Isolasi dan Karakterisasi Bakteri Endofit Pelarut Fosfat dan Penghasil Hidrogen Cyanide (HCN) dari Tanaman Bawang Merah (Allium cepa L).

2018 ◽  
Vol 20 (1) ◽  
pp. 9
Author(s):  
Ryan Hilda Wandita ◽  
Sri Pujiyanto ◽  
Agung Suprihadi ◽  
Ratih Dewi Hastuti
Keyword(s):  
Plant Growth ◽  
Traditional Medicine ◽  
Allium Cepa ◽  
Endophytic Bacteria ◽  
Economic Value ◽  
Average Diameter ◽  
Plant Growth Promoting Bacteria ◽  
Allium Cepa L ◽  
Plant Growth Promoting ◽  
Growth Promoting

Onions (Allium cepa L.) is one of the leading horticultural commodities in Indonesia and is often used as seasoning and traditional medicine. Onion has a high economic value and fluctuating prices so that domestic onion production needs to be improved, one of them with a presence of endophytic bacteria that act as plant growth promoting agent or Plant Growth Promoting Bacteria (PGPB). Endophytic bacteria isolated from the root, leaves, and bulbs. In this research has been tested endophytic bacteria of onion plants from Garut regency which has PGPB factors such as able to dissolve phosphate, and produce HCN. The results obtained 251 isolates of endophytic bacteria. Based on the characterization results, the superior isolates capable of dissolving phosphate with an average diameter of 0.45 cm is isolate II.B.1D.3, and 11 isolates capable of producing high HCN. These isolates can be used as PGPB agents so that they can be useful in increasing plant growth and onion production and biocontrol in suppressing pathogens. Keywords: PGPB, endophyte, onion, phosphate, HCN

scholarly journals Allium cepa L. Inoculation with a Consortium of Plant Growth-Promoting Bacteria: Effects on Plant Growth and Development and Soil Fertility Status and Microbial Community

Proceedings ◽  
2021 ◽  
Vol 66 (1) ◽  
pp. 20
Author(s):  
Marika Pellegrini ◽  
Daniela M. Spera ◽  
Claudia Ercole ◽  
Maddalena del Gallo
Keyword(s):  
Microbial Community ◽  
Plant Growth ◽  
Soil Fertility ◽  
Allium Cepa ◽  
Bacterial Consortium ◽  
Plant Growth Promoting Bacteria ◽  
Allium Cepa L ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Molecular Profiles

The present work was aimed at investigating the effects of a four strains consortium—Azospirillum brasilense, Gluconacetobacter diazotrophicus, Herbaspirillum seropedicae, and Burkholderia ambifaria—on crops of Allium cepa L. and its soil health. The bacterial consortium was inoculated on seeds of two different onion varieties; inoculated seeds and control ones (treated with autoclaved inoculum) were sown in open-field and followed until harvest. Plant growth development parameters, as well as soil physico-chemical and molecular profiles (DNA extraction and 16S community sequencing on the Mi-Seq Illumina platform), were investigated. The results showed a positive influence of bacterial application on plant growth, with increased plant height (+18%), total chlorophylls (+42%), crop yields (+13%), and bulbs dry matter (+3%) than the control. The differences between control and treated experimental conditions were also underlined in the bulb extracts in terms of total phenolic contents (+25%) and antioxidant activities (+20%). Soil fertility and microbial community structure and diversity were also positively affected by the bacterial inoculum. At harvest, the soil with the presence of the bacterial consortium showed an increase of total organic carbon, organic matter, and available P and higher concentrations of nutrients than control. The ecological indexes calculated on the molecular profiles showed that community diversity was positively affected by the bacterial treatment. The present work allowed to remark the effective use of plant growth-promoting bacteria as a valid fertilization strategy to improve yield in productive landscapes, whilst safeguarding soil biodiversity.

scholarly journals Allium cepa L. Inoculation with a Consortium of Plant Growth-Promoting Bacteria: Effects on Plants, Soil, and the Autochthonous Microbial Community

2021 ◽  
Vol 9 (3) ◽  
pp. 639
Author(s):  
Marika Pellegrini ◽  
Daniela M. Spera ◽  
Claudia Ercole ◽  
Maddalena Del Gallo
Keyword(s):  
Microbial Community ◽  
Plant Growth ◽  
Allium Cepa ◽  
Bacterial Consortium ◽  
Total Phenolic ◽  
Plant Growth Promoting Bacteria ◽  
Allium Cepa L ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Molecular Profiles

