scholarly journals Influence of culture medium composition on the micropropagation of Thymus tauricus Klokov et Des.-Shost.

2021 ◽  
Vol 937 (4) ◽  
pp. 042078
Author(s):  
A Tevfik ◽  
N Yegorova
Keyword(s):  
Seed Production ◽  
Root Length ◽  
Culture Medium ◽  
Culture Media ◽  
Medium Composition ◽  
Dwarf Shrub ◽  
Traditional Methods ◽  
Second Stage ◽  
Clonal Micropropagation ◽  
Propagation Stage

Abstract Thymus tauricus Klokov et Des.-Shost. is a perennial dwarf shrub of the Lamiaceae family. This plant has anti-inflammatory, antispasmodic, analgesic and antiseptic properties. The use of biotechnological methods makes it possible to increase the efficiency of traditional methods of breeding and seed production. Our investigation aimed to study the influence of the culture medium composition on the explants morphogenesis at 1-2th stages of Thymus tauricus clonal micropropagation. When comparing three cytokinins, the best explants development was revealed on culture media containing kinetin. Both high vitrification rate of microshoots (31.2-90.2%) and formation of small shoots were observed on media supplemented with BAP or TDZ. The most effective culture medium at the introduction stage is MS with 1.0 mg/l kinetin and 1.0 mg/l GA3 or 1.0 mg/l kinetin. On this medium, on average, 8.4-10.1 microshoots per explant and shoots length of 1.9 cm were obtained. It was found that the optimal culture medium at the actually propagation stage is MS with 1.0 mg/l Kin or 1.0 mg/l kinetin and 0.5 mg/l IAA, on which the multiplication index 26.9-28.5 were obtained. Induction of rhizogenesis with a frequency of 30.2-94.4% and a root length of 1.6-3.0 cm was noted on culture media for the second stage of clonal micropropagation. The results of the studies are the basis for development T. tauricus clonal micropropagation method.

scholarly journals Culture medium for mint micropropagation in vitro

2020 ◽  
Author(s):  
N.A. Yegorova ◽  
◽  
M.S. Zagorskaya ◽  
O.V. Yakimova ◽  
◽  
...  
Keyword(s):  
In Vitro Propagation ◽  
Culture Medium ◽  
Medium Composition ◽  
Multiplication Rate ◽  
Ms Medium ◽  
Second Stage ◽  
Clonal Micropropagation ◽  
Propagation Technique

The influence of the culture medium composition on the development of explants at the second stage of clonal micropropagation of mint (Mentha canadensis L. K59(4n)) was studied in order to improve the in vitro propagation technique. It was shown that the maximum multiplication rate (11.5) was provided by MS medium supplemented with BAP (1.0 mg/L), IAA (0.5 mg/L) and 2% sucrose.

scholarly journals Clonal micropropagation of Thymus vulgaris L.

2020 ◽  
Vol 224 ◽  
pp. 04001
Author(s):  
A Sh Tevfik ◽  
N. A. Yegorova
Keyword(s):  
Plant Material ◽  
Culture Medium ◽  
Culture Media ◽  
Morphometric Parameters ◽  
Thymus Vulgaris ◽  
Ms Medium ◽  
Ex Vitro ◽  
Clonal Micropropagation ◽  
Propagation Stage

Thymus vulgaris L. is one of the widely known spicy aromatic and medicinal plants. Thyme plant material is widely used in medicine, cooking and perfumery. To increase the efficiency of breeding and seed production, it is necessary to develop biotechnological techniques, in particular, clonal micropropagation. The aim of the research is to optimize the composition of culture media for the main stages of propagation in vitro and to select adaptation ex vitro conditions for the development of Thymus vulgaris. clonal micropropagation. The article presents the results of studies of explant morphometric parameters cultivated on 20 variants of culture media at firstsecond stages of micropropagation. It was found that the optimal culture medium at the introduction stage is MS medium with 1.0 mg/l Kin and 1.0 mg/l GA3, on which, on average, 2.2 microshoots per explant with a length of 1.9 cm were obtained. Both high vitrification rate of microshoots and formation of small shoots (0.6-0.9 cm) were observed on media supplemented with BAP or TDZ. The most effective culture medium at the proper propagation stage is MS with 1.0 mg/l Kin, on which 4.6 shoots per explant and the multiplication index 12.8 were obtained. It is advisable to root microshoots at the 3rd stage of micropropagation on MS culture medium supplemented with 1.0 mg/l IBA or 1.0 mg/l IAA. It has been shown that it is possible to obtain high plant survival rate (89.5%) during adaptation ex vitro, using a substrate consisting of peat and perlite (1:1).

