Effective Inhibition of Newly Emerged A/H7N9 Virus with Oligonucleotides Targeted to Conserved Regions of the Virus Genome

2021 ◽  
pp. 1-7
Author(s):  
Marina Repkova ◽  
Asya Levina ◽  
Zinfer Ismagilov ◽  
Natalia Mazurkova ◽  
Oleg Mazurkov ◽  
...  
Keyword(s):  
Virus Genome ◽  
H7n9 Virus ◽  
Conserved Regions ◽  
Effective Inhibition

Implications of variations of "conserved" regions of hepatitis C virus genome

The Lancet ◽  
1995 ◽  
Vol 346 (8972) ◽  
pp. 425-426 ◽  
Author(s):  
J.Y.N Lau ◽  
P Simmonds ◽  
M.S Urdea
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Genome ◽  
Conserved Regions

scholarly journals The degree of attenuation of tick-borne encephalitis virus depends on the cumulative effects of point mutations

2001 ◽  
Vol 82 (7) ◽  
pp. 1667-1675 ◽  
Author(s):  
T. S. Gritsun ◽  
A. Desai ◽  
E. A. Gould
Keyword(s):  
Infectious Clone ◽  
Point Mutations ◽  
Virus Genome ◽  
Parental Virus ◽  
Variable Regions ◽  
Conserved Regions ◽  
Tick Borne Encephalitis ◽  
Virus Sequence ◽  
Tick Borne Encephalitis Virus ◽  
The Impact

An infectious clone (pGGVs) of the tick-borne encephalitis complex virus Vasilchenko (Vs) was constructed previously. Virus recovered from pGGVs produced slightly smaller plaques than the Vs parental virus. Sequence analysis demonstrated five nucleotide differences between the original Vs virus and pGGVs; four of these mutations resulted in amino acid substitutions, while the fifth mutation was located in the 3′ untranslated region (3′UTR). Two mutations were located in conserved regions and three mutations were located in variable regions of the virus genome. Reverse substitutions from the conserved regions of the genome, R496→H in the envelope (E) gene and C10884→T in the 3′UTR, were introduced both separately and together into the infectious clone and their biological effect on virus phenotype was evaluated. The engineered viruses with R496 in the E protein produced plaques of smaller size than viruses with H496 at this position. This mutation also affected the growth and neuroinvasiveness of the virus. In contrast, the consequence of a T10884→C substitution within the 3′UTR was noticeable only in cytotoxicity and neuroinvasiveness tests. However, all virus mutants engineered by modification of the infectious clone, including one with two wild-type mutations, H496 and T10884, showed reduced neuroinvasiveness in comparison with the Vs parental virus. Therefore, although the H496→R and T10884→C substitutions clearly reduce virus virulence, the other mutations within the variable regions of the capsid (I45→F) and the NS5 (T2688→A and M3385→I) genes also contribute to the process of attenuation. In terms of developing flavivirus vaccines, the impact of accumulating apparently minor mutations should be assessed in detail.

scholarly journals Design and in silico validation of polymerase chain reaction primers to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Maria Júlia P. Davi ◽  
Selma M. B. Jeronimo ◽  
João P. M. S. Lima ◽  
Daniel C. F. Lanza
Keyword(s):  
Polymerase Chain Reaction ◽  
Severe Acute Respiratory Syndrome ◽  
In Silico ◽  
False Negative ◽  
Pcr Primers ◽  
Virus Genome ◽  
Chain Reaction ◽  
Multiple Sequence ◽  
Conserved Regions ◽  
Polymerase Chain

AbstractAccurate designing of polymerase chain reaction (PCR) primers targeting conserved segments in viral genomes is desirable for preventing false-negative results and decreasing the need for standardization across different PCR protocols. In this work, we designed and described a set of primers and probes targeting conserved regions identified from a multiple sequence alignment of 2341 Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) genomes from the Global Initiative on Sharing All Influenza Data (GISAID). We subsequently validated those primers and probes in 211,833 SARS-CoV-2 whole-genome sequences. We obtained nine systems (forward primer + reverse primer + probe) that potentially anneal to highly conserved regions of the virus genome from these analyses. In silico predictions also demonstrated that those primers do not bind to nonspecific targets for human, bacterial, fungal, apicomplexan, and other Betacoronaviruses and less pathogenic sub-strains of coronavirus. The availability of these primer and probe sequences will make it possible to validate more efficient protocols for identifying SARS-CoV-2.

