Method of Temporarily Preserving Fresh Frozen Sections Stained with Polychrome Methylene Blue

1936 ◽  
Vol 6 (2) ◽  
pp. 195-195
Author(s):  
James W. Kernohan
Keyword(s):  
Methylene Blue ◽  
Frozen Sections ◽  
Fresh Frozen

scholarly journals p-HYDROQUINONE AND p-BENZOQUINONE AS HISTOCHEMICAL REAGENTS I. A NEW TETRAZOLIUM METHOD FOR AMINO GROUPS, AND THE MECHANISM OF FORMAZAN STAINING OF PARAFFIN AND FRESH FROZEN SECTIONS

1967 ◽  
Vol 15 (7) ◽  
pp. 404-408 ◽  
Author(s):  
G. G. CARMICHAEL ◽  
STEPHANIE T. K. MANDER
Keyword(s):  
Electron Acceptor ◽  
Thermal Shrinkage ◽  
Frozen Sections ◽  
Amino Groups ◽  
Paraffin Sections ◽  
Tetrazolium Bromide ◽  
Acid Ph ◽  
Reduction System ◽  
Oxidation Reduction ◽  
Fresh Frozen

The staining of amino groups by formazan when dehydrated paraffin sections are incubated in a mixture of hydroquinone and 3-(4,5-dimethyl thiazolyl-2)-2 ,5-diphenyl-2H-tetrazolium bromide at an acid pH is reported. The mechanism of this reaction and of the cytoplasmic deposition of formazan in fresh frozen sections incubated under similar conditions is investigated. It is shown that the oxidation of hydroquinone to semiquinone is responsible for the reaction, the tetrazole acting as electron acceptor. The tissue amino groups, exposed by dehydration and thermal shrinkage, and the nitrogen groupings of phosphobipid behave as "catalysts." The relevant properties of the hydroquinone-benzoquinone oxidation-reduction system are described, and the reactions between benzoquinone and tissue constituents are reviewed.

Effect of bacterial endotoxemia on succinic dehydrogenase of liver and kidney of rabbit

1961 ◽  
Vol 201 (1) ◽  
pp. 16-18 ◽  
Author(s):  
J. Cascarano ◽  
A. D. Rubin ◽  
A. K. Neumann ◽  
B. W. Zweifach
Keyword(s):  
Succinic Dehydrogenase ◽  
Significant Inhibition ◽  
Frozen Sections ◽  
Experimental Animals ◽  
E Coli ◽  
Fresh Frozen ◽  
Tissue Homogenates ◽  
Liver And Kidney ◽  
Lethal Doses

The in vivo inhibition of liver and kidney succinic dehydrogenase by administration of lethal doses of bacterial endotoxin ( Escherichia coli and Salmonella typhosa) was investigated. Quantitative determinations conducted on tissue homogenates revealed significant inhibition of activity only in liver of rabbits injected with E. coli lipopolysaccharide. The histochemical distribution of succinic dehydrogenase in fresh frozen sections of kidney was the same in both control and experimental animals. However, the centrolobular areas of liver appeared considerably depressed in activity in both E. coli and S. typhosa endotoxin-treated animals. These data, along with those presented by other studies in the literature, suggest that the action of endotoxin appears to be restricted to certain cells.

Lipoproteins and Fibrinogen in Atherogenesis

1979 ◽  
Author(s):  
K.W. Walton
Keyword(s):  
Low Density Lipoproteins ◽  
Atherosclerotic Plaques ◽  
Low Density ◽  
Frozen Sections ◽  
Fibrin Monomer ◽  
Fresh Frozen ◽  
Arterial Intima

Previous work from this and other laboratories has shown that material antigenically related to the low-density lipoproteins (LDL) and to fibrinogen is demonstrable in atherosclerotic plaques. When arterial intima is extracted electrophoretically, ‘bound’ and ‘labile’ fractions of these antigens are in each case demonstrable. In the case of the “bound’ fraction of fibrinogen-related antigen (FRA) it has not been clear as to whether the material is in the form of native fibrinogen, as fibrin monomer, or as fibrin. To examine this problem fresh-frozen sections of arteries containing FRA have been examined by immunohistological techniques using antisera specific for fibrinogen, fibrinopeptides, plasmin and fibronectin. The results obtained with these antisera will be compared with one another and with results obtained with antisera to the antigens of LDL.

