Positive Effect on Bone Fusion by the Combination of Platelet-Rich Plasma and a Gelatin β-Tricalcium Phosphate Sponge: A Study Using a Posterolateral Fusion Model of Lumbar Vertebrae in Rats

Abstract Background Bone substrates like hydroxyapatite and tricalcium phosphate have been widely used for promoting spinal fusion and reducing the complications caused by autograft. Whitlockite has been reported to promote better bone formation in rat calvaria models compare with them, but no study investigated its effect on spinal fusion yet. Also, the higher osteoinductivity of whitlockite raised concern of ectopic ossification, which was a complication of spinal fusion surgery that should be avoided. Methods In this study, we compared the osteoinductivity of whitlockite, hydroxyapatite, and tricalcium phosphate porous particles with SD rat spine posterolateral fusion model and investigated whether whitlockite could induce ectopic ossification with SD rat abdominal pouch model. Results The micro-CT result from the posterolateral fusion model showed whitlockite had slightly but significantly higher percent bone volume than tricalcium phosphate, though none of the materials formed successful fusion with surrounding bone tissue. The histology results showed the bone formed on the cortical surface of the transverse process but did not form a bridge between the processes. The result from the abdominal pouch model showed whitlockite did not induce ectopic bone formation. Conclusion Whitlockite had a potential of being a better bone substrate hydroxyapatite and tricalcium phosphate in spinal fusion with low risk of inducing ectopic ossification.

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Comparing Autograft, Allograft, and Tricalcium Phosphate Ceramic in a Goat Instrumented Posterolateral Fusion Model

Tissue Engineering Part C Methods ◽

10.1089/ten.tec.2012.0576 ◽

2013 ◽

Vol 19 (11) ◽

pp. 821-828 ◽

Cited By ~ 10

Author(s):

Diyar Delawi ◽

Moyo C. Kruyt ◽

Yuan Huipin ◽

Koen L. Vincken ◽

Joost D. de Bruijn ◽

...

Keyword(s):

Tricalcium Phosphate ◽

Posterolateral Fusion ◽

Fusion Model

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The Effect of Platelet-Rich Plasma on the Intra-Articular Microenvironment in Knee Osteoarthritis

International Journal of Molecular Sciences ◽

10.3390/ijms22115492 ◽

2021 ◽

Vol 22 (11) ◽

pp. 5492

Author(s):

Dawid Szwedowski ◽

Joanna Szczepanek ◽

Łukasz Paczesny ◽

Jan Zabrzyński ◽

Maciej Gagat ◽

...

Keyword(s):

Knee Osteoarthritis ◽

Operative Treatment ◽

Animal Studies ◽

Platelet Rich Plasma ◽

Treatment Options ◽

Treatment Protocols ◽

Metabolic Functions ◽

Inflammatory Drugs ◽

Positive Effect

Knee osteoarthritis (KOA) represents a clinical challenge due to poor potential for spontaneous healing of cartilage lesions. Several treatment options are available for KOA, including oral nonsteroidal anti-inflammatory drugs, physical therapy, braces, activity modification, and finally operative treatment. Intra-articular (IA) injections are usually used when the non-operative treatment is not effective, and when the surgery is not yet indicated. More and more studies suggesting that IA injections are as or even more efficient and safe than NSAIDs. Recently, research to improve intra-articular homeostasis has focused on biologic adjuncts, such as platelet-rich plasma (PRP). The catabolic and inflammatory intra-articular processes that exists in knee osteoarthritis (KOA) may be influenced by the administration of PRP and its derivatives. PRP can induce a regenerative response and lead to the improvement of metabolic functions of damaged structures. However, the positive effect on chondrogenesis and proliferation of mesenchymal stem cells (MSC) is still highly controversial. Recommendations from in vitro and animal research often lead to different clinical outcomes because it is difficult to translate non-clinical study outcomes and methodology recommendations to human clinical treatment protocols. In recent years, significant progress has been made in understanding the mechanism of PRP action. In this review, we will discuss mechanisms related to inflammation and chondrogenesis in cartilage repair and regenerative processes after PRP administration in in vitro and animal studies. Furthermore, we review clinical trials of PRP efficiency in changing the OA biomarkers in knee joint.

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Treatment of human periodontal infrabony defects with hydroxyapatite+ β tricalcium phosphate bone graft alone and in combination with platelet rich plasma: A randomized clinical trial

Indian Journal of Dental Research ◽

10.4103/0970-9290.90278 ◽

2011 ◽

Vol 22 (4) ◽

pp. 505 ◽

Cited By ~ 20

Author(s):

BharadwajT Kaushick ◽

O Padmalatha ◽

ND Jayakumar ◽

Sheeja Varghese

Keyword(s):

Clinical Trial ◽

Bone Graft ◽

Randomized Clinical Trial ◽

Tricalcium Phosphate ◽

Platelet Rich Plasma ◽

Infrabony Defects

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ESTIMATION OF THE EFFECT OF PLATELET RICH PLASMA PRODUCTS IN THE INTEGRATION OF POLYPROPYLENE MESH IMPLANT IN BIOLOGICAL TISSUES. EXPERIMENTAL MODEL IN RATS

EUREKA Health Sciences ◽

10.21303/2504-5679.2018.00736 ◽

2018 ◽

Vol 5 ◽

pp. 11-19

Author(s):

Dmitro Atanasov

Keyword(s):

Stem Cells ◽

Adipose Tissue ◽

Polypropylene Mesh ◽

Morphological Changes ◽

Platelet Rich Plasma ◽

Biological Tissues ◽

Tissue Reaction ◽

Plasma Cytokines ◽

Mesh Implant ◽

Positive Effect

The aim of the research is to determine morphological changes in the area of implantation of the polypropylene mesh implant and to determine the effect on the integration of the prosthesis of locally introduced adipose tissue and platelet rich plasma. Materials and methods. The experiment was performed on 36 sexually mature males of the Wistar line rats. The experiment simulated, studied and quantified local morphological responses and changes in developing in biological tissues that are in contact with implanted highly porous lightweight (80 g/m2) mesh implant in isolation and also in conditions of local administration of fatty graft and platelet rich plasma. Results: Assuming introduction of adipose tissue and platelet rich plasma in the zone of integration of mesh alloprosthesis under the influence of introduced regenerative cytokines as well as stromal stem cells activated by them there is an earlier activation of regenerative processes, enhanced angiogenesis which determines the optimal nature of the integration of the prosthesis with the formation of thin collagen fibers in more early terms minimizing excess peri-prosthetic fibrosis. Isolated introduction into the implantation zone of fatty suspension determines similar changes that have a slightly less pronounced character. These changes are quantitatively studied and the results obtained are statistically significant. Conclusions: Applying a fatty graft together with platelet rich plasma in the area of implantation of the lung polypropylene prosthesis, there was an accelerated tissue reaction from the integration of the prosthesis. Mesenchymal stem cells of adipose tissue that is a target for plasma cytokines enriched with thrombocytes have a more pronounced effect in stimulating reparative processes provided that they are simultaneously administered with PRP compared with isolated administration without PRP. The use of platelet rich plasma and adipose tissue design has a significant positive effect on local angiogenesis. Under conditions of improved angiogenesis and other stimulating factors in the conditions of introduction of adipose tissue and PRP, the integration of the prosthesis occurs with significantly lower peri-prosthetic fibrosis.

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