scholarly journals Endophytic bacteria associated with tissue culture and leaves of Plinia peruviana

2020 ◽  
Vol 55 ◽  
Author(s):  
Eder Gonçalves Queiroz ◽  
Juliana Degenhardt ◽  
Marguerite Quoirin ◽  
Krisle da Silva
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Endophytic Bacteria ◽  
Nodal Segments ◽  
Rrna Gene ◽  
Plant Growth Promoting Bacteria ◽  
Bacillus Sp ◽  
Indolic Compounds ◽  
Plant Growth Promoting ◽  
Growth Promoting

Abstract: The objective of this work was to isolate endophytic bacteria from tissue culture and leaves of jaboticaba (Plinia peruviana) and to evaluate their potential as plant growth-promoting bacteria. The bacteria were isolated from nodal segments grown in vitro and from leaves from a tree under natural conditions, totaling 11 and 54 isolates, respectively. The isolates were characterized by colony morphology. The indolic compounds produced by the isolates, in the presence or absence of 100 mg L-1 tryptophan, were quantified. The greatest producers of these compounds were identified by sequencing the 16S rRNA gene and were inoculated on jaboticaba seeds, using Azospirillum brasilense (Ab-V6) as a positive control. The sensitivity of bacteria to eight antibiotics was also evaluated. All assessed bacteria produced indolic compounds, especially Bacillus sp., with a content of 27.41 μg mL-1. The germination rate of the seeds inoculated with Stenotrophomonas sp. was high - 97.34% compared with that of 74.67% of the negative control. Bacillus sp. and Stenotrophomonas sp. also sped up germination. Chloramphenicol limited the growth of 82% of the isolates, followed by amoxicillin, gentamicin, levofloxacin, and tetracycline, which limited 70%; erythromycin was only effective against 35%. The endophytic bacteria isolated from jaboticaba show characteristics of plant growth-promoting bacteria, and Bacillus sp. and Stenotrophomonas sp., obtained from tissue culture, are capable of enhancing jaboticaba seed germination.

scholarly journals Plant growth‐promoting activity of endophytic bacteria from sweet sorghum (Sorghum bicolor (L.) Moench)

2021 ◽  
Vol 26 (4) ◽  
pp. 190
Author(s):  
Charlie Ester De Fretes ◽  
Donny Widianto ◽  
Yekti Asih Purwestri ◽  
Tri Rini Nuringtyas
Keyword(s):  
Plant Growth ◽  
Sweet Sorghum ◽  
Endophytic Bacteria ◽  
Environmental Damage ◽  
Plant Growth Promoting Bacteria ◽  
Bacillus Sp ◽  
Chemical Fertilizers ◽  
Paenibacillus Sp ◽  
Plant Growth Promoting ◽  
Growth Promoting

Application of high levels of chemical fertilizers for optimal growth of sweet sorghum causes environmental degradation. Plant growth‐promoting bacteria have biotechnological importance because they can improve the growth and health of important agronomic plants. This study aimed to isolate, characterize, and identify endophytic bacteria associated with sweet sorghum (cv. KCS105), and also to study the inoculation effects of selected isolates on sorghum growth. In this study, 35 isolates were evaluated for their ability to support plant growth. The results showed that seven isolates were diazotrophic, six were capable of dissolving phosphate, six produced IAA and could detect ACC‐deaminase activity, and three inhibited the growth of pathogenic fungi. Nine isolates exhibiting mechanisms for promoting plant growth from the Alphaproteobacteria (Devosia), Firmicutes (Bacillus, Paenibacillus, Staphylococcus), and Actinobacteria (Microbacterium, Brachybacterium) phyla were identified. In addition, the Paenibacillus sp. BB7, Bacillus sp. PIB1B, and Bacillus sp. PLB1B isolates showed increasing effects on plant growth in greenhouse tests. Endophytic bacterial isolates which display plant growth‐promoting features can potentially be employed as biofertilizer agents. They may also address environmental damage problems resulting from the use of chemical fertilizers and pesticides.

