scholarly journals The correlation between ectoparasite infestation and total Vibrio parahaemolyticus bacteria in Pacific white shrimp (Litopenaeus vannamei) in Super Intensive Ponds

2021 ◽  
Vol 888 (1) ◽  
pp. 012003
Author(s):  
Amalisa ◽  
Gunanti Mahasri ◽  
Kismiyati
Keyword(s):  
Correlation Analysis ◽  
Vibrio Parahaemolyticus ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
Low Oxygen ◽  
Shrimp Culture ◽  
Shrimp Pond ◽  
Parasite Intensity ◽  
Heavy Infestation ◽  
Ectoparasite Infestation

Abstract This study aimed to determine the type, intensity, degree of ectoparasite infestation and its correlation to the total V. parahaemolyticus bacteria in super-intensive pacific white shrimp culture. In this study, sampling was carried out in 3 super-intensive pacific white shrimp pond areas spread out in East Java, namely Bangil, Tuban, and Lamongan with 50 shrimps (PL30-PL 40). The obtained data underwent regression and correlation analysis. Based on the results, there were three types of ectoparasites, namely Zoothamnium sp., Epistylis sp. and Vorticella sp. High ectoparasite intensity was found in pacific white shrimp from Lamongan and Tuban ponds, namely 76 and 55 individuals/shrimp, respectively, showing the heavy infestation. High total V. parahaemolyticus bacteria was found in Tuban (1.16 × 105 CFU/gr) and Lamongan (1.16 × 105 CFU / gr) ponds. Based on the results, the coefficient value was R = 0.807 showing positive correlation of V. parahaemolyticus with the increasing parasite intensity and low oxygen levels.

scholarly journals Genomic characterization of Vibrio parahaemolyticus from Pacific white shrimp and rearing water in Malaysia reveals novel sequence types and structural variation in genomic regions containing the Photorhabdus insect-related (Pir) toxin-like genes

2019 ◽  
Vol 366 (17) ◽  
Author(s):  
Chrystine Zou Yi Yan ◽  
Christopher M Austin ◽  
Qasim Ayub ◽  
Sadequr Rahman ◽  
Han Ming Gan
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Illumina Miseq ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
Phylogenomic Analysis ◽  
Genomic Characterization ◽  
Genomic Resource ◽  
Acute Hepatopancreatic Necrosis Disease ◽  
Genomic Regions

ABSTRACT The Malaysian and global shrimp aquaculture production has been significantly impacted by acute hepatopancreatic necrosis disease (AHPND) typically caused by Vibrio parahaemolyticus harboring the pVA plasmid containing the pirAVp and pirBVp genes, which code for Photorhabdus insect-related (Pir) toxin. The limited genomic resource for V. parahaemolyticus strains from Malaysian aquaculture farms precludes an in-depth understanding of their diversity and evolutionary relationships. In this study, we isolated shrimp-associated and environmental (rearing water) V. parahaemolyticus from three aquaculture farms located in Northern and Central Malaysia followed by whole-genome sequencing of 40 randomly selected isolates on the Illumina MiSeq. Phylogenomic analysis and multilocus sequence typing (MLST) reveal distinct lineages of V. parahaemolyticus that harbor the pirABVp genes. The recovery of pVA plasmid backbone devoid of pirAVp or pirABVp in some V. parahaemolyticus isolates suggests that the toxin genes are prone to deletion. The new insight gained from phylogenomic analysis of Asian V. parahaemolyticus, in addition to the observed genomic instability of pVa plasmid, will have implications for improvements in aquaculture practices to diagnose, treat or limit the impacts of this disease.

scholarly journals Complete Genome Sequence of a Deletion Mutant of Vibrio parahaemolyticus from Pacific White Shrimp (Penaeus vannamei)

2018 ◽  
Vol 6 (25) ◽  
Author(s):  
Siddhartha Kanrar ◽  
Arun K. Dhar
Keyword(s):  
Genome Sequence ◽  
Vibrio Parahaemolyticus ◽  
Deletion Mutant ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
Penaeus Vannamei ◽  
Content Type ◽  
A Genome ◽  
Acute Hepatopancreatic Necrosis Disease ◽  
Experimental Challenge

