Adjustment of culture media to optimize in vitro rhizogenesis of domestic plums of various origins

This paper presents the results of the adjustment of 18 modifications of culture medium based on MS medium composition – Murashige and Skoog (1962) for in vitro rhizogenesis of 10 varieties of domestic plum. There were used 4 nutrient media with a full amount of macro- and microelements – MS1 with a different combination of phytohormones, 1 medium without hormones – MSc, 13 media – MS2 with half the amount of macronutrients, differing in hormonal composition. It was found that the maximum number of rooted microshoots from 21.2 to 52.2 % was on MS2–8 medium, from 11.2 to 43.1 % on MS2–5. The analysis showed that into the medium increases the percentage of microplants by 12-17 % comparing with the medium MS2–5 and MS2–7 without FA. The distinctiveness of nutrient media by the type of auxin (IAA, NAA, IBA) made it possible to clarify that IBA is the most optimal of the auxins, and the concentration of 1.7 mg/l is borderline for the growth reactions of plum varieties. It was revealed that in vitro root formation depends not only on the compositions of growth substances in the nutrient medium, but also on the genotype of the variety.

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Pertumbuhan Dan Perkembangan Anggrek Dendrobium anosmum Pada Media Kultur In Vitro Dengan Beberapa Konsentrasi Air Kelapa

Agrologia ◽

10.30598/a.v1i1.293 ◽

2018 ◽

Vol 1 (1) ◽

Author(s):

S. Tuhuteru ◽

Meity L Hehanussa ◽

Simon H.T Raharjo

Keyword(s):

Culture Medium ◽

Culture Media ◽

Water Concentration ◽

Medium Composition ◽

Coconut Water ◽

Orchid Species ◽

Randomized Design ◽

Wet Weight ◽

Completely Randomized Design

Dendrobium anosmum is one of natural orchids in Indonesia. Optimization of medium composition for orchid propagation through in vitro culture is necessary to enhance propagule multiplication capabilities and quality. This study was aimed to study the influence of concentration of coconut water in culture medium on in vitro growth and development of D. anosmum orchid species and to determine the optimal coconut water concentration in culture media. The experiment were arranged in a Completely Randomized Design with four treatments and eight replications. The treatments consisted of the addition of coconut water with concentrations: 0 ml•l -1 (control), 50 ml•l-1, 100 ml•l-1 and 150 ml•l-1. The results showed that addition of coconut water in culture medium gave different effect on shoot growth and multiplication of D. anosmum orchids. Coconut water concentration of 100 ml•l-1 was the best concentration for growth and multiplication of D. anosmum orchids, based on both shoots and roots growth, plantlet height and wet weight.

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Culture medium for mint micropropagation in vitro

PROCEEDINGS OF V INTERNATIONAL SCIENTIFIC CONFERENCE “CURRENT STATE, PROBLEMS AND PROSPECTS OF THE DEVELOPMENT OF AGRARIAN SCIENCE” ◽

10.33952/2542-0720-2020-5-9-10-88 ◽

2020 ◽

Author(s):

N.A. Yegorova ◽

◽

M.S. Zagorskaya ◽

O.V. Yakimova ◽

◽

...

Keyword(s):

In Vitro Propagation ◽

Culture Medium ◽

Medium Composition ◽

Multiplication Rate ◽

Ms Medium ◽

Second Stage ◽

Clonal Micropropagation ◽

Propagation Technique

The influence of the culture medium composition on the development of explants at the second stage of clonal micropropagation of mint (Mentha canadensis L. K59(4n)) was studied in order to improve the in vitro propagation technique. It was shown that the maximum multiplication rate (11.5) was provided by MS medium supplemented with BAP (1.0 mg/L), IAA (0.5 mg/L) and 2% sucrose.

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The influence of the nutrient medium composition on the induction of calluso- and morphogenesis of Melissa officinalis L.

