scholarly journals A BLOOD COAGULATION ABNORMALITY IN RABBITS DEFICIENT IN THE SIXTH COMPONENT OF COMPLEMENT (C6) AND ITS CORRECTION BY PURIFIED C6

1971 ◽  
Vol 134 (6) ◽  
pp. 1591-1600 ◽  
Author(s):  
Theodore S. Zimmerman ◽  
Carlos M. Arroyave ◽  
Hans J. Müller-Eberhard
Keyword(s):  
Blood Coagulation ◽  
Time Course ◽  
Bleeding Time ◽  
Clotting Factor ◽  
Clotting Time ◽  
Platelet Factor ◽  
Coagulation Abnormality ◽  
Normal Human ◽  
Time Specific ◽  
Blood Coagulation Abnormality

Evidence for the involvement of the sixth component of complement (C6) in normal blood coagulation is provided by the description of a coagulation abnormality in rabbits with a genetic C6 deficiency and by its correction with highly purified preparations of C6. Whole blood clotting time in glass or plastic was prolonged and prothrombin consumption was decreased in blood from the deficient animals. Other parameters of blood coagulation were normal, including prothrombin time, partial thromboplastin time, specific clotting factor activities, platelet factor III function, platelet count, and bleeding time. Clotting time and prothrombin consumption became normal when physiologic amounts of highly purified C6 were added to the deficient blood. Partial consumption of C6 hemolytic activity, with a time course similar to the consumption of prothrombin, was demonstrated during the clotting of normal human blood.

Effects of intravenous administration of a fat emulsion on blood coagulation in dogs

1959 ◽  
Vol 196 (5) ◽  
pp. 1015-1019 ◽  
Author(s):  
Harrison H. Shoulders ◽  
Robert C. Hartmann ◽  
H. C. Meng
Keyword(s):  
Blood Coagulation ◽  
Intravenous Administration ◽  
Intravenous Infusion ◽  
Bleeding Time ◽  
Plasma Fibrinogen ◽  
Fibrinogen Concentration ◽  
Clotting Time ◽  
Clot Retraction ◽  
Fat Emulsion ◽  
Abnormal Bleeding

A fat emulsion prepared for intravenous administration has been studied with regard to its effect upon blood coagulation in dogs. The most characteristic effects found during intravenous infusion of this material at a rate of 1 ml/min. were thrombocytopenia and marked shortening of clotting time. These effects were invariably observed in animals which had not previously received the emulsion. When subsequent infusions were given within 3 hours, no significant changes were observed. When the interval was extended to 1–13 days, variable responses occurred, at times characterized by pronounced hypocoagulability. If the repeat infusion was given 2 weeks or more after the initial one, the effects were similar to those observed during the first infusion. The prothrombin and thrombin clotting times and plasma fibrinogen concentration were not greatly altered during the infusion. Abnormal bleeding time, ‘prothrombin utilization’ and clot retraction accompanied the thrombocytopenia.

scholarly journals The Timing of Expression of Blood Coagulation Abnormality in Patients Treated with Warfarin and S-1 Concomitantly

2010 ◽  
Vol 130 (7) ◽  
pp. 955-960 ◽  
Author(s):  
Takaaki YAMADA ◽  
Hiroyuki WATANABE ◽  
Takahisa YANO ◽  
Toshiharu NONAKA ◽  
Atsushi TAKADA ◽  
...  
Keyword(s):  
Blood Coagulation ◽  
Coagulation Abnormality ◽  
Blood Coagulation Abnormality

scholarly journals Role of Serotonin in Blood Coagulation

1957 ◽  
Vol 189 (3) ◽  
pp. 470-474 ◽  
Author(s):  
W. L. Milne ◽  
S. H. Cohn
Keyword(s):  
Blood Coagulation ◽  
Therapeutic Effect ◽  
Whole Blood ◽  
Active Role ◽  
Clotting Time ◽  
Platelet Factor ◽  
Coagulation Defect ◽  
Increased Sensitivity

Effects of serotonin (5HTA) were studied a) in whole blood ( in vitro) from both normal and x-irradiated animal, and b) in isolated clotting systems of plasma and purified fibrinogen. 5HTA was found to play an active role in blood coagulation in addition to its previously demonstrated role as a vasoconstrictor. In whole blood from normal and irradiated animals, and in platelet-deficient plasma, 5HTA acts in a manner similar to platelet factor 2 in accelerating the conversion of fibrinogen to fibrin. In a purified fibrinogen solution, 5HTA inhibits the fibrinogen-fibrin reaction, and thus differs from platelet factor 2. This may be due to the absence of an antithrombin in the purified fibrinogen solution. Serotonin appears to have a therapeutic effect on the postirradiation coagulation defect in that it decreases the prolonged heparin clotting time. This increased sensitivity to heparin during the postirradiation thrombocytopenia may be due to a deficiency of serotonin, which acts as an antagonist to heparin (an antithrombin). The action of 5HTA is postulated as an antagonist of an antithrombin which blocks the fibrinogen to fibrin reaction.

