scholarly journals Callus inducement of Toona sinensis: Potential agents against SARS-Corona virus replication

2021 ◽  
Vol 914 (1) ◽  
pp. 012078
Author(s):  
A I Putri ◽  
T Herawan ◽  
Prastyono ◽  
L Haryjanto ◽  
M A Fauzi ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Virus Replication ◽  
Callus Induction ◽  
Callus Formation ◽  
Ms Medium ◽  
Cell Weight ◽  
Toona Sinensis ◽  
Corona Virus ◽  
Dry Cell Weight

Abstract Bioprospecting of anti-SARS-corona virus phytochemical agents is an important issue today as an alternative to chemical drugs whose effectiveness has not been fully successful with no light effect. Quercetin, a component of Toona sinensis young leaves extract, was reported to have anti-viral activity against SARS-corona virus replication in vitro. Currently, the low efficiency of secondary metabolite production is an obstacle. Production of secondary metabolites from callus induction in vitro is considered more efficient and in a short time for commercial applications. The composition of hormones in callus media affects the secondary metabolites formed. The study was conducted to determine the effect of synthetic hormones auxin (BAP) and cytokinin (NAA) on callus percentage, fresh and dry callus cell weight and the average time of callus formation. The call percentage (100%) was obtained in different combinations of BAP and NAA in MS medium. The highest fresh weight of callus (165.50 gL−1) and dry cell weight (28.47 gL−1) were observed in MS medium (1.5 mgL−1 BAP and 1 mgL−1 NAA) within 7 days initial formation time of callus. The results showed that all the indices measured were positively correlated with callus induction in T. sinensis.

scholarly journals Callus Induction in Baru (Dipteryx alata Vog.) Explants

2018 ◽  
Vol 47 (2) ◽  
pp. 538-543
Author(s):  
Rodrigo Kelson S. REZENDE ◽  
Ana Maria N. SCOTON ◽  
Maílson V. JESUS ◽  
Zeva V. PEREIRA ◽  
Fernanda PINTO
Keyword(s):  
Ascorbic Acid ◽  
Callus Induction ◽  
Callus Formation ◽  
Leaf Explants ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Alternative Technique ◽  
Propagation Methods ◽  
Dipteryx Alata

Baru (Dipteryx alata Vog.) is a species with great economic and environmental potential; it has popular acceptance, besides being a very productive species. Alternative propagation methods are important for species maintenance and exploration. Thus, micropropagation emerged as an alternative technique, providing genetic stability and the production of a large number of seedlings. The aim of the present investigation was to develop a callus induction protocol for in vitro baru explants. The tested explants were nodal, internodal and foliar segments. The explants were disinfected for 30 seconds in 70% alcohol (v/v) and 2 minutes in sodium hypochlorite (1.25% active chlorine). This was followed by triple washing. The inoculation was carried out in test tubes containing 15 mL MS medium (30 g L-1 sucrose, 6 g L-1 agar and 100 mg L-1 ascorbic acid) supplemented with 2.0 mg L-1 naphthalene acetic acid (NAA). The solution also contained 0.0, 2.5 or 5.0 mg L-1 of 6-benzylaminopurine (BAP) with the pH adjusted to 5.8. In the incubation phase, the explants were cultured for seven days in the dark and then subjected to a photoperiod of 16 hours (43 µmol m-2 s-1) at 25 ± 2 °C. The treatments were studied with 2.5, 5.0, 7.5 or 10.0 mg L-1 BAP additions to the MS. Callus formation, contamination and oxidation evaluations were undertaken. The results obtained when using 2.0 mg L-1 NAA concluded that such a treatment should be used to induce callogenesis from nodal explants, while for the tested baru leaf explants, the best results for callus formation were given by the combination of 2.0 mg L-1 NAA with 2.5 mg L-1 of BAP to.

scholarly journals In Vitro Regeneration Of Brinjal (Solanum Melongena L.)

