scholarly journals Spontaneous atopic dermatitis in mice expressing an inducible thymic stromal lymphopoietin transgene specifically in the skin

2005 ◽  
Vol 202 (4) ◽  
pp. 541-549 ◽  
Author(s):  
Jane Yoo ◽  
Miyuki Omori ◽  
Dora Gyarmati ◽  
Baohua Zhou ◽  
Theingi Aye ◽  
...  
Keyword(s):  
Animal Model ◽  
Atopic Dermatitis ◽  
Transgenic Mice ◽  
Elevated Serum ◽  
Allergic Inflammation ◽  
Disease Process ◽  
Allergic Diseases ◽  
Serum Levels ◽  
Thymic Stromal Lymphopoietin ◽  
Common Disease

The cytokine thymic stromal lymphopoietin (TSLP) has recently been implicated in the pathogenesis of atopic dermatitis (AD) and other allergic diseases in humans. To further characterize its role in this disease process, transgenic mice were generated that express a keratinocyte-specific, tetracycline-inducible TSLP transgene. Skin-specific overexpression of TSLP resulted in an AD-like phenotype, with the development of eczematous lesions containing inflammatory dermal cellular infiltrates, a dramatic increase in Th2 CD4+ T cells expressing cutaneous homing receptors, and elevated serum levels of IgE. These transgenic mice demonstrate that TSLP can initiate a cascade of allergic inflammation in the skin and provide a valuable animal model for future study of this common disease.

scholarly journals Elevated Serum Levels of Thymic Stromal Lymphopoietin in Wheezing Children Infected with Human Metapneumovirus

2017 ◽  
Vol 70 (2) ◽  
pp. 161-166 ◽  
Author(s):  
Wenjing Gu ◽  
Yuqing Wang ◽  
Chuangli Hao ◽  
Xinxing Zhang ◽  
Yongdong Yan ◽  
...  
Keyword(s):  
Elevated Serum ◽  
Serum Levels ◽  
Human Metapneumovirus ◽  
Thymic Stromal Lymphopoietin

scholarly journals Hyperactivity and Learning Deficits in Transgenic Mice Bearing a Human Mutant Thyroid Hormone β1 Receptor Gene

1998 ◽  
Vol 5 (4) ◽  
pp. 289-301 ◽  
Author(s):  
Michael P. McDonald ◽  
Rosemary Wong ◽  
Gregory Goldstein ◽  
Bruce Weintraub ◽  
Sheue-yann Cheng ◽  
...  
Keyword(s):  
Thyroid Hormone ◽  
Transgenic Mice ◽  
Sustained Attention ◽  
Receptor Gene ◽  
Operant Chamber ◽  
Elevated Serum ◽  
Vigilance Task ◽  
Serum Levels ◽  
Wild Type ◽  
Wild Type Littermate

Resistance to thyroid hormone (RTH) is a human syndrome mapped to the thyroid receptor β(TRβ) gene on chromosome 3, representing a mutation of the ligandbinding domain of the TRβ gene. The syndrome is characterized by reduced tissue responsiveness to thyroid hormone and elevated serum levels of thyroid hormones. A common behavioral phenotype associated with RTH is attention deficit hyperactivity disorder (ADHD). To test the hypothesis that RTH produces attention deficits and/or hyperactivity, transgenic mice expressing a mutant TRβ gene were generated. The present experiment tested RTH transgenic mice from the PV kindred on behavioral tasks relevant to the primary features of ADHD: hyperactivity, sustained attention (vigilance), learning, and impulsivity. Male transgenic mice showed elevated locomotor activity in an open field compared to male wild-type littermate controls. Both male and female transgenic mice exhibited impaired learning of an autoshaping task, compared to wild-type controls. On a vigilance task in an operant chamber, there were no differences between transgenics and controls on the proportion of hits, response latency, or duration of stimulus tolerated. On an operant go/no-go task measuring sustained attention and impulsivity, there were no differences between controls and transgenics. These results indicate that transgenic mice bearing a mutant human TRβ gene demonstrate several behavioral characteristics of ADHD and may serve a valuable heuristic role in elucidating possible candidate genes in converging pathways for other causes of ADHD.