scholarly journals Peculiarities of Thymus tauricus Klokov et Des.-Shost. clonal micropropagation

2020 ◽  
Author(s):  
A.Sh. Tevfik ◽  
◽  
N.A. Yegorova ◽  
Keyword(s):  
Culture Medium ◽  
Medium Composition ◽  
Ms Medium ◽  
Optimal Composition ◽  
Cultivation Conditions ◽  
Second Stage ◽  
Clonal Micropropagation

The aim of the investigation was to study the influence of cultivation conditions and the culture medium composition on the Thymus tauricus Klokov et Des.-Shost explants morphogenesis at the 1st-2nd stages of clonal micropropagation. The optimal composition of culture medium at the introduction stage is the MS medium with 1.0 mg/l of kinetin and 1.0 mg/l GА3. To obtain a high multiplication index (29.4) at the second stage of micropropagation, it is necessary to cultivate explants in flasks with MS medium supplemented with 1.0 mg/l kinetin.

Pertumbuhan Dan Perkembangan Anggrek Dendrobium anosmum Pada Media Kultur In Vitro Dengan Beberapa Konsentrasi Air Kelapa

Agrologia ◽  
2018 ◽  
Vol 1 (1) ◽  
Author(s):  
S. Tuhuteru ◽  
Meity L Hehanussa ◽  
Simon H.T Raharjo
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Water Concentration ◽  
Medium Composition ◽  
Coconut Water ◽  
Orchid Species ◽  
Randomized Design ◽  
Wet Weight ◽  
Completely Randomized Design

Dendrobium anosmum is one of natural orchids in Indonesia. Optimization of medium composition for orchid propagation through in vitro culture is necessary to enhance propagule multiplication capabilities and quality. This study was aimed to study the influence of concentration of coconut water in culture medium on in vitro growth and development of D. anosmum orchid species and to determine the optimal coconut water concentration in culture media.  The experiment were arranged in a Completely Randomized Design with four treatments and eight replications. The treatments consisted of the addition of coconut water with concentrations: 0 ml•l -1 (control), 50 ml•l-1, 100 ml•l-1 and 150 ml•l-1. The results showed that addition of coconut water in culture medium gave different effect on shoot growth and multiplication of D. anosmum orchids.  Coconut water concentration of 100 ml•l-1 was the best concentration for growth and multiplication of D. anosmum orchids, based on both shoots and roots growth, plantlet height and wet weight.

scholarly journals In vitro organogenesis in some citrus species

2011 ◽  
Vol 33 (2) ◽  
pp. 526-531 ◽  
Author(s):  
Evandro Henrique Schinor ◽  
Fernando Alves de Azevedo ◽  
Francisco de Assis Alves Mourão Filho ◽  
Beatriz Madalena Januzzi Mendes
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Adventitious Shoots ◽  
Medium Composition ◽  
Naphthaleneacetic Acid ◽  
In Vitro Organogenesis ◽  
Basal Culture Medium ◽  
Medium Supplementation ◽  
Media Supplementation

In vitro organogenesis of Citrus was studied for the genotypes Citrus sinensis cv. 'Natal', C. limonia, C. volkameriana, and C. aurantium, with the use of epicotyl segments-derived explants, cultured in MT salts and vitamins medium supplemented with different concentrations of 6-benzylaminopurine (BAP - 0.0; 0.5; 1.0; 1.5 or 2.0 mg L-1). For the recalcitrant genotypes C. limonia and C. aurantium the in vitro organogenesis was also studied with internodal segments-derived explants, cultured in MT salts and vitamins medium supplemented with 0; 0.5; 1.0; 2.0, or 4.0 mg L-1 of BAP. The efficiency of culture medium supplementation with the combination of BAP (0.0; 1.0, or 2.0 mg L-1) and NAA (1-naphthaleneacetic acid - 0.0; 0.3, or 0.5 mg L-1) in the development of adventitious shoots was evaluated for C. aurantium. Culture medium supplementation with BAP is not essential for the adventitious shoots development in the four genotypes studied when epicotyl segments-derived explants are used. In general, culture media supplementation with BAP decreased the percentage of responsive explants excepted for C. sinensis cv. 'Natal' and C. limonia when the concentrations of 1.5 and 2.0 mg/L were used. The presence of cytokinin, in concentrations up to 2 mg/L, stimulated the in vitro organogenesis when internodal segments-derived explants were used for C. limonia and C. aurantium. For C. aurantium no adventitious shoots developed in explants (internodal segments) cultured in basal culture medium, without BAP supplementation. Although no statistic differences could be detected, culture media supplementation with the combination of BAP and NAA favored the development of adventitious shoots in C. aurantium. The best concentration of NAA varied according to BAP concentration. The results presented herein, show that Citrus in vitro organogenesis depends on the interaction of culture medium composition, explant differentiation level, and genotype.

