scholarly journals Culturable endophytic bacteria from stems and leaves of garden pea (Pisum sativum L.)

2020 ◽  
Author(s):  
Ekaterina N. Vasileva ◽  
Gulnar A. Akhtemova ◽  
Alexey M. Afonin ◽  
Alexey Borisov ◽  
Igor A. Tikhonovich ◽  
...  
Keyword(s):  
Pisum Sativum ◽  
Plant Growth ◽  
Endophytic Bacteria ◽  
Taxonomic Position ◽  
Plant Growth Promoting Bacteria ◽  
Garden Pea ◽  
Pisum Sativum L ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Stems And Leaves

Background. Endophytic microorganisms inhabit internal tissues of most plants. However, little is known about endophytic community of the garden pea (Pisum sativum L.), an agriculturally important crop. Materials and methods. Culturable endophytic bacteria were isolated from sterilized stems and leaves of three pea genotypes: K-8274 (cv. Vendevil), K-3358 (unnamed cultivar), and cv.Triumph. The taxonomic position of isolates was determined by 16S rRNA gene sequencing. The plant growth-promoting capabilityof identified bacteria was tested on the roots of watercress (Lepidium sativum L.). Results. In total, out of 118 morphotypes of culturable endophytic bacteria identified, for 80 the taxonomic position was determined. Proteobacteria and Firmicuteswere dominant phyla, and Actinobacteria were present in minority. Eight bacterial isolates demonstrated the plant growth-promoting capability, and one of them KV17 (Rahnella sp.) maintained this capability after several passages and prolonged storage. Conclusion.The plant growth-promoting bacteria isolated from pea stems and leaves can become a component of microbiological preparations.

scholarly journals Potensi Bakteri Endofit dari Tanaman Sagu (Metroxylon spp.) sebagai Agens Pemacu Pertumbuhan Tanaman Padi

2018 ◽  
Vol 14 (2) ◽  
pp. 75-80
Author(s):  
Yatni Yatni ◽  
Gratiana N C Tuhumury ◽  
Christoffol Leiwakabessy
Keyword(s):  
Plant Growth ◽  
Endophytic Bacteria ◽  
Growth Promoter ◽  
Plant Growth Promoting Bacteria ◽  
Test Results ◽  
Bacterial Isolates ◽  
The People ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Stems And Leaves

Sago is a staple plant of the people of Maluku and Papua, which have many benefits and advantages to continue to be developed. Endophytic bacteria are bacteria that live in plant tissues and colonize the intercellular and vascular systems. This study aims to obtain endophytic bacterial isolates from parts of the roots, stems, and leaves of sago plants which have the potential as agents for plant growth promoting bacteria. Endophytic bacteria are isolated from the roots, stems, and leaves. Based on the results of isolation, 21 isolates of endophytic bacteria were found. Then, the selection is done by hypersensitivity test and gram reaction test. The results of the selection obtained 20 isolates of endophytic bacteria that were not pathogenic. After that, it was followed by plant growth promoting test for endophytic bacterial isolates. The test results were obtained three isolates potentially as plant growth promoter that is STA1, STA6, and STA11. Keywords: endophytic bacteria, plant growth promoting, sago   ABSTRAK Tanaman sagu merupakan tanaman pokok masyarakat Maluku dan Papua, yang memiliki banyak manfaat dan keunggulan untuk terus dikembangkan. Bakteri endofit adalah bakteri yang hidup di dalam jaringan tanaman dan berkoloni pada daerah ruang interseluler dan sistem vascular. Penelitian ini bertujuan untuk mendapatkan isolat bakteri endofit dari bagian akar,batang dan daun tanaman sagu yang berpotensi sebagai agens pemacu pertumbuhan tanaman. Bakteri endofit diisolasi dari bagian akar, batang, dan daun. Berdasarkan hasil isolasi yang telah dilakukan didapati 21 isolat bakteri endofit. Kemudian seleksi dilakukan dengan uji hipersensitif dan uji reaksi gram. Hasil seleksi diperoleh 20 isolat bakteri endofit yang bukan patogen. Setelah itu dilanjutkan dengan uji pemacu pertumbuhan tanaman terhadap isolat bakteri endofit. Hasil pengujian tersebut diperoleh tiga isolat bakteri yang berpotensi sebagai pemacu pertumbuhan tanaman yaitu STA1, STA6, dan STA11. Kata kunci: bakteri endofit, pemacu pertumbuhan tanaman, sagu

Isolasi dan Karakterisasi Bakteri Endofit Pelarut Fosfat dan Penghasil Hidrogen Cyanide (HCN) dari Tanaman Bawang Merah (Allium cepa L).