ABSTRACT Vibrio parahaemolyticus carrying the toxin genes pirA and pirB causes acute hepatopancreatic necrosis disease in shrimp. A genome sequence of V. parahaemolyticus strain R13 was determined that showed deletions of the entire pirA gene and the 5ʹ end of the pirB gene and does not cause the disease in experimental challenge.

scholarly journals Chitinilyticum aquatile gen. nov., sp. nov., a chitinolytic bacterium isolated from a freshwater pond used for Pacific white shrimp culture

2007 ◽  
Vol 57 (12) ◽  
pp. 2854-2860 ◽  
Author(s):  
Shu-Chen Chang ◽  
Wen-Ming Chen ◽  
Jih-Terng Wang ◽  
Ming-Chang Wu
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
Pacific White Shrimp ◽  
16S Rrna Gene Sequence ◽  
White Shrimp ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Shrimp Culture ◽  
Freshwater Pond

Strain c14T, originally isolated from surface water of a freshwater pond located in Pingtung (southern Taiwan) used for culture of Pacific white shrimp (Litopenaeus vannamei), was subjected to a polyphasic taxonomic approach. The strain exhibited strong chitinolytic activity and was able to grow under aerobic and anaerobic conditions by utilizing chitin exclusively as the carbon, nitrogen and energy source. Phylogenetic analysis of the 16S rRNA gene sequence revealed a clear affiliation of the proposed bacterium to the Betaproteobacteria, most closely related to Chitinibacter tainanensis S1T, Deefgea rivuli WB 3.4-79T and Silvimonas terrae KM-45T, with 94.6, 93.6 and 92.9 % 16S rRNA gene sequence similarity, respectively. The predominant fatty acids detected in cells of strain c14T were C16 : 0, C18 : 1 ω7c and summed feature 3 (C16 : 1 ω7c and/or C15 : 0 iso 2-OH). The G+C content of the genomic DNA was 69.5 (±1.0) mol%. Biochemical, physiological, chemotaxonomic and phylogenetic analyses showed that strain c14T could not be assigned to any known genus of the Betaproteobacteria. Therefore, strain c14T is classified within a novel genus and species, for which the name Chitinilyticum aquatile gen. nov., sp. nov. is proposed. The type strain of Chitinilyticum aquatile is c14T (=LMG 23346T =BCRC 17533T).

scholarly journals Cyanobacteria reduction in the integrated culture of Pacific white shrimp and West India pointed venus in a biofloc system

2021 ◽  
Vol 16 (1) ◽  
pp. 75-82
Author(s):  
P.C.M. Lima ◽  
◽  
M.M.S. Luna ◽  
C.V.F.S. Campos ◽  
E.P. Santos ◽  
...  
Keyword(s):  
Phytoplankton Community ◽  
West Indian ◽  
Quality Parameters ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
High Concentration ◽  
Shrimp Culture ◽  
The Pacific ◽  
Weight Yield ◽  
Growth Performances

This study aimed to evaluate the integrated culture of the Pacific white shrimp (Litopenaeus vannamei) and the West Indian pointed venus (Anomalocardia flexuosa) reared in a biofloc system. The performance of both species and the phytoplankton community were evaluated. Shrimp monoculture and three stocking densities of West Indian pointed venus (2.5, 5.0 and 7.5 Kg m-3) were evaluated in 0.05 m3 tanks. First of all, the addition of A. brasiliana did not affect the water quality parameters of the culture. Regarding the phytoplankton community, 13 taxa were found: 5 cyanobacteria, 4 dinoflagellates, 3 diatoms and 1 green algae. Although diatoms were dominant at the beginning of cultivation, in the middle and at the end a cyanobacterial dominance was observed (proven by ANOSIM and nMDS). The taxa that most contributed to the abundance of cyanobacteria were Aphanocapsa sp. and Pseudanabaena sp. Regarding the performance of the shrimp, all integrated treatments present the higher growth performances (final weight, yield and survival) when compared to shrimp monoculture. Moreover, gradual mortalities of A. brasiliana were observed, probably due to the high concentration of cyanobacteria. Thus, it can be observed that the integration of molluscs in shrimp culture can reduce the concentration of harmful algae and improve the zootechnical performance of the shrimp.