1970 ◽  
Vol 36 (3) ◽  
pp. 397-406 ◽  
Author(s):  
BP Ray ◽  
L Hassan ◽  
KM Nasiruddin
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
Callus Formation ◽  
Solanum Melongena ◽  
Ms Medium ◽  
Fresh Weight ◽  
Normal Environment ◽  
Regenerated Plantlets

The effect of different explants and concentrations of BAP and NAA on induction of callus and plant regeneration of brinjal cv. Jhumki were investigated. The treatment combinations were BAP (0. 2.0. 3.0, and 4.0 mg/l) and NAA (0. 0.1, 0.5, and 1.0 mg/l). The rate of callus formation varied in different treatments. The highest amount of callus (48.66%) was produced on MS medium containing 2.0 mg/l BAP and 0.5 mg/l NAA from stem, and 8.2 days required for callus induction. The highest fresh weight of callus was 1.12g from stem and 0.48g from root. The number of shoot regenerated through callus from stem containing 2.0 mg/l BAP and 0.5 mg/l NAA was 3.4 (23.287%) and days required for 38.8 days. All regenerated plantlets survived in normal environment. Keywords: NAA; BAP; regeneration; brinjal. DOI: http://dx.doi.org/10.3329/bjar.v36i3.9268 BJAR 2011; 36(3): 397-406

scholarly journals In Vitro Plant Regeneration from Cultured Cotyledons of Cotton (Gossypium herbaceum L.)

1970 ◽  
Vol 46 (3) ◽  
pp. 359-364
Author(s):  
AN Chowdhury ◽  
MZ Rahman ◽  
A Samad ◽  
AKMS Alam ◽  
S Khaleda
Keyword(s):  
Plant Regeneration ◽  
Root Development ◽  
Callus Induction ◽  
Callus Formation ◽  
Ms Medium ◽  
Natural Conditions ◽  
Callus Proliferation ◽  
Gossypium Herbaceum ◽  
Number Of Shoots

The effect of cytokinins on callus proliferation from cotyledons and plantlet development was studied in cotton. The frequency of callus induction was observed on MS medium enriched with a variety of cytokinins in different concentrations. With the increase of cytokinin concentration, the percentage of callus formation, percentage of shoot developing calli and number of shoots/calli were increased. Among the three different cytokinins studied, BA showed the highest performance. The highest percentage of callus (6.55%) and shoot developing calli (5.87%) was obtained on MS with 1.0 mg/l BA. Highest number of shoots (3.02) per calli was observed on MS media supplemented with 1.0 mg/l Kn. The rooting media composed of MS medium, 0.6% agar, sucrose and fortified with 2.0 mg/l NAA induced root development at the highest percentage (41.23%) with maximum number of roots (3.61) per cutting and length of root (3.62 cm) per culture. The plantlets were acclimatized in natural conditions. Key words: In vitro; Callus; Cotyledons; Cytokinin; Plantlet; Acclimatization DOI: http://dx.doi.org/10.3329/bjsir.v46i3.9043 BJSIR 2011; 46(3): 359-364

scholarly journals In vitro regeneration protocol development via callus formation from stem explant of tomato

2016 ◽  
Vol 1 (3) ◽  
pp. 589-599
Author(s):  
Meherunnesa Papry ◽  
SM Ahsan ◽  
Sayeed Shahriyar
Keyword(s):  
Callus Induction ◽  
Callus Formation ◽  
In Vitro Regeneration ◽  
Germination Rate ◽  
Plant Biotechnology ◽  
Stem Explants ◽  
Ms Medium ◽  
Good Efficiency ◽  
Shoot Initiation

