Anthocyanin and Iron Absorption of Black Rice Bran Aqueous Extract using In Vitro Everted Gut Sac Method

Black rice bran extract (BRBE), containing various biologically active compounds, such as anthocyanin, has antioxidant activity and numerous pharmacological effects. Here, we aimed to confirm the immunostimulatory effects of BRBE in cyclophosphamide (CP)-induced immunosuppressed cells. Our results confirmed that BRBE exerted an immunostimulatory effect. In vitro, BRBE treatment enhanced cell proliferation, activity of natural killer cells and cytotoxic T lymphocytes, and production of CP-repressed cytokines, such as tumor necrosis factor-α, interferon-γ, interleukin (IL)-2, and IL-12, and immunoglobulins G and A in isolated splenocytes. Additionally, in vivo, BRBE treatment increased the number of immune cells, such as white blood cells, lymphocyte counts, mid-range absolute counts, and neutrophils in CP-induced immunosuppressed rats. Furthermore, BRBE increased the serum levels of abovementioned inflammatory cytokines and immunoglobulins in CP-induced immunosuppressed rats. In addition, BRBE protected against CP-mediated spleen and thymic tissue damage. Our findings suggest that BRBE could be potentially used as a component of functional food for immunity enhancement.

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The Sinergistic Effect of Black Rice Bran Extract and Glibenclamide on Protecting Renal, Hepatic, and Pancreatic Cells in Alloxan Induced Rats

International Journal of Pharmaceutical Research ◽

10.31838/ijpr/2020.12.01.113 ◽

2020 ◽

Vol 12 (01) ◽

Keyword(s):

Rice Bran ◽

Black Rice ◽

Rice Bran Extract ◽

Pancreatic Cells ◽

Black Rice Bran

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BIOLOGICAL EVALUATION OF MICROWAVE DEFATTED BLACK RICE BRAN (MDBRB) IN CCL4 INTOXICATED RATS

Journal of Food and Dairy Sciences ◽

10.21608/jfds.2015.48855 ◽

2015 ◽

Vol 6 (6) ◽

pp. 419-433

Author(s):

M. El - Bana ◽

M. El - Sayed ◽

Sahar AbdEl- Hady

Keyword(s):

Rice Bran ◽

Biological Evaluation ◽

Black Rice ◽

Black Rice Bran

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Formulation of snack bars made from black rice bran (Oryza sativa L.) and sweet potato flour (Ipomoea batatas L.)

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/828/1/012028 ◽

2021 ◽

Vol 828 (1) ◽

pp. 012028

Author(s):

Siswanti ◽

R B K Anandito ◽

E Nurhartadi ◽

R B Agustiani

Keyword(s):

Oryza Sativa ◽

Sweet Potato ◽

Rice Bran ◽

Ipomoea Batatas ◽

Oryza Sativa L ◽

Potato Flour ◽

Black Rice ◽

Sweet Potato Flour ◽

Black Rice Bran

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Maturation, iron deficiency, and ligands in enteric radioiron transport in vitro

American Journal of Physiology-Legacy Content ◽

10.1152/ajplegacy.1963.204.1.171 ◽

1963 ◽

Vol 204 (1) ◽

pp. 171-175 ◽

Cited By ~ 14

Author(s):

W. S. Ruliffson ◽

J. M. Hopping

Keyword(s):

Ascorbic Acid ◽

Iron Deficiency ◽

Iron Absorption ◽

Deficiency Anemia ◽

Anaerobic Incubation ◽

Reverse Effect ◽

Factors Affecting ◽

Everted Gut Sac

The effects in rats, of age, iron-deficiency anemia, and ascorbic acid, citrate, fluoride, and ethylenediaminetetraacetate (EDTA) on enteric radioiron transport were studied in vitro by an everted gut-sac technique. Sacs from young animals transported more than those from older ones. Proximal jejunal sacs from anemic animals transported more than similar sacs from nonanemic rats, but the reverse effect appeared in sacs formed from proximal duodenum. When added to media containing ascorbic acid or citrate, fluoride depressed transport as did anaerobic incubation in the presence of ascorbic acid. Anaerobic incubation in the presence of EDTA appeared to permit elevated transport. Ascorbic acid, citrate, and EDTA all enhanced the level of Fe59 appearing in serosal media. These results appear to agree with previously established in vivo phenomena and tend to validate the in vitro method as one of promise for further studies of factors affecting iron absorption and of the mechanism of iron absorption.

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