PROCEEDINGS OF V INTERNATIONAL SCIENTIFIC CONFERENCE “CURRENT STATE, PROBLEMS AND PROSPECTS OF THE DEVELOPMENT OF AGRARIAN SCIENCE” ◽

10.33952/2542-0720-2020-5-9-10-100 ◽

2020 ◽

Author(s):

O.V. Yakimova ◽

◽

N.A. Yegorova ◽

Keyword(s):

Callus Induction ◽

Nutrient Medium ◽

Culture Medium ◽

Medium Composition ◽

Ms Medium ◽

Explant Type ◽

Maximum Frequency ◽

Melissa Officinalis ◽

Exogenous Factors

The features of the calluso- and morphogenesis induction during the cultivation of tissues and organs of Melissa officinalis depending on endogenous and exogenous factors were revealed. The maximum frequency of callus induction (59.5–92.9 %) was noted on the MS medium with 1.0 mg/l 2.4-D and 0.5 mg/l BAP. The induction of morphogenesis from callus was influenced by the composition of the culture medium, the explant type and cultivar. The maximum frequency of morphogenesis induction (20.0–28.0 % depending on the cultivar) from callus was noted on MS culture medium supplemented with 1.0 mg/L NAA and 0.5 mg/L BAP or 1.0 mg/L NAA and 0.5 mg L TDZ.

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In vitro organogenesis in some citrus species

Revista Brasileira de Fruticultura ◽

10.1590/s0100-29452011005000050 ◽

2011 ◽

Vol 33 (2) ◽

pp. 526-531 ◽

Cited By ~ 2

Author(s):

Evandro Henrique Schinor ◽

Fernando Alves de Azevedo ◽

Francisco de Assis Alves Mourão Filho ◽

Beatriz Madalena Januzzi Mendes

Keyword(s):

Culture Medium ◽

Culture Media ◽

Adventitious Shoots ◽

Medium Composition ◽

Naphthaleneacetic Acid ◽

In Vitro Organogenesis ◽

Basal Culture Medium ◽

Medium Supplementation ◽

Media Supplementation

In vitro organogenesis of Citrus was studied for the genotypes Citrus sinensis cv. 'Natal', C. limonia, C. volkameriana, and C. aurantium, with the use of epicotyl segments-derived explants, cultured in MT salts and vitamins medium supplemented with different concentrations of 6-benzylaminopurine (BAP - 0.0; 0.5; 1.0; 1.5 or 2.0 mg L-1). For the recalcitrant genotypes C. limonia and C. aurantium the in vitro organogenesis was also studied with internodal segments-derived explants, cultured in MT salts and vitamins medium supplemented with 0; 0.5; 1.0; 2.0, or 4.0 mg L-1 of BAP. The efficiency of culture medium supplementation with the combination of BAP (0.0; 1.0, or 2.0 mg L-1) and NAA (1-naphthaleneacetic acid - 0.0; 0.3, or 0.5 mg L-1) in the development of adventitious shoots was evaluated for C. aurantium. Culture medium supplementation with BAP is not essential for the adventitious shoots development in the four genotypes studied when epicotyl segments-derived explants are used. In general, culture media supplementation with BAP decreased the percentage of responsive explants excepted for C. sinensis cv. 'Natal' and C. limonia when the concentrations of 1.5 and 2.0 mg/L were used. The presence of cytokinin, in concentrations up to 2 mg/L, stimulated the in vitro organogenesis when internodal segments-derived explants were used for C. limonia and C. aurantium. For C. aurantium no adventitious shoots developed in explants (internodal segments) cultured in basal culture medium, without BAP supplementation. Although no statistic differences could be detected, culture media supplementation with the combination of BAP and NAA favored the development of adventitious shoots in C. aurantium. The best concentration of NAA varied according to BAP concentration. The results presented herein, show that Citrus in vitro organogenesis depends on the interaction of culture medium composition, explant differentiation level, and genotype.

