scholarly journals Kappa casein (CSN3) gene polymorphism and its effect on cumulative milk yields of Holstein Friesian dairy cattle

2021 ◽  
Vol 902 (1) ◽  
pp. 012047
Author(s):  
S A Asmarasari ◽  
C Sumantri ◽  
A Gunawan ◽  
E Taufik ◽  
A Anggraeni ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Milk Proteins ◽  
Statistical Association ◽  
Rt Pcr ◽  
Casein Gene ◽  
Lactation Length ◽  
Holstein Friesian ◽  
Pcr Method ◽  
Polymerase Chain ◽  
Crucial Part

Abstract Kappa casein (CSN3) is a standout amongst the most vital milk proteins in mammals that assumes a crucial part in milk quality and coagulation. This study aimed to determine genetic polymorphism of the Kappa casein gene (CSN3) and associate its genotype variants on various cumulative milk yields in Holstein Friesian (HF) dairy cattle. A number of 61 blood samples were collected from 2 Holstein Friesian populations, respectively, from IRIAP Breeding Station in Ciawi (61) and Lembang Artificial Insemination Center (Lembang AIC) (17). Real Time -Polymerase Chain Reaction (RT-PCR) method was used to identify variant genotypes of the Kappa Casein gene. In population were detected all three genotypes GG, GT, and TT. The most frequent genotype was TT, with a frequency of 0.63. Results from the statistical association analysis between g.13975G>T CSN3 genotype and cumulative milk yield in standard lactation length were not significant.

scholarly journals Development of RT-PCR Based Diagnosis of SARS-CoV-2

2021 ◽  
Author(s):  
Rutuja Sunil Patankar ◽  
Vasudeo Pandharinath Zambare
Keyword(s):  
World Wide ◽  
Method Development ◽  
Vaccine Development ◽  
Whole Genome Sequence ◽  
Molecular Technique ◽  
Rt Pcr ◽  
Global Priority ◽  
Diagnosis Method ◽  
Pcr Method ◽  
Polymerase Chain

In the 2020, COVID-19 pandemic disease created an havoc situation world widely and mainly caused by Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2). It has been challenging task for researchers, scientists and medico-pharmaceutical organisations to find out rapid and reliable diagnosis methods. Among the all testing services, a Reverse Transcription Polymerase Chain Reaction (RT-PCR) is the more accurate, rapid and authenticated molecular technique used for most of the diagnosis of major diseases. It has been a global priority to fix the rapid diagnosis method to combat against the pandemic COVID-19. Thus, the present chapter mainly focussing on the progress of RT-PCR method development though various processes of data collection on isolation of whole genome sequence, its primer and method designing. In this scenario, India suddenly become the global leader for vaccine development and hence the challenges and RT-PCR kit development in India and rest of the world has been be discussed. World wide many Government and private agencies and industries have taken an initiative for diagnosis of SARS-CoV-2 hence this chapter also summarised the scope of RT-PCR to combat pandemic situation in future.

scholarly journals Deteksi Plasmodium falciparum dengan menggunakan metode real-time polymerase chain reaction di daerah Likupang dan Bitung

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
David C. Tooy ◽  
Janno B. Bernadus ◽  
Angle Sorisi
Keyword(s):  
Polymerase Chain Reaction ◽  
Plasmodium Falciparum ◽  
Real Time ◽  
Real Time Pcr ◽  
18S Rrna ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Negative Results ◽  
Pcr Method ◽  
Polymerase Chain

