Isolation and screening test of indigenous endophytic bacteria from areca nut rhizosphere as plant growth promoting bacteria

Onions (Allium cepa L.) is one of the leading horticultural commodities in Indonesia and is often used as seasoning and traditional medicine. Onion has a high economic value and fluctuating prices so that domestic onion production needs to be improved, one of them with a presence of endophytic bacteria that act as plant growth promoting agent or Plant Growth Promoting Bacteria (PGPB). Endophytic bacteria isolated from the root, leaves, and bulbs. In this research has been tested endophytic bacteria of onion plants from Garut regency which has PGPB factors such as able to dissolve phosphate, and produce HCN. The results obtained 251 isolates of endophytic bacteria. Based on the characterization results, the superior isolates capable of dissolving phosphate with an average diameter of 0.45 cm is isolate II.B.1D.3, and 11 isolates capable of producing high HCN. These isolates can be used as PGPB agents so that they can be useful in increasing plant growth and onion production and biocontrol in suppressing pathogens. Keywords: PGPB, endophyte, onion, phosphate, HCN

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Potensi Bakteri Endofit dari Tanaman Sagu (Metroxylon spp.) sebagai Agens Pemacu Pertumbuhan Tanaman Padi

JURNAL BUDIDAYA PERTANIAN ◽

10.30598/jbdp.2018.14.2.75 ◽

2018 ◽

Vol 14 (2) ◽

pp. 75-80

Author(s):

Yatni Yatni ◽

Gratiana N C Tuhumury ◽

Christoffol Leiwakabessy

Keyword(s):

Plant Growth ◽

Endophytic Bacteria ◽

Growth Promoter ◽

Plant Growth Promoting Bacteria ◽

Test Results ◽

Bacterial Isolates ◽

The People ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Stems And Leaves

Sago is a staple plant of the people of Maluku and Papua, which have many benefits and advantages to continue to be developed. Endophytic bacteria are bacteria that live in plant tissues and colonize the intercellular and vascular systems. This study aims to obtain endophytic bacterial isolates from parts of the roots, stems, and leaves of sago plants which have the potential as agents for plant growth promoting bacteria. Endophytic bacteria are isolated from the roots, stems, and leaves. Based on the results of isolation, 21 isolates of endophytic bacteria were found. Then, the selection is done by hypersensitivity test and gram reaction test. The results of the selection obtained 20 isolates of endophytic bacteria that were not pathogenic. After that, it was followed by plant growth promoting test for endophytic bacterial isolates. The test results were obtained three isolates potentially as plant growth promoter that is STA1, STA6, and STA11. Keywords: endophytic bacteria, plant growth promoting, sago ABSTRAK Tanaman sagu merupakan tanaman pokok masyarakat Maluku dan Papua, yang memiliki banyak manfaat dan keunggulan untuk terus dikembangkan. Bakteri endofit adalah bakteri yang hidup di dalam jaringan tanaman dan berkoloni pada daerah ruang interseluler dan sistem vascular. Penelitian ini bertujuan untuk mendapatkan isolat bakteri endofit dari bagian akar,batang dan daun tanaman sagu yang berpotensi sebagai agens pemacu pertumbuhan tanaman. Bakteri endofit diisolasi dari bagian akar, batang, dan daun. Berdasarkan hasil isolasi yang telah dilakukan didapati 21 isolat bakteri endofit. Kemudian seleksi dilakukan dengan uji hipersensitif dan uji reaksi gram. Hasil seleksi diperoleh 20 isolat bakteri endofit yang bukan patogen. Setelah itu dilanjutkan dengan uji pemacu pertumbuhan tanaman terhadap isolat bakteri endofit. Hasil pengujian tersebut diperoleh tiga isolat bakteri yang berpotensi sebagai pemacu pertumbuhan tanaman yaitu STA1, STA6, dan STA11. Kata kunci: bakteri endofit, pemacu pertumbuhan tanaman, sagu

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Diversity and capacity to promote maize growth of bacteria isolated from the Amazon region

Acta Amazonica ◽

10.1590/1809-4392201502502 ◽

2016 ◽

Vol 46 (2) ◽

pp. 111-118 ◽

Cited By ~ 3

Author(s):

Krisle da SILVA ◽

Liamara PERIN ◽

Maria de Lourdes GOMES ◽

Alexandre Cardoso BARAÚNA ◽

Gilmara Maria Duarte PEREIRA ◽

...