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Clonal Micropropagation of representatives of the genus Dactylorhiza Neck. ex Nevski

АгроЭкоИнфо ◽

10.51419/20214417 ◽

2021 ◽

Vol 4 (46) ◽

pp. 17-17

Author(s):

Alexander Saakian ◽

◽

Keyword(s):

Survival Rate ◽

Growth Regulators ◽

In Vitro Propagation ◽

Culture Medium ◽

Culture Media ◽

Ms Medium ◽

Organic Additives ◽

Clonal Micropropagation ◽

Half Strength

Abstract The aim of this study is to develop and improve methods of in vitro propagation of representatives of Dactylorhiza: D.baltica , D. fuchsii. For the study, we used protocorms obtained by the asymbiotic germination of seed during 90 days. It has been established that half-strength of Murashige and Skoog (1962) medium (½ MS) supplemented with 1-2 mg/l 6-Benzylaminopurine(6-BAP), potato puree (20g/l), and charcoal (1g/l) effectively influenced the development of protocorms, and seedlings formation in the studied species. The result of the study showed that the survival rate of protocorms was high in all experimental culture media, but in D. fuchsii it was better at a concentration 2mg/l of 6-BAP (95.4%), while in D. baltica it was high at 1mg/l (87.0%). The highest percentage of multiple protocorms (68%) and the formation of new secondary protocorms in D. fuchsii (5,5±0,3 units) were observed on a culture medium containing 2 mg/l 6-BAP. The highest percent of rooting of D. fuchsii protosoms (78%) and length of roots (0.9cm) observed in ½ MS medium without growth regulators. During the development of D. baltica protosoms, the culture medium of ½ MS containing 1 mg/l 6-BAP had the best effect on the number of roots (1.8±0.1root/protosom), while the medium supplemented with 2mg/l of 6-BAP contributed to the formation of a larger number of new secondary protocorms (3,2±0,1protocorm/unit). During the subsequent cultivation of protosoms of D. baltica on a culture medium containing 1 mg/l it was observed an increase in the height of shoots (4,8±0,3 см), and the length of roots (2,2±0,1 см), wherein the number of newly formed protocorms was higher by 30% on the medium supplemented with 2 mg/l 6-BAP. Keywords: DACTYLORHIZA BALTICA, DACTYLORHIZA FUCHSII, IN VITRO, PROTOCORMS, ORGANIC ADDITIVES

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Designing a Nutrient Medium for the Accumulation of Microbial Mass of Listeria

Acta biomedica scientifica ◽

10.29413/abs.2020-5.4.8 ◽

2020 ◽

Vol 5 (4) ◽

pp. 60-66

Author(s):

N. M. Khaptanova ◽

S. V. Lukyanova ◽

V. I. Kuznetsov ◽

N. G. Gefan ◽

N. M. Аndreevskaya ◽

...

Keyword(s):

Nutrient Medium ◽

Culture Medium ◽

Specific Activity ◽

Germination Rate ◽

Biochemical Properties ◽

Quantitative Composition ◽

Nutrient Media ◽

Microbiological Methods ◽

Microbial Mass

Background. To obtain reliable results of laboratory studies on the identification of Listeria, the presence of certified diagnostic agglutinating Listeria sera is required. An important step in the manufacturing process of such medical devices for in vitro diagnostics requires effective nutrient media for the accumulation of listeriosis microbe. Aim of the research. To develop an effective nutrient medium for the accumulation of bacterial mass of Listeria. Materials and methods. The object of the study was an experimental culture medium for Listeria cultivation. As a control, we used nutrient agar for the cultivation of microorganisms (fish meal hydrolysate, FMH-agar) and meat-peptone agar with 1 % glucose (MPA with 1 % glucose). The specific activity of nutrient media during cultivation of the test strain Listeria monocytogenes 766 was evaluated using a complex of microbiological methods. Results. The optimal base of the nutrient medium for Listeria cultivation has been selected: pancreatic hydrolysate of river magpie fish (Rutilus rutilus lacustris) and hydrolysate of meat water production waste. The qualitative and quantitative composition of the nutrient medium has been developed, its physical, chemical and biological properties have been studied. It was found that after 24 hours of incubation at 37 °C, the nutrient medium provided the growth of typical Listeria colonies. The germination rate was 85 %, which is higher compared to the growth of the culture on MPA with 1 % glucose and GRM agar by an average of 21 % (p < 0,05). Conclusion. The experimental culture medium for Listeria cultivation provided growth of colonies of the test strain L. monocytogenes 766 with the preservation of characteristic cultural, morphological and biochemical properties, and, in terms of germination and growth rate, exceeded the control media. The developed nutrient medium provides effective growth of Listeria and can be used as a medium for the accumulation of microbial mass.

