scholarly journals High-Throughput Phenotyping of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes and Neurons Using Electric Field Stimulation and High-Speed Fluorescence Imaging

2017 ◽  
Vol 15 (4) ◽  
pp. 178-188 ◽  
Author(s):  
Neil J. Daily ◽  
Zhong-Wei Du ◽  
Tetsuro Wakatsuki
Keyword(s):  
Stem Cell ◽  
Electric Field ◽  
Fluorescence Imaging ◽  
High Throughput ◽  
High Speed ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Field Stimulation ◽  
High Throughput Phenotyping ◽  
Induced Pluripotent

High-throughput screening of human induced pluripotent stem cell-derived brain organoids

2020 ◽  
Vol 335 ◽  
pp. 108627 ◽  
Author(s):  
Madel Durens ◽  
Jonathan Nestor ◽  
Madeline Williams ◽  
Kevin Herold ◽  
Robert F. Niescier ◽  
...  
Keyword(s):  
Stem Cell ◽  
High Throughput ◽  
High Throughput Screening ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Induced Pluripotent

scholarly journals A high-throughput screening platform for pigment regulating agents using pluripotent stem cell derived melanocytes

2020 ◽  
Author(s):  
Valentin Parat ◽  
Brigitte Onteniente ◽  
Julien Maruotti
Keyword(s):  
Stem Cell ◽  
High Throughput ◽  
High Throughput Screening ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Light Scatter ◽  
Chemical Treatments ◽  
Human Melanocytes ◽  
Screening Platform ◽  
Induced Pluripotent

AbstractIn this study, we describe a simple and straight-forward assay using induced pluripotent stem cell derived melanocytes and high-throughput flow cytometry, to screen and identify pigment regulating agents. The assays is based on the correlation between forward light-scatter characteristics and melanin content, with pigmented cells displaying high light absorption/low forward light-scatter, while the opposite is true for lowly pigmented melanocytes, as a result of genetic background or chemical treatments. Orthogonal validation is then performed by regular melanin quantification. Such approach was validated using a set of 80 small molecules, and yielded a confirmed hit. The assay described in this study may prove a useful tool to identify modulators of melanogenesis in human melanocytes.

Embryonic and induced pluripotent stem cell staining and sorting with the live-cell fluorescence imaging probe CDy1

2011 ◽  
Vol 6 (7) ◽  
pp. 1044-1052 ◽  
Author(s):  
Nam-Young Kang ◽  
Seong-Wook Yun ◽  
Hyung-Ho Ha ◽  
Sung-Jin Park ◽  
Young-Tae Chang
Keyword(s):  
Stem Cell ◽  
Fluorescence Imaging ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Live Cell ◽  
Imaging Probe ◽  
Cell Staining ◽  
Induced Pluripotent

Abstract 026: Optical Recording of Cardiac Action Potentials from Human Induced Pluripotent Stem Cell Derived Cardiomyocytes

2013 ◽  
Vol 113 (suppl_1) ◽  
Author(s):  
B Alexander Yi ◽  
Joel M Kralj ◽  
Adam E Cohen
Keyword(s):  
Stem Cell ◽  
High Throughput ◽  
Action Potentials ◽  
Pluripotent Stem Cell ◽  
Gene Mutations ◽  
Induced Pluripotent Stem Cell ◽  
Patch Clamping ◽  
Cardiac Action ◽  
Or Gene ◽  
Induced Pluripotent

The electrically excitable properties of cardiomyocytes stem from the activity of ion channels that allow the coordinated entry of ions to generate cardiac action potentials. Disruptions in ion channel function either by drugs or gene mutations can lead to cardiac arrhythmias. The ability to screen drugs or gene mutations rapidly for effects on the cardiac action potential would be of interest for both drug discovery as well as for studies of ion channel function; however, the time-consuming and technically challenging nature of conventional patch clamping can limit the ability to perform high throughput screens. Archaerhodopsin3, or Arch, is an Archaebacterial variant of the membrane protein bacteriorhodopsin that binds a retinal fluorophore whose signal is rapidly responsive to changes in membrane potential. Here, we report the use of Arch to optically record action potentials from human induced pluripotent stem cell-derived cardiomyocytes. Human induced pluripotent stem cells that stably express Arch were generated and then differentiated into cardiomyocytes. As compared to patch clamping, Arch faithfully reproduces many of the key features of cardiac action potentials and may be a tool to be used for high throughput electrophysiological screens of cardiomyocytes.

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