scholarly journals Skin tissue expression and serum level of thymic stromal lymphopoietin in patients with psoriasis vulgaris

2019 ◽  
Vol 11 (1) ◽  
Author(s):  
Oki Suwarsa ◽  
Hartati Purbo Dharmadji ◽  
Endang Sutedja ◽  
Lengga Herlina ◽  
Putri Reno Sori ◽  
...  
Keyword(s):  
Psoriasis Vulgaris ◽  
Allergic Diseases ◽  
Serum Levels ◽  
Tissue Expression ◽  
Thymic Stromal Lymphopoietin ◽  
Enzyme Linked Immunosorbent Assay ◽  
Lesional Skin ◽  
Healthy Control ◽  
Polymerase Chain ◽  
The Difference

Thymic stromal lymphopoietin (TSLP) is known to be associated with allergic diseases. It is also suggested that TSLP has a role in autoimmune diseases such as psoriasis; however, the associated pathways remain unknown. There is currently little information on TSLP in psoriasis vulgaris. We investigated TSLP expressions on lesional and non-lesional skin of psoriasis vulgaris patients using reverse transcription- polymerase chain reaction. TSLP level was also investigated in serum from psoriasis vulgaris patients compared to healthy control using enzyme-linked immunosorbent assay. TSLP expression was higher in lesional skin (1.90) compared to non-lesional skin (1.76); however, the difference was not statistically significant (P>0.05). TSLP serum levels were significantly higher in psoriasis patients (287.40 pg/dL) as compared to controls (114.70 pg/dL) (P<0.05). This study concluded that TSLP levels in the serum of psoriasis vulgaris patients are higher than controls. TSLP was also found in keratinocyte of psoriasis patients, the expression was higher in the lesional compared to non-lesional skin; however, this difference is statistically insignificant. These findings suggest that TSLP may play a role in the pathogenesis of psoriasis vulgaris, but its exact role remains unclear.

scholarly journals Neuroinflammatory Gene Expression Pattern Is Similar between Allergic Rhinitis and Atopic Dermatitis but Distinct from Atopic Asthma

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Paulina Sobkowiak ◽  
Wojciech Langwiński ◽  
Joanna Nowakowska ◽  
Irena Wojsyk-Banaszak ◽  
Dawid Szczepankiewicz ◽  
...  
Keyword(s):  
Gene Expression ◽  
Allergic Rhinitis ◽  
Atopic Dermatitis ◽  
Expression Pattern ◽  
Human Study ◽  
Allergic Inflammation ◽  
Allergic Diseases ◽  
Atopic Asthma ◽  
Control Group ◽  
Altered Expression

Background and Aim. Neurogenic inflammation underlies the pathogenesis of allergic diseases. Interactions between neurons with the immune cells and structural cells (airway epithelium, nasal mucosa, skin keratinocytes) undergo altered regulation during chronic inflammation. Therefore, we hypothesized that the expression of neuroinflammatory genes may be altered allergic diseases, including atopic dermatitis and allergic rhinitis, and that, compared to atopic asthma, the expression pattern may be disease-specific in pediatric patients. Methods. In the study, we included 86 children diagnosed with atopic asthma (n=25), allergic rhinitis (n=20), and atopic dermatitis (n=20) and healthy control subjects (n=21) of Caucasian origin from the Polish population. The blood leukocyte expression of 31 genes involved in neuroinflammatory response (neurotrophins, their receptors, neuropeptides, and histamine signaling pathway) was analysed using TaqMan low-density arrays. The relative expression of selected proteins from plasma was done using TaqMan Protein Assays. Statistical analysis was done using Statistica. Results. Blood expression of 31 genes related to neuroimmune interactions showed significant increase in both allergic diseases, allergic rhinitis and atopic dermatitis, in comparison to the control group. We found 12 genes significantly increased in allergic rhinitis and 9 genes in which the expression was elevated in atopic dermatitis. Moreover, 9 genes with changed expression in atopic dermatitis overlapped with those in allergic rhinitis. Atopic asthma showed 5 genes with altered expression. The peripheral expression of neuroinflammatory genes in the human study was verified in target tissues (nasal epithelium and skin) in a rat model of allergic inflammation. Conclusions. A common pattern of neuroinflammatory gene expression between allergic rhinitis and atopic dermatitis may reflect similar changes in sensory nerve function during chronic allergic inflammation.