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Clonal micropropagation of Thymus vulgaris L.

E3S Web of Conferences ◽

10.1051/e3sconf/202022404001 ◽

2020 ◽

Vol 224 ◽

pp. 04001

Author(s):

A Sh Tevfik ◽

N. A. Yegorova

Keyword(s):

Plant Material ◽

Culture Medium ◽

Culture Media ◽

Morphometric Parameters ◽

Thymus Vulgaris ◽

Ms Medium ◽

Ex Vitro ◽

Clonal Micropropagation ◽

Propagation Stage

Thymus vulgaris L. is one of the widely known spicy aromatic and medicinal plants. Thyme plant material is widely used in medicine, cooking and perfumery. To increase the efficiency of breeding and seed production, it is necessary to develop biotechnological techniques, in particular, clonal micropropagation. The aim of the research is to optimize the composition of culture media for the main stages of propagation in vitro and to select adaptation ex vitro conditions for the development of Thymus vulgaris. clonal micropropagation. The article presents the results of studies of explant morphometric parameters cultivated on 20 variants of culture media at firstsecond stages of micropropagation. It was found that the optimal culture medium at the introduction stage is MS medium with 1.0 mg/l Kin and 1.0 mg/l GA3, on which, on average, 2.2 microshoots per explant with a length of 1.9 cm were obtained. Both high vitrification rate of microshoots and formation of small shoots (0.6-0.9 cm) were observed on media supplemented with BAP or TDZ. The most effective culture medium at the proper propagation stage is MS with 1.0 mg/l Kin, on which 4.6 shoots per explant and the multiplication index 12.8 were obtained. It is advisable to root microshoots at the 3rd stage of micropropagation on MS culture medium supplemented with 1.0 mg/l IBA or 1.0 mg/l IAA. It has been shown that it is possible to obtain high plant survival rate (89.5%) during adaptation ex vitro, using a substrate consisting of peat and perlite (1:1).

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In vitro Dendrobium nobile plant growth and rooting in different sucrose concentrations

Horticultura Brasileira ◽

10.1590/s0102-05362004000400023 ◽

2004 ◽

Vol 22 (4) ◽

pp. 780-783 ◽

Cited By ~ 18

Author(s):

Ricardo T. de Faria ◽

Fabiana N. Rodrigues ◽

Luciana do V.R. Oliveira ◽

Cláudio Müller

Keyword(s):

Plant Growth ◽

Culture Medium ◽

Culture Media ◽

Sucrose Concentration ◽

Ms Medium ◽

In Vitro Growth ◽

Dendrobium Nobile ◽

Modified Ms Medium ◽

Dendrobium Nobile Lindl

Sucrose is a very important component in in vitro culture media, serving as a source of carbon and energy. In this paper, the rooting and in vitro growth of Dendrobium nobile Lindl (Orchidaceae) were studied using different sucrose concentrations (0 g L-1; 5 g L-1; 10 g L-1; 20 g L-1; 30 g L-1 and 60 g L-1), in a modified MS medium containing half the regular concentration of macronutrients at pH 5.8. Greater increases in plant height (4.21±0.6 cm) and high seedling multiplication (1:4) were observed in the 60 g L-1 sucrose treatment, even without the addition of plant hormones. Sucrose concentration in the culture medium did not influence in vitro plant rooting.

