jia Wen

Wind turbines typically run in harsh environments region, but the general requirements in the case of work unattended. Therefore, the brake system is a key component to protect the safe operation of wind turbines. When the wind turbine over speed occurs, overload or other abnormal conditions, the brake system needs to start immediately, so that the whole unit into the shutdown state to ensure crew safety. This article describes the role of wind turbine brake system, composition, operation principle, comparing the advantages and disadvantages of the most commonly used active and passive spindle brake doubly-fed wind turbine. Completion of a wind turbine import passive brake design, manufacturing localization, combined with wind turbine machine operating parameters, developed analytical test program brakes and the test results, and for the test questions are designed to improve.


2014 ◽  
Vol 519-520 ◽  
pp. 1605-1608
Author(s):  
Tatyana N. Gnitetskaya ◽  
Elena B. Ivanova ◽  
Cherednychenko Alexander

There is a big gap between level of multimedia possibilities and level of using multimedia in training physics at the universities. In this paper the advantages and disadvantages of Power Point physics lectures are described. It is noted that one from important advantages is coordination of Power Point with Learning Management System. The examples of writing formulas, drawing graphics in time are presented in this paper. It is described the animated image illustrating the operation of a heat engine with the help of aniline drop. Test results allow us to give in this paper some guidelines on slide design to make information perceiving from the screen easier for students.


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