scholarly journals The use of Trichoderma harzianum and Aspergillus niger in Indigofera zollingeriana fermentation

2019 ◽  
Vol 1402 ◽  
pp. 055017
Author(s):  
M Puspitasari ◽  
I Hadist ◽  
T Rohayati ◽  
M Royani
Keyword(s):  
Aspergillus Niger ◽  
Trichoderma Harzianum ◽  
Indigofera Zollingeriana

scholarly journals The influence of biocarrier of Aspergillus niger and Trichoderma harzianum toward vegetative growth of sorghum in the field experiment

2020 ◽  
Vol 2 (2) ◽  
pp. 28-34
Author(s):  
Arwan Sugiharto ◽  
Toga Pengihotan Napitupulu ◽  
Made Sudiana
Keyword(s):  
Aspergillus Niger ◽  
Drought Tolerance ◽  
Field Experiment ◽  
Trichoderma Harzianum ◽  
Vegetative Growth ◽  
Soil Microorganisms ◽  
Fungal Strain ◽  
Cereal Crops ◽  
Marginal Land ◽  
Stalk Diameter

Compared to other cereal crops, sorghum has a higher drought tolerance trait. However, efforts are needed to increase the productivity of sorghum, particularly in drought marginal land. One strategy to be implemented is the utilization of soil microorganisms formulated with biocarrier. Therefore, the aim of this study was to evaluate the influence of the fungal strain Aspergillus niger and Trichoderma harzianum formulated with compost and zeolite as biocarrier towards vegetative growth of sorghum. The field experiment was designed as a randomized block designed, factorial pattern with 4 replications. The first factor was selecting biocarrier, namely zeolite, compost, and a mixture of zeolite: compost (1:1). The second factor was the fungal inoculants, A. niger, and T. harzianum. The observed parameter was the growth profile of sorghum during vegetative growth, including stalk diameter and height. The results showed that the type of biocarrier, as well as the fungal strains did influence the growth of sorghum. The highest stalk diameter and height of sorghum were obtained after application of A. niger formulated with a mixture of zeolite: compost (1:1), with 17% and 41.2% higher than control, respectively. This condition shows that a mixture of zeolite and compost is seemingly able to create better micro-ecological conditions for fungal microbes to function effectively. Therefore, our findings suggested the addition of zeolite to compost for the application of biocarrier in the field experiment.

Role of Seed Mycoflora on Seed Germination of Oroxylum indicum (L.) Vent. in Kumaun region of Indian Central Himalaya

2013 ◽  
Vol 36 (1) ◽  
pp. 83-89
Author(s):  
Bhawana Pande ◽  
R. Gupta
Keyword(s):  
Aspergillus Niger ◽  
Seed Germination ◽  
Trichoderma Harzianum ◽  
Fusarium Solani ◽  
Alternaria Alternata ◽  
Alternaria Solani ◽  
Central Himalaya ◽  
Oroxylum Indicum ◽  
Indian Central

A number of fungus was isolated from the seeds of Oroxylum indicum (L.) Vent. The mycoflora associated with the seeds of this tree have not been previously reported from Kumaun region of Indian Central Himalaya. During the course of study mycoflora such as Fusarium solani, Aspergillus niger, Aspergillus nidulens, Penicillium sp., Trichoderma harzianum, Alternaria solani, Alternaria alternata, Curvolaria lunata., Stachybotryis chartarum, Acremonium sp., Rhizoctonia solani, Chaetomium globosum, Cladosporium cladoporoides and Torula allii were isolated from the seeds.

scholarly journals Fraksies en geïsoleerde verbinding uit Tecoma stans (Bignoniaceae), ’n indringerplant, het belowende aktiwiteit teen fungus fitopatogene

2017 ◽  
Vol 36 (1) ◽  
Author(s):  
Moraba M. Meelah ◽  
Ladislaus K. Mdee ◽  
Jacobus N. Eloff
Keyword(s):  
Aspergillus Niger ◽  
Rhizoctonia Solani ◽  
Trichoderma Harzianum ◽  
Aspergillus Parasiticus ◽  
Pythium Ultimum

Fungi wat plante aanval, lei tot groot verliese in plantproduksie en ook tot verliese in opbrengs nadat die produkte geoes is. Die beheer van hierdie fungi deur chemiese fungisiede lewer komplikasies vanweë menslike en omgewingstoksisiteit. Die koste en die ontwikkeling van weerstand deur plantpatogeniese fungi teen fungisiede lewer ook probleme. Sekondêre plantmetaboliete het ‘n goeie potensiaal as antifungusverbindings. Die doel van die studie was om die aktiwiteit van Tecoma stans-ekstrakte en - fraksies te bepaal en om die aktiewe verbinding te isoleer deur die bioaktiwiteit van fraksies gedurende die fraksionering te bepaal. Die dichlorometaanfraksie het die hoogste aktiwiteit gehad en die geïsoleerde verbinding se struktuur is bepaal as oleanolsuur. Die antifungusaktiwiteit is teen tien belangrike plantfunguspatogene bepaal (Aspergillus niger, Aspergillus parasiticus, Collectotrichum gloeosporoides, Fusarium oxysporium, Penicillum expansum, Penicillum janthinellum, Pythium ultimum, Phytophthora nicotiana, Trichoderma harzianum en Rhizoctonia solani). Die gemiddelde minimum inhiberende konsentrasie was 130 μg/mL. Die DCM-ekstrak en oleanolsuur was minder toksies as die positiewe kontrole berberien teen Vero-selle met LC50 waardes van 0.413 mg/mL, 0.129 mg/mL en 15.48 µg/mL onderskeidelik. Die selektiwiteitindeks van 20 met verskeie fungi dui op moontlike relatiewe veiligheid om onder gekontroleerde toestande selfs vir eetbare produkte te gebruik. Die groot massa plantmateriaal wat van hierdie indringerplant beskikbaar is, mag tot ‘n kommersieel bruikbare produk lei in die bekamping van fitopatogeniese fungi.Navorsing korrelasie: Hierdie artikel is die vertaalde weergawe en is beskikbaar gestel om ‘n breër lesersgroep te bereik. Die oorspronklike Engelse artikel is beskikbaar hier: https://doi. org/10.4102/satnt.v36i1.1489

scholarly journals Isolation and Identification of Soil Mycoflora in the Upland and Lowland Soils of Usmanu Danfodiyo University, Sokoto, Sokoto State