Keyword(s):

Calcium Phosphate ◽

Plant Growth ◽

Endophytic Bacteria ◽

Nifh Gene ◽

Amazon Region ◽

Forest Area ◽

Plant Growth Promoting Bacteria ◽

Plant Growth Promoting ◽

Maize Plants ◽

Growth Promoting

ABSTRACT Maize plants can establish beneficial associations with plant growth-promoting bacteria. However, few studies have been conducted on the characterization and inoculation of these bacteria in the Amazon region. This study aimed to characterize endophytic bacteria isolated from maize in the Amazon region and to assess their capacity to promote plant growth. Fifty-five bacterial isolates were obtained from maize grown in two types of ecosystems, i.e., a cerrado (savanna) and a forest area. The isolates were characterized by the presence of the nifH gene, their ability to synthesize indole-3-acetic acid (IAA) and solubilize calcium phosphate (CaHPO4), and 16S rRNA partial gene sequencing. Twenty-four bacteria contained the nifH gene, of which seven were isolated from maize plants cultivated in a cerrado area and seventeen from a forest area. Fourteen samples showed the capacity to synthesize IAA and only four solubilized calcium phosphate. The following genera were found among these isolates: Pseudomonas; Acinetobacter; Enterobacter; Pantoea; Burkholderia and Bacillus. In addition, eight isolates with plant growth-promoting capacity were selected for a glasshouse experiment involving the inoculation of two maize genotypes (a hybrid and a variety) grown in pots containing soil. Inoculation promoted the development of the maize plants but no significant interaction between maize cultivar and bacterial inoculation was found. A high diversity of endophytic bacteria is present in the Amazon region and these bacteria have potential to promote the development of maize plants.

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Culturable endophytic bacteria from stems and leaves of garden pea (Pisum sativum L.)

Ecological genetics ◽

10.17816/ecogen17915 ◽

2020 ◽

Author(s):

Ekaterina N. Vasileva ◽

Gulnar A. Akhtemova ◽

Alexey M. Afonin ◽

Alexey Borisov ◽

Igor A. Tikhonovich ◽

...

Keyword(s):

Pisum Sativum ◽

Plant Growth ◽

Endophytic Bacteria ◽

Taxonomic Position ◽

Plant Growth Promoting Bacteria ◽

Garden Pea ◽

Pisum Sativum L ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Stems And Leaves

Background. Endophytic microorganisms inhabit internal tissues of most plants. However, little is known about endophytic community of the garden pea (Pisum sativum L.), an agriculturally important crop. Materials and methods. Culturable endophytic bacteria were isolated from sterilized stems and leaves of three pea genotypes: K-8274 (cv. Vendevil), K-3358 (unnamed cultivar), and cv.Triumph. The taxonomic position of isolates was determined by 16S rRNA gene sequencing. The plant growth-promoting capabilityof identified bacteria was tested on the roots of watercress (Lepidium sativum L.). Results. In total, out of 118 morphotypes of culturable endophytic bacteria identified, for 80 the taxonomic position was determined. Proteobacteria and Firmicuteswere dominant phyla, and Actinobacteria were present in minority. Eight bacterial isolates demonstrated the plant growth-promoting capability, and one of them KV17 (Rahnella sp.) maintained this capability after several passages and prolonged storage. Conclusion.The plant growth-promoting bacteria isolated from pea stems and leaves can become a component of microbiological preparations.

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Endophytic bacteria associated with tissue culture and leaves of Plinia peruviana

Pesquisa Agropecuária Brasileira ◽

10.1590/s1678-3921.pab2020.v55.01844 ◽

2020 ◽

Vol 55 ◽

Author(s):

Eder Gonçalves Queiroz ◽

Juliana Degenhardt ◽

Marguerite Quoirin ◽

Krisle da Silva

Keyword(s):

Tissue Culture ◽

Plant Growth ◽

Endophytic Bacteria ◽

Nodal Segments ◽

Rrna Gene ◽

Plant Growth Promoting Bacteria ◽

Bacillus Sp ◽

Indolic Compounds ◽

Plant Growth Promoting ◽

Growth Promoting

Abstract: The objective of this work was to isolate endophytic bacteria from tissue culture and leaves of jaboticaba (Plinia peruviana) and to evaluate their potential as plant growth-promoting bacteria. The bacteria were isolated from nodal segments grown in vitro and from leaves from a tree under natural conditions, totaling 11 and 54 isolates, respectively. The isolates were characterized by colony morphology. The indolic compounds produced by the isolates, in the presence or absence of 100 mg L-1 tryptophan, were quantified. The greatest producers of these compounds were identified by sequencing the 16S rRNA gene and were inoculated on jaboticaba seeds, using Azospirillum brasilense (Ab-V6) as a positive control. The sensitivity of bacteria to eight antibiotics was also evaluated. All assessed bacteria produced indolic compounds, especially Bacillus sp., with a content of 27.41 μg mL-1. The germination rate of the seeds inoculated with Stenotrophomonas sp. was high - 97.34% compared with that of 74.67% of the negative control. Bacillus sp. and Stenotrophomonas sp. also sped up germination. Chloramphenicol limited the growth of 82% of the isolates, followed by amoxicillin, gentamicin, levofloxacin, and tetracycline, which limited 70%; erythromycin was only effective against 35%. The endophytic bacteria isolated from jaboticaba show characteristics of plant growth-promoting bacteria, and Bacillus sp. and Stenotrophomonas sp., obtained from tissue culture, are capable of enhancing jaboticaba seed germination.