The experiment was conducted on in vitro regeneration of tomato at the Plant Biotechnology Laboratory, Department of Horticulture, Patuakhali Science and Technology University, Patuakhali. The objective was to develop an efficient regeneration protocol in tomato through callus induction for subsequent plantlet regeneration. Seeds were inoculated on MS medium where germination rate was 78.4%. The stems of in vitrocultured seedlings were used as explants. Different concentrations and combinations of growth regulators were added to MS medium to observe their efficacy on callus induction, shoot initiation and root formation. Stem explants cultured on MS medium fortified with 2 mg/L BAPgave the highest number of shoots (3.0) at 45 DAC. Among the concentrations of PGRs, 0.25 mg/L IAA produced the highest length (4.064 cm) of plantlets, number (5.0) of leaves and fresh weight (0.663 g) of plantlets with the stem explants at 45 DAC. The concentration of 0.5 mg/L IAA produced the highest number (21.00) of roots/plantlet, length (7.676 cm) of roots at 45 DAC, from the same explants. The highest survival rate of in vitro regenerated plantlets in the pot was 70.00 % with the stem explants. The results of the current study showed significant increase in the growth of callus of Solanumlycopersicon Mill. Indicating a good efficiency of the optimized media composition and the experimental model used in comparison to other studies of similar nature.Asian J. Med. Biol. Res. December 2015, 1(3): 589-599

scholarly journals INDUKSI KALUS CENGKEH DARI EKSPAN DAUN MENGGUNAKAN 2,4-D SECARA IN VITRO

2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Yulianti Rasud ◽  
Zainuddin Basri ◽  
Nirwan Sahiri
Keyword(s):  
Growth Regulators ◽  
Callus Induction ◽  
Callus Formation ◽  
Cell Mass ◽  
Medium Composition ◽  
Ms Medium ◽  
Plant Parts ◽  
Randomized Design ◽  
Completely Randomized Design

ABSTRACT Callus induction is one method of tissue culture which is done by stimulating cell division continuously from certain plant parts such as leaves, roots, stems, and so on by using growth regulators to form cell mass. The cell mass (callus) will then regenerate through organogenesis or embryogenesis to become a new plant. One of the growth regulators used for callus induction is 2,4-D. The aims of this experiments was to evaluate the best concentration of 2,4-D for callus induction of clove leaves. The experiment used Completely Randomized Design with treatment tested was concentrations of 2,4-D, consisted of six levels, namely 0.5 ppm, 1.0 ppm, 1.5 ppm, 2.0 ppm, 2.5 ppm and 3.0 ppm. Results of this experiments indicated that the best medium composition for callus induction was MS medium supplemented with 0.5 ppm 2,4-D.  In the medium composition, the fastest callus formation, namely 6.00 weeks after culture and the percentage of callus formation reached 100% with the color and texture of the resulting callus white and crumb. Keyword : Callus Induction, Clove, 2,4-DABSTRAK Induksi kalus merupakan salah satu metode kultur jaringan yang dilakukan dengan jalan memacu pembelahan sel secara terus menerus dari bagian tanaman tertentu seperti daun, akar, batang, dan sebagainya dengan menggunakan zat pengatur tumbuh hingga terbentuk massa sel. Massa sel (kalus) tersebut selanjutnya akan beregenerasi melalui organogenesis ataupun embriogenesis hingga menjadi tanaman baru. Salah satu zat pengatur tumbuh yang digunakan untuk induksi kalus adalah 2,4-D. Penelitian ini bertujuan menentukan konsentrasi 2,4-D yang lebih baik untuk induksi kalus daun cengkeh.  Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan tiga kali ulangan. Media dasar yang digunakan adalah media MS yang ditambahkan berbagai konsentrasi 2,4-D yaitu 0,50 ppm, 1,5 ppm, 2 ppm, 2,5 ppm, dan 3 ppm. Hasil penelitian menunjukkan bahwa komposisi media yang terbaik untuk induksi kalus daun cengkeh adalah media MS yang ditambahkan 0,5 ppm 2,4-D.  Pada komposisi media tersebut diperoleh saat muncul kalus paling cepat, yaitu rata-rata 6,00 MST dengan persentase pembentukan kalus tertinggi mencapai 100% dengan warna dan tekstur kalus yang dihasilkan putih dan remah. Kata Kunci :  Induksi Kalus, Cengkeh, 2,4-D.

scholarly journals In vitro cultivation and regeneration of Solanum melongena (L.) using stem, root and leaf explants

