Chorioallantoic Membrane Assay as Model for Angiogenesis in Tissue Engineering: Focus on Stem Cells

The chick chorioallantoic membrane model has been around for over a century, applied in angiogenic, oncology, dental and xenograft research. Despite its often perceived archaic, redolent history, the chorioallantoic membrane assay offers new and exciting opportunities for material and growth factor evaluation in bone tissue engineering. Currently, superior/improved experimental methodology for the chorioallantoic membrane assay are difficult to identify, given an absence of scientific consensus in defining experimental approaches, including timing of inoculation with materials and the analysis of results. In addition, critically, regulatory and welfare issues impact upon experimental designs. Given such disparate points, this review details recent research using the ex vivo chorioallantoic membrane assay and the ex vivo organotypic culture to advance the field of bone tissue engineering, and highlights potential areas of improvement for their application based on recent developments within our group and the tissue engineering field.

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Controlling the in vitro differentiation of embryonic stem cells for myocardial tissue engineering applications

Cell Biology International ◽

10.1042/cbi20090021 ◽

2009 ◽

Author(s):

Dong-Bo Ou ◽

Rui Chen ◽

Xiong-Tao Liu ◽

Jing-Jing Guo ◽

Hong-Tao Wang ◽

...

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Embryonic Stem Cells ◽

Embryonic Stem ◽

Myocardial Tissue ◽

In Vitro Differentiation ◽

Engineering Applications ◽

Myocardial Tissue Engineering

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SYNTHESIS OF MICRO- AND NANOSIZED BIORESORBING SILICON-SUBSTITUTED TRICALCIUM PHOSPHATES FOR BONE TISSUE ENGINEERING AND THEIR BIOLOGICAL SAFETY USING MESENCHYMAL STEM CELLS

Nanomechanics Science and Technology An International Journal ◽

10.1615/nanomechanicsscitechnolintj.v6.i4.50 ◽

2015 ◽

Vol 6 (4) ◽

pp. 305-317

Author(s):

I. V. Fadeeva ◽

Ya. Yu. Filippov ◽

A. S. Fomin ◽

M. E. Shaposhnikov ◽

G. A. Davydova ◽

...

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Biological Safety ◽

Tricalcium Phosphates

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Faculty Opinions recommendation of Endochondral bone tissue engineering using embryonic stem cells.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1111103.574974 ◽

2008 ◽

Author(s):

Paolo Bianco ◽

Mara Riminucci

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Embryonic Stem Cells ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Embryonic Stem ◽

Endochondral Bone

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In vitro Three Dimensional Scaffold-free Construct of Human Adipose-derived Stem Cells in Coculture with Endothelial Cells and Fibroblasts

Revista de Chimie ◽

10.37358/rc.17.6.5670 ◽

2017 ◽

Vol 68 (6) ◽

pp. 1341-1344

Author(s):

Grigore Berea ◽

Gheorghe Gh. Balan ◽

Vasile Sandru ◽

Paul Dan Sirbu

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Endothelial Cells ◽

Single Cell ◽

Cell Line ◽

Bone Repair ◽

Umbilical Vein ◽

Human Fibroblasts ◽

Three Dimensional ◽

Adipose Derived Stem Cells

Complex interactions between stem cells, vascular cells and fibroblasts represent the substrate of building microenvironment-embedded 3D structures that can be grafted or added to bone substitute scaffolds in tissue engineering or clinical bone repair. Human Adipose-derived Stem Cells (hASCs), human umbilical vein endothelial cells (HUVECs) and normal dermal human fibroblasts (NDHF) can be mixed together in three dimensional scaffold free constructs and their behaviour will emphasize their potential use as seeding points in bone tissue engineering. Various combinations of the aforementioned cell lines were compared to single cell line culture in terms of size, viability and cell proliferation. At 5 weeks, viability dropped for single cell line spheroids while addition of NDHF to hASC maintained the viability at the same level at 5 weeks Fibroblasts addition to the 3D construct of stem cells and endothelial cells improves viability and reduces proliferation as a marker of cell differentiation toward osteogenic line.

