Search for Genetic Variants in the Retinoid X Receptor-γ-Gene by Polymerase Chain Reaction-Single-Strand Conformation Polymorphism in Patients with Resistance to Thyroid Hormone without Mutations in Thyroid Hormone Receptor β Gene

Thyroid ◽  
2004 ◽  
Vol 14 (5) ◽  
pp. 355-358 ◽  
Author(s):  
Stefano Romeo ◽  
Claudia Menzaghi ◽  
Rocco Bruno ◽  
Federica Sentinelli ◽  
Mara Fallarino ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Thyroid Hormone ◽  
Genetic Variants ◽  
Thyroid Hormone Receptor ◽  
Single Strand Conformation Polymorphism ◽  
Retinoid X Receptor ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Thyroid Hormone Receptor Β ◽  
Conformation Polymorphism

Thyroid hormone receptor β mRNA expression in Sertoli cells isolated from prepubertal testis

1995 ◽  
Vol 14 (1) ◽  
pp. 131-134 ◽  
Author(s):  
S Palmero ◽  
P De Marco ◽  
E Fugassa
Keyword(s):  
Polymerase Chain Reaction ◽  
Thyroid Hormone ◽  
Sertoli Cell ◽  
Sertoli Cells ◽  
Binding Sites ◽  
Hormone Receptor ◽  
Thyroid Hormone Receptor ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Thyroid Hormone Receptor Β

ABSTRACT A polymerase chain reaction (PCR)-based assay was used to evaluate the expression of thyroid hormone receptor β mRNA in Sertoli cells isolated from both prepubertal rat and piglet testes. The expression of an mRNA coding for the functional thyroid hormone receptor β isoform, as established by the PCR assay, agrees with the presence of specific tri-iodothyronine (T3)-binding sites in the Sertoli cell nuclei of both species, as previously evaluated by displacement analysis. The results ratify the existence of a functional T3 receptor in the prepubertal testis and confirm the Sertoli cell as a specific target for thyroid hormone action on the developing testis. On the other hand, in both peripubertal rat (Palmero et al. 1988; Jannini et al. 1990) and piglet (Palmero et al. 1992) testes, high-affinity, low-capacity T3 binding sites have been specifically localised at the Sertoli cell level and TRal mRNA expression has been detected very recently in immature Sertoli cells (Jannini et al. 1994). The aim of the present work was to test if, in prepubertal Sertoli cells isolated from both immature rat and piglet testes, the expression of an erbAβ mRNA specifically coding for the TR protein could be detected employing an highly sensitive polymerase chain reaction (PCR)-based assay.

Rapid identification of the common apo E isoform genotype using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP)

1994 ◽  
Vol 8 (1) ◽  
pp. 51-54 ◽  
Author(s):  
Ryoji Aozaki ◽  
Ryuji Kawaguchi ◽  
Utako Ogasa ◽  
Kazumasa Hikiji ◽  
Nobuhiko Kubo ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Single Strand Conformation Polymorphism ◽  
Rapid Identification ◽  
Single Strand ◽  
Chain Reaction ◽  
Apo E ◽  
The Common ◽  
Polymerase Chain ◽  
Conformation Polymorphism

DNA-Based Typing of Human Platelet Antigen Systems by Polymerase Chain Reaction- Single-Strand Conformation Polymorphism Method

Vox Sanguinis ◽  
1995 ◽  
Vol 69 (4) ◽  
pp. 347-351
Author(s):  
Koki Fujiwara ◽  
Katsushi Tokunaga ◽  
Kazumi Isa ◽  
Masaki Miyamoto ◽  
Li Wang ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Human Platelet ◽  
Single Strand Conformation Polymorphism ◽  
Single Strand ◽  
Chain Reaction ◽  
Platelet Antigen ◽  
Human Platelet Antigen ◽  
Polymerase Chain ◽  
Conformation Polymorphism

A963G single nucleotide variant of BMP15 is not common bio-marker for fecundity in goat

2016 ◽  
Author(s):  
Guang-Xin E ◽  
Yong-Fu Huang ◽  
Jian-Ning He ◽  
Wei-Wei Ni ◽  
Yong-Ju Zhao
Keyword(s):  
Polymerase Chain Reaction ◽  
Single Strand Conformation Polymorphism ◽  
Single Nucleotide Variant ◽  
Chain Reaction ◽  
Single Nucleotide ◽  
Exon 2 ◽  
Morphogenetic Protein ◽  
Polymerase Chain ◽  
Bone Morphogenetic Protein 15 ◽  
Conformation Polymorphism

Bone morphogenetic protein 15 (BMP15) is crucial factor for ovulation as well as for increasing litter size. In the present investigation efforts had been carried out to assess the genetic variations in Exon 2 region of BMP15 in goat, using polymerase chain reaction single strand conformation polymorphism (PCR-SSCP) sequencing methods and cooperated frequency distribution to discuss its possibility of related fecundity. Across the 144samples from six breeds were identified in the A963G location of BMP15 using PCR-SSCP and sequences technology. A963A genotype was the most frequent (85.4%) and G963G was the least frequent with a frequency of 4.2% and A963G is 10.4%. It revealed non significant different between high and low fecundity breed. Therefore, this single nucleotide variant is not common Bio-Marker for fecundity in Goat.

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