Reversal of Renal Fibrosis, Inflammation, and Glomerular Hypertrophy by Kallikrein Gene Delivery

2006 ◽  
Vol 17 (5) ◽  
pp. 545-555 ◽  
Author(s):  
Grant Bledsoe ◽  
Bo Shen ◽  
Yuyu Yao ◽  
Jenny J. Zhang ◽  
Lee Chao ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Renal Fibrosis ◽  
Glomerular Hypertrophy

Reversal of Renal Fibrosis, Inflammation, and Glomerular Hypertrophy by Kallikrein Gene Delivery

2006 ◽  
Vol 0 (0) ◽  
pp. 060801084750016
Author(s):  
Grant Bledsoe ◽  
Bo Shen ◽  
Yuyu Yao ◽  
Jenny J. Zhang ◽  
Lee Chao ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Renal Fibrosis ◽  
Glomerular Hypertrophy

Kallikrein gene transfer reduces renal fibrosis, hypertrophy, and proliferation in DOCA-salt hypertensive rats

2005 ◽  
Vol 289 (3) ◽  
pp. F622-F631 ◽  
Author(s):  
Chun-Fang Xia ◽  
Grant Bledsoe ◽  
Lee Chao ◽  
Julie Chao
Keyword(s):  
Gene Transfer ◽  
Gene Delivery ◽  
Renal Fibrosis ◽  
Transforming Growth Factor ◽  
Kinase Inhibitor ◽  
Glomerular Sclerosis ◽  
Protective Effects ◽  
Systemic Delivery ◽  
Hypertensive Rats ◽  
Tgf Β1

In DOCA-salt hypertension, renal kallikrein levels are increased and may play a protective role in renal injury. We investigated the effect of enhanced kallikrein levels on kidney remodeling of DOCA-salt hypertensive rats by systemic delivery of adenovirus containing human tissue kallikrein gene. Recombinant human kallikrein was detected in the urine and serum of rats after gene delivery. Kallikrein gene transfer significantly decreased DOCA- and salt-induced proteinuria, glomerular sclerosis, tubular dilatation, and luminal protein casts. Sirius red staining showed that kallikrein gene transfer reduced renal fibrosis, which was confirmed by decreased collagen I and fibronectin levels. Furthermore, kallikrein gene delivery diminished myofibroblast accumulation in the interstitium of the cortex and medulla, as well as transforming growth factor (TGF)-β1 immunostaining in glomeruli. Western blot analysis and ELISA verified the decrease in immunoreactive TGF-β1 levels. Kallikrein gene transfer also significantly reduced kidney weight, glomerular size, proliferating tubular epithelial cells, and macrophages/monocytes. Reduction of proliferation and hypertrophy was associated with reduced levels of the cyclin-dependent kinase inhibitor p27Kip1, and the phosphorylation of c-Jun NH2-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK). The protective effects of kallikrein were accompanied by increased urinary nitrate/nitrite and cGMP levels, and suppression of superoxide formation. These results indicate that kallikrein protects against mineralocorticoid-induced renal fibrosis glomerular hypertrophy, and renal cell proliferation via inhibition of oxidative stress, JNK/ERK activation, and p27Kip1 and TGF-β1 expression.

1004: In-Vivo Transfection of the Rat Corpus Cavernosum Using a Non-Viral, Linear Polyethylenimine Gene Delivery System: A Novel Application

2006 ◽  
Vol 175 (4S) ◽  
pp. 323-324 ◽  
Author(s):  
Joseph Dall'era ◽  
Sweaty Koul ◽  
Jesse Mills ◽  
Jeremy Myers ◽  
Randall B. Meacham ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Delivery System ◽  
Corpus Cavernosum ◽  
Gene Delivery System ◽  
System A ◽  
In Vivo Transfection ◽  
Linear Polyethylenimine

Agrobacterium-mediated gene delivery and the biology of host range limitations

1990 ◽  
Vol 79 (1) ◽  
pp. 135-139
Author(s):  
Andrew N. Binns
Keyword(s):  
Gene Delivery ◽  
Host Range

Rapid Optimization of Gene Delivery by Parallel End-modification of Poly(β-amino ester)s

2007 ◽  
Author(s):  
Gregory T Zugates ◽  
Weidan Peng ◽  
Andreas Zumbuehl ◽  
Siddharth Jhunjhunwala ◽  
Yu-Hung Huang ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Amino Ester

Targeted gene delivery to the cardiac tissue by adenovirus conjugated magnetic nanobeads

2011 ◽  
Vol 59 (S 01) ◽  
Author(s):  
Y Zhang ◽  
W Li ◽  
W Wang ◽  
L Ou ◽  
A Liebold ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Cardiac Tissue ◽  
Targeted Gene Delivery ◽  
Magnetic Nanobeads

βARKct gene delivery reverses atrophic gene regulation and enhances β-adrenergic response in the unloaded heart

2012 ◽  
Vol 60 (S 01) ◽  
Author(s):  
H Brinks ◽  
S Longnus ◽  
T Carrel ◽  
J Rabinowitz ◽  
WJ Koch ◽  
...  
Keyword(s):  
Gene Regulation ◽  
Gene Delivery ◽  
Adrenergic Response
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