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Plant growth‐promoting activity of endophytic bacteria from sweet sorghum (Sorghum bicolor (L.) Moench)

Indonesian Journal of Biotechnology ◽

10.22146/ijbiotech.64893 ◽

2021 ◽

Vol 26 (4) ◽

pp. 190

Author(s):

Charlie Ester De Fretes ◽

Donny Widianto ◽

Yekti Asih Purwestri ◽

Tri Rini Nuringtyas

Keyword(s):

Plant Growth ◽

Sweet Sorghum ◽

Endophytic Bacteria ◽

Environmental Damage ◽

Plant Growth Promoting Bacteria ◽

Bacillus Sp ◽

Chemical Fertilizers ◽

Paenibacillus Sp ◽

Plant Growth Promoting ◽

Growth Promoting

Application of high levels of chemical fertilizers for optimal growth of sweet sorghum causes environmental degradation. Plant growth‐promoting bacteria have biotechnological importance because they can improve the growth and health of important agronomic plants. This study aimed to isolate, characterize, and identify endophytic bacteria associated with sweet sorghum (cv. KCS105), and also to study the inoculation effects of selected isolates on sorghum growth. In this study, 35 isolates were evaluated for their ability to support plant growth. The results showed that seven isolates were diazotrophic, six were capable of dissolving phosphate, six produced IAA and could detect ACC‐deaminase activity, and three inhibited the growth of pathogenic fungi. Nine isolates exhibiting mechanisms for promoting plant growth from the Alphaproteobacteria (Devosia), Firmicutes (Bacillus, Paenibacillus, Staphylococcus), and Actinobacteria (Microbacterium, Brachybacterium) phyla were identified. In addition, the Paenibacillus sp. BB7, Bacillus sp. PIB1B, and Bacillus sp. PLB1B isolates showed increasing effects on plant growth in greenhouse tests. Endophytic bacterial isolates which display plant growth‐promoting features can potentially be employed as biofertilizer agents. They may also address environmental damage problems resulting from the use of chemical fertilizers and pesticides.

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ENDOPHYTIC BACTERIA USED AS BIOINOCULANTS IN MICROPROPAGATED BANANA SEEDLINGS

Revista Brasileira de Fruticultura ◽

10.1590/0100-29452017324 ◽

2017 ◽

Vol 39 (2) ◽

Author(s):

GLEIKA LARISSE OLIVEIRA DORASIO DE SOUZA ◽

DEIVISSON FERREIRA DA SILVA ◽

SILVIA NIETSCHE ◽

ADELICA APARECIDA XAVIER ◽

MARLON CRISTIAN TOLEDO PEREIRA

Keyword(s):

Plant Growth ◽

Endophytic Bacteria ◽

Plant Growth Promoting Bacteria ◽

Bacillus Sp ◽

Microbial Inoculants ◽

Plant Growth Promoting ◽

Promote Plant Growth ◽

Growth Promoting ◽

Indole 3 Acetic Acid

ABSTRACT The use of bio-fertilizers and microbial inoculants that promote plant growth and increased yield has been accepted as an alternative to reduce the use of chemical fertilizers. The objective of the present study was to evaluate the effect of plant growth promoting bacteria inoculation on growth and quality of micropropagated banana seedlings ‘Prata Anã’. The experimental design was completely randomized, with four replications and the treatments consisted of 24 isolates of endophytic bacteria. The isolates EB-50 (Bacillus sp.) and EB-133 (Bacillus amyloliquefaciens) characterized as diazotrophic, the EB-51 (Bacillus pumilus) indicate for inorganic phosphate solubilization and EB-55(Bacillus subtilis) and EB-40 (Bacillus sp.) indole-3-acetic acid producers have provided significant increases for length, pseudostem diameter, fresh masses and dry masses in ‘Prata Anã’ micropropagated banana seedlings.

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Mediterranean Native Leguminous Plants: A Reservoir of Endophytic Bacteria with Potential to Enhance Chickpea Growth under Stress Conditions

Microorganisms ◽

10.3390/microorganisms7100392 ◽

2019 ◽

Vol 7 (10) ◽

pp. 392 ◽

Cited By ~ 2

Author(s):

Clarisse Brígido ◽

Esther Menéndez ◽

Ana Paço ◽

Bernard R. Glick ◽

Anabela Belo ◽

...