scholarly journals Clonal Micropropagation of representatives of the genus Dactylorhiza Neck. ex Nevski

2021 ◽  
Vol 4 (46) ◽  
pp. 17-17
Author(s):  
Alexander Saakian ◽  
◽  
Keyword(s):  
Survival Rate ◽  
Growth Regulators ◽  
In Vitro Propagation ◽  
Culture Medium ◽  
Culture Media ◽  
Ms Medium ◽  
Organic Additives ◽  
Clonal Micropropagation ◽  
Half Strength

Abstract The aim of this study is to develop and improve methods of in vitro propagation of representatives of Dactylorhiza: D.baltica , D. fuchsii. For the study, we used protocorms obtained by the asymbiotic germination of seed during 90 days. It has been established that half-strength of Murashige and Skoog (1962) medium (½ MS) supplemented with 1-2 mg/l 6-Benzylaminopurine(6-BAP), potato puree (20g/l), and charcoal (1g/l) effectively influenced the development of protocorms, and seedlings formation in the studied species. The result of the study showed that the survival rate of protocorms was high in all experimental culture media, but in D. fuchsii it was better at a concentration 2mg/l of 6-BAP (95.4%), while in D. baltica it was high at 1mg/l (87.0%). The highest percentage of multiple protocorms (68%) and the formation of new secondary protocorms in D. fuchsii (5,5±0,3 units) were observed on a culture medium containing 2 mg/l 6-BAP. The highest percent of rooting of D. fuchsii protosoms (78%) and length of roots (0.9cm) observed in ½ MS medium without growth regulators. During the development of D. baltica protosoms, the culture medium of ½ MS containing 1 mg/l 6-BAP had the best effect on the number of roots (1.8±0.1root/protosom), while the medium supplemented with 2mg/l of 6-BAP contributed to the formation of a larger number of new secondary protocorms (3,2±0,1protocorm/unit). During the subsequent cultivation of protosoms of D. baltica on a culture medium containing 1 mg/l it was observed an increase in the height of shoots (4,8±0,3 см), and the length of roots (2,2±0,1 см), wherein the number of newly formed protocorms was higher by 30% on the medium supplemented with 2 mg/l 6-BAP. Keywords: DACTYLORHIZA BALTICA, DACTYLORHIZA FUCHSII, IN VITRO, PROTOCORMS, ORGANIC ADDITIVES

Improved blood culture medium for radiometric detection of yeasts

1979 ◽  
Vol 9 (3) ◽  
pp. 448-449
Author(s):  
R L Hopfer ◽  
K Mills ◽  
D Gröschel
Keyword(s):  
Blood Culture ◽  
Culture Medium ◽  
Culture Media ◽  
Medium Composition ◽  
Co2 Production ◽  
Yeast Nitrogen Base ◽  
Nitrogen Base ◽  
System A ◽  
Radiometric Detection ◽  
Triton X

The influence of medium composition on the rate of CO2 production by yeasts in simulated blood cultures was determined in the Bactec radiometric system. A yeast nitrogen base broth containing [14C]glucose and Triton X-100 allowed the detection of positive cultures earlier than the regular Bactec blood culture media.

scholarly journals Optimization Studies of Culture Media for In-Vitro Clonal Micropropagation of New Grape Varieties

2021 ◽  
Author(s):  
Abdulmalik Batukaev ◽  
Eliza Sobralieva ◽  
Diana Palaeva
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
In Vitro Regeneration ◽  
Mineral Salts ◽  
Additional Increase ◽  
Shoot Bud ◽  
Clonal Micropropagation ◽  
Aseptic Culture ◽  
Grape Varieties