scholarly journals Association between Type 2 Diabetes, Obesity and Key Immunological Components of IgE-mediated Inflammation

Folia Medica ◽  
2017 ◽  
Vol 59 (2) ◽  
pp. 159-164 ◽  
Author(s):  
Raditsa N. Sokolova ◽  
Rumyana K. Yankova ◽  
Tsvetana I. Abadjieva ◽  
Tatyana A. Popova ◽  
Maria V. Ivanovska ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Glycemic Control ◽  
Allergic Inflammation ◽  
Allergic Diseases ◽  
Serum Levels ◽  
Total Serum ◽  
Control Group ◽  
Serum Ige ◽  
Ige Mediated

AbstractBackground:Changes in lifestyle and obesity in recent decades have brought about a dramatic increase in type 2 diabetes mellitus (DM2) and allergic diseases. Clinical and epidemiological studies associate obesity with epidemics of allergic diseases. The link between obesity and DM2 with immunological components of IgE-mediated allergic inflammation is not yet conclusively established.Aim:To examine the key immunological components of IgE-mediated allergic inflammation in patients with DM2 and their relationship with glycemic control and anthropometric indicators.Materials and methods:Fifty-five patients with DM2 and 32 healthy controls with normal weight and body mass index (BMI) of 18-24.9 kg/m2were included in the study. Th2-cytokine profile (serum levels of IL-4 and IL-5, pg/ml) and total serum IgE IU/ml were assessed in all participants in the study using ELISA. In patients with DM2, levels of glycated hemoglobin (HbA1c%) in the blood were also measured.Results:Serum levels of IL-4 and IL-5 are significantly higher in patients with DM2 compared to the control group. Serum levels of IL-4 and IL-5 positively correlated with BMI as well as serum levels of IL-4 with waist circumference. Total serum IgE positively correlated with HbA1c.Conclusion:Obesity and poor glycemic control in patients with DM2 affect key immunological components of IgE-mediated allergic inflammation and possibly alter the immune response to allergens and antigens.

Elevated serum levels of APRIL, but not BAFF, in patients with atopic dermatitis

2008 ◽  
Vol 17 (3) ◽  
pp. 197-202 ◽  
Author(s):  
Takashi Matsushita ◽  
Manabu Fujimoto ◽  
Takeshi Echigo ◽  
Yukiyo Matsushita ◽  
Yuka Shimada ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Elevated Serum ◽  
Serum Levels

Dysregulated microRNA expression in IL-4 transgenic mice, an animal model of atopic dermatitis

2021 ◽  
Author(s):  
Lei Bao ◽  
Cecilia S. Chau ◽  
Zhengdeng Lei ◽  
Hong Hu ◽  
Angelina G. Chan ◽  
...  
Keyword(s):  
Animal Model ◽  
Atopic Dermatitis ◽  
Transgenic Mice ◽  
Microrna Expression

scholarly journals MONTELUKAST: NEWER MODALITY IN VERNAL KERATOCONJUNCTIVITIS (VKC)

2019 ◽  
Vol 3 (6) ◽  
Author(s):  
Dr. Prachi Shukla ◽  
Dr Rajiv Gupta
Keyword(s):  
Atopic Dermatitis ◽  
Foreign Body ◽  
Positive Family History ◽  
Allergic Inflammation ◽  
Allergic Diseases ◽  
Vernal Keratoconjunctivitis ◽  
Palpebral Conjunctiva ◽  
Body Sensation ◽  
History Of ◽  
Or History

Vernal Keratoconjunctivitis (VKC) is a chronic, bilateral allergic inflammation of the conjunctiva. The disease is seasonal, recurrent & commonly seen among young boys. Common symptoms of the disease are redness, itching, lacrimation, photophobia, foreign body sensation & a characteristic ropy discharge. Signs found in VKC are conjunctival congestion, giant papillae involving upper palpebral conjunctiva & the limbus, Tranta’s dots & superficial keratitis. (1,2).  Patients with VKC frequently have a positive family history or history of allergic diseases like asthma, rhinitis, atopic dermatitis etc.(3).

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