scholarly journals Granulomatous Pancreas: A Case Report of Pancreatic Sarcoid

2017 ◽  
Vol 2017 ◽  
pp. 1-3 ◽  
Author(s):  
Tatiana Bihun ◽  
Yanet Diaz ◽  
Seth Wenig
Keyword(s):  
Disease Process ◽  
Unknown Etiology ◽  
Frozen Sections ◽  
Gi Tract ◽  
Quadrant Pain ◽  
Pancreatic Involvement ◽  
Pancreatic Masses ◽  
Fresh Frozen ◽  
History Of ◽  
Head Of The Pancreas

Sarcoidosis is a chronic, systemic, noncaseating granulomatous disease process of unknown etiology. Sarcoidosis most commonly manifests in the lungs; however, gastrointestinal manifestations can occur. If in the GI tract, it is almost always found in the liver. Solitary pancreatic lesions are extremely rare, with less than 50 documented cases found in the literature. We present a case of a 61-year-old female, with a past medical history of sarcoidosis, who presented to the ER with unexpected weight loss, scleral icterus, right upper quadrant pain, and epigastric and back pain. US and MRI found a dilated common bile duct and mild dilation of the pancreatic duct, as well as a focal prominence in the head of the pancreas surrounded by areas of atrophy. A pancreaticoduodenectomy procedure was performed and fresh frozen sections were taken. The pathologist made a diagnosis of nonnecrotizing granulomatous pancreatitis. Pancreatic sarcoid is often asymptomatic and a benign finding on autopsy; however, clinicians should be mindful of pancreatic involvement when working up differential diagnosis for pancreatic masses.

The Histochemical Localization of Cholesterol in Formalin-Fixed and Fresh Frozen Sections

1976 ◽  
Vol 51 (3) ◽  
pp. 143-145 ◽  
Author(s):  
D. J. Rossouw ◽  
Carol C. Chase ◽  
Ina Raath ◽  
F. M. Engelbrecht
Keyword(s):  
Histochemical Localization ◽  
Frozen Sections ◽  
Fresh Frozen ◽  
Formalin Fixed

scholarly journals Use of low temperatures for glutathione histochemical stain.

1983 ◽  
Vol 31 (7) ◽  
pp. 975-976 ◽  
Author(s):  
P Chieco ◽  
P J Boor
Keyword(s):  
Low Temperature ◽  
Standard Method ◽  
Low Temperatures ◽  
Frozen Sections ◽  
Mercury Orange ◽  
Fresh Frozen ◽  
In Cells

We performed glutathione (GSH) staining at a low temperature to prevent GSH release from the section and, hence, improve morphology. Fresh frozen sections of liver, lung, kidney, heart, and stomach were incubated for GSH activity in an ice bath (2-5 degrees C) for 5-10 min. Low temperature incubation prevented GSH diffusion out of cells and minimized migration of granules into vessels and outside of tissue. Incubation at low temperature generally reduced the intensity of the stain compared to the standard method. We conclude that low temperature incubation improves GSH localization in cells, probably by regulating the rate, formation, and the size of GSH-mercury orange complexes.

scholarly journals Freeze substituted tissue in 5'-nucleotidase histochemistry. Comparative histochemical and biochemical investigations.

1979 ◽  
Vol 27 (12) ◽  
pp. 1582-1587 ◽  
Author(s):  
K Klaushofer ◽  
H von Mayersbach
Keyword(s):  
Enzyme Activity ◽  
Sensitivity And Specificity ◽  
Rat Liver ◽  
Freeze Substitution ◽  
High Sensitivity ◽  
Histochemical Demonstration ◽  
Frozen Sections ◽  
Enzyme Localization ◽  
Fresh Frozen ◽  
Preparation Techniques

The suitability of freeze-substitution in n-butanol and paraffin embedding of tissues for the histochemical demonstration of 5'-nucleotidase was investigated and compared with commonly used preparation techniques, such as fresh frozen sections and cryostate sections of cold formalin and glutaraldehyde-fixed rat liver. The influences of each step of the preparation techniques on the enzyme activity were controlled by a quantitative radiochemical assay. Freeze substitution was revealed to be superior to the commonly used preparation techniques with respect to: 1) high sensitivity and specificity of the histochemical 5'-nucleotidase reaction (this is based on the fact that incubation media with very low lead concentrations (0,6 mM/1) can be used); 2) excellent morphological appearance of the tissues showing cytological details of enzyme localization; 3) unlimited storage of the tissue materials and ease of sectioning.

Fresh-Frozen Sections in Head and Neck Surgery

1963 ◽  
Vol 43 (4) ◽  
pp. 943-950 ◽  
Author(s):  
Edgar G. Harrison ◽  
Lewis B. Woolner
Keyword(s):  
Head And Neck ◽  
Neck Surgery ◽  
Head And Neck Surgery ◽  
Frozen Sections ◽  
Fresh Frozen
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