The present work was aimed at investigating the effects of a four bacterial strain consortium—Azospirillum brasilense, Gluconacetobacter diazotrophicus, Herbaspirillum seropedicae, and Burkholderia ambifaria—on Allium cepa L. and on soil health. The bacterial consortium was inoculated on seeds of two different onion varieties; inoculated and Control seeds (treated with autoclaved inoculum) were sown in an open-field and followed until harvest. Plant growth development parameters, as well as soil physico–chemical and molecular profiles (DNA extraction and 16S community sequencing on the Mi-Seq Illumina platform), were investigated. The results showed a positive influence of bacterial application on plant growth, with increased plant height (+18%), total chlorophylls (+42%), crop yields (+13%), and bulb dry matter (+3%) with respect to the Control. The differences between Control and treatments were also underlined in the bulb extracts in terms of total phenolic contents (+25%) and antioxidant activities (+20%). Soil fertility and microbial community structure and diversity were also positively affected by the bacterial inoculum. At harvest, the soil with the presence of the bacterial consortium showed an increase in total organic carbon, organic matter, and available phosphorus, as well as higher concentrations of nutrients than the Control. The ecological indexes calculated from the molecular profiles showed that community diversity was positively affected by the bacterial treatment. The present work showed the effective use of plant growth-promoting bacteria as a valid fertilization strategy to improve yield in productive landscapes whilst safeguarding soil biodiversity.

scholarly journals Potensi Bakteri Endofit dari Tanaman Sagu (Metroxylon spp.) sebagai Agens Pemacu Pertumbuhan Tanaman Padi

2018 ◽  
Vol 14 (2) ◽  
pp. 75-80
Author(s):  
Yatni Yatni ◽  
Gratiana N C Tuhumury ◽  
Christoffol Leiwakabessy
Keyword(s):  
Plant Growth ◽  
Endophytic Bacteria ◽  
Growth Promoter ◽  
Plant Growth Promoting Bacteria ◽  
Test Results ◽  
Bacterial Isolates ◽  
The People ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Stems And Leaves

Sago is a staple plant of the people of Maluku and Papua, which have many benefits and advantages to continue to be developed. Endophytic bacteria are bacteria that live in plant tissues and colonize the intercellular and vascular systems. This study aims to obtain endophytic bacterial isolates from parts of the roots, stems, and leaves of sago plants which have the potential as agents for plant growth promoting bacteria. Endophytic bacteria are isolated from the roots, stems, and leaves. Based on the results of isolation, 21 isolates of endophytic bacteria were found. Then, the selection is done by hypersensitivity test and gram reaction test. The results of the selection obtained 20 isolates of endophytic bacteria that were not pathogenic. After that, it was followed by plant growth promoting test for endophytic bacterial isolates. The test results were obtained three isolates potentially as plant growth promoter that is STA1, STA6, and STA11. Keywords: endophytic bacteria, plant growth promoting, sago   ABSTRAK Tanaman sagu merupakan tanaman pokok masyarakat Maluku dan Papua, yang memiliki banyak manfaat dan keunggulan untuk terus dikembangkan. Bakteri endofit adalah bakteri yang hidup di dalam jaringan tanaman dan berkoloni pada daerah ruang interseluler dan sistem vascular. Penelitian ini bertujuan untuk mendapatkan isolat bakteri endofit dari bagian akar,batang dan daun tanaman sagu yang berpotensi sebagai agens pemacu pertumbuhan tanaman. Bakteri endofit diisolasi dari bagian akar, batang, dan daun. Berdasarkan hasil isolasi yang telah dilakukan didapati 21 isolat bakteri endofit. Kemudian seleksi dilakukan dengan uji hipersensitif dan uji reaksi gram. Hasil seleksi diperoleh 20 isolat bakteri endofit yang bukan patogen. Setelah itu dilanjutkan dengan uji pemacu pertumbuhan tanaman terhadap isolat bakteri endofit. Hasil pengujian tersebut diperoleh tiga isolat bakteri yang berpotensi sebagai pemacu pertumbuhan tanaman yaitu STA1, STA6, dan STA11. Kata kunci: bakteri endofit, pemacu pertumbuhan tanaman, sagu

scholarly journals Diversity and capacity to promote maize growth of bacteria isolated from the Amazon region

2016 ◽  
Vol 46 (2) ◽  
pp. 111-118 ◽  
Author(s):  
Krisle da SILVA ◽  
Liamara PERIN ◽  
Maria de Lourdes GOMES ◽  
Alexandre Cardoso BARAÚNA ◽  
Gilmara Maria Duarte PEREIRA ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Plant Growth ◽  
Endophytic Bacteria ◽  
Nifh Gene ◽  
Amazon Region ◽  
Forest Area ◽  
Plant Growth Promoting Bacteria ◽  
Plant Growth Promoting ◽  
Maize Plants ◽  
Growth Promoting

