scholarly journals Antagonistic potential of rhizosphere mycoflora against Fusarial wilt of brinjal

2015 ◽  
Vol 24 (2) ◽  
pp. 137-145
Author(s):  
Romana Akter ◽  
KS Hossain ◽  
MA Bashar

Antagonistic potentials of seven rhizoshere soil fungi viz., Aspergillus flavus Link., A. fumigatus Fresen., A. niger Tiegh., A. terreus Thom., Penicillium sp., Trichoderma harzianum Refat. and T. viride Pers. were tested against the Fusarium oxysporum Schlecht. and F. solani (Mart.) Sacc. – the two pathogens, isolated from wilted roots of brinjal plants. Out of seven soil fungi Trichoderma harzianum was found most effective to control the growth of both the test pathogens. More than 50% inhibition of the test pathogens were observed with T. viride, Aspergillus niger and A. fumigatus in colony interaction. The maximum inhibition of radial growth of the test pathogens was observed with volatile metabolites and cell free culture filtrates of Trichoderma harzianum. This fungus may be exploited commercially to control the disease. Dhaka Univ. J. Biol. Sci. 24(2): 137-145, 2015 (July)

2019 ◽  
Vol 43 (1) ◽  
pp. 17-23 ◽  
Author(s):  
Md Delwar Hosen ◽  
Shamim Shamsi

Four soil fungi were isolated from the soil by serial dilution and were identified as Aspergillus flavus Link, A. fumigatus Fresenius, A. niger van Tieghem and Trichoderma viride Pers. The soil fungi were selected to evaluate their antagonistic potential against seed borne fungus Fusarium merismoides isolated from sesame. In dual culture colony interaction Trichoderma viride showed the highest (45.88%) growth inhibiting effect on F. merismoides followed by A. niger (40.00%), A. flavus (36.37) and A. fumigatus (30.77%). Volatile metabolites from T. viride showed the highest growth inhibiting effect on F. merismoides (67.69%) and non-volatile metabolites from T. viride showed the highest growth inhibiting effect on F. merismoides (75.00%). Journal of Bangladesh Academy of Sciences, Vol. 43, No. 1, 17-23, 2019


2019 ◽  
Vol 28 (1) ◽  
pp. 1-7
Author(s):  
Rezuana Binte Helal ◽  
Shamim Shamsi

Four antagonistic fungi were isolated from the papaya field soil by serial dilution and were identified as Aspergillus flavus Link., A. fumigatus Fresenius, A. niger van Tiegh. and Trichoderma viride Pers. The soil fungi were selected to evaluate their antagonistic potentiality against the three postharvest pathogenic fungi of Carica papaya L. following "dual culture colony interaction” and volatile and non-volatile metabolites. In dual culture colony interaction, out of four soil fungi, T. viride showed the highest growth inhibition on C. gloeosporioides (84.28%), F. nivale (95.23%) and Fusarium sp. (87.15%). On the other hand A. niger showed the highest growth inhibition on C. gloeosporioides (77.39%), F. nivale (98.63%) and Fusarium sp. (35.05%). The maximum inhibition of radial growth of C. gloeosporioides (77.64%), F. nivale (58.76%) and Fusarium sp. (79.37%). were observed in case of T. viride owing to the volatile metabolites. Whereas the maximum inhibition of radial growth of C. gloeosporioides (58.23%), F. nivale (37.43%) and Fusarium sp. (82.31%) were observed in case of A. niger owing to the volatile metabolites. The maximum inhibition of radial growth of C. gloeosporioides (90.90%), F. nivale (89.13%) and Fusarium sp. (76.84%) were observed in case of T. viride owing to the effect of non-volatile metabolites. The maximum inhibition of radial growth of C. gloeosporioides (92.42%), F. nivale (73.01%) and Fusarium sp. (68.67%) were observed in case of A. niger owing to the effect of non-volatile metabolites. Trichoderma viride and A. niger may be exploited commercially as a biocontrol agent against anthracnose and fruit rot pathogens of papaya. Dhaka Univ. J. Biol. Sci. 28(1): 1-7, 2019 (January)


