Basic Fibroblast Growth Factor (Basic FGF) in Isolated Ovine Thyroid Follicles: Thyrotropin Stimulation and Effects of Basic FGF on DNA Synthesis, Iodine Uptake and Organification, and the Release of Insulin-Like Growth Factors (IGFs) and IGF-Binding Proteins

Thyroid ◽  
1994 ◽  
Vol 4 (1) ◽  
pp. 77-85 ◽  
Author(s):  
D.J. HILL ◽  
I.D. PHILLIPS ◽  
J.-F. WANG ◽  
G.P. BECKS
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Dna Synthesis ◽  
Fibroblast Growth Factor ◽  
Fibroblast Growth ◽  
Igf Binding Proteins ◽  
Basic Fgf ◽  
Igf Binding ◽  
Fibroblast Growth Factor Basic ◽  
Insulin Like Growth Factors

IGF-binding protein-6 is involved in growth inhibition in SH-SY5Y human neuroblastoma cells: its production is both IGF- and cell density-dependent

1997 ◽  
Vol 152 (2) ◽  
pp. 221-227 ◽  
Author(s):  
S Babajko ◽  
P Leneuve ◽  
C Loret ◽  
M Binoux
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Cell Density ◽  
Neuroblastoma Cells ◽  
Fibroblast Growth ◽  
Human Neuroblastoma ◽  
Igf System ◽  
Igf I ◽  
Igf Binding ◽  
Fibroblast Growth Factor Basic

Abstract The IGF system is involved in the growth and differentiation of neuroblastoma cells, but the precise roles played by the IGF-binding proteins (IGFBPs) remain unknown. We have examined the expression and functions of IGFBPs produced by the neuroblastoma cell line, SHSY5Y, in the presence of: insulin, IGF-I, IGF-II, des(1–3)IGF-I (an IGF-I analogue with weak affinity for IGFBPs), acidic fibroblast growth factor, basic fibroblast growth factor, or nerve growth factor. Under basal conditions, SH-SY5Y cells in serum-free medium secreted IGF-II, and traces of IGF-I, IGFBP-2 and IGFBP-4. After 24 h of culture, comparative mitogenic potencies were: des(1–3)IGF-I>IGF-1>IGF-II>insulin. After 48 h, when IGFBP-2 and IGFBP-4 concentrations in the culture media had increased, des(1–3)IGF-I remained the most active, but the activity of insulin now equalled or exceeded that of IGF-I and IGF-II. This suggests a negative feedback mechanism involving partial sequestration of IGF-I and IGF-II by IGFBP-2 and IGFBP-4. At high cell density and with high concentrations of IGF-I, des(1–3)IGF-I (40 ng/ml) or IGF-II (80 ng/ml), the mitogenic activities of the IGFs diminished concomitantly with the appearance in the culture medium of an additional IGFBP identified as IGFBP-6, whose production depended on activation of the type 1 IGF receptor. These findings suggest that IGFBP-6 contributes as an autocrine inhibitor in the regulation of growth by the IGF system in these neuroblastoma cells. Journal of Endocrinology (1997) 152, 221–227

scholarly journals The Role of Insulin-Like Growth Factors and Insulin-Like Growth Factor–Binding Proteins in the Nervous System

2019 ◽  
Vol 12 ◽  
pp. 117862641984217 ◽  
Author(s):  
Moira S Lewitt ◽  
Gary W Boyd
Keyword(s):  
Nervous System ◽  
Growth Factors ◽  
Growth Factor ◽  
Binding Proteins ◽  
Nervous System Development ◽  
Signalling Pathways ◽  
Igf Binding Proteins ◽  
Growth Factor Signalling ◽  
Igf Binding ◽  
Insulin Like Growth Factors

The insulin-like growth factors (IGF-I and IGF-II) and their receptors are widely expressed in nervous tissue from early embryonic life. They also cross the blood brain barriers by active transport, and their regulation as endocrine factors therefore differs from other tissues. In brain, IGFs have paracrine and autocrine actions that are modulated by IGF-binding proteins and interact with other growth factor signalling pathways. The IGF system has roles in nervous system development and maintenance. There is substantial evidence for a specific role for this system in some neurodegenerative diseases, and neuroprotective actions make this system an attractive target for new therapeutic approaches. In developing new therapies, interaction with IGF-binding proteins and other growth factor signalling pathways should be considered. This evidence is reviewed, gaps in knowledge are highlighted, and recommendations are made for future research.