2018 ◽  
pp. 1-7
Author(s):  
I. Y. Tafinta ◽  
K. Sheh ◽  
H. M. Maishanu ◽  
S. S. Noma ◽  
S. A. Yusif ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Fusarium Oxysporum ◽  
Trichoderma Harzianum ◽  
Fungal Species ◽  
Potato Dextrose Agar ◽  
Morphological Features ◽  
Rhizopus Stolonifer ◽  
Isolation And Identification ◽  
Dilution Plate ◽  
Cotton Blue

A total of 14 different fungal species belonging to 7 genera were isolated from the upland and lowland soils of Usmanu Danfodiyo University, Sokoto, between October to December, 2015. The mycoflora were isolated using dilution plate technique on Potato dextrose agar amended by 1% streptomycin. Identification was made microscopically using the lacto-phenol cotton blue method and macroscopically by comparing the cultural and morphological features with the help of authentic fungal manual and taxonomic key. The identified species are; Aspergillus niger, A. flavus, A. fumigatus, A. nidulans, A. oryzae, Alternaria longifes, Fusarium oxysporum, F. solani, F. mangifera, Rhizopus stolonifer, R. orizae, Saccharomyces cerevisae, Trichoderma harzianum and Trichophytum quallinum. The highest number of fungi (50 isolates) were obtained from lowland uncultivated soils (D), followed by the upland uncultivated soils (B) with about 34 strains, and the least (22 isolates) were obtained from upland cultivated soils (A) out of the total 132 isolates. Variations between sites were statistically analyzed.

PROTEOLYTIC FUNGI FROM VIRGIN FOREST

2016 ◽  
Vol 78 (6-7) ◽  
Author(s):  
Zaidah Zainal Ariffin ◽  
Mohd Sidek Ahmad ◽  
Rosalia Pepi ◽  
Zainon Mohd Noor
Keyword(s):  
Aspergillus Niger ◽  
Proteolytic Activity ◽  
Aspergillus Flavus ◽  
Trichoderma Harzianum ◽  
Skim Milk ◽  
Morphological Characteristics ◽  
Protease Production ◽  
Penicillium Simplicissimum ◽  
Clear Zone ◽  
Virgin Forest

Microbial enzymes have continued to assist diverse reactions as biocatalysts. Soil derived microbes offer a prospective resource for such enzymes. Screening and isolation of proteolytic fungi were carried out from soil sample of a Malaysian virgin forest. Four isolates showed clear zone of protein hydrolysis on skim milk agar representing proteolytic activity. Aspergillus flavus UOA/HCPF 5774 exhibited the highest proteolytic activity with a clear zone diameter of 21 mm followed by Aspergillus niger and Trichoderma harzianum both with a clear zone of 16 mm, and Penicillium simplicissimum strain LP42 with a13 mm clear zone. Crude protease activity of 0.230 – 0.277 Units / ml for each fungus was seen after 24 hours incubation. A decline of protease production was observed after 48 hours incubation except for Aspergillus flavus UOA/HCPF 5774 which showed a drop only after 72 hours incubation. The protease producing fungi were partially identified based on their morphological characteristics, macroscopic and microscopic identification. The identification was confirmed by 18S rRNA Sequence Analysis. The four fungi protease producers were Aspergillus niger, Penicillium simplicissimum strain LP42, Aspergillus flavus UOA/HCPF 5774 and Trichoderma harzianum.

scholarly journals Antagonistic potential of rhizosphere mycoflora against Fusarial wilt of brinjal

2015 ◽  
Vol 24 (2) ◽  
pp. 137-145
Author(s):  
Romana Akter ◽  
KS Hossain ◽  
MA Bashar
Keyword(s):  
Aspergillus Niger ◽  
Fusarium Oxysporum ◽  
Aspergillus Flavus ◽  
Trichoderma Harzianum ◽  
Radial Growth ◽  
Soil Fungi ◽  
Volatile Metabolites ◽  
Penicillium Sp ◽  
Maximum Inhibition ◽  
Antagonistic Potential

Antagonistic potentials of seven rhizoshere soil fungi viz., Aspergillus flavus Link., A. fumigatus Fresen., A. niger Tiegh., A. terreus Thom., Penicillium sp., Trichoderma harzianum Refat. and T. viride Pers. were tested against the Fusarium oxysporum Schlecht. and F. solani (Mart.) Sacc. – the two pathogens, isolated from wilted roots of brinjal plants. Out of seven soil fungi Trichoderma harzianum was found most effective to control the growth of both the test pathogens. More than 50% inhibition of the test pathogens were observed with T. viride, Aspergillus niger and A. fumigatus in colony interaction. The maximum inhibition of radial growth of the test pathogens was observed with volatile metabolites and cell free culture filtrates of Trichoderma harzianum. This fungus may be exploited commercially to control the disease. Dhaka Univ. J. Biol. Sci. 24(2): 137-145, 2015 (July)

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