scholarly journals ENDOPHYTIC BACTERIA USED AS BIOINOCULANTS IN MICROPROPAGATED BANANA SEEDLINGS

2017 ◽  
Vol 39 (2) ◽  
Author(s):  
GLEIKA LARISSE OLIVEIRA DORASIO DE SOUZA ◽  
DEIVISSON FERREIRA DA SILVA ◽  
SILVIA NIETSCHE ◽  
ADELICA APARECIDA XAVIER ◽  
MARLON CRISTIAN TOLEDO PEREIRA
Keyword(s):  
Plant Growth ◽  
Endophytic Bacteria ◽  
Plant Growth Promoting Bacteria ◽  
Bacillus Sp ◽  
Microbial Inoculants ◽  
Plant Growth Promoting ◽  
Promote Plant Growth ◽  
Growth Promoting ◽  
Indole 3 Acetic Acid

ABSTRACT The use of bio-fertilizers and microbial inoculants that promote plant growth and increased yield has been accepted as an alternative to reduce the use of chemical fertilizers. The objective of the present study was to evaluate the effect of plant growth promoting bacteria inoculation on growth and quality of micropropagated banana seedlings ‘Prata Anã’. The experimental design was completely randomized, with four replications and the treatments consisted of 24 isolates of endophytic bacteria. The isolates EB-50 (Bacillus sp.) and EB-133 (Bacillus amyloliquefaciens) characterized as diazotrophic, the EB-51 (Bacillus pumilus) indicate for inorganic phosphate solubilization and EB-55(Bacillus subtilis) and EB-40 (Bacillus sp.) indole-3-acetic acid producers have provided significant increases for length, pseudostem diameter, fresh masses and dry masses in ‘Prata Anã’ micropropagated banana seedlings.

Assessing the Plant Growth-promoting Traits and Host Specificity of Endophytic Bacteria of Pulse Crops

2021 ◽  
Author(s):  
R. Thamizh Vendan ◽  
D. Balachandar
Keyword(s):  
Plant Growth ◽  
Endophytic Bacteria ◽  
Gene Sequencing ◽  
16S Rrna Gene Sequencing ◽  
Growth And Yield ◽  
Rrna Gene ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Rrna Gene Sequencing ◽  
Plant Growth Promoting Traits

Background: Symbiotic associations between legumes and Rhizobia are ancient and fundamental. However, the plant growth-promoting endophytes other than Rhizobia are not yet fully explored for pulses productivity. The present study was aimed to isolate efficient endophytic bacteria from pulses, assess their diversity, screen their plant growth-promoting activities and to test their potential as bio inoculants for pulses.Methods: We have isolated several endophytic bacteria from pulse crops more specifically from blackgram (Vigna mungo) and greengram (Vigna radiata). After careful screening, 15 promising endophytic isolates were selected for this study. The identification of endophytic bacterial isolates was performed by 16S rRNA gene sequencing. The isolates were tested for their potential for the plant growth-promoting traits such as nitrogen fixation, phosphate solubilization, indole-3-acetic acid production, siderophore secretion and antifungal activity. Pot culture experiments were conducted with the screened potential endophytic cultures.Result: The 16S rRNA gene sequencing revealed that species of Enterobacter, Bacillus, Pantoea, Pseudomonas, Acromobacter, Ocrobacterium were found as endophytes in blackgram and greengram. The in vitro screening identified Bacillus pumilus (BG-E6), Pseudomonas fluorescens (BG-E5) and Bacillus licheniformis (BG-E3) from blackgram and Pseudomonas chlororaphis (GG-E2) and Bacillus thuringiensis (GG-E7) from greengram as potential plant growth-promoting endophytes. These strains showed antagonism against plant pathogenic fungi. Upon inoculation of these endophytic PGPR strains, the blackgram and greengram growth and yield got increased. Among the strains, BG-E6 recorded 14.7% increased yield in blackgram and GG-E2 accounted for a 19.5% yield increase in greengram compared to respective uninoculated control. The experimental results showed that there was a host specificity found among the endophytic bacterial cultures with pulses. The cross inoculation of endophytic strains did not perform well to enhance the growth and yield of their alternate hosts. 