Abstract: Malaria is one of the most important parasitic disease which is caused by Plasmodium spp. There are approximately 1,2 billion people in the world with high risk of getting malaria. Plasmodium falciparum (P. falciparum) is the cause of tropical malaria or falciparum malaria, and is responsible for most of the mortality rate. Currently, real-time polymerase chain reaction (RT-PCR) is being studied as an alterative of conventional malarian examination. Mangold et al reported that RT-PCR have 94.1% sensitivity and 100% specificity compared to microscopic examination in detecting P. falciparum. The aim of this research is to detect the presence of P. falciparum using RT-PCR in Likupang and Bitung region. This research were using descriptive design to find out the capability of real-time PCR method to detect P. falciparum in Likupang dan Bitung region. The researcher have examined 71 samples which are fulfill the research sample’s criteria. Postive results of P. falciparum found in 18 samples (25,3%) and negative results in 53 samples (74,6%) of total 71 samples with using RT-PCR. No positive results were found in samples from Likupang. There are positive result of P. falciparum in samples from Bitung. It is concluded that RT-PCR method can detect the presence of P. falciparum from the samples obtained from Likupang and Bitung based on the presence of its DNA. This detection efford is done by using 18S rRNA as target gene and ajust specific temperature on the RT-PCR instrument.Keywords: Plasmodium falciparum, Real-time Polymerase Chain Reaction (PCR), DetectionAbstrak: Malaria merupakan salah satu penyakit penting yang disebabkan oleh parasit Plasmodium spp. Kira-kira 1,2 miliar penduduk dunia memiliki risiko tinggi untuk mendapat malaria. Di Indonesia sendiri, terdapat 343.527 kasus terkonfirmasi dan 45 kematian karena malaria. Plasmodium falciparum (P. Falciparum) merupakan penyebab dari malaria tropika atau malaria falsiparum, dan bertanggung jawab atas sebagian besar angka mortalitas. Saat ini Real-Time Polymerase Chain Reaction (RT-PCR) telah banyak diteliti sebagai alternatif dari pemeriksaan malaria. Mangold dkk melaporkan bahwa real-time PCR memiliki nilai sensitivitas 94,1% dan nilai spesifisitas 100% terhadap pemeriksaan mikroskopis dalam mendeteksi P. falciparum. Penelitian bertujuan untuk mendeteksi P. falciparum dengan menggunakan RT-PCR di daerah Likupang dan Bitung. Penelitian ini menggunakan rancangan penelitian deskriptif untuk mengetahui kemampuan metode real-time PCR dalam mendeteksi P. falciparum di daerah Likupang dan Bitung. Tujuan penelitian ini ialah untuk mendeteksi keberadaan P. falciparum dengan menggunakan metode real-time PCR di daerah Likupang dan Bitung. Peneliti memeriksa 71 sampel darah yang memenuhi kriteria sampel penelitian. Hasil positif P. falciparum ditemukan pada 18 sampel (25,3 %) dan hasil negatif pada 53 sampel (74,6 %) dari total 71 sampel dengan menggunakan RT-PCR. Tidak ditemukannya hasil positif P. falciparum pada sampel dari Likupang. Ditemukan hasil positif P. falciparum pada sampel dari Bitung. Simpulan: Metode RT-PCR dapat mendeteksi P. falciparum berdasarkan keberadaan DNA-nya pada sampel yang diperoleh dari daerah Likupang dan Bitung. Deteksi ini berhasil dilakukan dengan menggunakan 18S rRNA sebagai gen target dan pengaturan suhu tertentu pada instrument RT-PCR.Kata kunci: P. falciparum, Real-time Polymerase Chain Reaction (PCR), Detection

scholarly journals The PI3KCA and AKT Inhibitory Activities of Litsea Cubeba Lour. Fruits and Heartwoods Towards Hela Cells

2019 ◽  
Vol 7 (9) ◽  
pp. 1422-1424
Author(s):  
Aminah Dalimunthe ◽  
Poppy Anjelisa Zaitun Hasibuan ◽  
Denny Satria
Keyword(s):  
Gene Expression ◽  
Cervical Cancer ◽  
Cell Culture ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Litsea Cubeba ◽  
Pcr Method ◽  
Polymerase Chain ◽  
Inhibitory Activities

AIM: To investigated the activities of chloroform fractions at pH 7 of Litsea cubeba Lour. Fruits and heartwoods (CF-7F and CF-7H) in decrease expression of PI3KCA, Akt-1 and Akt-2 genes towards cervical cancer cell culture (HeLa) experiments in vitro. MATERIAL AND METHODS: CF-7F and CF-7H (12.5 and 25 µg/mL) were tested for its potential inhibition on gene expression of PI3KCA, Akt-1 and Akt-2 genes by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) method. RESULT: CF-7F and CF-7H were showed the activity to reduce the expression of PI3KCA, Akt-1 and Akt-2 genes. CONCLUSION: Our results suggest that CF-7F and CF-7H significantly inhibit the expression of PI3KCA, Akt-1 and Akt-2 genes.

scholarly journals Genetic polymorphism of kappa-casein gene in Friesian Holstein: a basic selection of dairy cattle superiority

2017 ◽  
Vol 42 (4) ◽  
pp. 213 ◽  
Author(s):  
S. D. Volkandari ◽  
I. Indriawati ◽  
E. T. Margawati
Keyword(s):  
Dairy Cattle ◽  
Milk Protein ◽  
Casein Gene ◽  
Genotype Frequencies ◽  
Pcr Rflp ◽  
Milk Component ◽  
Milk Performance ◽  
Pcr Method ◽  
Two Populations ◽  
Selection Of

