scholarly journals A polymorphic microsatellite in the tumor necrosis factor alpha promoter identifies an allele unique to the NZW mouse strain.

1990 ◽  
Vol 171 (6) ◽  
pp. 2141-2146 ◽  
Author(s):  
C V Jongeneel ◽  
H Acha-Orbea ◽  
T Blankenstein
Keyword(s):  
Tumor Necrosis ◽  
Genetic Defect ◽  
Inbred Strains ◽  
Mouse Strains ◽  
Chain Reaction ◽  
Necrosis Factor Alpha ◽  
Factor Alpha ◽  
Polymerase Chain ◽  
Polymorphic Microsatellite ◽  
Necrosis Factor

We have amplified a (CA)n:(GT)n microsatellite from the TNF promoters of a panel of mouse strains using the polymerase chain reaction. The length of the microsatellites was polymorphic, with eight alleles observed among 15 inbred strains bearing seven distinct H-2 haplotypes, and four outbred strains. In B10 congenic strains, the TNF allele detected by microsatellite polymorphism segregated with the MHC, and in recombinant haplotypes (NOD, NZW), it segregated with H-2D. The TNF allele found in the NZW strain (H-2z) was distinct from those of all other haplotypes, consistent with the hypothesis that this strain may carry a genetic defect in TNF production.

scholarly journals DETEKSI GEN IL-6 DAN TNF-α DENGAN METODE PCR PADA PENDERITA HEPATITIS B DI LABORATORIUM KLINIK MAXIMA KOTA KENDARI

2021 ◽  
Vol 7 (1) ◽  
pp. 21-28
Author(s):  
Sanatang Abbas ◽  
Sri Anggarini Rasyid ◽  
Tiara Mayang Pratiwi Lio
Keyword(s):  
Tumor Necrosis Factor ◽  
Hepatitis B ◽  
Interleukin 6 ◽  
Tumor Necrosis ◽  
Virus Hepatitis ◽  
Necrosis Factor Alpha ◽  
Tnf Α ◽  
Factor Alpha ◽  
Polymerase Chain ◽  
Necrosis Factor

Penyakit Hepatitis B adalah inflamasi yang terjadi pada organ hati yang dapat disebabkan oleh virus hepatitis B. Pada saat terjadi inflamasi sitokin yang ada dalam tubuh akan merespon atau mengenali jenis patogen berupa virus yang masuk ke dalam tubuh. Tumor Necrosis Factor (TNF-α) adalah salah satu sitokin pro-inflamasi yang berperan dalam proses inflamasi hati, dan Interleukin-6 (IL-6) adalah sitokin yang disekresikan dari jaringan tubuh pada fase infeksi akut atau kronik. Tujuan dari penelitian ini adalah untuk mendeteksi gen TNF-α dan IL-6 pada penderita hepatitis B dengan metode polymerase chain reaction (PCR). Jenis penelitian yang digunakan dalam penelitian ini adalah semi kuantitatif, dengan desain penelitian eksperimental. Populasi pada penelitian adalah seluruh penderita suspek yang melakukan pemeriksaan rapid Hepatitis B (HbsAg) di Laboratorium Klinik Maxima Kota Kendari sebanyak 7 orang. Teknik penarikan sampel menggunakan total sampling dengan kriteria inklusi sampel yaitu pasien yang tidak memiliki riwayat penyakit lain selain hepatitis B. Berdasarkan hasil penelitian diketahui bahwa dari ketujuh sampel penderita hepatitis B yang diperiksa menggunakan metode PCR 3 sampel dengan hasil positif (45%) terhadap gen TNF-α dan 7 (100%) hasil negative terhadap gen Interleukin 6 (IL-6). Sehingga dapat di simpulkan bahwa jenis sitokin yang berperan saat terjadi inflamasi ketika seseorang terinfeksi Virus Hepatitis B adalah Tumor Necrosis Factor Alpha (TNF-α).

scholarly journals Polymorphisms in Tumour Necrosis Factor Alpha (TNFα) Gene in Patients with Acute Pancreatitis

2010 ◽  
Vol 2010 ◽  
pp. 1-6 ◽  
Author(s):  
Gül Özhan ◽  
Hakan T. Yanar ◽  
Cemalettin Ertekin ◽  
Buket Alpertunga
Keyword(s):  
Acute Pancreatitis ◽  
Tumour Necrosis Factor ◽  
Tumour Necrosis ◽  
Fragment Length Polymorphism ◽  
Chain Reaction ◽  
Necrosis Factor Alpha ◽  
Severe Pancreatitis ◽  
Factor Alpha ◽  
Polymerase Chain ◽  
Necrosis Factor

Proinflammatory cytokines, such as tumour necrosis factorα(TNFα), play fundamental roles in the pathogenesis of acute pancreatitis (AP). The aim of this study was to determine if polymorphisms in the TNFαgene are associated with AP. Two polymorphisms located in the promoter region (positions−308and−238) in TNFαgene were determined using polymerase chain reaction- (PCR-) restriction fragment length polymorphism (RFLP) methods in 103 patients with AP and 92 healthy controls. Odds ratios (ORs) and 95% confidence intervals (CI) were estimated using logistic regression analysis adjusted for age, sex, BMI and smoking. The frequencies of TNFαpolymorphisms were both similar in patients with mild or severe pancreatitis, so were in pancreatitis patients and in controls. We suggest that both SNPs of TNFαare not genetic risk factor for AP susceptibility (OR=1.63; 95% CI: 1.13−4.01 for TNFα−308and OR=0.86; 95% CI: 0.75−1.77 for TNFα−238).

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