Isolasi dan Karakterisasi Bakteri Endofit Pelarut Fosfat dan Penghasil Hidrogen Cyanide (HCN) dari Tanaman Bawang Merah (Allium cepa L).

2018 ◽  
Vol 20 (1) ◽  
pp. 9
Author(s):  
Ryan Hilda Wandita ◽  
Sri Pujiyanto ◽  
Agung Suprihadi ◽  
Ratih Dewi Hastuti
Keyword(s):  
Plant Growth ◽  
Traditional Medicine ◽  
Allium Cepa ◽  
Endophytic Bacteria ◽  
Economic Value ◽  
Average Diameter ◽  
Plant Growth Promoting Bacteria ◽  
Allium Cepa L ◽  
Plant Growth Promoting ◽  
Growth Promoting

Onions (Allium cepa L.) is one of the leading horticultural commodities in Indonesia and is often used as seasoning and traditional medicine. Onion has a high economic value and fluctuating prices so that domestic onion production needs to be improved, one of them with a presence of endophytic bacteria that act as plant growth promoting agent or Plant Growth Promoting Bacteria (PGPB). Endophytic bacteria isolated from the root, leaves, and bulbs. In this research has been tested endophytic bacteria of onion plants from Garut regency which has PGPB factors such as able to dissolve phosphate, and produce HCN. The results obtained 251 isolates of endophytic bacteria. Based on the characterization results, the superior isolates capable of dissolving phosphate with an average diameter of 0.45 cm is isolate II.B.1D.3, and 11 isolates capable of producing high HCN. These isolates can be used as PGPB agents so that they can be useful in increasing plant growth and onion production and biocontrol in suppressing pathogens. Keywords: PGPB, endophyte, onion, phosphate, HCN

scholarly journals Effect of Pyroligneous Acid on the Microbial Community Composition and Plant Growth-Promoting Bacteria (PGPB) in Soils

Soil Systems ◽  
2022 ◽  
Vol 6 (1) ◽  
pp. 10
Author(s):  
Anithadevi Kenday Sivaram ◽  
Logeshwaran Panneerselvan ◽  
Kannappar Mukunthan ◽  
Mallavarapu Megharaj
Keyword(s):  
Microbial Community ◽  
Plant Growth ◽  
Microbial Diversity ◽  
Growth And Yield ◽  
Rrna Gene ◽  
Plant Growth Promoting Bacteria ◽  
Microbial Composition ◽  
Pyroligneous Acid ◽  
Plant Growth Promoting ◽  
Growth Promoting

Pyroligneous acid (PA) is often used in agriculture as a plant growth and yield enhancer. However, the influence of PA application on soil microorganisms is not often studied. Therefore, in this study, we investigated the effect of PA (0.01–5% w/w in soil) on the microbial diversity in two different soils. At the end of eight weeks of incubation, soil microbial community dynamics were determined by Illumina-MiSeq sequencing of 16S rRNA gene amplicons. The microbial composition differed between the lower (0.01% and 0.1%) and the higher (1% and 5%) concentration in both PA spiked soils. The lower concentration of PA resulted in higher microbial diversity and dehydrogenase activity (DHA) compared to the un-spiked control and the soil spiked with high PA concentrations. Interestingly, PA-induced plant growth-promoting bacterial (PGPB) genera include Bradyrhizobium, Azospirillum, Pseudomonas, Mesorhizobium, Rhizobium, Herbaspiriluum, Acetobacter, Beijerinckia, and Nitrosomonas at lower concentrations. Additionally, the PICRUSt functional analysis revealed the predominance of metabolism as the functional module’s primary component in both soils spiked with 0.01% and 0.1% PA. Overall, the results elucidated that PA application in soil at lower concentrations promoted soil DHA and microbial enrichment, particularly the PGPB genera, and thus have great implications for improving soil health.