1970 ◽  
Vol 1 (1) ◽  
pp. 49-54 ◽  
Author(s):  
Bishnu Pada Ray ◽  
Lutful Hassan ◽  
Smreeti Kana Sarker
Keyword(s):  
Key Words ◽  
Callus Induction ◽  
Callus Formation ◽  
Solanum Melongena ◽  
Leaf Explants ◽  
In Vitro Cultivation ◽  
Ms Medium

The treatment combinations was BAP (0, 2.0, 3.0 and 4.0 mg/L) and NAA (0, 0.1, 0.5, and 1.0 mg/L). The rate of callus formation varied in different treatments. The highest amount of callus (48.66%) was produced on MS medium containing 2.0 mg/l BAP and 0.5 mg/l NAA from stem and 8.2 days required for callus induction. The number of shoot regenerated through callus from stem containing 2.0 mg/l BAP and 0.5 mg/l NAA was 3.4 (23.287%) and days required for 38.8 days. Key words: Regeneration; BAP; NAA. Nepal Journal of Biotechnology. Jan. 2011, Vol. 1, No. 1 : 49-54

scholarly journals In Vitro plant regeneration from leaf explants of Tagetes erecta L.

2021 ◽  
Vol 56 (2) ◽  
pp. 69-74
Author(s):  
JL Munshi ◽  
R Baksha ◽  
MZ Rahaman ◽  
NN Huque ◽  
EA Zinat ◽  
...  
Keyword(s):  
Callus Induction ◽  
Callus Formation ◽  
Leaf Shape ◽  
Leaf Explants ◽  
Multiple Shoots ◽  
Tagetes Erecta ◽  
Ms Medium ◽  
Shoot Bud ◽  
Tagetes Erecta L

Regeneration of multiple shoots via callus induction and organogenesis was obtained from young leaf explants of the field grown marigold (Tagetes erecta L.). Callus induction and shoot regeneration at various frequencies were observed using different concentrations and combinations of growth regulators. Highest percentage (90%) of callus formation was observed within two weeks on MS medium supplemented with 5.0 mg/l BAP with 2.5 mg/l NAA. The maximum percentage (80%) of shoot bud formation (10±0.5/callus) was obtained from MS medium containing 1.0 mg/l BAP with 0.5 mg/l kinetin. The regenerated shoots developed highest percentages (90%) of roots on half strength MS medium supplemented with 1.0 mg/l IBA. The plantlets when transferred into potsoil 80% survived. Regenerated plants were morphologically uniform with normal leaf shape and growth pattern. Bangladesh J. Sci. Ind. Res.56(2), 69-74, 2021

scholarly journals Induction of Morphogenic Callus and In Vitro Regeneration of Sugarcane (Saccharum Officinarum L.)

2021 ◽  
Vol 5 (1) ◽  
pp. 61-67
Author(s):  
Sitti Inderiati ◽  
FNU Yanti ◽  
Eka Ria Mentari
Keyword(s):  
Callus Induction ◽  
Callus Formation ◽  
In Vitro Regeneration ◽  
Shoot Proliferation ◽  
Saccharum Officinarum ◽  
Induction Medium ◽  
Ms Medium ◽  
Shoot Initiation ◽  
Morphogenic Callus

In vitro propagation is a method to produce massive healthy new planting materials quickly. An experiment was carried out for morphogenic callus induction and regeneration of a domestic sugarcane variety. The Explants used was an inert folded leaf and incubated on modified MS medium augmented with 1 mg/l, 2.5 mg/l, and 5 mg/l of 2,4-D for callus induction. The leaf calluses were subcultured on MS medium enriched with different growth regulators for shoot initiation and multiplication. The highest percentage of callus formation was achieved in the medium containing 2.5 mg/l of 2,4-D, while the fastest callus initiation was noticed in MS medium supplemented with 5 mg/l 2,4-D, and maximum proliferation and the morphogenic response of callus were obtained in 3rd subculture. Two types of callus observed on the induction medium were dry nodular friable and smooth compact. This highly morphogenic callus was white to white creamy in color and easy to separate.   The highest shoot proliferation rate was found on the medium containing 2 mg/l Kinetin + 1 mg/l IAA and no growth was noticed on the medium containing Kinetin alone. Therefore, the study suggests that the growth hormone of cytokinin in combination with auxin is necessary for in vitro regeneration of sugarcane callus culture.