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RESPONSE OF Cattleya forbesii ORCHID TO INCREASING SILICON CONCENTRATIONS IN VITRO

Revista Caatinga ◽

10.1590/1983-21252016v29n103rc ◽

2016 ◽

Vol 29 (1) ◽

pp. 18-24 ◽

Cited By ~ 4

Author(s):

RONAN CARLOS COLOMBO ◽

VANESSA FAVETTA ◽

RICARDO TADEU DE FARIA ◽

FELIPE ARANHA DE ANDRADE ◽

VANDERLI MARINO MELEM

Keyword(s):

Culture Medium ◽

Culture Media ◽

Average Length ◽

Ms Medium ◽

In Vitro Growth ◽

Shoot Height ◽

Leaf Tissues ◽

Number Of Leaves ◽

Scanning Electron

ABSTRACT: Addition of Silicon (Si) to culture media has been shown to improve the development of seedlings grown in vitro, and to reduce losses during the acclimatization phase. The objective of this study was to evaluate the in vitro growth of Cattleya forbesii (Orchidaceae) in MS medium containing five different concentrations of SiO2 (0.0, 0.5, 1.0, 1.5, and 2.0 g·L−1). At day 200, the following variables were measured: number of roots, average length of the root system, leaf area, number of leaves and shoots, shoot height, fresh and dry masses of roots and shoots, water content of roots and shoots, and pH of the culture medium. Most variables decreased as the concentration of Si increased, reducing the in vitro vegetative growth of C. forbesii. Accumulation of Si in leaf tissues was detected by scanning electron microscopy, confirming uptake by plants. The Si source and concentrations tested showed no beneficial effect on in vitro growth of C. forbesii.

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Multiplicação de anador (Justicia pectoralis) in vitro

Revista Verde de Agroecologia e Desenvolvimento Sustentável ◽

10.18378/rvads.v11i3.3986 ◽

2016 ◽

Vol 11 (3) ◽

pp. 159 ◽

Cited By ~ 1

Author(s):

Rômulo Magno Oliveira Freitas ◽

Narjara Walessa Nogueira ◽

Sidney Carlos Praxedes

Keyword(s):

Plant Material ◽

In Vitro Propagation ◽

Culture Medium ◽

Culture Media ◽

Statistical Design ◽

The Other ◽

Nodal Segments ◽

Ms Medium ◽

Number Of Leaves

O trabalho teve como objetivo desenvolver um protocolo de micropropagação de segmentos nodais de anador (Justicia pectoralis). Para isso foram realizados dois experimentos. O delineamento estatístico utilizado foi o inteiramente casualizado, com 15 repetições. Os segmentos de J. pectoralis, após desinfestados, foram cultivados em meio MS durante 30 dias. No primeiro experimento, esse material foi repicado em três meios de cultura (MS, WPM e B5) e após 77 dias foram avaliados comprimento de plântula, número de raízes, número de folhas e o número de segmentos nodais. Para o segundo experimento foram testadas duas citocininas (BAP e Cinetina) nas seguintes dosagens 0,0; 0,5; 5,0 e 20,0 mM. Aos 60 dias após a repicagem foram avaliadas as seguintes características: números de folhas, número de raízes e número de explantes por planta. O meio MS foi o que apresentou maior comprimento de plântula. As demais variáveis não diferiram entre os meios utilizados. Por isso o meio MS foi utilizado para o segundo experimento onde se verificou que a utilização de BAP proporcionou maior número de folhas e de explantes quando submetido à concentração de 20 mM. Dessa forma, para multiplicação de seg Justicia pectoralis, recomenda-se a utilização de meio MS com adição de 20mM de BAP.In vitro propagation of Justicia pectoralisAbstract: The study aimed to establish a micropropagation protocol for Justicia pectoralis nodal segments. Two experiments were conducted. The statistical design was the completely randomized with 15 repetitions. After disinfestation, the segments of J. pectoralis were inoculated in the MS culture medium for 30 days. In the first experiment, the plant material was transferred to three culture media (MS, WPM and B5). The length of seedlings, number of roots, number of leaves, and number of nodal segments were evaluated at 77 days after transferring. In the second experiment two cytokinins (BAP and Kinetin) were tested in the following concentrations: 0.0; 0.5; 5.0 and 20.0 mM. At 60 days after transplanting the number of leaves, number of roots and number of explants per plant were evaluated. The MS medium induced the highest length of seedlings, but there was no effect for the other variables. Therefore, this medium was used for the second experiment, when it was found that BAP induced a larger number of leaves and explants when applied at 20 mM. Therefore, for multiplying J. pectoralis nodal segments we recommend the use of MS medium with 20 mM BAP.

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