scholarly journals Alone Yet Not Alone: Frankia Lives Under the Same Roof With Other Bacteria in Actinorhizal Nodules

2021 ◽  
Vol 12 ◽  
Author(s):  
Faten Ghodhbane-Gtari ◽  
Timothy D’Angelo ◽  
Abdellatif Gueddou ◽  
Sabrine Ghazouani ◽  
Maher Gtari ◽  
...  
Keyword(s):  
16S Rrna ◽  
Plant Growth ◽  
16S Rrna Gene ◽  
Root Nodules ◽  
Actinorhizal Plants ◽  
Rrna Gene ◽  
Plant Growth Promoting Bacteria ◽  
Metagenomic Sequencing ◽  
Plant Growth Promoting ◽  
Growth Promoting

Actinorhizal plants host mutualistic symbionts of the nitrogen-fixing actinobacterial genus Frankia within nodule structures formed on their roots. Several plant-growth-promoting bacteria have also been isolated from actinorhizal root nodules, but little is known about them. We were interested investigating the in planta microbial community composition of actinorhizal root nodules using culture-independent techniques. To address this knowledge gap, 16S rRNA gene amplicon and shotgun metagenomic sequencing was performed on DNA from the nodules of Casuarina glauca. DNA was extracted from C. glauca nodules collected in three different sampling sites in Tunisia, along a gradient of aridity ranging from humid to arid. Sequencing libraries were prepared using Illumina NextEra technology and the Illumina HiSeq 2500 platform. Genome bins extracted from the metagenome were taxonomically and functionally profiled. Community structure based off preliminary 16S rRNA gene amplicon data was analyzed via the QIIME pipeline. Reconstructed genomes were comprised of members of Frankia, Micromonospora, Bacillus, Paenibacillus, Phyllobacterium, and Afipia. Frankia dominated the nodule community at the humid sampling site, while the absolute and relative prevalence of Frankia decreased at the semi-arid and arid sampling locations. Actinorhizal plants harbor similar non-Frankia plant-growth-promoting-bacteria as legumes and other plants. The data suggests that the prevalence of Frankia in the nodule community is influenced by environmental factors, with being less abundant under more arid environments.

scholarly journals Effects of the Biofertilizer OYK (Bacillus sp.) Inoculation on Endophytic Microbial Community in Sweet Potato

Horticulturae ◽  
2020 ◽  
Vol 6 (4) ◽  
pp. 81
Author(s):  
Ahsanul Salehin ◽  
Md Hafizur Rahman Hafiz ◽  
Shohei Hayashi ◽  
Fumihiko Adachi ◽  
Kazuhito Itoh
Keyword(s):  
Plant Growth ◽  
Sweet Potato ◽  
Bacterial Communities ◽  
Ipomoea Batatas ◽  
Diversity Index ◽  
Plant Growth Promoting Bacteria ◽  
Bacillus Sp ◽  
Bacterial Composition ◽  
Plant Growth Promoting ◽  
Growth Promoting

Sweet potato (Ipomoea batatas L.) grows well even in infertile and nitrogen-limited fields, and endophytic bacterial communities have been proposed to be responsible for this ability. Plant-growth-promoting bacteria are considered eco-friendly and are used in agriculture, but their application can interact with endophytic communities in many ways. In this study, a commercial biofertilizer, OYK, consisting of a Bacillus sp., was applied to two cultivars of sweet potato, and the effects on indigenous endophytic bacterial communities in field conditions were examined. A total of 101 bacteria belonging to 25 genera in 9 classes were isolated. Although the inoculated OYK was not detected and significant plant-growth-promoting effects were not observed, the inoculation changed the endophytic bacterial composition, and the changes differed between the cultivars, as follows: Novosphingobium in α-Proteobacteria was dominant; it remained dominant in Beniharuka after the inoculation of OYK, while it disappeared in Beniazuma, with an increase in Sphingomonas and Sphingobium in α-Proteobacteria as well as Chryseobacterium and Acinetobacter in Flavobacteria. The behavior of Bacilli and Actinobacteria also differed between the cultivars. The Shannon diversity index (H) increased after inoculation in all conditions, and the values were similar between the cultivars. Competition of the inoculant with indigenous rhizobacteria and endophytes may determine the fates of the inoculant and the endophytic community.