ABSTRACT Maize plants can establish beneficial associations with plant growth-promoting bacteria. However, few studies have been conducted on the characterization and inoculation of these bacteria in the Amazon region. This study aimed to characterize endophytic bacteria isolated from maize in the Amazon region and to assess their capacity to promote plant growth. Fifty-five bacterial isolates were obtained from maize grown in two types of ecosystems, i.e., a cerrado (savanna) and a forest area. The isolates were characterized by the presence of the nifH gene, their ability to synthesize indole-3-acetic acid (IAA) and solubilize calcium phosphate (CaHPO4), and 16S rRNA partial gene sequencing. Twenty-four bacteria contained the nifH gene, of which seven were isolated from maize plants cultivated in a cerrado area and seventeen from a forest area. Fourteen samples showed the capacity to synthesize IAA and only four solubilized calcium phosphate. The following genera were found among these isolates: Pseudomonas; Acinetobacter; Enterobacter; Pantoea; Burkholderia and Bacillus. In addition, eight isolates with plant growth-promoting capacity were selected for a glasshouse experiment involving the inoculation of two maize genotypes (a hybrid and a variety) grown in pots containing soil. Inoculation promoted the development of the maize plants but no significant interaction between maize cultivar and bacterial inoculation was found. A high diversity of endophytic bacteria is present in the Amazon region and these bacteria have potential to promote the development of maize plants.

scholarly journals Culturable endophytic bacteria from stems and leaves of garden pea (Pisum sativum L.)

2020 ◽  
Author(s):  
Ekaterina N. Vasileva ◽  
Gulnar A. Akhtemova ◽  
Alexey M. Afonin ◽  
Alexey Borisov ◽  
Igor A. Tikhonovich ◽  
...  
Keyword(s):  
Pisum Sativum ◽  
Plant Growth ◽  
Endophytic Bacteria ◽  
Taxonomic Position ◽  
Plant Growth Promoting Bacteria ◽  
Garden Pea ◽  
Pisum Sativum L ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Stems And Leaves

Background. Endophytic microorganisms inhabit internal tissues of most plants. However, little is known about endophytic community of the garden pea (Pisum sativum L.), an agriculturally important crop. Materials and methods. Culturable endophytic bacteria were isolated from sterilized stems and leaves of three pea genotypes: K-8274 (cv. Vendevil), K-3358 (unnamed cultivar), and cv.Triumph. The taxonomic position of isolates was determined by 16S rRNA gene sequencing. The plant growth-promoting capabilityof identified bacteria was tested on the roots of watercress (Lepidium sativum L.). Results. In total, out of 118 morphotypes of culturable endophytic bacteria identified, for 80 the taxonomic position was determined. Proteobacteria and Firmicuteswere dominant phyla, and Actinobacteria were present in minority. Eight bacterial isolates demonstrated the plant growth-promoting capability, and one of them KV17 (Rahnella sp.) maintained this capability after several passages and prolonged storage. Conclusion.The plant growth-promoting bacteria isolated from pea stems and leaves can become a component of microbiological preparations.

scholarly journals Lysinibacillus acetophenoni and Pseudomonas stutzeri with High Salt effect, Recovered from High Salinity Soil Area (Indo-Gangetic Plain of India)

2021 ◽  
pp. 17-26
Author(s):  
Parul Bhatt Kotiyal ◽  
Soni Singh ◽  
Sunita Rawat ◽  
Vikesh Vyas ◽  
Himani Negi
Keyword(s):  
Plant Growth ◽  
Saline Soil ◽  
Halophilic Bacteria ◽  
Economic Value ◽  
Soil Microbial Communities ◽  
Plant Growth Promoting Bacteria ◽  
Bacterial Strains ◽  
Gangetic Plain ◽  
Plant Growth Promoting ◽  
Growth Promoting

Soil salinity has affected many soil microbial communities as well as economic value of forest ecosystem for many years. The plant growth-promoting bacteria have developed several different mechanisms that have a positive influence on plant development and growth. Designated strain L-PB424 and P-PB466 was isolated and identified from saline soil of block Ashabutter khair forest in Punjab North zone in India, were investigated for their plant growth-promoting characters such as production of indole acetic acid, phosphate solubilization, Ammonium production and fermentation of polysaccharides. Comparative analysis of 16SrRNA gene sequences revealed that L-PB424 was closely related to Lysinibacillus manganicus DSM 26584 strain Mn1-7 (98.76%), on the other hand strain P-PB466 was closely related to Pseudomonas songnenensis strain NEAUST5-5. This research paper is a study in evaluation and variety of possible halophlic/halotolerant bacterial strains in salt-affected soils of block Ashabutter khair forest in Punjab North zone in India. The use of Halophilic bacteria in saline soil is interesting for future analysis and biotechnological development.

scholarly journals Screening and Assessment of Potential Plant Growth-promoting Bacteria Associated with Allium cepa Linn.

2020 ◽  
Vol 35 (2) ◽  
pp. n/a
Author(s):  
Brian Estuardo Samayoa ◽  
Fo-Ting Shen ◽  
Wei-An Lai ◽  
Wen-Ching Chen
Keyword(s):  
Plant Growth ◽  
Allium Cepa ◽  
Plant Growth Promoting Bacteria ◽  
Plant Growth Promoting ◽  
Growth Promoting
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