Blood Coagulation Inhibitor in a Snake Plasma (Bothrops jararaca)

1973 ◽  
Vol 30 (01) ◽  
pp. 106-113 ◽  
Author(s):  
Linda Nahas ◽  
Ferruccio Betti ◽  
Aura S. Kamiguti ◽  
Hissae Sato
Keyword(s):  
Human Plasma ◽  
Blood Coagulation ◽  
Antithrombin Iii ◽  
The Other ◽  
Normal Human Plasma ◽  
Clotting Time ◽  
Bovine Thrombin ◽  
Heat Stable ◽  
Coagulation Inhibitor ◽  
Normal Human

SummaryExperiments with plasmas samples from the snake B. jararaca and X. merremii have shown that the two differ markedly with respect to their reaction to bovine thrombin. The plasma of X. merremii appears to react in a manner very similar to that of normal human plasma both using the thrombin clotting test and using the antithrombin III assay of Astrup and Darling (Biggs and Macfariane 1962). The plasma of B. jararaca on the other hand, contains, in addition to antithrombin III, an inhibitor which resembles mammalian heparin. This inhibitor prolongs the thrombin clotting time of human and X. merremii plasmas, is recorded by the antithrombin assay and is neutralized by protamine. The inhibitor is however, not identical with mammalian heparin since it is not adsorbed by BaSO4 and Al(OH)3 (which adsorb heparin) and it is heat labil whereas mammalian heparin is heat stable.

The clotting plot—an analysis of the kinetics of the blood clotting reactions

1969 ◽  
Vol 173 (1032) ◽  
pp. 421-441 ◽  
Keyword(s):  
Blood Coagulation ◽  
Blood Clotting ◽  
Clotting Factor ◽  
Clotting Time ◽  
Clotting Factors ◽  
Chain Reaction ◽  
Time Test ◽  
Proportional Variation ◽  
Kinetics Of ◽  
Mathematical Relations

Despite the complexity of blood coagulation reactions there are two linear mathematical relations between clotting time and concentration of clotting factors. One of these relations connects clotting time and reciprocal of concentration, and holds only when several clotting factors are reduced simultaneously. Experiments were carried out to determine which combinations of reduced factors were associated with reciprocal linearity. The hypothesis is put forward that the chain reaction which results in clotting is viewed as a whole in the clotting time test and that linearity results when the whole reaction runs in a balanced and organized manner. Proportional variation of a single clotting factor will seldom have this effect.

Essential Athrombia

1975 ◽  
Vol 33 (02) ◽  
pp. 278-285 ◽  
Author(s):  
Şeref Inceman ◽  
Yücel Tangün
Keyword(s):  
Platelet Aggregation ◽  
Bleeding Time ◽  
Bleeding Tendency ◽  
Clot Retraction ◽  
Platelet Factor ◽  
Prolonged Bleeding Time ◽  
Normal Platelet ◽  
Aggregation Response ◽  
Platelet Disorder ◽  
Fibrinogen Content

SummaryA constitutional platelet function disorder in a twelve-year-old girl characterized by a lifelong bleeding tendency, prolonged bleeding time, normal platelet count, normal clot retraction, normal platelet factor 3 activity and impaired platelet aggregation was reported.Platelet aggregation, studied turbidimetrically, was absent in the presence of usual doses of ADP (1–4 μM), although a small wave of primary aggregation was obtained by very large ADP concentrations (25–50 μM). The platelets were also unresponsive to epinephrine, thrombin and diluted collagen suspensions. But an almost normal aggregation response occurred with strong collagen suspensions. The platelets responded to Ristocetin. Pelease of platelet ADP was found to be normal by collagen and thrombin, but impaired by kaolin. Platelet fibrinogen content was normal.The present case, investigated with recent methods, confirms the existence of a type of primary functional platelet disorder characterized solely by an aggregation defect, described in 1955 and 1962 under the name of “essential athrombia.”