scholarly journals Analisis Kelayakan Usaha Budidaya Udang Vanname (Litopenaeus vannamei) di Keramba Jaring Apung Laut

2019 ◽  
Vol 13 (2) ◽  
pp. 175
Author(s):  
Pindo Witoko ◽  
Ninik Purbosari ◽  
Nuning Mahmudah Noor
Keyword(s):  
Net Present Value ◽  
Pacific White Shrimp ◽  
Shrimp Farming ◽  
White Shrimp ◽  
Cost Ratio ◽  
Net Benefit ◽  
Shrimp Culture ◽  
Benefit Cost Ratio ◽  
Net Cage ◽  
Benefit Cost

<p>Pacific white shrimp is one of the primadona of fishery commodities. The development of Pacific white shrimp can be done by using floating net cage (FNC). Shrimp culture in sea floating net cage is the one of alternative aquaculture of environmentally friendly shrimp cultivation and potentially become applicative technology of shrimp farming in the future. The aims of this study were to know the feasibility of white shrimp culture in sea floating net cage. The study was conducted by using descriptive and analytic method with 18 pieces of cage. Analysis of the feasibility using net present Value (NPV),Internal Rate of return (IRR), Net Benefit-Cost Ratio, Gross Benefit-Cost Ratio, Payback Period (PBP), and Break Even Point (BEP). The study result show that the NPV is IDR 43,315,360.00; IRR is 21.47%; net B/C ratio is 5.11, gross B/C ratio is 3.71; PBP is 6 months and 9 days and BEP is 1,837.82 kg of shrimp biomass or IDR 147,025,891.18 of the value of sales. The final result of feasibility analysis of shrimp culture in sea floating net cage is feasible to run.</p>

Estimating genetic parameters for resistance to Vibrio parahaemolyticus with molecular markers in Pacific white shrimp

Aquaculture ◽  
2020 ◽  
Vol 527 ◽  
pp. 735439
Author(s):  
Ding Lyu ◽  
Yang Yu ◽  
Qian Zhang ◽  
Zheng Luo ◽  
Quanchao Wang ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Vibrio Parahaemolyticus ◽  
Genetic Parameters ◽  
Pacific White Shrimp ◽  
White Shrimp

Effects of dietary shifts on the stable isotope signature of Pacific white shrimp Litopenaeus vannamei and implications for traceability

2020 ◽  
Vol 71 (10) ◽  
pp. 1294 ◽  
Author(s):  
Li Li ◽  
Natalee Kokkuar ◽  
Cui Han ◽  
Wenjing Ren ◽  
Shuanglin Dong
Keyword(s):  
Pacific White Shrimp ◽  
Food Sources ◽  
White Shrimp ◽  
Isotopic Equilibrium ◽  
Shrimp Culture ◽  
Δ13c And Δ15n ◽  
Dietary Changes ◽  
Commercial Feed ◽  
Seafood Products ◽  
Dietary Shifts

Because the number of mislabelled seafood products in the globalised market is increasing, there is a need for additional effective technologies to trace the origin of these products. Several issues need to be clarified regarding the δ13C and δ15N analyses used to trace seafood products in order to increase their reliability. Herein, a laboratory experiment involving dietary shifts was conducted with shrimp culture to demonstrate that dietary changes elicit isotope changes. Feeding shrimp four different food sources with different isotope values revealed a significant relationship for δ13C and δ15N values between shrimp and food sources (P&lt;0.05). The shrimp fed different brands of commercial feed had significantly different δ15N values (P&lt;0.05), but not the δ13C values (P&gt;0.05) at the end of the study. However, the δ13C and δ15N values for shrimp that were fed live food were much higher than those for shrimp fed on commercial feed (P&lt;0.05), indicating that it is more reliable to distinguish seafood products using a method with distinctively different food sources. The shrimp rapidly achieve isotopic equilibrium with their diets after 3 weeks, and, for traceability, it is recommended to sample shrimp being consistently fed with one feed for more than 3 weeks.

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