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Mesenchymal Stem Cells Differentiation: Mitochondria Matter in Osteogenesis or Adipogenesis Direction

Current Stem Cell Research & Therapy ◽

10.2174/1574888x15666200324165655 ◽

2020 ◽

Vol 15 (7) ◽

pp. 602-606

Author(s):

Kun Ji ◽

Ling Ding ◽

Xi Chen ◽

Yun Dai ◽

Fangfang Sun ◽

...

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Therapeutic Potential ◽

Mesodermal Cell ◽

Cell Lineages ◽

Current Review ◽

Differentiation Factors ◽

The Relationship ◽

Msc Differentiation

Mesenchymal Stem Cells (MSCs) exhibit enormous therapeutic potential because of their indispensable regenerative, reparative, angiogenic, anti-apoptotic, and immunosuppressive properties. MSCs can best differentiate into mesodermal cell lineages, including osteoblasts, adipocytes, muscle cells, endothelial cells and chondrocytes. Specific differentiation of MSCs could be induced through limited conditions. In addition to the relevant differentiation factors, drastic changes also occur in the microenvironment to conduct it in an optimal manner for particular differentiation. Recent evidence suggests that the mitochondria participate in the regulating of direction and process of MSCs differentiation. Therefore, our current review focuses on how mitochondria participate in both osteogenesis and adipogenesis of MSC differentiation. Besides that, in our current review, we try to provide a further understanding of the relationship between the behavior of mitochondria and the direction of MSC differentiation, which could optimize current cellular culturing protocols for further facilitating tissue engineering by adjusting specific conditions of stem cells.

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Uncovering the Diversification of Tissue Engineering on the Emergent Areas of Stem Cells, Nanotechnology and Biomaterials

Current Stem Cell Research & Therapy ◽

10.2174/1574888x15666200103124821 ◽

2020 ◽

Vol 15 (3) ◽

pp. 187-201 ◽

Cited By ~ 1

Author(s):

Sunil K. Dubey ◽

Amit Alexander ◽

Munnangi Sivaram ◽

Mukta Agrawal ◽

Gautam Singhvi ◽

...

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Immune System ◽

Clinical Application ◽

Cell Types ◽

Patient Specific ◽

Potential Strategy ◽

Induced Pluripotent ◽

Treat All ◽

The Impact

Damaged or disabled tissue is life-threatening due to the lack of proper treatment. Many conventional transplantation methods like autograft, iso-graft and allograft are in existence for ages, but they are not sufficient to treat all types of tissue or organ damages. Stem cells, with their unique capabilities like self-renewal and differentiate into various cell types, can be a potential strategy for tissue regeneration. However, the challenges like reproducibility, uncontrolled propagation and differentiation, isolation of specific kinds of cell and tumorigenic nature made these stem cells away from clinical application. Today, various types of stem cells like embryonic, fetal or gestational tissue, mesenchymal and induced-pluripotent stem cells are under investigation for their clinical application. Tissue engineering helps in configuring the stem cells to develop into a desired viable tissue, to use them clinically as a substitute for the conventional method. The use of stem cell-derived Extracellular Vesicles (EVs) is being studied to replace the stem cells, which decreases the immunological complications associated with the direct administration of stem cells. Tissue engineering also investigates various biomaterials to use clinically, either to replace the bones or as a scaffold to support the growth of stemcells/ tissue. Depending upon the need, there are various biomaterials like bio-ceramics, natural and synthetic biodegradable polymers to support replacement or regeneration of tissue. Like the other fields of science, tissue engineering is also incorporating the nanotechnology to develop nano-scaffolds to provide and support the growth of stem cells with an environment mimicking the Extracellular matrix (ECM) of the desired tissue. Tissue engineering is also used in the modulation of the immune system by using patient-specific Mesenchymal Stem Cells (MSCs) and by modifying the physical features of scaffolds that may provoke the immune system. This review describes the use of various stem cells, biomaterials and the impact of nanotechnology in regenerative medicine.

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