This article describes the effect of Gautheret, White, Heller and Murashige & Skoog mineral salts during in-vitro clonal micropropagation of new grape varieties. The optimal mineral compositions of the culture medium that support the in-vitro regeneration of isolated grape explants were identified. The grapes that were studied were the Bart and Augustine varieties. Primary grape explants were cultivated for 30 days in a non-transplanted culture. Increased regenerative activity was observed in the Murashige & Skoog and White media. Increased haemogenesis occurred and shoots regenerated. The addition of cytokinin 6-BAP to the medium for obtaining aseptic culture led to an increase in the frequency of shoot-bud production by 5 to 6 times, depending on the type of medium. Combining 6-BAP with the auxin NAA provided an additional increase in the frequency of shoot-bud production, but to a lesser extent. Adding growth regulators to the culture medium also reduced the frequency of explant necrosis. Keywords: grapes, mineral salts, culture medium, microclonal propagation, in-vitro, cytokinins, auxins

Tomato culture filtrate stimulates hatching and activity of Meloidogyne incognita juveniles

Nematology ◽  
2009 ◽  
Vol 11 (1) ◽  
pp. 51-61 ◽  
Author(s):  
Takayuki Mizukubo ◽  
Yuji Oka
Keyword(s):  
Meloidogyne Incognita ◽  
Laboratory Experiments ◽  
Culture Medium ◽  
Culture Media ◽  
Nematode Control ◽  
Second Stage ◽  
Plant Culture ◽  
Fresh Culture Medium ◽  
Fresh Culture ◽  
Tomato Culture

AbstractHydroponic culture media were tested after growing tomato, okra, cucumber and bean for their effect on hatching and mobility of Meloidogyne incognita second-stage juveniles (J2) in laboratory experiments. Tomato and okra culture media increased the numbers of J2 that hatched as compared to those in water or fresh culture medium. The tomato culture medium increased hatching even in the presence of fosthiazate, an organophosphate nematicide, and at a concentration that inhibited hatching in the absence of tomato culture medium. Neither heat treatment of the tomato culture medium nor change of pH abolished its hatching stimulatory activity. When active J2 were incubated in the tomato culture media, the percentages of nematodes that became quiescent were lower than those of nematodes incubated in water or in fresh culture medium for 3 and 7 days in two trials. Moreover, the sigmoid movement of J2 was faster in the tomato culture medium than in water. Quiescent J2 became active more rapidly in culture media of tomato, cucumber and okra than in water or fresh culture medium. In experiments using soil containing quiescent J2, more J2 were extracted with the Baermann funnel method from soil treated with tomato culture medium than from soil treated with water. The results confirm that nematode stimulants, which may serve as a potential means in nematode control, exist in the plant culture media.

scholarly journals Adjustment of culture media to optimize in vitro rhizogenesis of domestic plums of various origins

2021 ◽  
Vol 34 ◽  
pp. 03001
Author(s):  
Natalia Kovalenko ◽  
Nadezhda Polivara
Keyword(s):  
Nutrient Medium ◽  
Culture Medium ◽  
Root Formation ◽  
Culture Media ◽  
Medium Composition ◽  
Ms Medium ◽  
Growth Substances ◽  
Nutrient Media ◽  
Plum Varieties

This paper presents the results of the adjustment of 18 modifications of culture medium based on MS medium composition – Murashige and Skoog (1962) for in vitro rhizogenesis of 10 varieties of domestic plum. There were used 4 nutrient media with a full amount of macro- and microelements – MS1 with a different combination of phytohormones, 1 medium without hormones – MSc, 13 media – MS2 with half the amount of macronutrients, differing in hormonal composition. It was found that the maximum number of rooted microshoots from 21.2 to 52.2 % was on MS2–8 medium, from 11.2 to 43.1 % on MS2–5. The analysis showed that into the medium increases the percentage of microplants by 12-17 % comparing with the medium MS2–5 and MS2–7 without FA. The distinctiveness of nutrient media by the type of auxin (IAA, NAA, IBA) made it possible to clarify that IBA is the most optimal of the auxins, and the concentration of 1.7 mg/l is borderline for the growth reactions of plum varieties. It was revealed that in vitro root formation depends not only on the compositions of growth substances in the nutrient medium, but also on the genotype of the variety.

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