Inoculation of plant growth-promoting bacteria Bacillus sp. YM-1 alleviates the toxicity of Pb to pakchoi

2018 ◽  
Vol 25 (28) ◽  
pp. 28216-28225 ◽  
Author(s):  
Sumei Yu ◽  
Jinsong Liang ◽  
Xin Bai ◽  
Liying Dong ◽  
Xuesheng Liu ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Promoting Bacteria ◽  
Bacillus Sp ◽  
Plant Growth Promoting ◽  
Growth Promoting

scholarly journals Potensi Bakteri Endofit dari Tanaman Sagu (Metroxylon spp.) sebagai Agens Pemacu Pertumbuhan Tanaman Padi

2018 ◽  
Vol 14 (2) ◽  
pp. 75-80
Author(s):  
Yatni Yatni ◽  
Gratiana N C Tuhumury ◽  
Christoffol Leiwakabessy
Keyword(s):  
Plant Growth ◽  
Endophytic Bacteria ◽  
Growth Promoter ◽  
Plant Growth Promoting Bacteria ◽  
Test Results ◽  
Bacterial Isolates ◽  
The People ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Stems And Leaves

Sago is a staple plant of the people of Maluku and Papua, which have many benefits and advantages to continue to be developed. Endophytic bacteria are bacteria that live in plant tissues and colonize the intercellular and vascular systems. This study aims to obtain endophytic bacterial isolates from parts of the roots, stems, and leaves of sago plants which have the potential as agents for plant growth promoting bacteria. Endophytic bacteria are isolated from the roots, stems, and leaves. Based on the results of isolation, 21 isolates of endophytic bacteria were found. Then, the selection is done by hypersensitivity test and gram reaction test. The results of the selection obtained 20 isolates of endophytic bacteria that were not pathogenic. After that, it was followed by plant growth promoting test for endophytic bacterial isolates. The test results were obtained three isolates potentially as plant growth promoter that is STA1, STA6, and STA11. Keywords: endophytic bacteria, plant growth promoting, sago   ABSTRAK Tanaman sagu merupakan tanaman pokok masyarakat Maluku dan Papua, yang memiliki banyak manfaat dan keunggulan untuk terus dikembangkan. Bakteri endofit adalah bakteri yang hidup di dalam jaringan tanaman dan berkoloni pada daerah ruang interseluler dan sistem vascular. Penelitian ini bertujuan untuk mendapatkan isolat bakteri endofit dari bagian akar,batang dan daun tanaman sagu yang berpotensi sebagai agens pemacu pertumbuhan tanaman. Bakteri endofit diisolasi dari bagian akar, batang, dan daun. Berdasarkan hasil isolasi yang telah dilakukan didapati 21 isolat bakteri endofit. Kemudian seleksi dilakukan dengan uji hipersensitif dan uji reaksi gram. Hasil seleksi diperoleh 20 isolat bakteri endofit yang bukan patogen. Setelah itu dilanjutkan dengan uji pemacu pertumbuhan tanaman terhadap isolat bakteri endofit. Hasil pengujian tersebut diperoleh tiga isolat bakteri yang berpotensi sebagai pemacu pertumbuhan tanaman yaitu STA1, STA6, dan STA11. Kata kunci: bakteri endofit, pemacu pertumbuhan tanaman, sagu

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