scholarly journals In vitro regeneration of popular tobacco varieties of Bangladesh from leaf disc

1970 ◽  
Vol 35 (1) ◽  
pp. 125-134 ◽  
Author(s):  
MA Rahman ◽  
MA Alam ◽  
MR Hossain ◽  
A Hossain ◽  
R Afroz
Keyword(s):  
Callus Induction ◽  
Callus Formation ◽  
In Vitro Regeneration ◽  
Leaf Disc ◽  
Shoot Formation ◽  
Plantlet Regeneration ◽  
Regeneration Ability ◽  
Ms Medium ◽  
Leaf Discs

Regeneration ability of five Nicotiana varieties viz., Virginia, Jati, Motihari, CC Bengal and Sumatra were investigated via callus induction using leaf discs. Explants were cultured on MS medium supplemented with different concentrations and combinations of plant growth regulators. Callus formation frequency was 67.20%. Among the varieties used, Motihari induced the highest percentage (97.50%) of callus followed by Jati (92.50%) in 2.0 rng/L Kinetin and 2.0 mg/L IAA. Shoots were induced from calli cultured on the same medium. Maximum shoot formation from leaf discs was 82.50% on medium supplemented with 2.0 mg/L Kinetin and 2.0 mg/L IAA. It was also revealed from this study that Motihari was the best variety for callus formation and subsequent plantlet regeneration which is a pre-requisite for vector mediated transformation for varietal improvement of Nicotiana species. The rooting response of regenerated shoots was observed by using ½ MS medium with IBA (0.0, 0.5, and 1.0 mg/L). The highest root formation was found in Motihari (90%) with ½ MS medium supplemented with 0.5 mg/L IBA. After that regenerated plantlets with plenty of roots were transferred successfully to pots and subsequently to the field. Keywords: Tobacco; Nicotiana; in vitro regeneration; callus induction; plantlet regeneration; leaf disc; phytohormone. DOI: 10.3329/bjar.v35i1.5873Bangladesh J. Agril. Res. 35(1) : 125-134, March 2010

Callus Induction and Plant Regeneration in Rauvolfia Serpentina (L.) Benth Ex. Kurz.

2009 ◽  
Vol 24 ◽  
pp. 82-88 ◽  
Author(s):  
Saraswoti Aryal ◽  
Sanu Devi Joshi
Keyword(s):  
Acetic Acid ◽  
Callus Induction ◽  
Coconut Milk ◽  
Ms Medium ◽  
Rauvolfia Serpentina ◽  
History Museum ◽  
Short Period ◽  
Murashige Skoog ◽  
Callus Tissue Culture

Rauvolfia serpentina (L.) ex. Kurz is an important medicinal plant. Callus induction and regeneration was studied from stem explant of in-vitro grown plant of Rauvolfia serpentina(L.) Benth. ex Kurz (Apocynaceae) on Murashige Skoog (1962) medium supplemented with 1mg/l 2,4-Dichlorophenocy acetic acid (2,4-D) and 1mg/l Kinetin (Kn). Vigorous growth of callus occurs after 4 weeks of culture. Callus was sub-cultured on Murashige and Skoog (MS) medium supplemented with different concentration of 2, 4-D (0.5-3.0 mg/l) and 10% coconut milk. Regeneration of plantlets occurred on MS medium containing 3 mg/1 of 2, 4-D and 10% coconut milk. These plantlets were rooted on MS medium supplemented with 1 mg/l IAA .The regenerated plantlets were able to grow on soil after short period ofacclimatization. Key words: Explant; In-vitro culture; MS medium;  2, 4 Dichlorophenoxy acetic acid; Kinetin; Callus; Tissue culture; Coconut milk. Journal of Natural History Museum Vol. 24, 2009 Page: 82-88

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