Effect of Temperature, Velocity Gradient and I.V. Heparin on in Vitro Blood Coagulation and Platelet Aggregation

1967 ◽  
Vol 17 (01/02) ◽  
pp. 112-119 ◽  
Author(s):  
L Dintenfass ◽  
M. C Rozenberg
Keyword(s):  
Blood Coagulation ◽  
Velocity Gradient ◽  
Elevated Temperatures ◽  
Morphological Changes ◽  
Effect Of Temperature ◽  
Clotting Time ◽  
Progressive Change ◽  
Aggregation Of Platelets ◽  
Microscopic Techniques

SummaryA study of blood coagulation was carried out by observing changes in the blood viscosity of blood coagulating in the cone-in-cone viscometer. The clots were investigated by microscopic techniques.Immediately after blood is obtained by venepuncture, viscosity of blood remains constant for a certain “latent” period. The duration of this period depends not only on the intrinsic properties of the blood sample, but also on temperature and rate of shear used during blood storage. An increase of temperature decreases the clotting time ; also, an increase in the rate of shear decreases the clotting time.It is confirmed that morphological changes take place in blood coagula as a function of the velocity gradient at which such coagulation takes place. There is a progressive change from the red clot to white thrombus as the rates of shear increase. Aggregation of platelets increases as the rate of shear increases.This pattern is maintained with changes of temperature, although aggregation of platelets appears to be increased at elevated temperatures.Intravenously added heparin affects the clotting time and the aggregation of platelets in in vitro coagulation.

Observations on a Relationship between Steroid Metabolism and the Concentration of Plasma Fibrinogen

1963 ◽  
Vol 10 (02) ◽  
pp. 400-405 ◽  
Author(s):  
B. A Amundson ◽  
L. O Pilgeram
Keyword(s):  
Blood Coagulation ◽  
Methyl Ether ◽  
Broad Spectrum ◽  
Human Subjects ◽  
Plasma Fibrinogen ◽  
Steroid Metabolism ◽  
Specific Site ◽  
Chain Reaction ◽  
Normal Human ◽  
A Chain

SummaryEnovid (5 mg norethynodrel and 0.075 mg ethynylestradiol-3-methyl ether) therapy in young normal human subjects causes an increase in plasma fibrinogen of 32.4% (P >C 0.001). Consideration of this effect together with other effects of Enovid on the activity of specific blood coagulatory factors suggests that the steroids are exerting their effect at a specific site of the blood coagulation and/or fibrinolytic system. The broad spectrum of changes which are induced by the steroids may be attributed to a combination of a chain reaction and feed-back control.

The Anticoagulant and Hemorrhagic Effects of DHG, a New Depolymerized Holothurian Glycosaminoglycan, on Experimental Hemodialysis in Dogs

1997 ◽  
Vol 77 (06) ◽  
pp. 1148-1153 ◽  
Author(s):  
Kazuhisa G Minamiguchi ◽  
Keiko T Kitazato ◽  
Eiji Sasaki ◽  
Hideki Nagase ◽  
Kenji Kitazato
Keyword(s):  
Low Molecular Weight Heparin ◽  
Bleeding Time ◽  
Activated Partial Thromboplastin Time ◽  
Low Molecular Weight ◽  
Nafamostat Mesilate ◽  
Extracorporeal Circuit ◽  
Clotting Time ◽  
Prolonged Bleeding Time ◽  
Hemorrhagic Risk ◽  
Effective Doses

SummaryWe studied the use of depolymerized holothurian glycosaminoglycan (DHG) as an anticoagulant in experimental beagle-dog hemodialysis using a hollow-fiber dialyzer compared to that using unfractionated heparin (UFH), low-molecular-weight heparin (LMWH), and nafamostat mesilate (FUT). Effectiveness was based on 5 h hemodialysis and no marked clot deposition in the extracorporeal circuit. At effective doses, UFH and LMWH significantly prolonged template bleeding time, in sharp contrast to FUT and DHG, which scarcely prolonged bleeding time during hemodialysis. DHG prolonged activated partial thromboplastin time (APTT) about 6 times that of normal plasma and prolonged thrombin clotting time (TCT) markedly; FUT showed marked APTT prolongation but hardly prolonged TCT in the hemodialysis circuit at the effective dose. The anticoagulant profile of DHG thus differs completely from that of FUT. These results suggest that DHG may be useful as anticoagulant for hemodialysis with low hemorrhagic risk.

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