2020 ◽  
Vol 29 (2) ◽  
pp. 147-153
Author(s):  
Mahfuza Aktar ◽  
Shamim Shamsi

Three antagonistic fungi were isolated from the field soil of blight infected Tagetes spp. by serial dilution method. The fungi were identified as Aspergillus flavus Link, A. niger van Tiegh. and Trichoderma viride Pers. Antagonistic potentiality of aforesaid fungi were evaluated against the pathogenic fungi of Tageteserecta L. and T. patula L. following "dual culture colony interaction" and volatile and nonvolatile metabolites. The pathogenic fungi were A. alternata, Aspergillus fumigatus and Curvularia lunata. In dual culture colony interaction, out of three soil fungi, T. viride showed the highest growth inhibition on A. alternata (71.03%), A. fumigatus (38.49%) and C. lunata (60.71%). The maximum inhibition of radial growth of A. alternata (74.55%) was observed with the culture filtrates of T. viride owing to volatile metabolites. The maximum inhibition of radial growth of A. fumigatus (37.43%) was observed with the culture filtrates of A. flavus owing to volatile metabolites. The complete inhibition of radial growth of C. lunata was observed with the culture filtrates of A. niger owing to volatile metabolites. The complete inhibition of radial growth of A. alternata was observed with nonvolatile metabolites of A. nigera and T. viride at all concentrations. The complete inhibition of radial growth of A. fumigatus was also observed with nonvolatile metabolites of A. niger at all concentrations. Aspergillus niger and T. viride may be exploited commercially as a biocontrol agent against blight pathogens of T. erecta and T. patula. Dhaka Univ. J. Biol. Sci. 29(2): 147-153, 2020 (July)


2015 ◽  
Vol 43 (2) ◽  
pp. 213-217 ◽  
Author(s):  
MT Aktar ◽  
KS Hossain ◽  
MA Bashar

Antagonistic potentials of seven rhizoshere soil fungi viz., Aspergillus flavus Link., A. fumigatus Fresen., A. niger Tiegh., A. terreus Thom., Penicillium sp., Trichoderma harzianum Refat. and T. viride Pers. were tested in opposition to six pathogenic fungi viz., Colletotrichum sp., Curvularia lunata, Fusarium moniliforme, F. oxysporum, F. semitectum and Phomopsis sp. isolated from different leaf spots and fruit rots of brinjal. Out of seven soil fungi, Trichoderma harzianum was found most effective to control the growth of all the test fungi in the study of colony interactions and effects of volatile and non-volatile metabolites. This fungus may be exploited commercially to biocontrol the diseases. DOI: http://dx.doi.org/10.3329/bjb.v43i2.21675 Bangladesh J. Bot. 43(2): 213-217, 2014 (September)


2012 ◽  
Vol 10 (1) ◽  
pp. 46-50 ◽  
Author(s):  
F. Yeasmin ◽  
M. Ashrafuzzaman ◽  
I. Hossain

Seeds of rice were treated with Garlic (Allium sativum) clove extract @ 1:0, 1:1, 1:2 dilutions in water, allamanda (Allamanda cathartica) leaf extract @ 1:1, 1:2, 1:3 dilutions in water and Provax-200 @ 0.3% for controlling seed borne fungi, where the seed samples of three rice varieties viz. Katharee, Gutee Aus and Kalijira were collected from farmer’s storages of Bangladesh. The seed germination under control ranged from 64 to 77%, where treatments resulted up to 100% germination. The identified seed borne fungi of rice were Bipolaris oryzae, Curvularia oryzae, Fusarium oxysporum, F. moniliforme, Nigrospora oryzae, Aspergillus flavus, Aspergillus niger and Penicillium sp., where prevalence of Bipolaris oryzae (7.5%) and Fusarium moniliforme (8.3%) were the maximum. All the treatments significantly reduced the seed borne fungi up to 100% over the control, where Provax was found best and was statically similar to garlic (1:1) extract against seed borne pathogen of rice.DOI: http://dx.doi.org/10.3329/agric.v10i1.11064The Agriculturists 2012; 10(1): 46-50


2021 ◽  
Vol 46 (2) ◽  
pp. 143-153
Author(s):  
Amina Khatun ◽  
Shamim Shamsi ◽  
Abul Bashar