2018 ◽  
Vol 20 (1) ◽  
pp. 9
Author(s):  
Ryan Hilda Wandita ◽  
Sri Pujiyanto ◽  
Agung Suprihadi ◽  
Ratih Dewi Hastuti
Keyword(s):  
Plant Growth ◽  
Traditional Medicine ◽  
Allium Cepa ◽  
Endophytic Bacteria ◽  
Economic Value ◽  
Average Diameter ◽  
Plant Growth Promoting Bacteria ◽  
Allium Cepa L ◽  
Plant Growth Promoting ◽  
Growth Promoting

Onions (Allium cepa L.) is one of the leading horticultural commodities in Indonesia and is often used as seasoning and traditional medicine. Onion has a high economic value and fluctuating prices so that domestic onion production needs to be improved, one of them with a presence of endophytic bacteria that act as plant growth promoting agent or Plant Growth Promoting Bacteria (PGPB). Endophytic bacteria isolated from the root, leaves, and bulbs. In this research has been tested endophytic bacteria of onion plants from Garut regency which has PGPB factors such as able to dissolve phosphate, and produce HCN. The results obtained 251 isolates of endophytic bacteria. Based on the characterization results, the superior isolates capable of dissolving phosphate with an average diameter of 0.45 cm is isolate II.B.1D.3, and 11 isolates capable of producing high HCN. These isolates can be used as PGPB agents so that they can be useful in increasing plant growth and onion production and biocontrol in suppressing pathogens. Keywords: PGPB, endophyte, onion, phosphate, HCN

scholarly journals Endophytic bacteria isolated from garden pea (Pisum sativum L.)

2020 ◽  
Author(s):  
E. N. Vasileva ◽  
A. M. Afonin ◽  
G. A. Akhtemova ◽  
V. A. Zhukov ◽  
I. A. Tikhonovich
Keyword(s):  
16S Rrna ◽  
Pisum Sativum ◽  
16S Rrna Gene ◽  
Endophytic Bacteria ◽  
Taxonomic Status ◽  
Rrna Gene ◽  
Garden Pea ◽  
Aerial Parts ◽  
Pisum Sativum L ◽  
Growth Promoting

Endophytic bacteria were isolated from surface-sterilized aerial parts of pea. Taxonomic status of isolated strains was determined by sequencing of 16S rRNA gene. Moreover, genomes of growth-promoting endophytes were sequenced.

scholarly journals Diversity and capacity to promote maize growth of bacteria isolated from the Amazon region

2016 ◽  
Vol 46 (2) ◽  
pp. 111-118 ◽  
Author(s):  
Krisle da SILVA ◽  
Liamara PERIN ◽  
Maria de Lourdes GOMES ◽  
Alexandre Cardoso BARAÚNA ◽  
Gilmara Maria Duarte PEREIRA ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Plant Growth ◽  
Endophytic Bacteria ◽  
Nifh Gene ◽  
Amazon Region ◽  
Forest Area ◽  
Plant Growth Promoting Bacteria ◽  
Plant Growth Promoting ◽  
Maize Plants ◽  
Growth Promoting

ABSTRACT Maize plants can establish beneficial associations with plant growth-promoting bacteria. However, few studies have been conducted on the characterization and inoculation of these bacteria in the Amazon region. This study aimed to characterize endophytic bacteria isolated from maize in the Amazon region and to assess their capacity to promote plant growth. Fifty-five bacterial isolates were obtained from maize grown in two types of ecosystems, i.e., a cerrado (savanna) and a forest area. The isolates were characterized by the presence of the nifH gene, their ability to synthesize indole-3-acetic acid (IAA) and solubilize calcium phosphate (CaHPO4), and 16S rRNA partial gene sequencing. Twenty-four bacteria contained the nifH gene, of which seven were isolated from maize plants cultivated in a cerrado area and seventeen from a forest area. Fourteen samples showed the capacity to synthesize IAA and only four solubilized calcium phosphate. The following genera were found among these isolates: Pseudomonas; Acinetobacter; Enterobacter; Pantoea; Burkholderia and Bacillus. In addition, eight isolates with plant growth-promoting capacity were selected for a glasshouse experiment involving the inoculation of two maize genotypes (a hybrid and a variety) grown in pots containing soil. Inoculation promoted the development of the maize plants but no significant interaction between maize cultivar and bacterial inoculation was found. A high diversity of endophytic bacteria is present in the Amazon region and these bacteria have potential to promote the development of maize plants.

Plant growth promoting rhizobacteria from heavy metal contaminated soil promote growth attributes of Pisum sativum L.

2019 ◽  
Vol 17 ◽  
pp. 665-671 ◽  
Author(s):  
Simranjeet Singh ◽  
Vijay Kumar ◽  
Gurpreet Kaur Sidhu ◽  
Shivika Datta ◽  
Daljeet Singh Dhanjal ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Pisum Sativum ◽  
Plant Growth ◽  
Contaminated Soil ◽  
Plant Growth Promoting Rhizobacteria ◽  
Pisum Sativum L ◽  
Plant Growth Promoting ◽  
Growth Attributes ◽  
Growth Promoting
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