Keyword(s):

Plant Growth ◽

Endophytic Bacteria ◽

Total Biomass ◽

Plant Growth Promoting Bacteria ◽

Legume Species ◽

Bacterial Endophytes ◽

Acetic Acid Production ◽

Plant Growth Promoting ◽

Native Legume ◽

Growth Promoting

Bacterial endophytes, a subset of a plant’s microbiota, can facilitate plant growth by a number of different mechanisms. The aims of this study were to assess the diversity and functionality of endophytic bacterial strains from internal root tissues of native legume species grown in two distinct sites in South of Portugal and to evaluate their ability to promote plant growth. Here, 122 endophytic bacterial isolates were obtained from 12 different native legume species. Most of these bacteria possess at least one of the plant growth-promoting features tested in vitro, with indole acetic acid production being the most common feature among the isolates followed by the production of siderophores and inorganic phosphate solubilization. The results of in planta experiments revealed that co-inoculation of chickpea plants with specific endophytic bacteria along with N2-fixing symbionts significantly improved the total biomass of chickpea plants, in particular when these plants were grown under saline conditions. Altogether, this study revealed that Mediterranean native legume species are a reservoir of plant growth-promoting bacteria, that are also tolerant to salinity and to toxic levels of Mn. Thus, these bacterial endophytes are well adapted to common constraints present in soils of this region which constitutes important factors to consider in the development of bacterial inoculants for stressful conditions in the Mediterranean region.

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Characterization & identification endophytic bacteria from Sorghum bicolor (L.) Moench as plant growth promoting bacteria

International Journal of Scientific and Engineering Research ◽

10.14299/ijser.2019.07.09 ◽

2019 ◽

Vol 10 (7) ◽

pp. 929-933

Author(s):

Charlie Ester de Fretes ◽

Donny Widianto ◽

Yekti Asih Purwestri ◽

Tri Rini Nuringtyas

Keyword(s):

Plant Growth ◽

Sorghum Bicolor ◽

Endophytic Bacteria ◽

Plant Growth Promoting Bacteria ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Sorghum Bicolor L

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Characterization and Diversity of plant growth promoting Endophytic and Rhizosphere Bacteria isolated from theRhizosphere and tissues of Pepper and their effect on plant growth promotion and disease suppression of Phytophthora capsici

10.21203/rs.3.rs-422729/v1 ◽

2021 ◽

Author(s):

Mesele Admassie ◽

Yitbark Wold-Hawariat ◽

Tesefaye Alemu

Keyword(s):

Acetic Acid ◽

Plant Growth ◽

Endophytic Bacteria ◽

Phytophthora Capsici ◽

Plant Diseases ◽

Plant Growth Promoting Bacteria ◽

Plant Growth Promoting ◽

Promote Plant Growth ◽

Growth Promoting ◽

Pepper Plants

Abstract Plant growth promoting rhizo and endophytic bacteria were isolated from different parts of pepper from south eastern Ethiopia. Plant growth promoting bacteria (PGP) are those that may be used to promote plant growth and suppress plant diseases. The objectives of this study were to identify and characterize PGPB indigenous to pepper rhizosphere and endophyte bacteria in Ethiopia, and to determine their capacity to suppress Phytophthora capsici in pepper. From a total of 60 isolates, 20 were selected based on their in vitro antagonism activity of phytopathogens and plant growth promoting traits. From the total 60 strains representing, 38 rhizosphere, and 22 endophytic bacteria were identified based on biochemical assays of semi-automated Vitec 2 compact and ten potential bacteria further identified by molecular methods. Results revealed that only one isolate of rhizosphere and three endophytic bacteria showed more than 50% suppression of test pathogens. The isolates were evaluated for their ability to solubilize phosphate, as well as for ammonia, indole acetic acid, hydrogen cyanide, and biofilm production. The selected isolates produced (0.2–93 µg mL− 1) of indole-3-acetic acid, without supplemented with tryptophan, while supplemented with tryptophan it produces (11.23–159 µg mL− 1). The activities of plant growth-promoting were assessed by measuring their effect on the number of lateral roots, root and shoot length of Arabidopsis plants, and germination percentage of pepper plants. Pepper plants grown from seeds that were treated with these PGPB strains showed significantly higher levels of germination, seedling vigor, and growth, compared to non-treated control plants. Since these PGPB inoculants showed multiple characters useful to the host plants, they may be used as an alternative in the production of new, safe, and effective seed treatments as bio-fungicides. Generally, this work exhibits the potential of bacterial isolates to control Phytophthora infection and promote plant growth.

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