Caseins are milk protein subdivided into four main groups which are αS1, αS2, β-casein and kappa-casein (CSN3). Kappa-casein gene influences the manufacturing of milk properties. The aim of this study was to identify the kappa-casein gene polymorphism in Friesian Holstein (FH) cattle. Fifty nine (59) samples consisted of 32 (Malang), 10 (Sukahati Bogor) and 17 (Research Center for Biotechnology-Indonesian Institute of Sciences’s collections)were applied in this study. DNA samples were extracted by high concentrated NaCl and quantified by spectrophotometer. The kappa-casein gene was amplified at 379 bp fragment by PCR method using a pair primer of kappa-casein at 56oC annealing for 30 cycles. PCR-RFLP technique with HindIII was used for genotyping analysis. The result showed that there were three variants of genotypes (AA, AB and BB) in two populations from Malang and RC for Biotechnology-LIPI’s collection while cattle from Sukahati had only AA and AB genotypes. The averages of genotype frequencies were 65.28%; 65.28%; and 3.00% for AA, AB and BB genotypes respectively while frequencies of 0.81 and 0.19 were for A and B alleles, respectively. FH cattle populations were in equilibrium genetics. This finding concludes that polymorphism was found in three of FH populations with A allele was more common in kappa-casein locus. B allele is known having association with milk production, milk component and cheese yield. Increasing of B allele would influence on milk performance of FH cattle. Explorations of quantitative, qualitative and molecular genetics are important to improve dairy cattle performance.

Expression of IGF2 and IGF receptor mRNA in bovine nuclear transferred embryos

Zygote ◽  
2003 ◽  
Vol 11 (3) ◽  
pp. 245-252 ◽  
Author(s):  
Dong-Wook Han ◽  
Sang-Jin Song ◽  
Sang Jun Uhum ◽  
Jeong-Tae Do ◽  
Nam-Hyung Kim ◽  
...  
Keyword(s):  
Nuclear Transfer ◽  
Donor Cell ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Pcr Method ◽  
Igf Receptor ◽  
Polymerase Chain ◽  
Low Efficiency ◽  
Insight Into

Incomplete reprogramming of the donor cell nucleus after nuclear transfer (NT) probably leads to the abnormal expression of developmentally important genes. This may be responsible for the low efficiency of cloned animal production. Insulin-like growth factor 2 (IGF2) and IGF2 receptor (IGF2R) are imprinted genes that play important roles in preimplantation development. To obtain an insight into abnormal gene expression after nuclear transfer, we assessed the transcription patterns of IGF2-IGF2R in single in vitro fertilised and cloned embryos by reverse-transcription polymerase chain reaction (RT-PCR). IGF2R expression did not differ significantly but IGF2 was more highly expressed in cloned embryos than in IVF embryos (p < 0.05). This was confirmed by a quantitative RT-PCR method. Thus, incomplete reprogramming may induce abnormal transcription of IGF2 in cloned embryos.

Detection of Brachyspina carriers within Polish Holstein-Friesian bulls

2015 ◽  
Vol 18 (2) ◽  
pp. 453-454
Author(s):  
A. Ruść ◽  
S. Kamiński
Keyword(s):  
Dairy Cattle ◽  
Genetic Defect ◽  
Recessive Mutation ◽  
Cattle Population ◽  
Systematic Screening ◽  
Holstein Friesian ◽  
The Us ◽  
Pcr Method ◽  
Friesian Cattle

Abstract The aim of this paper was to verify the hypothesis whether carriers of genetic defect Brachyspina occur in the Polish Holstein-Friesian Cattle. PCR method was used to screen 78 Polish Holstein-Friesian bulls. Eight bulls were identified as heterozygotes for 3,3 kb deletion in the FANCI gene – the mutation causing Brachyspina defect. All carriers were sons of 3 sires: Cleitus Jabot, Sandy-Valley Bolton ET and Coyne-Farms Dorcy ET which were descendants of the US sire Sweet Haven Tradition (HOUSAM 1682485). Systematic screening of young bulls having in the pedigree Barchyspina carrier is necessary to prevent spreading of the recessive mutation in the dairy cattle population in Poland.

scholarly journals Detection of Flavescence Dorée Phytoplasma in Grapevine by Reverse-Transcription PCR

Plant Disease ◽  
2007 ◽  
Vol 91 (11) ◽  
pp. 1496-1501 ◽  
Author(s):  
P. Margaria ◽  
C. Rosa ◽  
C. Marzachì ◽  
M. Turina ◽  
S. Palmano
Keyword(s):  
Reverse Transcription ◽  
Large Scale ◽  
Diagnostic Methods ◽  
Kappa Index ◽  
Rt Pcr ◽  
Flavescence Dorée ◽  
Reverse Transcription Pcr ◽  
Novel Method ◽  
Pcr Method ◽  
Polymerase Chain

Flavescence dorée (FD) is the most serious phytoplasma disease of grapevine. This report describes a novel method of detecting FD phytoplasma based on reverse-transcription polymerase chain reaction (RT-PCR) on 16S ribosomal RNA (16SrRNA) which will greatly improve mass screening of infected grapevines. A rapid protocol for extracting sap from whole leaves or midveins and successive one-tube amplification by RT-PCR was applied to grapevine samples with or without symptoms collected from different areas of Piedmont (northwestern Italy). Results were compared with those obtained using one of the current diagnostic methods that utilizes nested PCR on phytoplasma DNA-enriched preparations. A Cohen's kappa index of 0.76 indicated a substantial agreement between the two sets of results. The RT-PCR method has the advantage of being a rapid, reliable, and sensitive assay for large-scale screening of grapevines.

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