The antagonistic potentials of four soil filamentous fungi viz., Aspergillus flavus Link, A. fumigatus Fresenius, A. niger van Tieghem and Trichoderma viride Pers. against six pathogens isolated from 14 varieties of cotton (Gossypium hirsutum L.) were evaluated by "dual culture colony interaction”, volatile and non-volatile metabolites. In dual culture colony interaction, out of four soil fungi, T. viride was found be the most effective to control the growth of the cotton pathogens. T. viride showed the highest growth inhibition against Curvularia lunata, Fusarium moniliforme, Mucor sp. and Rhizoctonia solani. On the other hand A. niger showed the highest growth inhibition against Fusarium nivale and A. fumigatus showed the maximum growth inhibition against C. gloeosporioides. The highest inhibition of radial growth of C. lunata, F. nivale and F. moniliforme was observed might be due to the secretion of volatile metabolites of T. viride whereas, the maximum inhibition of radial growth of C. gloeosporioides was observed because of the volatile metabolites of A. flavus. Mucor sp. and R. solani were inhibited due to the volatile metabolites of A. fumigatus. The greatest radial growth inhibition of C. lunata and F. moniliforme were noticed in case of T. viride owing to the effect of non-volatile metabolites. On the other hand, the radial growth of C. gloeosporioides and F. nivale were inhibited highest amount for the effect of non-volatile metabolites of A. niger, whereas highest growth inhibition of Mucor sp. and R. solani was observed due to the non-volatile effect of A. fumigatus. The present investigation suggests that the isolates of Aspergillus and Trichoderma may be further exploited as potential biocontrol agents against the fungal pathogens of cotton in field trial. Asiat. Soc. Bangladesh, Sci. 46(2): 143-153, December 2020


Author(s):  
B. Mohana ◽  
Shiva Kameshwari ◽  
M. K. Prasana Kumar

Aqueous extract of Urginea indica kunth. (Udupi acccession) was screened for antifungal activity against Fusarium oxysporum, Sclerotium rolfsii, Magnaporthe orzae, Aspergillus flavus, Alternaria alternata, Aspergillus niger and Fusarium moniliformae by poisoned food technique. The results confirmed Urginea indica extracts showed very significant antifungal activity against Fusarium oxysporum and showed significant inhibition for Sclerotium rolfsii and Magnapothea orzaea it showed no activity against Aspergillus niger and Aspergillus flavus. All the activity was evaluated to determine the lowest concentration required to inhibit visible mycelial growth of the pathogen at minmum concentration. Fusarium oxysporum showed very significant inhibition in 10% concentration (Reconfirmed) while Sclerotium showed significant inhibition in 25% concentration followed by Magnoporthe oryzae. The number of sclerotia spores formed was also reduced drastically. These results show that a potential and safe antifungal agent can be obtained from Urginea.


2015 ◽  
Vol 43 (2) ◽  
pp. 494-500 ◽  
Author(s):  
Hacer Handan ALTINOK ◽  
Oktay ERDOGAN

Fusarium oxysporum is a well-known soil-borne fungi and it is difficult to control their pathogenic strains by conventional strategies. The cultures of two strains of Trichoderma harzianum (T16 and T23) were examined in laboratory conditions and with pot experiments for the control of pathogenic strains of Fusarium oxysporum f. sp. melongenae (Fomg), Fusarium oxysporum f. sp. lycopersici (Fol), Fusarium oxysporum f. sp. niveum (Fon) and F. oxysporum f. sp. melonis (Fom). The T16 and T23 strains showed significant inhibition of mycelial growth in the pathogenic strains of F. oxysporum and the maximum inhibition were recorded when the T. harzianum strain T16 was used (72.69%). Both T. harzianum strains produced volatile and non-volatile metabolites that inhibited growth of F. oxysporum strains on PDA medium. In vitro colonization study demonstrated the root-colonizing ability of these antagonists. The interaction between T. harzianum isolates (T16 and T23) and pathogenic F. oxysporum hyphae showed no overgrowth, hyphal coiling, cell wall degradation or any hyphal penetration around any of the tested F. oxysporum hyphae. Pre-treatment of soil with T16 significantly reduced the severity of Fusarium wilt disease. The disease severity in control plants reached to 90-95% whereas those of the T16-Fomg and T16-Fol treated seedlings of eggplants were 37.74% and 47.12%, respectively, on the 21st day. In this study, while both T. harzianum isolates had a considerable antagonistic effect on the tested pathogens, T16 was found to be more successful than T23. The strong repressive effect of T. harzianum (T16) towards pathogenic Fusarium oxsporum can be applied in biological control of these pathogens.


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