SUBTYPE A/H7N9 INFLUENZA: PATHOGENICITY AND PREVENTIVE MEASURES

2013 ◽  
pp. 95-100
Author(s):  
Dinh Binh Tran
Keyword(s):  
H7n9 Virus ◽  
Disease Symptoms ◽  
The People ◽  
High Risk Disease ◽  
Before And After ◽  
H7n9 Infection ◽  
Risk Of Disease ◽  
Tract Infections ◽  
Subtype A ◽  
Avian Populations

H7N9 is a serotype of the species Influenzavirus A. H7 virus normally circulates amongst avian populations with some variants known to occasionally infect humans. A H7N9 virus was first reported to have infected humans in 2013. The people with H7N9 virus are respiratory tract infections leading to pneumonia and can be death. To cope with H7N9 virus should strengthen supervision, strengthening management and treatment, epidemiological investigation and observe who has been exposed to the fatal cases. To limit the risk of disease, WHO recommends that people should be clean, safe eating, wash the hands often, especially before and after eating, after using the toilet, after contact with animals, after contact with sick people, use a mask when in contact with human or environmental exposure to the high-risk disease. Symptoms of influenza H7N9 infection are fever and cough then switch to pneumonia. Therefore, if having the symptoms like this, and then have the cough and shortness of breath, chest pain that should be early come the health facilities to diagnose and treat.

A Multivariate Data Analytics Approach Revealed No Footprint of APOBEC3 Proteins in Hepatitis B Virus Genome

2020 ◽  
Author(s):  
Hamid Alinejad-Rokny ◽  
Eisa Zarepour ◽  
Hoda Khadijeh Jahanian ◽  
Amin Beheshti ◽  
Abdollah Dehzangi
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Data Analytics ◽  
Multivariate Data ◽  
Virus Genome ◽  
B Virus ◽  
Apobec3 Proteins

Molecular characterization of SARS-CoV-2

2020 ◽  
Vol 20 ◽  
Author(s):  
Miribane Dërmaku-Sopjani ◽  
Mentor Sopjani
Keyword(s):  
Public Health ◽  
Drug Targets ◽  
Virus Genome ◽  
Health Crisis ◽  
Public Health Crisis ◽  
Therapeutic Drugs ◽  
New Public Health ◽  
New Public ◽  
And Function

Abstract:: The coronavirus disease 2019 (COVID-19) is currently a new public health crisis threatening the world. This pandemic disease is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The virus has been reported to be originated in bats and by yet unknown intermediary animals were transmitted to humans in China 2019. The SARSCoV- 2 spreads faster than its two ancestors the SARS-CoV and Middle East respiratory syndrome coronavirus (MERSCoV) but has reduced fatality. At present, the SARS-CoV-2 has caused about a 1.16 million of deaths with more than 43.4 million confirmed cases worldwide, resulting in a serious threat to public health globally with yet uncertain impact. The disease is transmitted by inhalation or direct contact with an infected person. The incubation period ranges from 1 to 14 days. COVID-19 is accompanied by various symptoms, including cough, fatigue. In most people the disease is mild, but in some other people, such as in elderly and people with chronic diseases, it may progress from pneumonia to a multi-organ dysfunction. Many people are reported asymptomatic. The virus genome is sequenced, but new variants are reported. Numerous biochemical aspects of its structure and function are revealed. To date, no clinically approved vaccines and/or specific therapeutic drugs are available to prevent or treat the COVID-19. However, there are reported intensive researches on the SARSCoV- 2 to potentially identify vaccines and/or drug targets, which may help to overcome the disease. In this review, we discuss recent advances in understanding the molecular structure of SARS-CoV-2 and its biochemical characteristics.

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