Diverse forms of a receptor for acidic and basic fibroblast growth factors

1990 ◽  
Vol 10 (9) ◽  
pp. 4728-4736
Author(s):  
D E Johnson ◽  
P L Lee ◽  
J Lu ◽  
L T Williams
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Fibroblast Growth Factors ◽  
Cdna Clones ◽  
Fibroblast Growth ◽  
Fgf Receptor ◽  
Basic Fgf ◽  
Extracellular Region ◽  
Membrane Spanning

We recently reported the isolation of a chicken cDNA clone encoding a basic fibroblast growth factor (FGF) receptor that has three immunoglobulinlike domains in the extracellular region. We have now identified four unique human cDNA clones encoding previously unknown FGF receptor variants which contain only two immunoglobulinlike domains. Two of the human clones encode membrane-spanning receptors, and two encode putative secreted forms. Both the three- and two-immunoglobulinlike-domain forms mediate biological responsiveness to acidic and basic FGF. Thus, the first immunoglobulinlike domain of the three-domain form may have a function other than binding of acidic and basic FGF.

IGF-I increases bFGF-induced mitogenesis and upregulates FGFR-1 in rabbit vascular smooth muscle cells

1996 ◽  
Vol 270 (4) ◽  
pp. H1141-H1148 ◽  
Author(s):  
T. J. Reape ◽  
J. M. Kanczler ◽  
J. P. Ward ◽  
C. R. Thomas
Keyword(s):  
Smooth Muscle ◽  
Growth Factors ◽  
Growth Factor ◽  
Smooth Muscle Cells ◽  
Dna Synthesis ◽  
Fibroblast Growth Factor ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
Fibroblast Growth ◽  
Igf I

Insulin-like growth factor-I (IGF-I) and basic fibroblast growth factor (bFGF) have both been implicated in the abnormal proliferation of vascular smooth muscle cells (VSMC) that occurs after injury to the arterial wall in vivo. We have investigated the effects of these growth factors on proliferation of rabbit aortic smooth muscle cells (RASMC) in vitro. IGF-I, in contrast to bFGF, is a weak mitogen for RASMC. However, when IGF-I (10 ng/ml) was added in combination with bFGF for 24 h, the effect of the two growth factors on DNA synthesis was synergistic at all concentrations tested (P > 0.001 compared with summed values of bFGF alone plus IGF-I alone), and this synergy was also observed at the level of RASMC proliferation (P < 0.001). Time-course experiments indicated that although bFGF was able to stimulate DNA synthesis after 16 h, activity peaked at 24 h, and a synergistic response with IGF-I was not observed before 24 h. Northern blot analysis demonstrated that IGF-I (10 ng/ml) could selectively upregulate fibroblast growth factor receptor-1 (FGFR-1) mRNA 4.0 +/- 0.24-fold (P < 0.001) without a significant effect on FGFR-2, and this induction in FGFR-1 mRNA occurs in a time- and dose-dependent manner. In addition, IGF-I increases FGFR-1 protein levels in RASMC 2.7 +/- 0.12-fold (P < 0.01), as demonstrated by Western blotting, and this upregulation occurs before the peak in DNA synthesis. These results suggest that IGF-I may be capable of increasing the responsiveness of VSMC to bFGF through modulation of FGFR-1.

Acidic fibroblast growth factor (FGF) but not basic FGF induces sleep and fever in rabbits

1995 ◽  
Vol 269 (1) ◽  
pp. R87-R91 ◽  
Author(s):  
M. Knefati ◽  
C. Somogyi ◽  
L. Kapas ◽  
T. Bourcier ◽  
J. M. Krueger
Keyword(s):  
Growth Factor ◽  
Dna Synthesis ◽  
Fibroblast Growth Factor ◽  
Brain Temperature ◽  
Interleukin 1 ◽  
Fibroblast Growth ◽  
Sleep Regulation ◽  
Acidic Fibroblast Growth Factor ◽  
Basic Fgf ◽  
Functional Features

Acidic fibroblast growth factor (FGF) and basic FGF belong to a growth factor family. Interleukin-1, another member of that family, is involved in sleep regulation. FGFs and interleukin-1 share structural and functional features. We therefore determined whether acidic FGF and basic FGF were somnogenic. Male New Zealand White rabbits were provided with electroencephalographic (EEG) electrodes, a brain thermistor, and a lateral intracerebroventricular (icv) cannula. The animals were injected icv with isotonic NaCl (control) and on separate days with one of three doses of acidic or basic FGF (0.01, 0.1, or 1.0 micrograms) or with heat-treated acidic FGF (1.0 micrograms). The EEG, brain temperature, and motor activity were recorded for 23 h. The biological activity of basic FGF was determined in vitro by its ability to induce DNA synthesis in rat aortic smooth muscle cells. Acidic FGF induced prolonged dose-related increases in non-rapid eye movement sleep beginning in the 1st postinjection h and continuing for 12-23 h after the treatment. Acidic FGF also induced fevers of approximately 1 degree C after the 1.0 micrograms dose. Both activities of acidic FGF were lost after heat treatment. In contrast, basic FGF lacked somnogenic and pyrogenic activity, although it did induce DNA synthesis. Current results suggest that acidic FGF is part of the complex cytokine network in brain involved in sleep regulation.

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