Piezo1 regulates migration and invasion of breast cancer cells via modulating cell mechanobiological properties

2020 ◽  
Author(s):  
Yang Yu ◽  
Xiao’an Wu ◽  
Sisi Liu ◽  
Hongping Zhao ◽  
Bo Li ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Migration ◽  
Cancer Metastasis ◽  
Cell Stiffness ◽  
Migration And Invasion ◽  
Cell Migration And Invasion ◽  
Clinical Patient ◽  
Patient Database ◽  
Underlying Mechanisms

Abstract Cell migration and invasion are two essential processes during cancer metastasis. Increasing evidence has shown that the Piezo1 channel is involved in mediating cell migration and invasion in some types of cancers. However, the role of Piezo1 in the breast cancer and its underlying mechanisms have not been clarified yet. Here, we show that Piezo1 is high-expressed in breast cancer cell (BCC) lines, despite its complex expression in clinical patient database. Piezo1 knockdown (Piezo1-KD) promotes unconfined BCC migration, but impedes confined cell migration. Piezo1 may mediate BCC migration through the balances of cell adhesion, cell stiffness, and contractility. Furthermore, Piezo1-KD inhibits BCC invasion by impairing the invadopodium formation and suppressing the expression of metalloproteinases (MMPs) as well. However, the proliferation and cell cycle of BCCs are not significantly affected by Piezo1. Our study highlights a crucial role of Piezo1 in regulating migration and invasion of BCCs, indicating Piezo1 channel might be a new prognostic and therapeutic target in BCCs.

Circular RNA circVAPA regulates breast cancer cell migration and invasion via sponging miR-130a-5p

Epigenomics ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 303-317 ◽  
Author(s):  
Si-ying Zhou ◽  
Wei Chen ◽  
Su-jin Yang ◽  
Jian Li ◽  
Jun-ying Zhang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Migration ◽  
Circular Rna ◽  
Migration And Invasion ◽  
Mirna Sponge ◽  
Cell Migration And Invasion ◽  
Cancer Migration ◽  
Cancer Tissues ◽  
The Relationship

Aim: We aimed to explore the roles of circular RNA, circVAPA in regulating cell migration and invasion of breast cancer. Materials & methods: CircVAPA expression was detected in breast cancer tissues and cells. The role of circVAPA was evaluated by MTT assay, wound-healing and transwell assay. The relationship between circVAPA and miR-130a-5p and the location of circVAPA were explored. Results: We discovered that circVAPA was dysregulated in breast cancer tissues and cells. Ectopic circVAPA regulated breast cancer migration, invasion and proliferation. CircVAPA was mainly expressed in the cytoplasm and could act as a miRNA sponge for miR-130a-5p, but did not regulate its parental gene. Conclusion: CircVAPA may promote migration and invasion capacity of breast cancer via harboring miR-130a-5p.

scholarly journals Long noncoding RNA TTN-AS1 promotes breast cancer cell migration and invasion via sponging miR-140-5p

2020 ◽  
Author(s):  
Yusheng Li ◽  
Fan Wang
Keyword(s):  
Breast Cancer ◽  
Cell Migration ◽  
Noncoding Rna ◽  
Vital Role ◽  
Biological Behavior ◽  
Migration And Invasion ◽  
Behavior Changes ◽  
Cell Migration And Invasion ◽  
Rna Immunoprecipitation

Abstract Objectives Breast cancer (BC) is one of the most ordinary fatal cancers. Recent studies have identified the vital role of long noncoding RNAs (lncRNAs) in the development and progression of BC. In this research, lncRNA TTN-AS1 was studied to identify how it functioned in the metastasis of BC.Methods TTN-AS1 expression of tissues was detected by RT-qPCR in 56 BC patients. Wound healing assay and transwell assay were used to observe the biological behavior changes of BC cells through gain or loss of TTN-AS1. In addition, luciferase assays and RNA immunoprecipitation (RIP) assay were performed to discover the potential targets of TTN-AS1 in BC cells.Results TTN-AS1 expression level in BC samples was higher than that of adjacent ones. Besides, cell migrated ability and cell invaded ability of BC cells were inhibited after TTN-AS1 was silenced. Cell migrated ability and cell invaded ability of BC cells were promoted after TTN-AS1 was overexpressed. In addition, miR-140-5p was upregulated after silence of TTN-AS1 in BC cells, while miR-140-5p was downregulated after overexpression of TTN-AS1 in BC cells. Furthermore, luciferase assays and RNA immunoprecipitation assay (RIP) showed that miR-140-5p was a direct target of TTN-AS1 in BC.Conclusion Our study uncovers a new oncogene in BC and suggests that TTN-AS1 could enhance BC cell migration and invasion via sponging miR-140-5p, which provides a novel therapeutic target for BC patients.

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